Amino acid dipepetide frequency for Rummeliibacillus stabekisii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.71AlaAla: 5.71 ± 0.081
0.59AlaCys: 0.59 ± 0.025
3.443AlaAsp: 3.443 ± 0.061
4.746AlaGlu: 4.746 ± 0.07
3.238AlaPhe: 3.238 ± 0.063
5.106AlaGly: 5.106 ± 0.09
1.352AlaHis: 1.352 ± 0.042
6.565AlaIle: 6.565 ± 0.111
5.426AlaLys: 5.426 ± 0.089
7.212AlaLeu: 7.212 ± 0.102
2.118AlaMet: 2.118 ± 0.053
3.064AlaAsn: 3.064 ± 0.058
2.034AlaPro: 2.034 ± 0.053
2.431AlaGln: 2.431 ± 0.053
2.569AlaArg: 2.569 ± 0.057
4.167AlaSer: 4.167 ± 0.078
3.927AlaThr: 3.927 ± 0.071
5.084AlaVal: 5.084 ± 0.085
0.579AlaTrp: 0.579 ± 0.026
2.365AlaTyr: 2.365 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.432CysAla: 0.432 ± 0.022
0.084CysCys: 0.084 ± 0.01
0.35CysAsp: 0.35 ± 0.019
0.452CysGlu: 0.452 ± 0.024
0.339CysPhe: 0.339 ± 0.02
0.643CysGly: 0.643 ± 0.027
0.189CysHis: 0.189 ± 0.015
0.58CysIle: 0.58 ± 0.028
0.354CysLys: 0.354 ± 0.021
0.659CysLeu: 0.659 ± 0.031
0.177CysMet: 0.177 ± 0.014
0.279CysAsn: 0.279 ± 0.016
0.35CysPro: 0.35 ± 0.019
0.283CysGln: 0.283 ± 0.016
0.238CysArg: 0.238 ± 0.018
0.44CysSer: 0.44 ± 0.02
0.403CysThr: 0.403 ± 0.021
0.376CysVal: 0.376 ± 0.02
0.066CysTrp: 0.066 ± 0.008
0.25CysTyr: 0.25 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.415AspAla: 3.415 ± 0.059
0.405AspCys: 0.405 ± 0.022
2.493AspAsp: 2.493 ± 0.053
4.589AspGlu: 4.589 ± 0.085
2.537AspPhe: 2.537 ± 0.054
3.357AspGly: 3.357 ± 0.075
1.079AspHis: 1.079 ± 0.033
4.204AspIle: 4.204 ± 0.079
3.161AspLys: 3.161 ± 0.067
5.152AspLeu: 5.152 ± 0.081
1.349AspMet: 1.349 ± 0.037
1.859AspAsn: 1.859 ± 0.053
1.766AspPro: 1.766 ± 0.046
2.084AspGln: 2.084 ± 0.043
2.023AspArg: 2.023 ± 0.055
2.647AspSer: 2.647 ± 0.053
2.64AspThr: 2.64 ± 0.055
3.601AspVal: 3.601 ± 0.068
0.577AspTrp: 0.577 ± 0.028
2.23AspTyr: 2.23 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
5.45GluAla: 5.45 ± 0.093
0.417GluCys: 0.417 ± 0.022
3.884GluAsp: 3.884 ± 0.074
7.13GluGlu: 7.13 ± 0.113
2.564GluPhe: 2.564 ± 0.047
4.251GluGly: 4.251 ± 0.084
1.581GluHis: 1.581 ± 0.041
5.737GluIle: 5.737 ± 0.091
6.567GluLys: 6.567 ± 0.103
7.061GluLeu: 7.061 ± 0.102
2.318GluMet: 2.318 ± 0.058
3.61GluAsn: 3.61 ± 0.071
1.84GluPro: 1.84 ± 0.046
3.354GluGln: 3.354 ± 0.076
3.35GluArg: 3.35 ± 0.072
3.553GluSer: 3.553 ± 0.069
3.78GluThr: 3.78 ± 0.064
4.979GluVal: 4.979 ± 0.077
0.827GluTrp: 0.827 ± 0.031
2.262GluTyr: 2.262 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.085PheAla: 3.085 ± 0.059
0.336PheCys: 0.336 ± 0.022
2.388PheAsp: 2.388 ± 0.051
2.855PheGlu: 2.855 ± 0.062
2.244PhePhe: 2.244 ± 0.067
3.207PheGly: 3.207 ± 0.075
0.981PheHis: 0.981 ± 0.029
3.904PheIle: 3.904 ± 0.077
2.734PheLys: 2.734 ± 0.06
4.286PheLeu: 4.286 ± 0.086
1.185PheMet: 1.185 ± 0.04
1.974PheAsn: 1.974 ± 0.046
1.443PhePro: 1.443 ± 0.036
1.489PheGln: 1.489 ± 0.043
1.442PheArg: 1.442 ± 0.043
3.056PheSer: 3.056 ± 0.07
2.556PheThr: 2.556 ± 0.051
2.971PheVal: 2.971 ± 0.068
0.427PheTrp: 0.427 ± 0.022
1.744PheTyr: 1.744 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
4.624GlyAla: 4.624 ± 0.096
0.543GlyCys: 0.543 ± 0.024
3.196GlyAsp: 3.196 ± 0.063
4.324GlyGlu: 4.324 ± 0.067
3.317GlyPhe: 3.317 ± 0.07
4.576GlyGly: 4.576 ± 0.103
1.424GlyHis: 1.424 ± 0.042
5.859GlyIle: 5.859 ± 0.082
5.187GlyLys: 5.187 ± 0.094
6.29GlyLeu: 6.29 ± 0.101
1.983GlyMet: 1.983 ± 0.051
2.696GlyAsn: 2.696 ± 0.064
1.548GlyPro: 1.548 ± 0.044
2.204GlyGln: 2.204 ± 0.053
2.506GlyArg: 2.506 ± 0.058
3.815GlySer: 3.815 ± 0.066
3.871GlyThr: 3.871 ± 0.077
4.794GlyVal: 4.794 ± 0.083
0.719GlyTrp: 0.719 ± 0.031
2.808GlyTyr: 2.808 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
1.377HisAla: 1.377 ± 0.043
0.175HisCys: 0.175 ± 0.013
1.018HisAsp: 1.018 ± 0.032
1.389HisGlu: 1.389 ± 0.039
1.075HisPhe: 1.075 ± 0.037
1.344HisGly: 1.344 ± 0.041
0.631HisHis: 0.631 ± 0.029
1.648HisIle: 1.648 ± 0.041
1.167HisLys: 1.167 ± 0.034
2.188HisLeu: 2.188 ± 0.052
0.559HisMet: 0.559 ± 0.025
0.772HisAsn: 0.772 ± 0.027
1.071HisPro: 1.071 ± 0.039
0.816HisGln: 0.816 ± 0.031
0.804HisArg: 0.804 ± 0.028
1.203HisSer: 1.203 ± 0.034
1.151HisThr: 1.151 ± 0.039
1.422HisVal: 1.422 ± 0.039
0.181HisTrp: 0.181 ± 0.015
0.867HisTyr: 0.867 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.582IleAla: 6.582 ± 0.097
0.647IleCys: 0.647 ± 0.028
4.449IleAsp: 4.449 ± 0.076
5.943IleGlu: 5.943 ± 0.086
3.342IlePhe: 3.342 ± 0.077
5.896IleGly: 5.896 ± 0.103
1.648IleHis: 1.648 ± 0.044
5.998IleIle: 5.998 ± 0.096
4.838IleLys: 4.838 ± 0.077
7.281IleLeu: 7.281 ± 0.114
1.906IleMet: 1.906 ± 0.045
3.441IleAsn: 3.441 ± 0.059
3.328IlePro: 3.328 ± 0.068
3.2IleGln: 3.2 ± 0.065
3.129IleArg: 3.129 ± 0.064
5.15IleSer: 5.15 ± 0.079
4.323IleThr: 4.323 ± 0.076
5.781IleVal: 5.781 ± 0.092
0.659IleTrp: 0.659 ± 0.029
2.519IleTyr: 2.519 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
5.095LysAla: 5.095 ± 0.073
0.354LysCys: 0.354 ± 0.021
4.163LysAsp: 4.163 ± 0.089
7.308LysGlu: 7.308 ± 0.091
2.132LysPhe: 2.132 ± 0.057
4.69LysGly: 4.69 ± 0.079
1.34LysHis: 1.34 ± 0.039
4.804LysIle: 4.804 ± 0.083
6.75LysLys: 6.75 ± 0.116
6.061LysLeu: 6.061 ± 0.089
2.377LysMet: 2.377 ± 0.053
3.722LysAsn: 3.722 ± 0.068
2.421LysPro: 2.421 ± 0.061
3.134LysGln: 3.134 ± 0.08
3.247LysArg: 3.247 ± 0.059
3.804LysSer: 3.804 ± 0.072
4.202LysThr: 4.202 ± 0.076
4.953LysVal: 4.953 ± 0.09
0.905LysTrp: 0.905 ± 0.034
2.387LysTyr: 2.387 ± 0.053
0.0LysXaa: 0.0 ± 0.0
Leu
7.307LeuAla: 7.307 ± 0.113
0.712LeuCys: 0.712 ± 0.03
4.767LeuAsp: 4.767 ± 0.068
6.498LeuGlu: 6.498 ± 0.101
4.615LeuPhe: 4.615 ± 0.098
6.032LeuGly: 6.032 ± 0.097
1.95LeuHis: 1.95 ± 0.052
7.35LeuIle: 7.35 ± 0.118
6.964LeuLys: 6.964 ± 0.102
9.937LeuLeu: 9.937 ± 0.152
2.474LeuMet: 2.474 ± 0.05
4.37LeuAsn: 4.37 ± 0.075
3.83LeuPro: 3.83 ± 0.061
3.8LeuGln: 3.8 ± 0.072
3.518LeuArg: 3.518 ± 0.07
6.784LeuSer: 6.784 ± 0.102
5.482LeuThr: 5.482 ± 0.072
5.995LeuVal: 5.995 ± 0.093
0.78LeuTrp: 0.78 ± 0.03
3.18LeuTyr: 3.18 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.206MetAla: 2.206 ± 0.05
0.137MetCys: 0.137 ± 0.013
1.501MetAsp: 1.501 ± 0.043
1.896MetGlu: 1.896 ± 0.047
0.982MetPhe: 0.982 ± 0.032
1.784MetGly: 1.784 ± 0.047
0.531MetHis: 0.531 ± 0.026
2.106MetIle: 2.106 ± 0.049
2.519MetLys: 2.519 ± 0.06
2.524MetLeu: 2.524 ± 0.053
0.871MetMet: 0.871 ± 0.035
1.605MetAsn: 1.605 ± 0.038
1.171MetPro: 1.171 ± 0.044
1.045MetGln: 1.045 ± 0.034
1.126MetArg: 1.126 ± 0.036
1.577MetSer: 1.577 ± 0.044
1.664MetThr: 1.664 ± 0.044
1.681MetVal: 1.681 ± 0.045
0.198MetTrp: 0.198 ± 0.016
0.791MetTyr: 0.791 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.029AsnAla: 3.029 ± 0.063
0.277AsnCys: 0.277 ± 0.017
2.349AsnAsp: 2.349 ± 0.055
3.478AsnGlu: 3.478 ± 0.061
1.676AsnPhe: 1.676 ± 0.047
3.337AsnGly: 3.337 ± 0.071
0.976AsnHis: 0.976 ± 0.034
3.399AsnIle: 3.399 ± 0.063
3.333AsnLys: 3.333 ± 0.073
3.869AsnLeu: 3.869 ± 0.078
1.185AsnMet: 1.185 ± 0.035
2.301AsnAsn: 2.301 ± 0.057
1.978AsnPro: 1.978 ± 0.049
1.9AsnGln: 1.9 ± 0.052
2.005AsnArg: 2.005 ± 0.043
2.538AsnSer: 2.538 ± 0.068
2.481AsnThr: 2.481 ± 0.055
2.956AsnVal: 2.956 ± 0.059
0.542AsnTrp: 0.542 ± 0.023
1.632AsnTyr: 1.632 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.189ProAla: 2.189 ± 0.051
0.219ProCys: 0.219 ± 0.015
1.861ProAsp: 1.861 ± 0.055
2.647ProGlu: 2.647 ± 0.062
1.915ProPhe: 1.915 ± 0.054
2.014ProGly: 2.014 ± 0.048
0.777ProHis: 0.777 ± 0.028
2.906ProIle: 2.906 ± 0.062
2.376ProLys: 2.376 ± 0.057
3.315ProLeu: 3.315 ± 0.063
0.895ProMet: 0.895 ± 0.032
1.67ProAsn: 1.67 ± 0.041
0.904ProPro: 0.904 ± 0.029
1.167ProGln: 1.167 ± 0.034
1.028ProArg: 1.028 ± 0.032
2.291ProSer: 2.291 ± 0.048
2.037ProThr: 2.037 ± 0.047
2.686ProVal: 2.686 ± 0.063
0.331ProTrp: 0.331 ± 0.021
1.468ProTyr: 1.468 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
2.764GlnAla: 2.764 ± 0.055
0.202GlnCys: 0.202 ± 0.016
1.786GlnAsp: 1.786 ± 0.045
2.946GlnGlu: 2.946 ± 0.062
1.601GlnPhe: 1.601 ± 0.042
2.068GlnGly: 2.068 ± 0.048
0.855GlnHis: 0.855 ± 0.033
2.763GlnIle: 2.763 ± 0.065
2.988GlnLys: 2.988 ± 0.067
4.267GlnLeu: 4.267 ± 0.088
1.167GlnMet: 1.167 ± 0.036
1.691GlnAsn: 1.691 ± 0.046
1.277GlnPro: 1.277 ± 0.037
2.085GlnGln: 2.085 ± 0.073
1.486GlnArg: 1.486 ± 0.04
2.277GlnSer: 2.277 ± 0.055
2.092GlnThr: 2.092 ± 0.05
2.26GlnVal: 2.26 ± 0.047
0.395GlnTrp: 0.395 ± 0.022
1.514GlnTyr: 1.514 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
2.44ArgAla: 2.44 ± 0.06
0.219ArgCys: 0.219 ± 0.018
2.052ArgAsp: 2.052 ± 0.052
3.024ArgGlu: 3.024 ± 0.063
1.83ArgPhe: 1.83 ± 0.038
2.199ArgGly: 2.199 ± 0.053
0.823ArgHis: 0.823 ± 0.027
3.098ArgIle: 3.098 ± 0.06
3.216ArgLys: 3.216 ± 0.061
3.823ArgLeu: 3.823 ± 0.069
1.245ArgMet: 1.245 ± 0.038
1.828ArgAsn: 1.828 ± 0.043
1.257ArgPro: 1.257 ± 0.042
1.517ArgGln: 1.517 ± 0.044
1.683ArgArg: 1.683 ± 0.046
2.018ArgSer: 2.018 ± 0.041
2.024ArgThr: 2.024 ± 0.045
2.497ArgVal: 2.497 ± 0.047
0.329ArgTrp: 0.329 ± 0.016
1.499ArgTyr: 1.499 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
3.887SerAla: 3.887 ± 0.069
0.371SerCys: 0.371 ± 0.025
2.697SerAsp: 2.697 ± 0.057
3.751SerGlu: 3.751 ± 0.07
3.124SerPhe: 3.124 ± 0.065
4.12SerGly: 4.12 ± 0.078
1.234SerHis: 1.234 ± 0.043
5.411SerIle: 5.411 ± 0.071
4.349SerLys: 4.349 ± 0.071
5.984SerLeu: 5.984 ± 0.102
1.652SerMet: 1.652 ± 0.043
2.791SerAsn: 2.791 ± 0.06
2.033SerPro: 2.033 ± 0.042
2.047SerGln: 2.047 ± 0.048
2.156SerArg: 2.156 ± 0.053
3.997SerSer: 3.997 ± 0.077
3.426SerThr: 3.426 ± 0.07
3.976SerVal: 3.976 ± 0.078
0.553SerTrp: 0.553 ± 0.025
2.302SerTyr: 2.302 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
4.185ThrAla: 4.185 ± 0.072
0.323ThrCys: 0.323 ± 0.019
2.746ThrAsp: 2.746 ± 0.064
3.627ThrGlu: 3.627 ± 0.066
2.588ThrPhe: 2.588 ± 0.052
4.103ThrGly: 4.103 ± 0.077
1.088ThrHis: 1.088 ± 0.035
4.863ThrIle: 4.863 ± 0.073
3.88ThrLys: 3.88 ± 0.076
5.254ThrLeu: 5.254 ± 0.082
1.393ThrMet: 1.393 ± 0.041
2.589ThrAsn: 2.589 ± 0.063
2.284ThrPro: 2.284 ± 0.049
1.635ThrGln: 1.635 ± 0.039
1.793ThrArg: 1.793 ± 0.05
3.373ThrSer: 3.373 ± 0.058
3.316ThrThr: 3.316 ± 0.064
4.245ThrVal: 4.245 ± 0.076
0.488ThrTrp: 0.488 ± 0.023
1.963ThrTyr: 1.963 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
5.091ValAla: 5.091 ± 0.084
0.579ValCys: 0.579 ± 0.025
3.589ValAsp: 3.589 ± 0.073
4.91ValGlu: 4.91 ± 0.081
2.896ValPhe: 2.896 ± 0.054
4.577ValGly: 4.577 ± 0.083
1.311ValHis: 1.311 ± 0.042
5.351ValIle: 5.351 ± 0.086
4.857ValLys: 4.857 ± 0.079
6.509ValLeu: 6.509 ± 0.091
1.827ValMet: 1.827 ± 0.045
2.976ValAsn: 2.976 ± 0.056
2.61ValPro: 2.61 ± 0.065
2.363ValGln: 2.363 ± 0.051
2.558ValArg: 2.558 ± 0.052
4.403ValSer: 4.403 ± 0.073
3.853ValThr: 3.853 ± 0.077
4.857ValVal: 4.857 ± 0.091
0.569ValTrp: 0.569 ± 0.027
2.244ValTyr: 2.244 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.552TrpAla: 0.552 ± 0.028
0.067TrpCys: 0.067 ± 0.008
0.466TrpAsp: 0.466 ± 0.025
0.526TrpGlu: 0.526 ± 0.026
0.483TrpPhe: 0.483 ± 0.023
0.621TrpGly: 0.621 ± 0.028
0.232TrpHis: 0.232 ± 0.017
0.873TrpIle: 0.873 ± 0.036
0.76TrpLys: 0.76 ± 0.027
1.153TrpLeu: 1.153 ± 0.04
0.325TrpMet: 0.325 ± 0.019
0.464TrpAsn: 0.464 ± 0.023
0.25TrpPro: 0.25 ± 0.017
0.409TrpGln: 0.409 ± 0.024
0.408TrpArg: 0.408 ± 0.024
0.575TrpSer: 0.575 ± 0.025
0.501TrpThr: 0.501 ± 0.024
0.546TrpVal: 0.546 ± 0.023
0.119TrpTrp: 0.119 ± 0.011
0.327TrpTyr: 0.327 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.303TyrAla: 2.303 ± 0.051
0.287TyrCys: 0.287 ± 0.018
1.97TyrAsp: 1.97 ± 0.051
2.476TyrGlu: 2.476 ± 0.059
1.808TyrPhe: 1.808 ± 0.044
2.36TyrGly: 2.36 ± 0.055
0.855TyrHis: 0.855 ± 0.03
2.643TyrIle: 2.643 ± 0.059
2.426TyrLys: 2.426 ± 0.055
3.521TyrLeu: 3.521 ± 0.065
0.908TyrMet: 0.908 ± 0.031
1.538TyrAsn: 1.538 ± 0.045
1.303TyrPro: 1.303 ± 0.042
1.522TyrGln: 1.522 ± 0.041
1.553TyrArg: 1.553 ± 0.039
2.191TyrSer: 2.191 ± 0.048
2.009TyrThr: 2.009 ± 0.047
2.268TyrVal: 2.268 ± 0.054
0.411TyrTrp: 0.411 ± 0.024
1.457TyrTyr: 1.457 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3235 proteins (927364 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski