Lewinella agarilytica

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Lewinellaceae; Lewinella

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4889 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9J3Z8|A0A1H9J3Z8_9BACT Uncharacterized protein OS=Lewinella agarilytica OX=478744 GN=SAMN05444359_11678 PE=4 SV=1
MM1 pKa = 7.41LKK3 pKa = 10.39KK4 pKa = 10.77LSFLLAACLIVMTGCKK20 pKa = 9.71DD21 pKa = 3.54DD22 pKa = 4.09EE23 pKa = 4.47LAPVIIFNEE32 pKa = 4.29LEE34 pKa = 3.89VGAFPFLNEE43 pKa = 4.11LKK45 pKa = 10.12TGEE48 pKa = 4.43FDD50 pKa = 4.4LADD53 pKa = 4.05LAGSAYY59 pKa = 10.15EE60 pKa = 3.79MDD62 pKa = 4.08VYY64 pKa = 10.93FVDD67 pKa = 4.6GQAGANIAQYY77 pKa = 10.42NVYY80 pKa = 8.66VTFEE84 pKa = 4.93DD85 pKa = 3.97NNPSNGDD92 pKa = 3.34EE93 pKa = 4.28SSTAEE98 pKa = 3.92ALFRR102 pKa = 11.84SYY104 pKa = 11.21VPSDD108 pKa = 3.5FEE110 pKa = 4.75TIGDD114 pKa = 3.98KK115 pKa = 11.46GNLGTNVVIPFLDD128 pKa = 3.53AATAAGVDD136 pKa = 3.55ADD138 pKa = 4.28NVLSGDD144 pKa = 3.41RR145 pKa = 11.84FQFRR149 pKa = 11.84TEE151 pKa = 3.62IVTTTGTTFNSVNSTPAVTNAFGGLWNFNVTATCPLADD189 pKa = 3.76DD190 pKa = 4.5VFVGSYY196 pKa = 9.35NVSYY200 pKa = 10.67GYY202 pKa = 10.19IYY204 pKa = 10.65DD205 pKa = 3.63PFVIFGRR212 pKa = 11.84TIQGWLPAPMDD223 pKa = 3.58QNVTLEE229 pKa = 4.3LKK231 pKa = 10.69AGSTTVRR238 pKa = 11.84SFGTQYY244 pKa = 11.16LVPSDD249 pKa = 4.03LTSDD253 pKa = 3.14ITIDD257 pKa = 3.47LTFSCDD263 pKa = 2.92VVTAVSSDD271 pKa = 3.17SGVRR275 pKa = 11.84CSGAGWRR282 pKa = 11.84PIQINTASFDD292 pKa = 4.32LNDD295 pKa = 4.57DD296 pKa = 3.62STFTIEE302 pKa = 4.05FRR304 pKa = 11.84DD305 pKa = 3.48WGSDD309 pKa = 3.5EE310 pKa = 5.22NNGGCGNIAAQEE322 pKa = 4.05YY323 pKa = 9.9SVVFVKK329 pKa = 10.35QQ330 pKa = 3.04

Molecular weight: 35.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9KXV2|A0A1H9KXV2_9BACT Lipoprotein-releasing system permease protein OS=Lewinella agarilytica OX=478744 GN=SAMN05444359_12234 PE=4 SV=1
MM1 pKa = 7.22GRR3 pKa = 11.84SPQVQGGLKK12 pKa = 9.26GQWGSLSLLPLCCSTVACTCVSAATFFWSNFSRR45 pKa = 11.84TCTSSPAGVKK55 pKa = 10.23RR56 pKa = 11.84ILVQYY61 pKa = 10.1NISLHH66 pKa = 6.33LFVLRR71 pKa = 11.84PSAYY75 pKa = 9.57FSAGFSFVEE84 pKa = 4.23ARR86 pKa = 11.84SKK88 pKa = 10.35FARR91 pKa = 11.84ARR93 pKa = 11.84AIWGLFVPEE102 pKa = 4.04QVPSEE107 pKa = 4.05RR108 pKa = 11.84SEE110 pKa = 3.67PRR112 pKa = 11.84YY113 pKa = 8.65TFDD116 pKa = 3.93KK117 pKa = 10.87LSGVAPRR124 pKa = 11.84GGVKK128 pKa = 9.95KK129 pKa = 10.54QEE131 pKa = 4.03FMLHH135 pKa = 5.62CTNSSHH141 pKa = 6.64HH142 pKa = 6.91KK143 pKa = 9.8SRR145 pKa = 11.84PVLPLLLFRR154 pKa = 11.84PEE156 pKa = 4.0RR157 pKa = 11.84NSVRR161 pKa = 11.84EE162 pKa = 4.18TCCLHH167 pKa = 6.23PRR169 pKa = 11.84RR170 pKa = 11.84GRR172 pKa = 11.84KK173 pKa = 9.3AISRR177 pKa = 3.94

Molecular weight: 19.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4889

0

4889

1822202

26

5781

372.7

41.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.404 ± 0.037

0.933 ± 0.023

6.117 ± 0.043

6.402 ± 0.033

4.657 ± 0.027

7.87 ± 0.043

1.79 ± 0.019

5.66 ± 0.025

4.387 ± 0.048

10.003 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.159 ± 0.018

4.399 ± 0.029

4.579 ± 0.028

3.535 ± 0.021

5.482 ± 0.033

6.187 ± 0.031

6.036 ± 0.05

6.546 ± 0.024

1.284 ± 0.016

3.568 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski