Imperialibacter sp. EC-SDR9

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Flammeovirgaceae; Imperialibacter; unclassified Imperialibacter

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E7Z2U0|A0A5E7Z2U0_9BACT SSS sodium solute transporter superfamily OS=Imperialibacter sp. EC-SDR9 OX=2038371 GN=IMPR6_200016 PE=3 SV=1
MM1 pKa = 8.13DD2 pKa = 3.94VNRR5 pKa = 11.84LNNLLLLLFLLMVTSCDD22 pKa = 3.55QEE24 pKa = 5.12ADD26 pKa = 4.01GVDD29 pKa = 3.96KK30 pKa = 11.18DD31 pKa = 4.34CSGSGLTLTLLGSTDD46 pKa = 3.67TSCGASAGGFEE57 pKa = 4.54VEE59 pKa = 4.22ASGGFGGYY67 pKa = 9.4RR68 pKa = 11.84YY69 pKa = 10.29QIDD72 pKa = 3.98DD73 pKa = 3.97GAFQNSGVFSSVAPGKK89 pKa = 10.56YY90 pKa = 9.04IVGVEE95 pKa = 3.99DD96 pKa = 4.73SEE98 pKa = 5.07GCSSSLDD105 pKa = 3.47VQIFSGISFSASIKK119 pKa = 10.32PIIEE123 pKa = 4.24TNCAISGCHH132 pKa = 6.64DD133 pKa = 3.26GSGNIDD139 pKa = 3.45YY140 pKa = 11.47NNFDD144 pKa = 4.35NIKK147 pKa = 10.43LNPSDD152 pKa = 3.41IKK154 pKa = 11.29SRR156 pKa = 11.84TQSGDD161 pKa = 2.85MPRR164 pKa = 11.84NGTLTVAEE172 pKa = 4.71IDD174 pKa = 5.11MIACWVDD181 pKa = 4.6DD182 pKa = 3.92GALNNN187 pKa = 4.09

Molecular weight: 19.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E7YMH4|A0A5E7YMH4_9BACT Anthranilate synthase component I OS=Imperialibacter sp. EC-SDR9 OX=2038371 GN=IMPR6_160133 PE=3 SV=1
MM1 pKa = 7.44EE2 pKa = 6.22NIVSDD7 pKa = 4.07NNNHH11 pKa = 5.51FRR13 pKa = 11.84LIMKK17 pKa = 9.63IRR19 pKa = 11.84SAQWQDD25 pKa = 2.65GGTSHH30 pKa = 7.29TPLTQRR36 pKa = 11.84LGQMFNIMPIFRR48 pKa = 11.84AYY50 pKa = 10.35RR51 pKa = 11.84IKK53 pKa = 10.28DD54 pKa = 3.58TNNLNSLSVNAYY66 pKa = 10.22DD67 pKa = 5.24SEE69 pKa = 4.9SKK71 pKa = 10.58CGRR74 pKa = 11.84SPQARR79 pKa = 11.84CKK81 pKa = 10.29SRR83 pKa = 11.84RR84 pKa = 11.84AGQRR88 pKa = 11.84NN89 pKa = 3.22

Molecular weight: 10.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5735

0

5735

2035285

21

4165

354.9

39.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.433 ± 0.033

0.687 ± 0.01

5.538 ± 0.026

6.395 ± 0.031

5.077 ± 0.024

7.304 ± 0.037

1.819 ± 0.016

6.596 ± 0.026

6.365 ± 0.034

9.595 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.019

4.799 ± 0.027

3.911 ± 0.02

3.44 ± 0.017

4.081 ± 0.023

6.866 ± 0.031

5.636 ± 0.04

6.895 ± 0.027

1.308 ± 0.013

3.845 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski