Amino acid dipepetide frequency for Rhizobium sp. CF142

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.565AlaAla: 15.565 ± 0.113
0.969AlaCys: 0.969 ± 0.021
6.714AlaAsp: 6.714 ± 0.062
7.221AlaGlu: 7.221 ± 0.07
4.623AlaPhe: 4.623 ± 0.049
9.867AlaGly: 9.867 ± 0.073
2.048AlaHis: 2.048 ± 0.032
7.082AlaIle: 7.082 ± 0.072
4.364AlaLys: 4.364 ± 0.05
12.324AlaLeu: 12.324 ± 0.09
3.38AlaMet: 3.38 ± 0.036
3.166AlaAsn: 3.166 ± 0.04
4.652AlaPro: 4.652 ± 0.059
3.428AlaGln: 3.428 ± 0.045
7.192AlaArg: 7.192 ± 0.066
6.659AlaSer: 6.659 ± 0.056
5.744AlaThr: 5.744 ± 0.052
8.42AlaVal: 8.42 ± 0.07
1.37AlaTrp: 1.37 ± 0.028
2.617AlaTyr: 2.617 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
0.851CysAla: 0.851 ± 0.02
0.12CysCys: 0.12 ± 0.007
0.465CysAsp: 0.465 ± 0.014
0.419CysGlu: 0.419 ± 0.015
0.318CysPhe: 0.318 ± 0.012
0.855CysGly: 0.855 ± 0.021
0.242CysHis: 0.242 ± 0.012
0.408CysIle: 0.408 ± 0.011
0.194CysLys: 0.194 ± 0.01
0.809CysLeu: 0.809 ± 0.019
0.169CysMet: 0.169 ± 0.009
0.202CysAsn: 0.202 ± 0.01
0.36CysPro: 0.36 ± 0.013
0.22CysGln: 0.22 ± 0.01
0.549CysArg: 0.549 ± 0.016
0.475CysSer: 0.475 ± 0.016
0.383CysThr: 0.383 ± 0.013
0.552CysVal: 0.552 ± 0.016
0.107CysTrp: 0.107 ± 0.007
0.196CysTyr: 0.196 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
6.368AspAla: 6.368 ± 0.053
0.471AspCys: 0.471 ± 0.016
3.057AspAsp: 3.057 ± 0.04
3.484AspGlu: 3.484 ± 0.038
2.378AspPhe: 2.378 ± 0.036
5.026AspGly: 5.026 ± 0.062
1.286AspHis: 1.286 ± 0.025
3.712AspIle: 3.712 ± 0.045
1.936AspLys: 1.936 ± 0.035
5.699AspLeu: 5.699 ± 0.054
1.424AspMet: 1.424 ± 0.026
1.572AspAsn: 1.572 ± 0.028
3.195AspPro: 3.195 ± 0.046
1.743AspGln: 1.743 ± 0.028
3.964AspArg: 3.964 ± 0.042
2.225AspSer: 2.225 ± 0.033
2.536AspThr: 2.536 ± 0.044
4.142AspVal: 4.142 ± 0.046
0.951AspTrp: 0.951 ± 0.022
1.623AspTyr: 1.623 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.128GluAla: 7.128 ± 0.066
0.327GluCys: 0.327 ± 0.012
2.844GluAsp: 2.844 ± 0.036
3.508GluGlu: 3.508 ± 0.055
1.936GluPhe: 1.936 ± 0.033
4.142GluGly: 4.142 ± 0.046
1.171GluHis: 1.171 ± 0.022
3.832GluIle: 3.832 ± 0.044
2.724GluLys: 2.724 ± 0.04
5.376GluLeu: 5.376 ± 0.054
1.581GluMet: 1.581 ± 0.027
1.874GluAsn: 1.874 ± 0.029
2.571GluPro: 2.571 ± 0.033
2.106GluGln: 2.106 ± 0.032
4.535GluArg: 4.535 ± 0.049
2.431GluSer: 2.431 ± 0.031
3.59GluThr: 3.59 ± 0.042
3.637GluVal: 3.637 ± 0.039
0.71GluTrp: 0.71 ± 0.017
1.039GluTyr: 1.039 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
4.692PheAla: 4.692 ± 0.047
0.414PheCys: 0.414 ± 0.014
2.758PheAsp: 2.758 ± 0.038
2.255PheGlu: 2.255 ± 0.034
1.662PhePhe: 1.662 ± 0.034
3.78PheGly: 3.78 ± 0.048
0.831PheHis: 0.831 ± 0.018
2.046PheIle: 2.046 ± 0.032
1.188PheLys: 1.188 ± 0.025
3.664PheLeu: 3.664 ± 0.051
0.888PheMet: 0.888 ± 0.02
1.249PheAsn: 1.249 ± 0.022
1.686PhePro: 1.686 ± 0.028
1.13PheGln: 1.13 ± 0.021
2.343PheArg: 2.343 ± 0.035
2.626PheSer: 2.626 ± 0.034
2.06PheThr: 2.06 ± 0.035
3.034PheVal: 3.034 ± 0.034
0.593PheTrp: 0.593 ± 0.017
1.057PheTyr: 1.057 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
8.25GlyAla: 8.25 ± 0.065
0.806GlyCys: 0.806 ± 0.02
4.204GlyAsp: 4.204 ± 0.055
4.6GlyGlu: 4.6 ± 0.048
3.722GlyPhe: 3.722 ± 0.04
6.656GlyGly: 6.656 ± 0.074
1.828GlyHis: 1.828 ± 0.031
5.188GlyIle: 5.188 ± 0.051
3.742GlyLys: 3.742 ± 0.048
8.614GlyLeu: 8.614 ± 0.058
2.288GlyMet: 2.288 ± 0.032
2.507GlyAsn: 2.507 ± 0.045
3.175GlyPro: 3.175 ± 0.041
2.703GlyGln: 2.703 ± 0.04
5.533GlyArg: 5.533 ± 0.058
4.822GlySer: 4.822 ± 0.052
4.412GlyThr: 4.412 ± 0.06
5.582GlyVal: 5.582 ± 0.055
1.304GlyTrp: 1.304 ± 0.024
2.402GlyTyr: 2.402 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.157HisAla: 2.157 ± 0.031
0.2HisCys: 0.2 ± 0.009
1.268HisAsp: 1.268 ± 0.025
1.074HisGlu: 1.074 ± 0.023
0.96HisPhe: 0.96 ± 0.024
1.818HisGly: 1.818 ± 0.031
0.562HisHis: 0.562 ± 0.018
1.078HisIle: 1.078 ± 0.025
0.523HisLys: 0.523 ± 0.016
2.147HisLeu: 2.147 ± 0.032
0.56HisMet: 0.56 ± 0.015
0.47HisAsn: 0.47 ± 0.014
1.248HisPro: 1.248 ± 0.026
0.635HisGln: 0.635 ± 0.017
1.365HisArg: 1.365 ± 0.025
1.026HisSer: 1.026 ± 0.023
0.808HisThr: 0.808 ± 0.019
1.49HisVal: 1.49 ± 0.028
0.318HisTrp: 0.318 ± 0.012
0.563HisTyr: 0.563 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
7.987IleAla: 7.987 ± 0.068
0.546IleCys: 0.546 ± 0.017
3.899IleAsp: 3.899 ± 0.037
3.813IleGlu: 3.813 ± 0.049
2.223IlePhe: 2.223 ± 0.033
5.517IleGly: 5.517 ± 0.056
1.012IleHis: 1.012 ± 0.019
3.009IleIle: 3.009 ± 0.044
1.747IleLys: 1.747 ± 0.029
5.108IleLeu: 5.108 ± 0.053
1.204IleMet: 1.204 ± 0.023
1.688IleAsn: 1.688 ± 0.031
2.612IlePro: 2.612 ± 0.039
1.335IleGln: 1.335 ± 0.026
3.625IleArg: 3.625 ± 0.04
3.761IleSer: 3.761 ± 0.041
2.946IleThr: 2.946 ± 0.042
4.883IleVal: 4.883 ± 0.046
0.7IleTrp: 0.7 ± 0.018
1.336IleTyr: 1.336 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
4.736LysAla: 4.736 ± 0.051
0.164LysCys: 0.164 ± 0.008
2.039LysAsp: 2.039 ± 0.029
1.897LysGlu: 1.897 ± 0.032
1.07LysPhe: 1.07 ± 0.025
2.857LysGly: 2.857 ± 0.04
0.684LysHis: 0.684 ± 0.018
2.12LysIle: 2.12 ± 0.033
1.538LysLys: 1.538 ± 0.033
3.795LysLeu: 3.795 ± 0.046
0.913LysMet: 0.913 ± 0.022
1.079LysAsn: 1.079 ± 0.022
2.231LysPro: 2.231 ± 0.033
1.182LysGln: 1.182 ± 0.02
2.468LysArg: 2.468 ± 0.033
2.255LysSer: 2.255 ± 0.037
2.266LysThr: 2.266 ± 0.031
2.671LysVal: 2.671 ± 0.038
0.4LysTrp: 0.4 ± 0.015
0.708LysTyr: 0.708 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
12.522LeuAla: 12.522 ± 0.09
0.846LeuCys: 0.846 ± 0.022
5.903LeuAsp: 5.903 ± 0.055
5.161LeuGlu: 5.161 ± 0.055
3.895LeuPhe: 3.895 ± 0.049
7.827LeuGly: 7.827 ± 0.068
1.843LeuHis: 1.843 ± 0.035
5.46LeuIle: 5.46 ± 0.06
4.073LeuLys: 4.073 ± 0.042
9.587LeuLeu: 9.587 ± 0.095
2.411LeuMet: 2.411 ± 0.035
2.782LeuAsn: 2.782 ± 0.041
5.352LeuPro: 5.352 ± 0.061
3.036LeuGln: 3.036 ± 0.042
6.076LeuArg: 6.076 ± 0.064
7.14LeuSer: 7.14 ± 0.069
5.68LeuThr: 5.68 ± 0.058
7.217LeuVal: 7.217 ± 0.066
1.107LeuTrp: 1.107 ± 0.024
2.208LeuTyr: 2.208 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.996MetAla: 2.996 ± 0.041
0.137MetCys: 0.137 ± 0.007
1.172MetAsp: 1.172 ± 0.024
1.235MetGlu: 1.235 ± 0.025
0.751MetPhe: 0.751 ± 0.019
1.703MetGly: 1.703 ± 0.028
0.451MetHis: 0.451 ± 0.014
1.591MetIle: 1.591 ± 0.027
1.137MetLys: 1.137 ± 0.025
2.555MetLeu: 2.555 ± 0.037
0.71MetMet: 0.71 ± 0.019
0.87MetAsn: 0.87 ± 0.02
1.484MetPro: 1.484 ± 0.03
0.936MetGln: 0.936 ± 0.023
1.813MetArg: 1.813 ± 0.033
1.828MetSer: 1.828 ± 0.029
2.009MetThr: 2.009 ± 0.031
1.742MetVal: 1.742 ± 0.029
0.186MetTrp: 0.186 ± 0.009
0.333MetTyr: 0.333 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.403AsnAla: 3.403 ± 0.038
0.233AsnCys: 0.233 ± 0.01
1.606AsnAsp: 1.606 ± 0.041
1.47AsnGlu: 1.47 ± 0.026
1.183AsnPhe: 1.183 ± 0.026
2.657AsnGly: 2.657 ± 0.046
0.578AsnHis: 0.578 ± 0.019
1.671AsnIle: 1.671 ± 0.029
0.851AsnLys: 0.851 ± 0.019
2.849AsnLeu: 2.849 ± 0.039
0.716AsnMet: 0.716 ± 0.02
0.871AsnAsn: 0.871 ± 0.023
1.892AsnPro: 1.892 ± 0.03
0.868AsnGln: 0.868 ± 0.019
1.931AsnArg: 1.931 ± 0.032
1.552AsnSer: 1.552 ± 0.025
1.393AsnThr: 1.393 ± 0.028
2.093AsnVal: 2.093 ± 0.034
0.492AsnTrp: 0.492 ± 0.015
0.805AsnTyr: 0.805 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
5.609ProAla: 5.609 ± 0.061
0.262ProCys: 0.262 ± 0.011
3.453ProAsp: 3.453 ± 0.044
3.503ProGlu: 3.503 ± 0.038
1.972ProPhe: 1.972 ± 0.028
3.988ProGly: 3.988 ± 0.048
1.085ProHis: 1.085 ± 0.019
2.467ProIle: 2.467 ± 0.034
1.813ProLys: 1.813 ± 0.032
4.618ProLeu: 4.618 ± 0.048
1.125ProMet: 1.125 ± 0.023
1.399ProAsn: 1.399 ± 0.023
2.193ProPro: 2.193 ± 0.037
1.659ProGln: 1.659 ± 0.032
2.477ProArg: 2.477 ± 0.037
2.843ProSer: 2.843 ± 0.035
2.376ProThr: 2.376 ± 0.037
4.129ProVal: 4.129 ± 0.049
0.617ProTrp: 0.617 ± 0.018
1.242ProTyr: 1.242 ± 0.023
0.0ProXaa: 0.0 ± 0.0
Gln
3.85GlnAla: 3.85 ± 0.05
0.18GlnCys: 0.18 ± 0.01
1.462GlnAsp: 1.462 ± 0.03
1.567GlnGlu: 1.567 ± 0.028
1.154GlnPhe: 1.154 ± 0.022
2.185GlnGly: 2.185 ± 0.036
0.589GlnHis: 0.589 ± 0.019
1.988GlnIle: 1.988 ± 0.029
1.275GlnLys: 1.275 ± 0.027
2.894GlnLeu: 2.894 ± 0.036
0.958GlnMet: 0.958 ± 0.022
0.973GlnAsn: 0.973 ± 0.021
1.795GlnPro: 1.795 ± 0.028
1.389GlnGln: 1.389 ± 0.037
2.234GlnArg: 2.234 ± 0.034
1.974GlnSer: 1.974 ± 0.031
1.793GlnThr: 1.793 ± 0.031
2.06GlnVal: 2.06 ± 0.035
0.415GlnTrp: 0.415 ± 0.015
0.685GlnTyr: 0.685 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
6.506ArgAla: 6.506 ± 0.059
0.423ArgCys: 0.423 ± 0.013
3.724ArgAsp: 3.724 ± 0.041
3.837ArgGlu: 3.837 ± 0.046
2.8ArgPhe: 2.8 ± 0.034
4.227ArgGly: 4.227 ± 0.051
1.636ArgHis: 1.636 ± 0.03
4.071ArgIle: 4.071 ± 0.043
2.494ArgLys: 2.494 ± 0.035
7.314ArgLeu: 7.314 ± 0.07
1.893ArgMet: 1.893 ± 0.031
1.999ArgAsn: 1.999 ± 0.03
3.094ArgPro: 3.094 ± 0.038
2.588ArgGln: 2.588 ± 0.039
4.899ArgArg: 4.899 ± 0.052
3.767ArgSer: 3.767 ± 0.042
3.142ArgThr: 3.142 ± 0.04
4.108ArgVal: 4.108 ± 0.05
0.914ArgTrp: 0.914 ± 0.021
1.686ArgTyr: 1.686 ± 0.026
0.0ArgXaa: 0.0 ± 0.0
Ser
6.511SerAla: 6.511 ± 0.055
0.425SerCys: 0.425 ± 0.013
3.198SerAsp: 3.198 ± 0.042
3.123SerGlu: 3.123 ± 0.042
2.623SerPhe: 2.623 ± 0.037
5.939SerGly: 5.939 ± 0.069
1.197SerHis: 1.197 ± 0.023
3.439SerIle: 3.439 ± 0.039
1.932SerLys: 1.932 ± 0.031
6.055SerLeu: 6.055 ± 0.059
1.473SerMet: 1.473 ± 0.027
1.662SerAsn: 1.662 ± 0.028
2.878SerPro: 2.878 ± 0.041
1.716SerGln: 1.716 ± 0.031
3.814SerArg: 3.814 ± 0.039
3.476SerSer: 3.476 ± 0.047
2.961SerThr: 2.961 ± 0.039
4.375SerVal: 4.375 ± 0.044
0.807SerTrp: 0.807 ± 0.018
1.467SerTyr: 1.467 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
6.054ThrAla: 6.054 ± 0.055
0.403ThrCys: 0.403 ± 0.013
2.887ThrAsp: 2.887 ± 0.037
2.727ThrGlu: 2.727 ± 0.038
2.167ThrPhe: 2.167 ± 0.03
4.958ThrGly: 4.958 ± 0.054
1.043ThrHis: 1.043 ± 0.021
3.385ThrIle: 3.385 ± 0.045
1.697ThrLys: 1.697 ± 0.029
5.571ThrLeu: 5.571 ± 0.054
1.266ThrMet: 1.266 ± 0.026
1.44ThrAsn: 1.44 ± 0.03
2.966ThrPro: 2.966 ± 0.037
1.396ThrGln: 1.396 ± 0.026
3.046ThrArg: 3.046 ± 0.038
3.148ThrSer: 3.148 ± 0.039
2.897ThrThr: 2.897 ± 0.037
4.402ThrVal: 4.402 ± 0.048
0.645ThrTrp: 0.645 ± 0.017
1.268ThrTyr: 1.268 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
8.57ValAla: 8.57 ± 0.07
0.597ValCys: 0.597 ± 0.017
4.046ValAsp: 4.046 ± 0.046
4.293ValGlu: 4.293 ± 0.047
2.931ValPhe: 2.931 ± 0.036
5.296ValGly: 5.296 ± 0.049
1.356ValHis: 1.356 ± 0.022
4.555ValIle: 4.555 ± 0.053
2.57ValLys: 2.57 ± 0.039
7.17ValLeu: 7.17 ± 0.062
1.828ValMet: 1.828 ± 0.028
2.109ValAsn: 2.109 ± 0.032
3.539ValPro: 3.539 ± 0.042
1.99ValGln: 1.99 ± 0.035
4.47ValArg: 4.47 ± 0.05
4.849ValSer: 4.849 ± 0.043
4.468ValThr: 4.468 ± 0.04
5.674ValVal: 5.674 ± 0.059
0.867ValTrp: 0.867 ± 0.022
1.51ValTyr: 1.51 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.123TrpAla: 1.123 ± 0.024
0.125TrpCys: 0.125 ± 0.007
0.613TrpAsp: 0.613 ± 0.017
0.571TrpGlu: 0.571 ± 0.017
0.534TrpPhe: 0.534 ± 0.015
0.857TrpGly: 0.857 ± 0.021
0.324TrpHis: 0.324 ± 0.012
0.747TrpIle: 0.747 ± 0.02
0.546TrpLys: 0.546 ± 0.019
1.59TrpLeu: 1.59 ± 0.028
0.355TrpMet: 0.355 ± 0.014
0.516TrpAsn: 0.516 ± 0.016
0.692TrpPro: 0.692 ± 0.018
0.583TrpGln: 0.583 ± 0.017
1.021TrpArg: 1.021 ± 0.023
0.861TrpSer: 0.861 ± 0.024
0.77TrpThr: 0.77 ± 0.018
0.766TrpVal: 0.766 ± 0.019
0.223TrpTrp: 0.223 ± 0.011
0.315TrpTyr: 0.315 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.527TyrAla: 2.527 ± 0.033
0.246TyrCys: 0.246 ± 0.01
1.479TyrAsp: 1.479 ± 0.026
1.305TyrGlu: 1.305 ± 0.024
1.052TyrPhe: 1.052 ± 0.021
2.183TyrGly: 2.183 ± 0.031
0.505TyrHis: 0.505 ± 0.016
1.103TyrIle: 1.103 ± 0.024
0.73TyrLys: 0.73 ± 0.017
2.386TyrLeu: 2.386 ± 0.038
0.498TyrMet: 0.498 ± 0.015
0.687TyrAsn: 0.687 ± 0.019
1.215TyrPro: 1.215 ± 0.022
0.776TyrGln: 0.776 ± 0.019
1.767TyrArg: 1.767 ± 0.034
1.353TyrSer: 1.353 ± 0.027
1.131TyrThr: 1.131 ± 0.024
1.71TyrVal: 1.71 ± 0.028
0.407TyrTrp: 0.407 ± 0.015
0.684TyrTyr: 0.684 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7229 proteins (2195810 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski