Gopherus agassizii (Agassiz s desert tortoise)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota;

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31812 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A452GN57|A0A452GN57_9SAUR Olfactory receptor OS=Gopherus agassizii OX=38772 PE=3 SV=1
MM1 pKa = 7.6PVLPFPASLHH11 pKa = 5.25GAEE14 pKa = 4.87PLFHH18 pKa = 7.46DD19 pKa = 5.39LDD21 pKa = 5.41SDD23 pKa = 3.99DD24 pKa = 4.49TSLSNLGDD32 pKa = 3.78CFLATSEE39 pKa = 4.47AGPLQSRR46 pKa = 11.84MGNPIDD52 pKa = 4.58QLYY55 pKa = 11.25SMQNSYY61 pKa = 8.25FTSS64 pKa = 3.4

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A452IAC7|A0A452IAC7_9SAUR Chondromodulin OS=Gopherus agassizii OX=38772 PE=3 SV=1
SS1 pKa = 6.64PAGNLGAAITPLSGTGLRR19 pKa = 11.84SPVPPRR25 pKa = 11.84AGTGLRR31 pKa = 11.84SPVPPRR37 pKa = 11.84AGTGLRR43 pKa = 11.84SPVPPRR49 pKa = 11.84AGTGLRR55 pKa = 11.84SPVPPRR61 pKa = 11.84AGTGLRR67 pKa = 11.84SPVPPRR73 pKa = 11.84AGTGLWSPIPPRR85 pKa = 11.84AGTGLRR91 pKa = 11.84SPVPPRR97 pKa = 11.84VAGTGLRR104 pKa = 11.84WPVPPRR110 pKa = 11.84AGTGLRR116 pKa = 11.84SPVPPRR122 pKa = 11.84AGTGLRR128 pKa = 11.84SPVPPRR134 pKa = 11.84AGTGLRR140 pKa = 11.84SPVPPRR146 pKa = 11.84AGTGLRR152 pKa = 11.84SPVPPRR158 pKa = 11.84AGTGLRR164 pKa = 11.84SPVPPRR170 pKa = 11.84AGTGLRR176 pKa = 11.84SPVPPRR182 pKa = 11.84AGTGLRR188 pKa = 11.84SPVPPRR194 pKa = 11.84AGTGLRR200 pKa = 11.84SPVPPRR206 pKa = 11.84AGTGLRR212 pKa = 11.84SPVPPRR218 pKa = 11.84AGTGLRR224 pKa = 11.84SPVPPRR230 pKa = 11.84AGTGLRR236 pKa = 11.84SPVPPRR242 pKa = 11.84AGTGLRR248 pKa = 11.84SPVPPRR254 pKa = 11.84AGTGLRR260 pKa = 11.84WPVPPRR266 pKa = 11.84AGTGLRR272 pKa = 11.84SPVPPRR278 pKa = 11.84AGPGSWTHH286 pKa = 6.3GPLPQPTGGPQRR298 pKa = 11.84GHH300 pKa = 7.13APACPPPPAAPWEE313 pKa = 4.15LGRR316 pKa = 11.84CSLTPGAGPSPLPPAARR333 pKa = 3.88

Molecular weight: 33.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20096

11716

31812

19797352

18

6780

622.3

69.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.515 ± 0.012

2.281 ± 0.011

4.882 ± 0.009

7.235 ± 0.018

3.689 ± 0.009

6.062 ± 0.017

2.588 ± 0.006

4.856 ± 0.011

6.132 ± 0.017

9.789 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.006

4.018 ± 0.01

5.558 ± 0.017

4.849 ± 0.011

5.343 ± 0.011

8.431 ± 0.016

5.428 ± 0.009

6.027 ± 0.011

1.195 ± 0.005

2.828 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski