Amino acid dipepetide frequency for Imperialibacter sp. EC-SDR9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.032AlaAla: 6.032 ± 0.077
0.648AlaCys: 0.648 ± 0.019
4.289AlaAsp: 4.289 ± 0.058
4.506AlaGlu: 4.506 ± 0.054
3.848AlaPhe: 3.848 ± 0.048
5.922AlaGly: 5.922 ± 0.075
1.218AlaHis: 1.218 ± 0.03
5.141AlaIle: 5.141 ± 0.06
4.187AlaLys: 4.187 ± 0.05
7.132AlaLeu: 7.132 ± 0.08
1.89AlaMet: 1.89 ± 0.035
3.331AlaAsn: 3.331 ± 0.05
2.536AlaPro: 2.536 ± 0.04
2.471AlaGln: 2.471 ± 0.033
2.87AlaArg: 2.87 ± 0.041
5.298AlaSer: 5.298 ± 0.065
4.347AlaThr: 4.347 ± 0.067
5.065AlaVal: 5.065 ± 0.058
0.91AlaTrp: 0.91 ± 0.023
2.693AlaTyr: 2.693 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
0.445CysAla: 0.445 ± 0.016
0.098CysCys: 0.098 ± 0.007
0.376CysAsp: 0.376 ± 0.014
0.425CysGlu: 0.425 ± 0.017
0.394CysPhe: 0.394 ± 0.015
0.565CysGly: 0.565 ± 0.02
0.205CysHis: 0.205 ± 0.011
0.465CysIle: 0.465 ± 0.015
0.332CysLys: 0.332 ± 0.014
0.697CysLeu: 0.697 ± 0.02
0.159CysMet: 0.159 ± 0.009
0.281CysAsn: 0.281 ± 0.013
0.259CysPro: 0.259 ± 0.012
0.263CysGln: 0.263 ± 0.011
0.31CysArg: 0.31 ± 0.011
0.501CysSer: 0.501 ± 0.016
0.356CysThr: 0.356 ± 0.015
0.412CysVal: 0.412 ± 0.017
0.084CysTrp: 0.084 ± 0.006
0.247CysTyr: 0.247 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
3.789AspAla: 3.789 ± 0.057
0.347AspCys: 0.347 ± 0.014
2.943AspAsp: 2.943 ± 0.052
3.739AspGlu: 3.739 ± 0.048
3.337AspPhe: 3.337 ± 0.047
4.474AspGly: 4.474 ± 0.072
0.975AspHis: 0.975 ± 0.025
3.83AspIle: 3.83 ± 0.046
3.571AspLys: 3.571 ± 0.048
5.276AspLeu: 5.276 ± 0.057
1.265AspMet: 1.265 ± 0.023
2.589AspAsn: 2.589 ± 0.045
2.405AspPro: 2.405 ± 0.042
1.937AspGln: 1.937 ± 0.029
2.244AspArg: 2.244 ± 0.032
3.258AspSer: 3.258 ± 0.044
2.529AspThr: 2.529 ± 0.037
3.508AspVal: 3.508 ± 0.043
0.836AspTrp: 0.836 ± 0.024
2.525AspTyr: 2.525 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
5.134GluAla: 5.134 ± 0.063
0.289GluCys: 0.289 ± 0.011
3.069GluAsp: 3.069 ± 0.036
4.722GluGlu: 4.722 ± 0.063
2.7GluPhe: 2.7 ± 0.039
4.356GluGly: 4.356 ± 0.056
1.078GluHis: 1.078 ± 0.022
4.389GluIle: 4.389 ± 0.051
5.154GluLys: 5.154 ± 0.058
6.215GluLeu: 6.215 ± 0.056
1.783GluMet: 1.783 ± 0.031
3.222GluAsn: 3.222 ± 0.038
2.008GluPro: 2.008 ± 0.037
2.269GluGln: 2.269 ± 0.036
2.823GluArg: 2.823 ± 0.037
3.704GluSer: 3.704 ± 0.044
3.206GluThr: 3.206 ± 0.04
4.87GluVal: 4.87 ± 0.057
0.828GluTrp: 0.828 ± 0.021
2.127GluTyr: 2.127 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
3.342PheAla: 3.342 ± 0.039
0.396PheCys: 0.396 ± 0.014
3.265PheAsp: 3.265 ± 0.045
3.249PheGlu: 3.249 ± 0.038
2.678PhePhe: 2.678 ± 0.049
3.816PheGly: 3.816 ± 0.056
0.927PheHis: 0.927 ± 0.027
3.169PheIle: 3.169 ± 0.045
2.769PheLys: 2.769 ± 0.041
4.625PheLeu: 4.625 ± 0.061
1.1PheMet: 1.1 ± 0.024
2.547PheAsn: 2.547 ± 0.04
1.92PhePro: 1.92 ± 0.034
1.638PheGln: 1.638 ± 0.028
2.072PheArg: 2.072 ± 0.033
4.088PheSer: 4.088 ± 0.058
3.082PheThr: 3.082 ± 0.055
3.379PheVal: 3.379 ± 0.047
0.634PheTrp: 0.634 ± 0.021
2.072PheTyr: 2.072 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
5.127GlyAla: 5.127 ± 0.063
0.614GlyCys: 0.614 ± 0.021
3.973GlyAsp: 3.973 ± 0.061
4.146GlyGlu: 4.146 ± 0.043
3.852GlyPhe: 3.852 ± 0.044
5.602GlyGly: 5.602 ± 0.077
1.296GlyHis: 1.296 ± 0.028
5.125GlyIle: 5.125 ± 0.057
5.094GlyLys: 5.094 ± 0.054
6.953GlyLeu: 6.953 ± 0.071
1.888GlyMet: 1.888 ± 0.035
3.54GlyAsn: 3.54 ± 0.053
1.909GlyPro: 1.909 ± 0.034
2.539GlyGln: 2.539 ± 0.04
2.892GlyArg: 2.892 ± 0.042
4.912GlySer: 4.912 ± 0.065
4.345GlyThr: 4.345 ± 0.082
5.121GlyVal: 5.121 ± 0.055
1.078GlyTrp: 1.078 ± 0.024
3.032GlyTyr: 3.032 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
1.126HisAla: 1.126 ± 0.022
0.16HisCys: 0.16 ± 0.009
0.851HisAsp: 0.851 ± 0.02
0.999HisGlu: 0.999 ± 0.024
1.169HisPhe: 1.169 ± 0.025
1.264HisGly: 1.264 ± 0.03
0.526HisHis: 0.526 ± 0.016
1.212HisIle: 1.212 ± 0.026
0.966HisLys: 0.966 ± 0.024
1.947HisLeu: 1.947 ± 0.033
0.433HisMet: 0.433 ± 0.015
0.759HisAsn: 0.759 ± 0.02
1.054HisPro: 1.054 ± 0.023
0.723HisGln: 0.723 ± 0.02
0.76HisArg: 0.76 ± 0.021
1.154HisSer: 1.154 ± 0.021
0.922HisThr: 0.922 ± 0.023
1.081HisVal: 1.081 ± 0.024
0.266HisTrp: 0.266 ± 0.013
0.816HisTyr: 0.816 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.232IleAla: 5.232 ± 0.057
0.513IleCys: 0.513 ± 0.017
4.221IleAsp: 4.221 ± 0.05
4.392IleGlu: 4.392 ± 0.052
3.054IlePhe: 3.054 ± 0.048
4.866IleGly: 4.866 ± 0.054
1.224IleHis: 1.224 ± 0.024
4.075IleIle: 4.075 ± 0.054
3.974IleLys: 3.974 ± 0.049
5.559IleLeu: 5.559 ± 0.069
1.255IleMet: 1.255 ± 0.03
3.313IleAsn: 3.313 ± 0.046
2.753IlePro: 2.753 ± 0.038
2.076IleGln: 2.076 ± 0.031
2.858IleArg: 2.858 ± 0.039
4.993IleSer: 4.993 ± 0.057
3.956IleThr: 3.956 ± 0.063
4.489IleVal: 4.489 ± 0.05
0.761IleTrp: 0.761 ± 0.019
2.394IleTyr: 2.394 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
5.098LysAla: 5.098 ± 0.062
0.293LysCys: 0.293 ± 0.012
3.566LysAsp: 3.566 ± 0.045
5.055LysGlu: 5.055 ± 0.064
2.293LysPhe: 2.293 ± 0.038
4.427LysGly: 4.427 ± 0.053
1.044LysHis: 1.044 ± 0.024
4.098LysIle: 4.098 ± 0.055
4.86LysLys: 4.86 ± 0.066
5.788LysLeu: 5.788 ± 0.067
1.795LysMet: 1.795 ± 0.034
3.089LysAsn: 3.089 ± 0.041
2.377LysPro: 2.377 ± 0.041
2.042LysGln: 2.042 ± 0.031
2.585LysArg: 2.585 ± 0.04
3.849LysSer: 3.849 ± 0.045
3.486LysThr: 3.486 ± 0.045
4.709LysVal: 4.709 ± 0.047
0.825LysTrp: 0.825 ± 0.021
2.371LysTyr: 2.371 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
7.668LeuAla: 7.668 ± 0.078
0.679LeuCys: 0.679 ± 0.021
4.932LeuAsp: 4.932 ± 0.051
5.856LeuGlu: 5.856 ± 0.06
4.784LeuPhe: 4.784 ± 0.061
6.417LeuGly: 6.417 ± 0.071
1.639LeuHis: 1.639 ± 0.026
6.14LeuIle: 6.14 ± 0.068
6.422LeuLys: 6.422 ± 0.066
9.712LeuLeu: 9.712 ± 0.113
2.227LeuMet: 2.227 ± 0.038
4.391LeuAsn: 4.391 ± 0.046
4.108LeuPro: 4.108 ± 0.053
3.289LeuGln: 3.289 ± 0.044
3.876LeuArg: 3.876 ± 0.049
7.415LeuSer: 7.415 ± 0.068
5.787LeuThr: 5.787 ± 0.059
6.429LeuVal: 6.429 ± 0.067
1.059LeuTrp: 1.059 ± 0.028
3.12LeuTyr: 3.12 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.083MetAla: 2.083 ± 0.035
0.116MetCys: 0.116 ± 0.007
1.285MetAsp: 1.285 ± 0.027
1.574MetGlu: 1.574 ± 0.026
0.837MetPhe: 0.837 ± 0.021
1.734MetGly: 1.734 ± 0.03
0.438MetHis: 0.438 ± 0.014
1.516MetIle: 1.516 ± 0.032
2.106MetLys: 2.106 ± 0.036
2.196MetLeu: 2.196 ± 0.037
0.668MetMet: 0.668 ± 0.019
1.149MetAsn: 1.149 ± 0.025
1.098MetPro: 1.098 ± 0.025
0.82MetGln: 0.82 ± 0.022
1.035MetArg: 1.035 ± 0.02
1.521MetSer: 1.521 ± 0.027
1.328MetThr: 1.328 ± 0.025
1.703MetVal: 1.703 ± 0.032
0.228MetTrp: 0.228 ± 0.011
0.667MetTyr: 0.667 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.209AsnAla: 3.209 ± 0.042
0.303AsnCys: 0.303 ± 0.012
2.677AsnAsp: 2.677 ± 0.044
2.92AsnGlu: 2.92 ± 0.042
2.438AsnPhe: 2.438 ± 0.038
3.775AsnGly: 3.775 ± 0.053
0.876AsnHis: 0.876 ± 0.023
3.259AsnIle: 3.259 ± 0.051
2.738AsnLys: 2.738 ± 0.038
4.339AsnLeu: 4.339 ± 0.047
1.095AsnMet: 1.095 ± 0.026
2.429AsnAsn: 2.429 ± 0.053
2.461AsnPro: 2.461 ± 0.038
1.757AsnGln: 1.757 ± 0.031
2.036AsnArg: 2.036 ± 0.039
3.168AsnSer: 3.168 ± 0.052
2.677AsnThr: 2.677 ± 0.048
2.951AsnVal: 2.951 ± 0.05
0.704AsnTrp: 0.704 ± 0.02
2.179AsnTyr: 2.179 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
3.091ProAla: 3.091 ± 0.047
0.194ProCys: 0.194 ± 0.009
2.686ProAsp: 2.686 ± 0.039
3.089ProGlu: 3.089 ± 0.039
2.052ProPhe: 2.052 ± 0.034
3.089ProGly: 3.089 ± 0.047
0.723ProHis: 0.723 ± 0.02
2.379ProIle: 2.379 ± 0.033
2.114ProLys: 2.114 ± 0.033
3.361ProLeu: 3.361 ± 0.042
0.889ProMet: 0.889 ± 0.021
1.879ProAsn: 1.879 ± 0.037
1.226ProPro: 1.226 ± 0.029
1.18ProGln: 1.18 ± 0.022
1.202ProArg: 1.202 ± 0.024
2.621ProSer: 2.621 ± 0.043
2.24ProThr: 2.24 ± 0.037
3.115ProVal: 3.115 ± 0.042
0.516ProTrp: 0.516 ± 0.016
1.469ProTyr: 1.469 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
2.57GlnAla: 2.57 ± 0.035
0.17GlnCys: 0.17 ± 0.01
1.389GlnAsp: 1.389 ± 0.024
2.093GlnGlu: 2.093 ± 0.035
1.675GlnPhe: 1.675 ± 0.029
2.095GlnGly: 2.095 ± 0.035
0.627GlnHis: 0.627 ± 0.019
2.129GlnIle: 2.129 ± 0.028
2.372GlnLys: 2.372 ± 0.041
3.819GlnLeu: 3.819 ± 0.049
0.961GlnMet: 0.961 ± 0.024
1.594GlnAsn: 1.594 ± 0.031
1.51GlnPro: 1.51 ± 0.026
1.543GlnGln: 1.543 ± 0.033
1.519GlnArg: 1.519 ± 0.028
2.233GlnSer: 2.233 ± 0.039
1.832GlnThr: 1.832 ± 0.032
2.436GlnVal: 2.436 ± 0.035
0.501GlnTrp: 0.501 ± 0.018
1.332GlnTyr: 1.332 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
2.527ArgAla: 2.527 ± 0.04
0.221ArgCys: 0.221 ± 0.01
1.976ArgAsp: 1.976 ± 0.034
2.69ArgGlu: 2.69 ± 0.038
2.191ArgPhe: 2.191 ± 0.037
2.326ArgGly: 2.326 ± 0.035
0.79ArgHis: 0.79 ± 0.021
2.916ArgIle: 2.916 ± 0.045
3.009ArgLys: 3.009 ± 0.041
4.226ArgLeu: 4.226 ± 0.05
1.186ArgMet: 1.186 ± 0.025
2.173ArgAsn: 2.173 ± 0.033
1.511ArgPro: 1.511 ± 0.032
1.611ArgGln: 1.611 ± 0.032
1.88ArgArg: 1.88 ± 0.038
2.533ArgSer: 2.533 ± 0.037
2.085ArgThr: 2.085 ± 0.033
2.694ArgVal: 2.694 ± 0.035
0.584ArgTrp: 0.584 ± 0.017
1.681ArgTyr: 1.681 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
4.783SerAla: 4.783 ± 0.069
0.557SerCys: 0.557 ± 0.014
3.633SerAsp: 3.633 ± 0.046
3.899SerGlu: 3.899 ± 0.045
4.062SerPhe: 4.062 ± 0.049
5.469SerGly: 5.469 ± 0.072
1.278SerHis: 1.278 ± 0.027
4.665SerIle: 4.665 ± 0.046
3.812SerLys: 3.812 ± 0.047
7.017SerLeu: 7.017 ± 0.06
1.557SerMet: 1.557 ± 0.029
3.121SerAsn: 3.121 ± 0.051
2.716SerPro: 2.716 ± 0.047
2.432SerGln: 2.432 ± 0.039
2.657SerArg: 2.657 ± 0.041
4.875SerSer: 4.875 ± 0.076
3.851SerThr: 3.851 ± 0.051
4.588SerVal: 4.588 ± 0.054
0.948SerTrp: 0.948 ± 0.021
2.738SerTyr: 2.738 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
4.206ThrAla: 4.206 ± 0.061
0.352ThrCys: 0.352 ± 0.015
3.282ThrAsp: 3.282 ± 0.056
3.222ThrGlu: 3.222 ± 0.047
2.956ThrPhe: 2.956 ± 0.048
4.535ThrGly: 4.535 ± 0.072
1.017ThrHis: 1.017 ± 0.022
4.073ThrIle: 4.073 ± 0.059
2.904ThrLys: 2.904 ± 0.041
5.481ThrLeu: 5.481 ± 0.053
1.135ThrMet: 1.135 ± 0.025
2.539ThrAsn: 2.539 ± 0.05
2.533ThrPro: 2.533 ± 0.039
1.708ThrGln: 1.708 ± 0.029
1.963ThrArg: 1.963 ± 0.033
4.025ThrSer: 4.025 ± 0.068
3.374ThrThr: 3.374 ± 0.06
4.03ThrVal: 4.03 ± 0.074
0.759ThrTrp: 0.759 ± 0.025
2.268ThrTyr: 2.268 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
5.454ValAla: 5.454 ± 0.06
0.529ValCys: 0.529 ± 0.018
3.946ValAsp: 3.946 ± 0.051
4.29ValGlu: 4.29 ± 0.052
3.523ValPhe: 3.523 ± 0.043
4.704ValGly: 4.704 ± 0.055
1.154ValHis: 1.154 ± 0.026
4.55ValIle: 4.55 ± 0.056
4.175ValLys: 4.175 ± 0.055
6.424ValLeu: 6.424 ± 0.068
1.643ValMet: 1.643 ± 0.029
3.506ValAsn: 3.506 ± 0.052
2.669ValPro: 2.669 ± 0.039
1.913ValGln: 1.913 ± 0.034
2.789ValArg: 2.789 ± 0.04
5.06ValSer: 5.06 ± 0.063
4.141ValThr: 4.141 ± 0.079
5.203ValVal: 5.203 ± 0.068
0.786ValTrp: 0.786 ± 0.021
2.491ValTyr: 2.491 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
0.914TrpAla: 0.914 ± 0.022
0.092TrpCys: 0.092 ± 0.007
0.736TrpAsp: 0.736 ± 0.023
0.813TrpGlu: 0.813 ± 0.021
0.589TrpPhe: 0.589 ± 0.019
0.923TrpGly: 0.923 ± 0.022
0.301TrpHis: 0.301 ± 0.013
0.735TrpIle: 0.735 ± 0.022
0.889TrpLys: 0.889 ± 0.02
1.334TrpLeu: 1.334 ± 0.032
0.395TrpMet: 0.395 ± 0.015
0.672TrpAsn: 0.672 ± 0.019
0.425TrpPro: 0.425 ± 0.016
0.533TrpGln: 0.533 ± 0.017
0.579TrpArg: 0.579 ± 0.016
0.809TrpSer: 0.809 ± 0.021
0.727TrpThr: 0.727 ± 0.023
0.903TrpVal: 0.903 ± 0.024
0.231TrpTrp: 0.231 ± 0.009
0.48TrpTyr: 0.48 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.504TyrAla: 2.504 ± 0.045
0.304TyrCys: 0.304 ± 0.013
2.282TyrAsp: 2.282 ± 0.035
2.268TyrGlu: 2.268 ± 0.036
2.334TyrPhe: 2.334 ± 0.032
2.679TyrGly: 2.679 ± 0.036
0.85TyrHis: 0.85 ± 0.023
2.094TyrIle: 2.094 ± 0.033
2.203TyrLys: 2.203 ± 0.039
3.845TyrLeu: 3.845 ± 0.047
0.782TyrMet: 0.782 ± 0.019
1.869TyrAsn: 1.869 ± 0.034
1.636TyrPro: 1.636 ± 0.032
1.656TyrGln: 1.656 ± 0.031
1.859TyrArg: 1.859 ± 0.037
2.642TyrSer: 2.642 ± 0.041
2.091TyrThr: 2.091 ± 0.038
2.264TyrVal: 2.264 ± 0.038
0.54TyrTrp: 0.54 ± 0.016
1.745TyrTyr: 1.745 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5735 proteins (2035285 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski