Terrimicrobium sacchariphilum

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Terrimicrobia; Terrimicrobiales; Terrimicrobiaceae; Terrimicrobium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A146GEE6|A0A146GEE6_TERSA UPF0234 protein TSACC_3114 OS=Terrimicrobium sacchariphilum OX=690879 GN=TSACC_3114 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.45GKK4 pKa = 10.05LARR7 pKa = 11.84TGGWIGVLSLLAAGMAAAAWNDD29 pKa = 3.47PVTNTPAIIQLDD41 pKa = 4.02FGPYY45 pKa = 9.37GNKK48 pKa = 10.26LPGDD52 pKa = 3.77GQNYY56 pKa = 9.52CEE58 pKa = 4.25PTSAVMSLFWLGSNGFTQLTPSTYY82 pKa = 10.0PGQNDD87 pKa = 4.27PYY89 pKa = 9.62TYY91 pKa = 10.86NLEE94 pKa = 4.05RR95 pKa = 11.84LMGGLMQTSATQGTYY110 pKa = 10.53DD111 pKa = 3.11SSAIQGISDD120 pKa = 3.56YY121 pKa = 10.97LAAAGLSGSFSYY133 pKa = 10.15IYY135 pKa = 10.69NGGDD139 pKa = 3.27SASNLLNPSWLAAQLAPNAGANPGSITFIDD169 pKa = 5.97FSISWYY175 pKa = 9.04GQNSGSSTSFSAEE188 pKa = 3.89GGHH191 pKa = 6.15EE192 pKa = 4.09LALLAVVDD200 pKa = 4.15PTGNAQLILNNAYY213 pKa = 9.61PSTLEE218 pKa = 3.81NVPNVPEE225 pKa = 4.95SDD227 pKa = 3.68PQTLGITQVPAGWNLPGMALPSEE250 pKa = 5.63DD251 pKa = 3.41YY252 pKa = 10.06WQLQSSDD259 pKa = 3.35GTVGDD264 pKa = 4.08GSTTKK269 pKa = 10.74AIVDD273 pKa = 3.52SAYY276 pKa = 10.57AFSVDD281 pKa = 3.16TMANPTLNPSWTPADD296 pKa = 3.52WVISGSKK303 pKa = 9.15TINTNSATFTVQAAVTGAGGFNKK326 pKa = 9.71TGEE329 pKa = 4.3GALVLTAGDD338 pKa = 4.6DD339 pKa = 3.77LTGNNAVSGGALGSTNAGDD358 pKa = 3.94STPLGTGSFVLTNGGTIGLTPAGSGGPLAFSIASGSGSSVTFGAGGGALEE408 pKa = 4.63IDD410 pKa = 3.16AGGYY414 pKa = 10.37DD415 pKa = 3.86SLSVTMGDD423 pKa = 4.02GSATSSNLIRR433 pKa = 11.84QSAGTFVIIPAHH445 pKa = 6.61GISALGTTEE454 pKa = 3.75RR455 pKa = 11.84VLVAGGSSSRR465 pKa = 11.84PASVNGMVAPYY476 pKa = 10.12IVGQDD481 pKa = 3.22NDD483 pKa = 4.26AGASGYY489 pKa = 9.82FLNYY493 pKa = 9.83DD494 pKa = 3.95GSAGFQKK501 pKa = 10.61AAAVSSLSTAIDD513 pKa = 3.51VATSSTVYY521 pKa = 10.44AAEE524 pKa = 4.11TDD526 pKa = 3.6QVIATNEE533 pKa = 3.96TARR536 pKa = 11.84VYY538 pKa = 10.91ALQVNDD544 pKa = 4.28SEE546 pKa = 5.08VSGTNATLEE555 pKa = 4.23VGSRR559 pKa = 11.84QAGDD563 pKa = 3.35SAGVILNGGTISASSLDD580 pKa = 3.51FGAAEE585 pKa = 4.09GVIYY589 pKa = 10.56ASEE592 pKa = 4.2AGGAISSTITSSAGLTTFGPGTVALSGDD620 pKa = 3.85STSTLSGTVTVNSGTLSAQSAQGSATGSANVSVQANATLSVSGAGAVAGNVSVQNNGTLLLSGGTISGNVTVAEE694 pKa = 4.5VGQSSANPGGTLAGSGTLSGSTTARR719 pKa = 11.84GVITSGASGGNITFEE734 pKa = 4.32GSADD738 pKa = 3.69LNSSQFFWQLDD749 pKa = 3.81SLTDD753 pKa = 3.82DD754 pKa = 4.24ASQAGIEE761 pKa = 4.19WNLLTFLGDD770 pKa = 3.44SHH772 pKa = 8.24VGSEE776 pKa = 4.28DD777 pKa = 3.71APVTIYY783 pKa = 10.9LQFAAGVITPDD794 pKa = 3.19TADD797 pKa = 4.11PFWMTDD803 pKa = 3.63HH804 pKa = 6.72EE805 pKa = 4.53WKK807 pKa = 10.2IWDD810 pKa = 4.1FSQATSYY817 pKa = 10.93SWDD820 pKa = 3.49DD821 pKa = 3.69SGDD824 pKa = 3.52GNTSFNYY831 pKa = 10.43GNFDD835 pKa = 3.83VVEE838 pKa = 4.27KK839 pKa = 10.76AADD842 pKa = 3.28NSVYY846 pKa = 10.43LKK848 pKa = 10.55YY849 pKa = 10.84VAVPEE854 pKa = 4.24PAVIWLALAGCGAMIASRR872 pKa = 11.84RR873 pKa = 11.84IRR875 pKa = 11.84QRR877 pKa = 11.84RR878 pKa = 3.26

Molecular weight: 88.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A146G4Z7|A0A146G4Z7_TERSA LacI family transcriptional regulator OS=Terrimicrobium sacchariphilum OX=690879 GN=TSACC_21070 PE=4 SV=1
MM1 pKa = 7.14LTRR4 pKa = 11.84RR5 pKa = 11.84EE6 pKa = 4.14NGTLPSFTLRR16 pKa = 11.84SDD18 pKa = 3.75TLPLSLRR25 pKa = 11.84HH26 pKa = 6.19AGRR29 pKa = 11.84SGRR32 pKa = 11.84GEE34 pKa = 4.05RR35 pKa = 11.84HH36 pKa = 5.72EE37 pKa = 4.2MTPPRR42 pKa = 11.84AGLWFRR48 pKa = 11.84ILAVEE53 pKa = 4.62TWQLVLLDD61 pKa = 4.74LYY63 pKa = 10.38IAAWAIGMVLQTLLVRR79 pKa = 11.84WLKK82 pKa = 11.07AKK84 pKa = 9.81TSEE87 pKa = 4.5EE88 pKa = 4.35LDD90 pKa = 3.37PHH92 pKa = 6.93LGPGARR98 pKa = 11.84AKK100 pKa = 10.88LSGYY104 pKa = 10.14SIASSMILLRR114 pKa = 11.84LIFTDD119 pKa = 3.94TPLSRR124 pKa = 11.84SPDD127 pKa = 3.28LRR129 pKa = 11.84TICLWLRR136 pKa = 11.84GCVILIAVSLAAFPFLMFLSS156 pKa = 4.09

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4168

0

4168

1458384

25

3801

349.9

38.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.45 ± 0.042

0.973 ± 0.014

5.186 ± 0.026

5.815 ± 0.042

4.146 ± 0.024

8.221 ± 0.049

1.975 ± 0.021

5.378 ± 0.03

3.877 ± 0.034

9.981 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.015

3.202 ± 0.031

5.356 ± 0.029

3.369 ± 0.022

6.407 ± 0.036

6.487 ± 0.035

5.681 ± 0.038

7.129 ± 0.034

1.621 ± 0.017

2.676 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski