Apple chlorotic leaf spot virus (isolate plum P863) (ACLSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Trichovirus; Apple chlorotic leaf spot virus

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P27738|RDRP_ACLSP RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate plum P863) OX=73473 PE=4 SV=1
MM1 pKa = 7.42AAVLNLQLKK10 pKa = 9.92VDD12 pKa = 4.05ASLKK16 pKa = 10.59AFLGAEE22 pKa = 3.85NRR24 pKa = 11.84PLHH27 pKa = 6.12GKK29 pKa = 7.87TGATLEE35 pKa = 4.37QILEE39 pKa = 4.41SIFANIAIQGTSEE52 pKa = 3.77QTEE55 pKa = 4.05FLDD58 pKa = 4.33LVVEE62 pKa = 4.51VKK64 pKa = 10.87SMEE67 pKa = 4.3DD68 pKa = 3.25QSVLGSYY75 pKa = 8.65NLKK78 pKa = 10.49EE79 pKa = 4.07VVNLIKK85 pKa = 10.66AFKK88 pKa = 8.02TTSSDD93 pKa = 3.42PNINKK98 pKa = 6.75MTFRR102 pKa = 11.84QVCEE106 pKa = 3.82AFAPEE111 pKa = 4.08ARR113 pKa = 11.84NGLVKK118 pKa = 10.76LKK120 pKa = 10.82YY121 pKa = 10.13KK122 pKa = 10.81GVFTNLFTTMPEE134 pKa = 4.02VGSKK138 pKa = 10.57YY139 pKa = 9.76PEE141 pKa = 4.09LMFDD145 pKa = 4.06FNKK148 pKa = 9.68GLNMFIMNKK157 pKa = 9.41AQQKK161 pKa = 10.48VITNMNRR168 pKa = 11.84RR169 pKa = 11.84LLQTEE174 pKa = 4.41FAKK177 pKa = 10.82SEE179 pKa = 4.12NEE181 pKa = 3.8AKK183 pKa = 10.48LSSVSTDD190 pKa = 2.99LCII193 pKa = 4.94

Molecular weight: 21.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P27739|MP_ACLSP Putative movement protein OS=Apple chlorotic leaf spot virus (isolate plum P863) OX=73473 PE=3 SV=1
MM1 pKa = 7.06ATMIRR6 pKa = 11.84GHH8 pKa = 6.43RR9 pKa = 11.84LRR11 pKa = 11.84IAEE14 pKa = 4.07GDD16 pKa = 3.53IPIAGVKK23 pKa = 9.81SSRR26 pKa = 11.84IYY28 pKa = 11.17SDD30 pKa = 3.19ISPFKK35 pKa = 10.32KK36 pKa = 10.55ASDD39 pKa = 4.81LMIHH43 pKa = 5.83WNEE46 pKa = 3.84FVFKK50 pKa = 10.88VMPEE54 pKa = 4.42DD55 pKa = 3.29IAGDD59 pKa = 4.15GFRR62 pKa = 11.84LASIPVIPSSEE73 pKa = 4.05VQAVLRR79 pKa = 11.84KK80 pKa = 9.69RR81 pKa = 11.84EE82 pKa = 4.1STNYY86 pKa = 7.76VHH88 pKa = 7.3WGALSISIDD97 pKa = 3.16ALFRR101 pKa = 11.84KK102 pKa = 9.49NAGVSGWCYY111 pKa = 10.45VYY113 pKa = 11.0DD114 pKa = 3.98NRR116 pKa = 11.84WEE118 pKa = 4.18TFEE121 pKa = 4.38QAMLQKK127 pKa = 10.27FHH129 pKa = 7.29FNLDD133 pKa = 3.51SGSATLVTSPNFPVSLDD150 pKa = 3.76DD151 pKa = 4.79PGLSNSISVAVMFEE165 pKa = 3.7NLNFKK170 pKa = 10.62FEE172 pKa = 4.6SYY174 pKa = 9.85PISVRR179 pKa = 11.84VGNMCRR185 pKa = 11.84FFDD188 pKa = 4.15SFLSSVKK195 pKa = 10.83NKK197 pKa = 9.81VDD199 pKa = 4.18SNFLLEE205 pKa = 4.93ASNADD210 pKa = 3.83PLGVGAFGFEE220 pKa = 3.93QDD222 pKa = 4.16DD223 pKa = 3.85QVSEE227 pKa = 4.25LFNYY231 pKa = 8.89IQTVPTQAIKK241 pKa = 10.43FRR243 pKa = 11.84EE244 pKa = 4.01HH245 pKa = 7.31EE246 pKa = 4.1IPKK249 pKa = 10.41GFLGMMGKK257 pKa = 9.94KK258 pKa = 9.57KK259 pKa = 10.19IKK261 pKa = 10.29SFEE264 pKa = 4.01FASGSKK270 pKa = 10.26GMEE273 pKa = 3.17RR274 pKa = 11.84RR275 pKa = 11.84KK276 pKa = 10.01PNRR279 pKa = 11.84GKK281 pKa = 10.62QIDD284 pKa = 3.53RR285 pKa = 11.84SFSQRR290 pKa = 11.84AVPGFRR296 pKa = 11.84SQNEE300 pKa = 4.23KK301 pKa = 10.61VEE303 pKa = 4.16HH304 pKa = 6.17QGLSTDD310 pKa = 3.31SDD312 pKa = 3.95FEE314 pKa = 4.14NFLRR318 pKa = 11.84NKK320 pKa = 9.58RR321 pKa = 11.84GNKK324 pKa = 9.97AGVKK328 pKa = 8.55STASEE333 pKa = 4.33GSSVDD338 pKa = 3.67NISSRR343 pKa = 11.84EE344 pKa = 3.83FQFARR349 pKa = 11.84QNQAKK354 pKa = 9.3EE355 pKa = 4.42DD356 pKa = 3.86GSSSEE361 pKa = 4.17FAAQGGRR368 pKa = 11.84KK369 pKa = 9.19SKK371 pKa = 10.75GISGRR376 pKa = 11.84RR377 pKa = 11.84KK378 pKa = 8.27QTSSWKK384 pKa = 10.28DD385 pKa = 3.04RR386 pKa = 11.84GNPGTDD392 pKa = 2.55TGVHH396 pKa = 5.57LRR398 pKa = 11.84EE399 pKa = 4.5HH400 pKa = 6.98SDD402 pKa = 3.4PGNVRR407 pKa = 11.84ADD409 pKa = 3.64GVSGPSGGSEE419 pKa = 3.8INGGSISPRR428 pKa = 11.84VLQPEE433 pKa = 4.53GSGQLDD439 pKa = 3.41QSFQDD444 pKa = 3.65YY445 pKa = 10.98LFGPEE450 pKa = 4.28HH451 pKa = 5.68QQNDD455 pKa = 3.66IPSGLL460 pKa = 3.64

Molecular weight: 50.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2537

193

1884

845.7

96.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.124 ± 0.786

2.05 ± 0.757

5.558 ± 0.646

7.174 ± 0.518

5.952 ± 0.426

6.543 ± 1.22

2.444 ± 0.592

6.07 ± 0.563

8.08 ± 0.788

8.79 ± 1.39

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.396

4.493 ± 1.032

3.666 ± 0.415

3.193 ± 0.821

5.873 ± 0.866

8.869 ± 1.419

3.508 ± 0.871

5.794 ± 0.434

1.064 ± 0.285

2.917 ± 0.733
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski