Amino acid dipepetide frequency for Halanaerobium sp. DL-01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.426AlaAla: 7.426 ± 0.133
0.48AlaCys: 0.48 ± 0.03
4.627AlaAsp: 4.627 ± 0.084
7.157AlaGlu: 7.157 ± 0.142
2.566AlaPhe: 2.566 ± 0.066
5.859AlaGly: 5.859 ± 0.109
1.02AlaHis: 1.02 ± 0.039
3.82AlaIle: 3.82 ± 0.084
3.839AlaLys: 3.839 ± 0.076
6.223AlaLeu: 6.223 ± 0.112
1.44AlaMet: 1.44 ± 0.044
2.274AlaAsn: 2.274 ± 0.059
1.693AlaPro: 1.693 ± 0.054
1.754AlaGln: 1.754 ± 0.044
2.756AlaArg: 2.756 ± 0.059
3.423AlaSer: 3.423 ± 0.066
2.065AlaThr: 2.065 ± 0.056
6.917AlaVal: 6.917 ± 0.121
0.373AlaTrp: 0.373 ± 0.024
2.036AlaTyr: 2.036 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.42CysAla: 0.42 ± 0.026
0.106CysCys: 0.106 ± 0.015
0.393CysAsp: 0.393 ± 0.026
0.459CysGlu: 0.459 ± 0.028
0.225CysPhe: 0.225 ± 0.018
0.802CysGly: 0.802 ± 0.041
0.169CysHis: 0.169 ± 0.014
0.48CysIle: 0.48 ± 0.028
0.416CysLys: 0.416 ± 0.025
0.548CysLeu: 0.548 ± 0.026
0.144CysMet: 0.144 ± 0.014
0.308CysAsn: 0.308 ± 0.02
0.417CysPro: 0.417 ± 0.029
0.209CysGln: 0.209 ± 0.018
0.449CysArg: 0.449 ± 0.026
0.547CysSer: 0.547 ± 0.028
0.327CysThr: 0.327 ± 0.024
0.371CysVal: 0.371 ± 0.027
0.042CysTrp: 0.042 ± 0.007
0.219CysTyr: 0.219 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
2.8AspAla: 2.8 ± 0.071
0.452AspCys: 0.452 ± 0.027
3.007AspAsp: 3.007 ± 0.068
4.348AspGlu: 4.348 ± 0.085
3.077AspPhe: 3.077 ± 0.072
3.333AspGly: 3.333 ± 0.079
0.869AspHis: 0.869 ± 0.033
6.085AspIle: 6.085 ± 0.095
5.298AspLys: 5.298 ± 0.088
6.24AspLeu: 6.24 ± 0.088
1.531AspMet: 1.531 ± 0.044
3.205AspAsn: 3.205 ± 0.067
1.953AspPro: 1.953 ± 0.05
1.606AspGln: 1.606 ± 0.045
2.544AspArg: 2.544 ± 0.066
3.3AspSer: 3.3 ± 0.076
2.034AspThr: 2.034 ± 0.056
3.253AspVal: 3.253 ± 0.071
0.484AspTrp: 0.484 ± 0.03
2.898AspTyr: 2.898 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
5.08GluAla: 5.08 ± 0.106
0.331GluCys: 0.331 ± 0.022
4.704GluAsp: 4.704 ± 0.093
8.079GluGlu: 8.079 ± 0.137
3.134GluPhe: 3.134 ± 0.06
4.132GluGly: 4.132 ± 0.079
1.058GluHis: 1.058 ± 0.041
8.369GluIle: 8.369 ± 0.129
9.518GluLys: 9.518 ± 0.129
7.665GluLeu: 7.665 ± 0.101
2.415GluMet: 2.415 ± 0.054
5.291GluAsn: 5.291 ± 0.086
1.598GluPro: 1.598 ± 0.051
1.959GluGln: 1.959 ± 0.052
2.978GluArg: 2.978 ± 0.071
3.487GluSer: 3.487 ± 0.076
2.895GluThr: 2.895 ± 0.066
5.288GluVal: 5.288 ± 0.09
0.462GluTrp: 0.462 ± 0.03
2.784GluTyr: 2.784 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
2.922PheAla: 2.922 ± 0.058
0.346PheCys: 0.346 ± 0.023
2.493PheAsp: 2.493 ± 0.059
2.593PheGlu: 2.593 ± 0.064
2.29PhePhe: 2.29 ± 0.07
2.879PheGly: 2.879 ± 0.062
0.583PheHis: 0.583 ± 0.028
4.049PheIle: 4.049 ± 0.103
3.467PheLys: 3.467 ± 0.078
4.507PheLeu: 4.507 ± 0.093
1.017PheMet: 1.017 ± 0.038
2.591PheAsn: 2.591 ± 0.061
1.444PhePro: 1.444 ± 0.047
1.053PheGln: 1.053 ± 0.032
1.418PheArg: 1.418 ± 0.049
3.656PheSer: 3.656 ± 0.08
2.262PheThr: 2.262 ± 0.06
2.625PheVal: 2.625 ± 0.068
0.347PheTrp: 0.347 ± 0.025
1.712PheTyr: 1.712 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
4.494GlyAla: 4.494 ± 0.106
0.646GlyCys: 0.646 ± 0.034
3.439GlyAsp: 3.439 ± 0.073
4.884GlyGlu: 4.884 ± 0.084
3.119GlyPhe: 3.119 ± 0.067
4.914GlyGly: 4.914 ± 0.106
1.157GlyHis: 1.157 ± 0.041
6.361GlyIle: 6.361 ± 0.115
5.078GlyLys: 5.078 ± 0.084
6.213GlyLeu: 6.213 ± 0.104
1.849GlyMet: 1.849 ± 0.062
2.717GlyAsn: 2.717 ± 0.061
1.693GlyPro: 1.693 ± 0.053
1.709GlyGln: 1.709 ± 0.047
2.98GlyArg: 2.98 ± 0.064
4.197GlySer: 4.197 ± 0.077
3.304GlyThr: 3.304 ± 0.073
4.667GlyVal: 4.667 ± 0.107
0.569GlyTrp: 0.569 ± 0.028
2.686GlyTyr: 2.686 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
0.822HisAla: 0.822 ± 0.031
0.153HisCys: 0.153 ± 0.015
0.73HisAsp: 0.73 ± 0.037
0.769HisGlu: 0.769 ± 0.038
0.738HisPhe: 0.738 ± 0.033
1.017HisGly: 1.017 ± 0.035
0.36HisHis: 0.36 ± 0.026
1.345HisIle: 1.345 ± 0.043
1.04HisLys: 1.04 ± 0.035
1.503HisLeu: 1.503 ± 0.048
0.279HisMet: 0.279 ± 0.021
0.777HisAsn: 0.777 ± 0.029
0.839HisPro: 0.839 ± 0.032
0.494HisGln: 0.494 ± 0.023
0.67HisArg: 0.67 ± 0.035
0.97HisSer: 0.97 ± 0.039
0.636HisThr: 0.636 ± 0.028
0.751HisVal: 0.751 ± 0.028
0.088HisTrp: 0.088 ± 0.011
0.674HisTyr: 0.674 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.964IleAla: 5.964 ± 0.097
0.639IleCys: 0.639 ± 0.032
5.807IleAsp: 5.807 ± 0.104
6.91IleGlu: 6.91 ± 0.096
4.409IlePhe: 4.409 ± 0.11
5.641IleGly: 5.641 ± 0.108
1.077IleHis: 1.077 ± 0.042
9.039IleIle: 9.039 ± 0.149
8.034IleLys: 8.034 ± 0.126
8.211IleLeu: 8.211 ± 0.127
2.145IleMet: 2.145 ± 0.066
5.244IleAsn: 5.244 ± 0.089
3.453IlePro: 3.453 ± 0.069
1.723IleGln: 1.723 ± 0.052
3.142IleArg: 3.142 ± 0.067
6.307IleSer: 6.307 ± 0.102
4.489IleThr: 4.489 ± 0.088
5.189IleVal: 5.189 ± 0.088
0.511IleTrp: 0.511 ± 0.028
3.155IleTyr: 3.155 ± 0.066
0.0IleXaa: 0.0 ± 0.0
Lys
5.163LysAla: 5.163 ± 0.09
0.455LysCys: 0.455 ± 0.028
4.557LysAsp: 4.557 ± 0.086
8.46LysGlu: 8.46 ± 0.122
3.089LysPhe: 3.089 ± 0.069
4.478LysGly: 4.478 ± 0.089
1.067LysHis: 1.067 ± 0.044
8.725LysIle: 8.725 ± 0.124
9.626LysLys: 9.626 ± 0.155
7.66LysLeu: 7.66 ± 0.113
2.339LysMet: 2.339 ± 0.055
6.424LysAsn: 6.424 ± 0.104
1.833LysPro: 1.833 ± 0.05
1.973LysGln: 1.973 ± 0.05
3.174LysArg: 3.174 ± 0.07
4.242LysSer: 4.242 ± 0.091
3.957LysThr: 3.957 ± 0.071
4.825LysVal: 4.825 ± 0.096
0.494LysTrp: 0.494 ± 0.026
3.491LysTyr: 3.491 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
6.73LeuAla: 6.73 ± 0.115
0.511LeuCys: 0.511 ± 0.026
5.678LeuAsp: 5.678 ± 0.095
7.32LeuGlu: 7.32 ± 0.105
4.053LeuPhe: 4.053 ± 0.098
6.05LeuGly: 6.05 ± 0.119
1.277LeuHis: 1.277 ± 0.044
8.661LeuIle: 8.661 ± 0.128
9.425LeuLys: 9.425 ± 0.126
8.962LeuLeu: 8.962 ± 0.14
2.288LeuMet: 2.288 ± 0.059
5.842LeuAsn: 5.842 ± 0.106
3.526LeuPro: 3.526 ± 0.074
2.509LeuGln: 2.509 ± 0.057
3.297LeuArg: 3.297 ± 0.074
6.446LeuSer: 6.446 ± 0.102
4.846LeuThr: 4.846 ± 0.087
4.987LeuVal: 4.987 ± 0.077
0.54LeuTrp: 0.54 ± 0.027
2.753LeuTyr: 2.753 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
2.11MetAla: 2.11 ± 0.055
0.124MetCys: 0.124 ± 0.011
1.444MetAsp: 1.444 ± 0.042
1.924MetGlu: 1.924 ± 0.055
0.832MetPhe: 0.832 ± 0.037
1.789MetGly: 1.789 ± 0.049
0.318MetHis: 0.318 ± 0.022
2.135MetIle: 2.135 ± 0.054
2.097MetLys: 2.097 ± 0.049
2.359MetLeu: 2.359 ± 0.07
0.682MetMet: 0.682 ± 0.035
1.24MetAsn: 1.24 ± 0.044
0.9MetPro: 0.9 ± 0.042
0.787MetGln: 0.787 ± 0.034
1.007MetArg: 1.007 ± 0.04
1.549MetSer: 1.549 ± 0.047
1.206MetThr: 1.206 ± 0.045
1.575MetVal: 1.575 ± 0.046
0.131MetTrp: 0.131 ± 0.012
0.593MetTyr: 0.593 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.479AsnAla: 2.479 ± 0.066
0.409AsnCys: 0.409 ± 0.025
2.673AsnAsp: 2.673 ± 0.06
3.289AsnGlu: 3.289 ± 0.077
2.796AsnPhe: 2.796 ± 0.07
2.82AsnGly: 2.82 ± 0.062
0.72AsnHis: 0.72 ± 0.034
5.845AsnIle: 5.845 ± 0.108
5.011AsnLys: 5.011 ± 0.092
5.838AsnLeu: 5.838 ± 0.083
1.433AsnMet: 1.433 ± 0.045
3.652AsnAsn: 3.652 ± 0.084
2.136AsnPro: 2.136 ± 0.052
1.582AsnGln: 1.582 ± 0.051
2.136AsnArg: 2.136 ± 0.052
3.378AsnSer: 3.378 ± 0.077
2.209AsnThr: 2.209 ± 0.061
2.696AsnVal: 2.696 ± 0.069
0.455AsnTrp: 0.455 ± 0.025
2.68AsnTyr: 2.68 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
2.418ProAla: 2.418 ± 0.06
0.232ProCys: 0.232 ± 0.018
2.227ProAsp: 2.227 ± 0.06
3.384ProGlu: 3.384 ± 0.073
1.552ProPhe: 1.552 ± 0.046
2.555ProGly: 2.555 ± 0.062
0.673ProHis: 0.673 ± 0.03
2.224ProIle: 2.224 ± 0.058
1.648ProLys: 1.648 ± 0.048
2.893ProLeu: 2.893 ± 0.062
0.684ProMet: 0.684 ± 0.034
1.063ProAsn: 1.063 ± 0.037
0.952ProPro: 0.952 ± 0.037
0.89ProGln: 0.89 ± 0.038
1.055ProArg: 1.055 ± 0.031
1.648ProSer: 1.648 ± 0.051
1.432ProThr: 1.432 ± 0.048
2.687ProVal: 2.687 ± 0.068
0.264ProTrp: 0.264 ± 0.018
1.275ProTyr: 1.275 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
1.835GlnAla: 1.835 ± 0.059
0.153GlnCys: 0.153 ± 0.016
1.277GlnAsp: 1.277 ± 0.048
2.061GlnGlu: 2.061 ± 0.064
0.985GlnPhe: 0.985 ± 0.038
1.525GlnGly: 1.525 ± 0.039
0.391GlnHis: 0.391 ± 0.025
2.461GlnIle: 2.461 ± 0.061
2.665GlnLys: 2.665 ± 0.071
2.643GlnLeu: 2.643 ± 0.065
0.66GlnMet: 0.66 ± 0.033
1.645GlnAsn: 1.645 ± 0.053
0.79GlnPro: 0.79 ± 0.033
0.984GlnGln: 0.984 ± 0.037
1.088GlnArg: 1.088 ± 0.039
1.439GlnSer: 1.439 ± 0.043
1.148GlnThr: 1.148 ± 0.039
1.501GlnVal: 1.501 ± 0.052
0.181GlnTrp: 0.181 ± 0.015
0.892GlnTyr: 0.892 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.541ArgAla: 2.541 ± 0.06
0.331ArgCys: 0.331 ± 0.022
2.347ArgAsp: 2.347 ± 0.06
3.619ArgGlu: 3.619 ± 0.066
1.599ArgPhe: 1.599 ± 0.053
2.655ArgGly: 2.655 ± 0.071
0.578ArgHis: 0.578 ± 0.029
3.347ArgIle: 3.347 ± 0.069
3.547ArgLys: 3.547 ± 0.077
3.575ArgLeu: 3.575 ± 0.084
1.056ArgMet: 1.056 ± 0.042
1.892ArgAsn: 1.892 ± 0.057
1.186ArgPro: 1.186 ± 0.044
1.141ArgGln: 1.141 ± 0.041
1.822ArgArg: 1.822 ± 0.06
2.22ArgSer: 2.22 ± 0.063
1.751ArgThr: 1.751 ± 0.044
2.354ArgVal: 2.354 ± 0.062
0.294ArgTrp: 0.294 ± 0.02
1.457ArgTyr: 1.457 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
4.208SerAla: 4.208 ± 0.087
0.532SerCys: 0.532 ± 0.032
3.544SerAsp: 3.544 ± 0.071
4.572SerGlu: 4.572 ± 0.08
2.93SerPhe: 2.93 ± 0.068
4.861SerGly: 4.861 ± 0.078
0.954SerHis: 0.954 ± 0.035
4.948SerIle: 4.948 ± 0.084
4.039SerLys: 4.039 ± 0.073
5.955SerLeu: 5.955 ± 0.098
1.324SerMet: 1.324 ± 0.046
2.426SerAsn: 2.426 ± 0.065
1.86SerPro: 1.86 ± 0.05
1.758SerGln: 1.758 ± 0.053
3.004SerArg: 3.004 ± 0.065
4.296SerSer: 4.296 ± 0.094
2.625SerThr: 2.625 ± 0.059
3.608SerVal: 3.608 ± 0.076
0.615SerTrp: 0.615 ± 0.032
2.104SerTyr: 2.104 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.507ThrAla: 4.507 ± 0.09
0.275ThrCys: 0.275 ± 0.02
2.817ThrAsp: 2.817 ± 0.062
3.329ThrGlu: 3.329 ± 0.079
1.785ThrPhe: 1.785 ± 0.058
4.081ThrGly: 4.081 ± 0.092
0.744ThrHis: 0.744 ± 0.036
3.481ThrIle: 3.481 ± 0.084
2.523ThrLys: 2.523 ± 0.065
3.914ThrLeu: 3.914 ± 0.075
0.901ThrMet: 0.901 ± 0.035
1.746ThrAsn: 1.746 ± 0.049
1.793ThrPro: 1.793 ± 0.049
0.963ThrGln: 0.963 ± 0.037
1.542ThrArg: 1.542 ± 0.054
2.35ThrSer: 2.35 ± 0.061
2.171ThrThr: 2.171 ± 0.058
3.444ThrVal: 3.444 ± 0.072
0.28ThrTrp: 0.28 ± 0.021
1.36ThrTyr: 1.36 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
3.647ValAla: 3.647 ± 0.084
0.518ValCys: 0.518 ± 0.029
4.097ValAsp: 4.097 ± 0.082
5.449ValGlu: 5.449 ± 0.105
2.812ValPhe: 2.812 ± 0.069
4.367ValGly: 4.367 ± 0.089
0.868ValHis: 0.868 ± 0.039
6.061ValIle: 6.061 ± 0.102
5.387ValLys: 5.387 ± 0.085
6.056ValLeu: 6.056 ± 0.093
1.736ValMet: 1.736 ± 0.053
3.188ValAsn: 3.188 ± 0.072
2.04ValPro: 2.04 ± 0.059
1.432ValGln: 1.432 ± 0.049
2.237ValArg: 2.237 ± 0.061
3.786ValSer: 3.786 ± 0.075
2.725ValThr: 2.725 ± 0.069
4.391ValVal: 4.391 ± 0.084
0.382ValTrp: 0.382 ± 0.026
1.927ValTyr: 1.927 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.366TrpAla: 0.366 ± 0.023
0.039TrpCys: 0.039 ± 0.008
0.402TrpAsp: 0.402 ± 0.023
0.495TrpGlu: 0.495 ± 0.026
0.293TrpPhe: 0.293 ± 0.021
0.537TrpGly: 0.537 ± 0.028
0.109TrpHis: 0.109 ± 0.012
0.537TrpIle: 0.537 ± 0.026
0.54TrpLys: 0.54 ± 0.027
0.712TrpLeu: 0.712 ± 0.033
0.188TrpMet: 0.188 ± 0.015
0.384TrpAsn: 0.384 ± 0.027
0.268TrpPro: 0.268 ± 0.017
0.331TrpGln: 0.331 ± 0.023
0.31TrpArg: 0.31 ± 0.022
0.409TrpSer: 0.409 ± 0.023
0.324TrpThr: 0.324 ± 0.022
0.343TrpVal: 0.343 ± 0.021
0.092TrpTrp: 0.092 ± 0.012
0.237TrpTyr: 0.237 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.814TyrAla: 1.814 ± 0.054
0.321TyrCys: 0.321 ± 0.021
2.054TyrAsp: 2.054 ± 0.058
2.125TyrGlu: 2.125 ± 0.057
1.972TyrPhe: 1.972 ± 0.057
2.379TyrGly: 2.379 ± 0.06
0.663TyrHis: 0.663 ± 0.032
3.114TyrIle: 3.114 ± 0.07
2.835TyrLys: 2.835 ± 0.079
4.024TyrLeu: 4.024 ± 0.075
0.692TyrMet: 0.692 ± 0.027
2.327TyrAsn: 2.327 ± 0.061
1.38TyrPro: 1.38 ± 0.037
1.466TyrGln: 1.466 ± 0.05
1.705TyrArg: 1.705 ± 0.056
2.519TyrSer: 2.519 ± 0.067
1.6TyrThr: 1.6 ± 0.048
1.633TyrVal: 1.633 ± 0.056
0.31TyrTrp: 0.31 ± 0.025
1.694TyrTyr: 1.694 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2338 proteins (716680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski