Amino acid dipepetide frequency for Nitrosomonas sp. Nm84

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.307AlaAla: 9.307 ± 0.129
0.995AlaCys: 0.995 ± 0.038
4.8AlaAsp: 4.8 ± 0.091
5.304AlaGlu: 5.304 ± 0.079
3.28AlaPhe: 3.28 ± 0.076
6.841AlaGly: 6.841 ± 0.121
2.029AlaHis: 2.029 ± 0.049
6.494AlaIle: 6.494 ± 0.079
4.123AlaLys: 4.123 ± 0.079
9.626AlaLeu: 9.626 ± 0.14
2.376AlaMet: 2.376 ± 0.054
3.57AlaAsn: 3.57 ± 0.073
3.013AlaPro: 3.013 ± 0.053
3.932AlaGln: 3.932 ± 0.069
4.526AlaArg: 4.526 ± 0.069
5.227AlaSer: 5.227 ± 0.089
4.829AlaThr: 4.829 ± 0.089
5.971AlaVal: 5.971 ± 0.085
1.086AlaTrp: 1.086 ± 0.038
2.425AlaTyr: 2.425 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.831CysAla: 0.831 ± 0.028
0.166CysCys: 0.166 ± 0.014
0.51CysAsp: 0.51 ± 0.024
0.486CysGlu: 0.486 ± 0.024
0.393CysPhe: 0.393 ± 0.021
0.863CysGly: 0.863 ± 0.033
0.355CysHis: 0.355 ± 0.023
0.601CysIle: 0.601 ± 0.027
0.383CysLys: 0.383 ± 0.022
0.926CysLeu: 0.926 ± 0.029
0.218CysMet: 0.218 ± 0.014
0.36CysAsn: 0.36 ± 0.021
0.413CysPro: 0.413 ± 0.022
0.363CysGln: 0.363 ± 0.019
0.499CysArg: 0.499 ± 0.021
0.586CysSer: 0.586 ± 0.028
0.481CysThr: 0.481 ± 0.025
0.649CysVal: 0.649 ± 0.027
0.117CysTrp: 0.117 ± 0.012
0.32CysTyr: 0.32 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
4.876AspAla: 4.876 ± 0.083
0.497AspCys: 0.497 ± 0.025
3.119AspAsp: 3.119 ± 0.079
3.265AspGlu: 3.265 ± 0.063
2.433AspPhe: 2.433 ± 0.052
4.356AspGly: 4.356 ± 0.167
1.315AspHis: 1.315 ± 0.04
3.804AspIle: 3.804 ± 0.069
2.658AspLys: 2.658 ± 0.059
5.514AspLeu: 5.514 ± 0.1
1.31AspMet: 1.31 ± 0.036
2.251AspAsn: 2.251 ± 0.054
2.493AspPro: 2.493 ± 0.054
2.152AspGln: 2.152 ± 0.047
2.782AspArg: 2.782 ± 0.051
3.292AspSer: 3.292 ± 0.068
3.063AspThr: 3.063 ± 0.088
3.545AspVal: 3.545 ± 0.081
0.812AspTrp: 0.812 ± 0.031
1.928AspTyr: 1.928 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
4.994GluAla: 4.994 ± 0.084
0.433GluCys: 0.433 ± 0.021
2.395GluAsp: 2.395 ± 0.059
3.098GluGlu: 3.098 ± 0.069
2.198GluPhe: 2.198 ± 0.05
2.949GluGly: 2.949 ± 0.059
1.431GluHis: 1.431 ± 0.038
4.781GluIle: 4.781 ± 0.093
3.572GluLys: 3.572 ± 0.065
6.004GluLeu: 6.004 ± 0.095
1.493GluMet: 1.493 ± 0.04
2.677GluAsn: 2.677 ± 0.057
1.832GluPro: 1.832 ± 0.048
2.959GluGln: 2.959 ± 0.066
3.444GluArg: 3.444 ± 0.072
3.564GluSer: 3.564 ± 0.062
3.293GluThr: 3.293 ± 0.063
3.452GluVal: 3.452 ± 0.07
0.755GluTrp: 0.755 ± 0.027
1.529GluTyr: 1.529 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.387PheAla: 3.387 ± 0.059
0.465PheCys: 0.465 ± 0.022
2.736PheAsp: 2.736 ± 0.069
2.271PheGlu: 2.271 ± 0.051
1.744PhePhe: 1.744 ± 0.05
3.103PheGly: 3.103 ± 0.089
0.854PheHis: 0.854 ± 0.034
2.537PheIle: 2.537 ± 0.053
1.686PheLys: 1.686 ± 0.042
3.621PheLeu: 3.621 ± 0.074
0.991PheMet: 0.991 ± 0.034
1.806PheAsn: 1.806 ± 0.054
1.617PhePro: 1.617 ± 0.035
1.33PheGln: 1.33 ± 0.038
1.968PheArg: 1.968 ± 0.052
3.123PheSer: 3.123 ± 0.067
2.192PheThr: 2.192 ± 0.049
2.607PheVal: 2.607 ± 0.062
0.501PheTrp: 0.501 ± 0.024
1.303PheTyr: 1.303 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
5.822GlyAla: 5.822 ± 0.122
0.822GlyCys: 0.822 ± 0.034
4.072GlyAsp: 4.072 ± 0.144
3.762GlyGlu: 3.762 ± 0.067
3.54GlyPhe: 3.54 ± 0.135
5.338GlyGly: 5.338 ± 0.143
1.774GlyHis: 1.774 ± 0.045
5.254GlyIle: 5.254 ± 0.084
4.097GlyLys: 4.097 ± 0.066
6.69GlyLeu: 6.69 ± 0.1
1.895GlyMet: 1.895 ± 0.046
3.397GlyAsn: 3.397 ± 0.113
1.696GlyPro: 1.696 ± 0.054
2.659GlyGln: 2.659 ± 0.06
3.53GlyArg: 3.53 ± 0.063
4.437GlySer: 4.437 ± 0.109
3.914GlyThr: 3.914 ± 0.107
4.771GlyVal: 4.771 ± 0.089
0.97GlyTrp: 0.97 ± 0.036
2.463GlyTyr: 2.463 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
2.282HisAla: 2.282 ± 0.053
0.304HisCys: 0.304 ± 0.018
1.373HisAsp: 1.373 ± 0.041
1.318HisGlu: 1.318 ± 0.038
1.049HisPhe: 1.049 ± 0.031
1.781HisGly: 1.781 ± 0.05
0.802HisHis: 0.802 ± 0.036
1.559HisIle: 1.559 ± 0.037
0.936HisLys: 0.936 ± 0.033
2.463HisLeu: 2.463 ± 0.051
0.537HisMet: 0.537 ± 0.024
0.944HisAsn: 0.944 ± 0.035
1.336HisPro: 1.336 ± 0.04
1.144HisGln: 1.144 ± 0.035
1.273HisArg: 1.273 ± 0.041
1.458HisSer: 1.458 ± 0.04
1.268HisThr: 1.268 ± 0.034
1.519HisVal: 1.519 ± 0.036
0.367HisTrp: 0.367 ± 0.019
0.845HisTyr: 0.845 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.996IleAla: 6.996 ± 0.082
0.657IleCys: 0.657 ± 0.027
4.23IleAsp: 4.23 ± 0.067
4.491IleGlu: 4.491 ± 0.08
2.611IlePhe: 2.611 ± 0.06
4.887IleGly: 4.887 ± 0.068
1.674IleHis: 1.674 ± 0.045
4.27IleIle: 4.27 ± 0.072
3.494IleLys: 3.494 ± 0.066
6.202IleLeu: 6.202 ± 0.093
1.453IleMet: 1.453 ± 0.04
3.42IleAsn: 3.42 ± 0.057
3.219IlePro: 3.219 ± 0.062
2.666IleGln: 2.666 ± 0.061
3.482IleArg: 3.482 ± 0.061
4.527IleSer: 4.527 ± 0.074
4.24IleThr: 4.24 ± 0.093
4.48IleVal: 4.48 ± 0.07
0.707IleTrp: 0.707 ± 0.027
1.805IleTyr: 1.805 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
3.983LysAla: 3.983 ± 0.084
0.345LysCys: 0.345 ± 0.021
2.383LysAsp: 2.383 ± 0.058
2.914LysGlu: 2.914 ± 0.066
1.484LysPhe: 1.484 ± 0.043
2.606LysGly: 2.606 ± 0.053
1.154LysHis: 1.154 ± 0.037
3.739LysIle: 3.739 ± 0.078
2.945LysLys: 2.945 ± 0.066
5.004LysLeu: 5.004 ± 0.074
1.286LysMet: 1.286 ± 0.034
2.426LysAsn: 2.426 ± 0.065
2.31LysPro: 2.31 ± 0.054
2.518LysGln: 2.518 ± 0.063
2.676LysArg: 2.676 ± 0.056
2.921LysSer: 2.921 ± 0.069
2.847LysThr: 2.847 ± 0.056
2.806LysVal: 2.806 ± 0.055
0.502LysTrp: 0.502 ± 0.025
1.19LysTyr: 1.19 ± 0.038
0.0LysXaa: 0.0 ± 0.0
Leu
9.492LeuAla: 9.492 ± 0.109
1.0LeuCys: 1.0 ± 0.035
5.968LeuAsp: 5.968 ± 0.127
5.648LeuGlu: 5.648 ± 0.098
3.877LeuPhe: 3.877 ± 0.074
6.722LeuGly: 6.722 ± 0.093
2.397LeuHis: 2.397 ± 0.05
6.99LeuIle: 6.99 ± 0.107
4.862LeuLys: 4.862 ± 0.088
10.995LeuLeu: 10.995 ± 0.162
2.479LeuMet: 2.479 ± 0.053
4.399LeuAsn: 4.399 ± 0.07
4.996LeuPro: 4.996 ± 0.084
4.398LeuGln: 4.398 ± 0.069
5.46LeuArg: 5.46 ± 0.087
7.45LeuSer: 7.45 ± 0.095
6.243LeuThr: 6.243 ± 0.102
6.308LeuVal: 6.308 ± 0.082
1.121LeuTrp: 1.121 ± 0.043
2.479LeuTyr: 2.479 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
2.123MetAla: 2.123 ± 0.051
0.17MetCys: 0.17 ± 0.012
1.199MetAsp: 1.199 ± 0.041
1.259MetGlu: 1.259 ± 0.04
0.781MetPhe: 0.781 ± 0.026
1.587MetGly: 1.587 ± 0.044
0.664MetHis: 0.664 ± 0.028
1.628MetIle: 1.628 ± 0.044
1.338MetLys: 1.338 ± 0.043
2.736MetLeu: 2.736 ± 0.065
0.702MetMet: 0.702 ± 0.029
1.232MetAsn: 1.232 ± 0.04
1.211MetPro: 1.211 ± 0.036
1.264MetGln: 1.264 ± 0.036
1.431MetArg: 1.431 ± 0.042
1.575MetSer: 1.575 ± 0.036
1.432MetThr: 1.432 ± 0.035
1.53MetVal: 1.53 ± 0.045
0.183MetTrp: 0.183 ± 0.013
0.451MetTyr: 0.451 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.647AsnAla: 3.647 ± 0.069
0.377AsnCys: 0.377 ± 0.02
2.617AsnAsp: 2.617 ± 0.094
2.221AsnGlu: 2.221 ± 0.049
1.686AsnPhe: 1.686 ± 0.049
3.43AsnGly: 3.43 ± 0.149
1.101AsnHis: 1.101 ± 0.031
2.911AsnIle: 2.911 ± 0.057
2.096AsnLys: 2.096 ± 0.051
4.435AsnLeu: 4.435 ± 0.082
0.937AsnMet: 0.937 ± 0.03
2.161AsnAsn: 2.161 ± 0.06
2.433AsnPro: 2.433 ± 0.057
2.053AsnGln: 2.053 ± 0.051
2.258AsnArg: 2.258 ± 0.05
2.648AsnSer: 2.648 ± 0.061
2.501AsnThr: 2.501 ± 0.058
2.631AsnVal: 2.631 ± 0.054
0.621AsnTrp: 0.621 ± 0.028
1.283AsnTyr: 1.283 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
3.634ProAla: 3.634 ± 0.072
0.342ProCys: 0.342 ± 0.019
2.81ProAsp: 2.81 ± 0.048
3.043ProGlu: 3.043 ± 0.062
1.663ProPhe: 1.663 ± 0.044
3.061ProGly: 3.061 ± 0.063
0.979ProHis: 0.979 ± 0.032
2.707ProIle: 2.707 ± 0.06
1.721ProLys: 1.721 ± 0.054
4.009ProLeu: 4.009 ± 0.084
0.951ProMet: 0.951 ± 0.034
1.757ProAsn: 1.757 ± 0.048
1.716ProPro: 1.716 ± 0.055
1.75ProGln: 1.75 ± 0.046
1.728ProArg: 1.728 ± 0.041
2.437ProSer: 2.437 ± 0.054
2.167ProThr: 2.167 ± 0.061
3.337ProVal: 3.337 ± 0.06
0.532ProTrp: 0.532 ± 0.024
1.205ProTyr: 1.205 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
4.171GlnAla: 4.171 ± 0.077
0.36GlnCys: 0.36 ± 0.022
1.954GlnAsp: 1.954 ± 0.042
2.359GlnGlu: 2.359 ± 0.057
1.643GlnPhe: 1.643 ± 0.041
2.677GlnGly: 2.677 ± 0.051
1.243GlnHis: 1.243 ± 0.038
2.988GlnIle: 2.988 ± 0.054
2.198GlnLys: 2.198 ± 0.06
4.795GlnLeu: 4.795 ± 0.083
1.055GlnMet: 1.055 ± 0.032
1.698GlnAsn: 1.698 ± 0.043
1.799GlnPro: 1.799 ± 0.048
2.621GlnGln: 2.621 ± 0.058
2.486GlnArg: 2.486 ± 0.058
2.742GlnSer: 2.742 ± 0.06
2.236GlnThr: 2.236 ± 0.05
2.946GlnVal: 2.946 ± 0.056
0.52GlnTrp: 0.52 ± 0.021
1.268GlnTyr: 1.268 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
4.09ArgAla: 4.09 ± 0.071
0.413ArgCys: 0.413 ± 0.023
2.781ArgAsp: 2.781 ± 0.059
3.265ArgGlu: 3.265 ± 0.067
2.323ArgPhe: 2.323 ± 0.05
2.987ArgGly: 2.987 ± 0.05
1.309ArgHis: 1.309 ± 0.039
4.049ArgIle: 4.049 ± 0.067
2.518ArgLys: 2.518 ± 0.056
5.971ArgLeu: 5.971 ± 0.084
1.439ArgMet: 1.439 ± 0.036
2.419ArgAsn: 2.419 ± 0.052
1.835ArgPro: 1.835 ± 0.051
2.362ArgGln: 2.362 ± 0.056
2.913ArgArg: 2.913 ± 0.07
2.881ArgSer: 2.881 ± 0.043
2.576ArgThr: 2.576 ± 0.052
3.292ArgVal: 3.292 ± 0.064
0.801ArgTrp: 0.801 ± 0.029
1.916ArgTyr: 1.916 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
5.662SerAla: 5.662 ± 0.087
0.622SerCys: 0.622 ± 0.028
3.527SerAsp: 3.527 ± 0.067
3.385SerGlu: 3.385 ± 0.064
2.443SerPhe: 2.443 ± 0.059
5.763SerGly: 5.763 ± 0.136
1.514SerHis: 1.514 ± 0.046
4.255SerIle: 4.255 ± 0.074
2.729SerLys: 2.729 ± 0.059
6.472SerLeu: 6.472 ± 0.1
1.581SerMet: 1.581 ± 0.048
2.929SerAsn: 2.929 ± 0.066
2.497SerPro: 2.497 ± 0.052
2.504SerGln: 2.504 ± 0.054
3.075SerArg: 3.075 ± 0.065
4.482SerSer: 4.482 ± 0.113
3.321SerThr: 3.321 ± 0.059
4.229SerVal: 4.229 ± 0.063
0.704SerTrp: 0.704 ± 0.025
1.862SerTyr: 1.862 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
5.045ThrAla: 5.045 ± 0.093
0.465ThrCys: 0.465 ± 0.026
3.107ThrAsp: 3.107 ± 0.068
3.059ThrGlu: 3.059 ± 0.06
2.063ThrPhe: 2.063 ± 0.055
4.656ThrGly: 4.656 ± 0.112
1.41ThrHis: 1.41 ± 0.043
3.747ThrIle: 3.747 ± 0.074
2.109ThrLys: 2.109 ± 0.055
6.508ThrLeu: 6.508 ± 0.11
1.127ThrMet: 1.127 ± 0.032
2.116ThrAsn: 2.116 ± 0.053
2.795ThrPro: 2.795 ± 0.062
2.46ThrGln: 2.46 ± 0.061
2.729ThrArg: 2.729 ± 0.058
3.25ThrSer: 3.25 ± 0.07
3.209ThrThr: 3.209 ± 0.086
3.976ThrVal: 3.976 ± 0.075
0.678ThrTrp: 0.678 ± 0.03
1.509ThrTyr: 1.509 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
6.09ValAla: 6.09 ± 0.098
0.663ValCys: 0.663 ± 0.029
3.799ValAsp: 3.799 ± 0.064
3.637ValGlu: 3.637 ± 0.066
2.752ValPhe: 2.752 ± 0.065
4.474ValGly: 4.474 ± 0.084
1.351ValHis: 1.351 ± 0.043
4.742ValIle: 4.742 ± 0.071
2.893ValLys: 2.893 ± 0.058
6.6ValLeu: 6.6 ± 0.082
1.795ValMet: 1.795 ± 0.051
2.94ValAsn: 2.94 ± 0.066
2.578ValPro: 2.578 ± 0.054
2.29ValGln: 2.29 ± 0.051
3.242ValArg: 3.242 ± 0.064
4.372ValSer: 4.372 ± 0.072
4.141ValThr: 4.141 ± 0.078
4.794ValVal: 4.794 ± 0.075
0.732ValTrp: 0.732 ± 0.027
1.673ValTyr: 1.673 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
0.797TrpAla: 0.797 ± 0.031
0.118TrpCys: 0.118 ± 0.013
0.563TrpAsp: 0.563 ± 0.025
0.588TrpGlu: 0.588 ± 0.024
0.51TrpPhe: 0.51 ± 0.026
0.706TrpGly: 0.706 ± 0.026
0.398TrpHis: 0.398 ± 0.024
0.867TrpIle: 0.867 ± 0.033
0.59TrpLys: 0.59 ± 0.025
1.657TrpLeu: 1.657 ± 0.047
0.338TrpMet: 0.338 ± 0.021
0.544TrpAsn: 0.544 ± 0.024
0.397TrpPro: 0.397 ± 0.019
0.78TrpGln: 0.78 ± 0.037
0.804TrpArg: 0.804 ± 0.027
0.764TrpSer: 0.764 ± 0.031
0.524TrpThr: 0.524 ± 0.023
0.847TrpVal: 0.847 ± 0.03
0.173TrpTrp: 0.173 ± 0.013
0.346TrpTyr: 0.346 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.524TyrAla: 2.524 ± 0.054
0.305TyrCys: 0.305 ± 0.017
1.524TyrAsp: 1.524 ± 0.039
1.483TyrGlu: 1.483 ± 0.041
1.368TyrPhe: 1.368 ± 0.041
2.116TyrGly: 2.116 ± 0.05
0.803TyrHis: 0.803 ± 0.029
1.588TyrIle: 1.588 ± 0.047
1.091TyrLys: 1.091 ± 0.03
3.055TyrLeu: 3.055 ± 0.058
0.534TyrMet: 0.534 ± 0.022
1.002TyrAsn: 1.002 ± 0.037
1.362TyrPro: 1.362 ± 0.04
1.556TyrGln: 1.556 ± 0.044
1.833TyrArg: 1.833 ± 0.046
1.82TyrSer: 1.82 ± 0.05
1.544TyrThr: 1.544 ± 0.046
1.867TyrVal: 1.867 ± 0.052
0.427TyrTrp: 0.427 ± 0.023
1.03TyrTyr: 1.03 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3167 proteins (995109 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski