Klebsiella phage ST147-VIM1phi7.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Vimunumvirus; Klebsiella virus ST147VIM1phi7-1

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482ME20|A0A482ME20_9CAUD Baseplate assembly protein J OS=Klebsiella phage ST147-VIM1phi7.1 OX=2510480 PE=4 SV=1
MM1 pKa = 7.21QAIAQQGDD9 pKa = 4.15TLDD12 pKa = 4.27MICARR17 pKa = 11.84YY18 pKa = 9.21YY19 pKa = 11.12GRR21 pKa = 11.84TEE23 pKa = 4.05GVFEE27 pKa = 4.48SVLAANPGLAEE38 pKa = 4.58LGAVLPHH45 pKa = 5.77GTVIEE50 pKa = 4.31LPDD53 pKa = 3.74VKK55 pKa = 10.53SSPVTEE61 pKa = 4.96TINLWEE67 pKa = 4.05

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MFM2|A0A482MFM2_9CAUD Uncharacterized protein OS=Klebsiella phage ST147-VIM1phi7.1 OX=2510480 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.18IVIILLALAGAGLLWMRR19 pKa = 11.84HH20 pKa = 5.68DD21 pKa = 3.98NSNLRR26 pKa = 11.84VSFEE30 pKa = 3.76RR31 pKa = 11.84ANRR34 pKa = 11.84VAGTQKK40 pKa = 8.0TTIIMLKK47 pKa = 9.59NQLNVAAEE55 pKa = 3.82QSQRR59 pKa = 11.84IEE61 pKa = 3.98LAQVAMRR68 pKa = 11.84DD69 pKa = 3.48KK70 pKa = 10.68LTTANLLAFRR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.94QTITRR87 pKa = 11.84LLNEE91 pKa = 3.97NEE93 pKa = 3.95AFRR96 pKa = 11.84RR97 pKa = 11.84WYY99 pKa = 10.52RR100 pKa = 11.84ADD102 pKa = 3.85LPDD105 pKa = 3.52AVRR108 pKa = 11.84RR109 pKa = 11.84LHH111 pKa = 6.69QRR113 pKa = 11.84TACTNAAAGDD123 pKa = 3.99CLQRR127 pKa = 11.84LPEE130 pKa = 4.35GQPLPDD136 pKa = 4.13AGQRR140 pKa = 11.84PANN143 pKa = 3.71

Molecular weight: 16.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

10167

33

812

242.1

26.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.875 ± 0.763

0.866 ± 0.121

6.265 ± 0.288

6.236 ± 0.448

3.58 ± 0.282

6.983 ± 0.495

1.613 ± 0.181

5.479 ± 0.214

5.685 ± 0.395

9.088 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.843 ± 0.183

4.229 ± 0.295

4.328 ± 0.287

4.298 ± 0.324

5.882 ± 0.349

5.803 ± 0.259

6.256 ± 0.32

6.58 ± 0.24

1.288 ± 0.146

2.823 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski