Amino acid dipepetide frequency for Arthrobacter phage LittleTokyo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.068AlaAla: 19.068 ± 1.742
1.623AlaCys: 1.623 ± 0.476
7.546AlaAsp: 7.546 ± 0.834
9.25AlaGlu: 9.25 ± 0.977
3.246AlaPhe: 3.246 ± 0.546
15.336AlaGly: 15.336 ± 1.276
1.866AlaHis: 1.866 ± 0.328
3.976AlaIle: 3.976 ± 0.515
2.353AlaLys: 2.353 ± 0.456
10.873AlaLeu: 10.873 ± 1.064
3.083AlaMet: 3.083 ± 0.538
2.759AlaAsn: 2.759 ± 0.472
4.869AlaPro: 4.869 ± 0.586
2.434AlaGln: 2.434 ± 0.439
9.899AlaArg: 9.899 ± 0.95
6.654AlaSer: 6.654 ± 0.823
4.706AlaThr: 4.706 ± 0.561
10.305AlaVal: 10.305 ± 0.72
3.083AlaTrp: 3.083 ± 0.498
2.353AlaTyr: 2.353 ± 0.447
0.0AlaXaa: 0.0 ± 0.0
Cys
0.568CysAla: 0.568 ± 0.22
0.162CysCys: 0.162 ± 0.092
0.73CysAsp: 0.73 ± 0.269
0.73CysGlu: 0.73 ± 0.224
0.081CysPhe: 0.081 ± 0.079
2.678CysGly: 2.678 ± 0.669
0.162CysHis: 0.162 ± 0.132
0.487CysIle: 0.487 ± 0.169
0.243CysLys: 0.243 ± 0.144
0.487CysLeu: 0.487 ± 0.228
0.162CysMet: 0.162 ± 0.118
0.487CysAsn: 0.487 ± 0.188
0.325CysPro: 0.325 ± 0.177
0.406CysGln: 0.406 ± 0.174
1.379CysArg: 1.379 ± 0.372
1.217CysSer: 1.217 ± 0.274
0.487CysThr: 0.487 ± 0.182
0.811CysVal: 0.811 ± 0.3
0.325CysTrp: 0.325 ± 0.172
0.081CysTyr: 0.081 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
5.761AspAla: 5.761 ± 0.631
0.487AspCys: 0.487 ± 0.242
2.84AspAsp: 2.84 ± 0.476
5.355AspGlu: 5.355 ± 0.642
1.866AspPhe: 1.866 ± 0.456
6.248AspGly: 6.248 ± 0.83
0.974AspHis: 0.974 ± 0.294
1.055AspIle: 1.055 ± 0.321
1.866AspLys: 1.866 ± 0.417
6.329AspLeu: 6.329 ± 0.65
1.217AspMet: 1.217 ± 0.33
0.811AspAsn: 0.811 ± 0.24
4.787AspPro: 4.787 ± 0.511
1.298AspGln: 1.298 ± 0.302
3.489AspArg: 3.489 ± 0.531
2.759AspSer: 2.759 ± 0.446
1.704AspThr: 1.704 ± 0.457
5.842AspVal: 5.842 ± 0.73
1.217AspTrp: 1.217 ± 0.277
1.947AspTyr: 1.947 ± 0.342
0.0AspXaa: 0.0 ± 0.0
Glu
6.41GluAla: 6.41 ± 0.766
1.298GluCys: 1.298 ± 0.362
3.489GluAsp: 3.489 ± 0.507
4.463GluGlu: 4.463 ± 0.673
1.785GluPhe: 1.785 ± 0.324
4.219GluGly: 4.219 ± 0.562
1.298GluHis: 1.298 ± 0.388
3.327GluIle: 3.327 ± 0.448
2.272GluLys: 2.272 ± 0.409
6.329GluLeu: 6.329 ± 0.599
1.461GluMet: 1.461 ± 0.412
0.974GluAsn: 0.974 ± 0.283
4.219GluPro: 4.219 ± 0.722
2.434GluGln: 2.434 ± 0.442
4.625GluArg: 4.625 ± 0.735
4.219GluSer: 4.219 ± 0.567
3.327GluThr: 3.327 ± 0.593
6.329GluVal: 6.329 ± 0.714
1.379GluTrp: 1.379 ± 0.374
1.623GluTyr: 1.623 ± 0.254
0.0GluXaa: 0.0 ± 0.0
Phe
3.57PheAla: 3.57 ± 0.53
0.243PheCys: 0.243 ± 0.133
2.11PheAsp: 2.11 ± 0.49
1.866PheGlu: 1.866 ± 0.407
0.487PhePhe: 0.487 ± 0.205
2.029PheGly: 2.029 ± 0.328
0.325PheHis: 0.325 ± 0.157
1.217PheIle: 1.217 ± 0.246
1.217PheLys: 1.217 ± 0.258
1.704PheLeu: 1.704 ± 0.369
0.811PheMet: 0.811 ± 0.254
0.73PheAsn: 0.73 ± 0.251
1.055PhePro: 1.055 ± 0.318
0.406PheGln: 0.406 ± 0.167
2.353PheArg: 2.353 ± 0.455
1.461PheSer: 1.461 ± 0.324
1.623PheThr: 1.623 ± 0.415
1.461PheVal: 1.461 ± 0.319
0.487PheTrp: 0.487 ± 0.189
0.487PheTyr: 0.487 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
11.035GlyAla: 11.035 ± 0.949
1.136GlyCys: 1.136 ± 0.332
5.923GlyAsp: 5.923 ± 0.805
5.599GlyGlu: 5.599 ± 0.744
2.515GlyPhe: 2.515 ± 0.528
10.224GlyGly: 10.224 ± 1.083
0.974GlyHis: 0.974 ± 0.317
3.327GlyIle: 3.327 ± 0.663
3.246GlyLys: 3.246 ± 0.58
9.088GlyLeu: 9.088 ± 1.134
2.434GlyMet: 2.434 ± 0.402
1.379GlyAsn: 1.379 ± 0.372
5.112GlyPro: 5.112 ± 0.642
3.246GlyGln: 3.246 ± 0.56
8.033GlyArg: 8.033 ± 0.798
5.437GlySer: 5.437 ± 0.704
5.031GlyThr: 5.031 ± 0.651
6.573GlyVal: 6.573 ± 0.736
3.246GlyTrp: 3.246 ± 0.476
2.191GlyTyr: 2.191 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
2.11HisAla: 2.11 ± 0.42
0.162HisCys: 0.162 ± 0.105
0.73HisAsp: 0.73 ± 0.254
0.893HisGlu: 0.893 ± 0.291
0.406HisPhe: 0.406 ± 0.163
1.136HisGly: 1.136 ± 0.262
0.243HisHis: 0.243 ± 0.142
0.487HisIle: 0.487 ± 0.218
0.649HisLys: 0.649 ± 0.224
1.542HisLeu: 1.542 ± 0.379
0.243HisMet: 0.243 ± 0.134
0.243HisAsn: 0.243 ± 0.129
1.461HisPro: 1.461 ± 0.389
0.487HisGln: 0.487 ± 0.202
0.974HisArg: 0.974 ± 0.304
0.406HisSer: 0.406 ± 0.191
0.487HisThr: 0.487 ± 0.173
1.217HisVal: 1.217 ± 0.345
0.081HisTrp: 0.081 ± 0.084
0.325HisTyr: 0.325 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
3.976IleAla: 3.976 ± 0.626
0.162IleCys: 0.162 ± 0.108
2.029IleAsp: 2.029 ± 0.337
2.434IleGlu: 2.434 ± 0.511
0.649IlePhe: 0.649 ± 0.234
3.327IleGly: 3.327 ± 0.456
0.325IleHis: 0.325 ± 0.156
0.649IleIle: 0.649 ± 0.22
1.379IleLys: 1.379 ± 0.324
3.327IleLeu: 3.327 ± 0.512
0.325IleMet: 0.325 ± 0.143
1.136IleAsn: 1.136 ± 0.275
1.461IlePro: 1.461 ± 0.35
1.055IleGln: 1.055 ± 0.282
2.678IleArg: 2.678 ± 0.458
2.11IleSer: 2.11 ± 0.466
2.434IleThr: 2.434 ± 0.39
3.165IleVal: 3.165 ± 0.354
0.325IleTrp: 0.325 ± 0.136
0.406IleTyr: 0.406 ± 0.163
0.0IleXaa: 0.0 ± 0.0
Lys
4.138LysAla: 4.138 ± 0.732
0.243LysCys: 0.243 ± 0.135
1.542LysAsp: 1.542 ± 0.369
1.947LysGlu: 1.947 ± 0.339
0.893LysPhe: 0.893 ± 0.254
2.353LysGly: 2.353 ± 0.483
0.811LysHis: 0.811 ± 0.228
1.136LysIle: 1.136 ± 0.323
1.461LysLys: 1.461 ± 0.332
2.84LysLeu: 2.84 ± 0.452
1.217LysMet: 1.217 ± 0.282
1.136LysAsn: 1.136 ± 0.262
2.353LysPro: 2.353 ± 0.63
1.542LysGln: 1.542 ± 0.366
2.921LysArg: 2.921 ± 0.64
2.515LysSer: 2.515 ± 0.399
2.515LysThr: 2.515 ± 0.561
2.678LysVal: 2.678 ± 0.41
0.568LysTrp: 0.568 ± 0.16
0.649LysTyr: 0.649 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
12.009LeuAla: 12.009 ± 0.904
1.217LeuCys: 1.217 ± 0.318
5.193LeuAsp: 5.193 ± 0.722
6.329LeuGlu: 6.329 ± 0.683
2.515LeuPhe: 2.515 ± 0.38
8.52LeuGly: 8.52 ± 0.987
0.811LeuHis: 0.811 ± 0.207
3.165LeuIle: 3.165 ± 0.514
3.408LeuLys: 3.408 ± 0.558
8.358LeuLeu: 8.358 ± 0.98
1.623LeuMet: 1.623 ± 0.315
2.84LeuAsn: 2.84 ± 0.462
6.167LeuPro: 6.167 ± 0.756
2.84LeuGln: 2.84 ± 0.415
6.978LeuArg: 6.978 ± 0.764
3.814LeuSer: 3.814 ± 0.588
4.869LeuThr: 4.869 ± 0.752
6.816LeuVal: 6.816 ± 0.803
1.461LeuTrp: 1.461 ± 0.264
1.217LeuTyr: 1.217 ± 0.288
0.0LeuXaa: 0.0 ± 0.0
Met
2.11MetAla: 2.11 ± 0.4
0.081MetCys: 0.081 ± 0.073
1.542MetAsp: 1.542 ± 0.383
0.893MetGlu: 0.893 ± 0.288
0.325MetPhe: 0.325 ± 0.171
1.947MetGly: 1.947 ± 0.534
0.081MetHis: 0.081 ± 0.086
0.811MetIle: 0.811 ± 0.214
0.893MetLys: 0.893 ± 0.225
1.379MetLeu: 1.379 ± 0.348
0.081MetMet: 0.081 ± 0.065
0.162MetAsn: 0.162 ± 0.129
0.974MetPro: 0.974 ± 0.282
0.243MetGln: 0.243 ± 0.157
2.029MetArg: 2.029 ± 0.348
2.353MetSer: 2.353 ± 0.422
2.11MetThr: 2.11 ± 0.393
1.704MetVal: 1.704 ± 0.325
0.487MetTrp: 0.487 ± 0.21
0.325MetTyr: 0.325 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
1.217AsnAla: 1.217 ± 0.305
0.325AsnCys: 0.325 ± 0.238
1.542AsnAsp: 1.542 ± 0.327
1.136AsnGlu: 1.136 ± 0.281
0.487AsnPhe: 0.487 ± 0.197
2.84AsnGly: 2.84 ± 0.427
0.243AsnHis: 0.243 ± 0.136
0.974AsnIle: 0.974 ± 0.249
1.542AsnLys: 1.542 ± 0.341
2.272AsnLeu: 2.272 ± 0.398
0.243AsnMet: 0.243 ± 0.123
0.649AsnAsn: 0.649 ± 0.282
1.298AsnPro: 1.298 ± 0.274
0.974AsnGln: 0.974 ± 0.215
1.542AsnArg: 1.542 ± 0.342
2.029AsnSer: 2.029 ± 0.37
0.649AsnThr: 0.649 ± 0.191
1.866AsnVal: 1.866 ± 0.464
0.568AsnTrp: 0.568 ± 0.219
0.243AsnTyr: 0.243 ± 0.137
0.0AsnXaa: 0.0 ± 0.0
Pro
11.035ProAla: 11.035 ± 0.956
0.649ProCys: 0.649 ± 0.27
3.327ProAsp: 3.327 ± 0.526
4.219ProGlu: 4.219 ± 0.63
1.055ProPhe: 1.055 ± 0.263
4.95ProGly: 4.95 ± 0.531
0.649ProHis: 0.649 ± 0.217
1.785ProIle: 1.785 ± 0.489
1.461ProLys: 1.461 ± 0.295
4.463ProLeu: 4.463 ± 0.742
1.298ProMet: 1.298 ± 0.294
1.055ProAsn: 1.055 ± 0.24
3.327ProPro: 3.327 ± 0.7
0.649ProGln: 0.649 ± 0.204
3.408ProArg: 3.408 ± 0.498
3.002ProSer: 3.002 ± 0.537
2.597ProThr: 2.597 ± 0.415
6.573ProVal: 6.573 ± 0.76
1.704ProTrp: 1.704 ± 0.326
0.649ProTyr: 0.649 ± 0.233
0.0ProXaa: 0.0 ± 0.0
Gln
4.057GlnAla: 4.057 ± 0.516
0.162GlnCys: 0.162 ± 0.11
1.461GlnAsp: 1.461 ± 0.332
1.785GlnGlu: 1.785 ± 0.378
0.649GlnPhe: 0.649 ± 0.203
2.353GlnGly: 2.353 ± 0.406
0.974GlnHis: 0.974 ± 0.293
1.136GlnIle: 1.136 ± 0.268
1.055GlnLys: 1.055 ± 0.299
3.733GlnLeu: 3.733 ± 0.665
0.406GlnMet: 0.406 ± 0.163
0.811GlnAsn: 0.811 ± 0.247
2.191GlnPro: 2.191 ± 0.442
0.811GlnGln: 0.811 ± 0.302
1.542GlnArg: 1.542 ± 0.402
0.649GlnSer: 0.649 ± 0.251
1.623GlnThr: 1.623 ± 0.413
2.434GlnVal: 2.434 ± 0.48
0.649GlnTrp: 0.649 ± 0.231
0.325GlnTyr: 0.325 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
9.656ArgAla: 9.656 ± 0.855
1.298ArgCys: 1.298 ± 0.324
4.787ArgAsp: 4.787 ± 0.579
4.787ArgGlu: 4.787 ± 0.699
2.11ArgPhe: 2.11 ± 0.421
5.842ArgGly: 5.842 ± 0.606
0.811ArgHis: 0.811 ± 0.321
2.597ArgIle: 2.597 ± 0.463
2.759ArgLys: 2.759 ± 0.411
5.923ArgLeu: 5.923 ± 0.734
1.461ArgMet: 1.461 ± 0.333
1.947ArgAsn: 1.947 ± 0.351
4.544ArgPro: 4.544 ± 0.581
3.165ArgGln: 3.165 ± 0.511
6.248ArgArg: 6.248 ± 1.009
4.301ArgSer: 4.301 ± 0.551
4.787ArgThr: 4.787 ± 0.694
6.248ArgVal: 6.248 ± 0.645
1.542ArgTrp: 1.542 ± 0.388
1.866ArgTyr: 1.866 ± 0.472
0.0ArgXaa: 0.0 ± 0.0
Ser
6.978SerAla: 6.978 ± 0.675
0.568SerCys: 0.568 ± 0.217
2.434SerAsp: 2.434 ± 0.549
2.353SerGlu: 2.353 ± 0.463
1.866SerPhe: 1.866 ± 0.41
6.897SerGly: 6.897 ± 0.78
0.73SerHis: 0.73 ± 0.23
1.947SerIle: 1.947 ± 0.41
2.272SerLys: 2.272 ± 0.476
4.95SerLeu: 4.95 ± 0.565
1.542SerMet: 1.542 ± 0.359
1.136SerAsn: 1.136 ± 0.28
2.434SerPro: 2.434 ± 0.395
1.217SerGln: 1.217 ± 0.333
4.625SerArg: 4.625 ± 0.711
3.489SerSer: 3.489 ± 0.508
2.597SerThr: 2.597 ± 0.446
4.869SerVal: 4.869 ± 0.608
2.191SerTrp: 2.191 ± 0.377
1.055SerTyr: 1.055 ± 0.358
0.0SerXaa: 0.0 ± 0.0
Thr
6.978ThrAla: 6.978 ± 0.751
0.325ThrCys: 0.325 ± 0.161
2.597ThrAsp: 2.597 ± 0.51
2.515ThrGlu: 2.515 ± 0.491
1.217ThrPhe: 1.217 ± 0.336
5.68ThrGly: 5.68 ± 0.563
0.73ThrHis: 0.73 ± 0.245
2.191ThrIle: 2.191 ± 0.471
1.866ThrLys: 1.866 ± 0.425
3.895ThrLeu: 3.895 ± 0.512
0.568ThrMet: 0.568 ± 0.2
1.785ThrAsn: 1.785 ± 0.351
3.002ThrPro: 3.002 ± 0.468
1.055ThrGln: 1.055 ± 0.288
3.083ThrArg: 3.083 ± 0.663
2.759ThrSer: 2.759 ± 0.497
3.651ThrThr: 3.651 ± 0.574
6.248ThrVal: 6.248 ± 0.825
1.136ThrTrp: 1.136 ± 0.325
1.461ThrTyr: 1.461 ± 0.488
0.0ThrXaa: 0.0 ± 0.0
Val
10.143ValAla: 10.143 ± 0.801
0.974ValCys: 0.974 ± 0.449
4.869ValAsp: 4.869 ± 0.555
6.248ValGlu: 6.248 ± 0.652
2.191ValPhe: 2.191 ± 0.396
5.842ValGly: 5.842 ± 0.861
1.623ValHis: 1.623 ± 0.334
2.353ValIle: 2.353 ± 0.483
4.057ValLys: 4.057 ± 0.579
8.114ValLeu: 8.114 ± 1.027
1.217ValMet: 1.217 ± 0.28
1.785ValAsn: 1.785 ± 0.372
5.68ValPro: 5.68 ± 0.736
2.353ValGln: 2.353 ± 0.451
6.978ValArg: 6.978 ± 0.823
4.382ValSer: 4.382 ± 0.462
5.193ValThr: 5.193 ± 0.921
7.303ValVal: 7.303 ± 0.823
2.272ValTrp: 2.272 ± 0.326
1.947ValTyr: 1.947 ± 0.356
0.0ValXaa: 0.0 ± 0.0
Trp
2.11TrpAla: 2.11 ± 0.376
0.243TrpCys: 0.243 ± 0.143
1.866TrpAsp: 1.866 ± 0.397
1.136TrpGlu: 1.136 ± 0.298
0.568TrpPhe: 0.568 ± 0.232
1.461TrpGly: 1.461 ± 0.312
0.568TrpHis: 0.568 ± 0.225
0.487TrpIle: 0.487 ± 0.31
0.649TrpLys: 0.649 ± 0.265
2.191TrpLeu: 2.191 ± 0.41
0.325TrpMet: 0.325 ± 0.161
0.487TrpAsn: 0.487 ± 0.196
1.704TrpPro: 1.704 ± 0.313
1.461TrpGln: 1.461 ± 0.35
2.191TrpArg: 2.191 ± 0.474
1.379TrpSer: 1.379 ± 0.306
1.623TrpThr: 1.623 ± 0.321
2.029TrpVal: 2.029 ± 0.392
0.73TrpTrp: 0.73 ± 0.2
0.568TrpTyr: 0.568 ± 0.255
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.353TyrAla: 2.353 ± 0.563
0.649TyrCys: 0.649 ± 0.258
1.298TyrAsp: 1.298 ± 0.297
1.542TyrGlu: 1.542 ± 0.3
0.73TyrPhe: 0.73 ± 0.227
1.866TyrGly: 1.866 ± 0.41
0.081TyrHis: 0.081 ± 0.081
0.243TyrIle: 0.243 ± 0.14
0.811TyrLys: 0.811 ± 0.25
2.353TyrLeu: 2.353 ± 0.627
0.73TyrMet: 0.73 ± 0.273
0.325TyrAsn: 0.325 ± 0.15
0.893TyrPro: 0.893 ± 0.284
0.73TyrGln: 0.73 ± 0.221
1.785TyrArg: 1.785 ± 0.422
1.217TyrSer: 1.217 ± 0.277
0.73TyrThr: 0.73 ± 0.249
1.055TyrVal: 1.055 ± 0.278
0.325TyrTrp: 0.325 ± 0.156
0.325TyrTyr: 0.325 ± 0.162
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (12325 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski