Nocardioides daphniae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7U8T4|A0A4P7U8T4_9ACTN SGNH/GDSL hydrolase family protein OS=Nocardioides daphniae OX=402297 GN=E2C04_04000 PE=4 SV=1
MM1 pKa = 7.2LTACGNDD8 pKa = 3.21SDD10 pKa = 4.87GSPEE14 pKa = 3.68GTAVDD19 pKa = 4.0GRR21 pKa = 11.84TASSPGVDD29 pKa = 3.05LGDD32 pKa = 3.49VVEE35 pKa = 4.82YY36 pKa = 11.1DD37 pKa = 3.62DD38 pKa = 6.07LSRR41 pKa = 11.84NHH43 pKa = 5.9VNGTVNYY50 pKa = 7.5PQNPPVGGDD59 pKa = 3.56HH60 pKa = 6.91APVWMNCAAYY70 pKa = 9.66DD71 pKa = 3.92VPVDD75 pKa = 3.49PGMAVHH81 pKa = 6.47SMEE84 pKa = 5.45HH85 pKa = 5.4GAVWLAYY92 pKa = 10.19DD93 pKa = 4.32PGLPSEE99 pKa = 5.33DD100 pKa = 3.0VDD102 pKa = 3.89ALRR105 pKa = 11.84ALTSSNGFVLVSPVEE120 pKa = 4.84DD121 pKa = 3.32MDD123 pKa = 4.67SPVAATAWGRR133 pKa = 11.84QLTQDD138 pKa = 3.49SVDD141 pKa = 3.89LSRR144 pKa = 11.84LSAFVNTFAQGPQTPEE160 pKa = 3.69LGAPCTGGMGG170 pKa = 3.36

Molecular weight: 17.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7UDI2|A0A4P7UDI2_9ACTN Uncharacterized protein OS=Nocardioides daphniae OX=402297 GN=E2C04_09725 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.68KK16 pKa = 9.82HH17 pKa = 5.64RR18 pKa = 11.84KK19 pKa = 8.3LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2853

0

2853

840121

33

1715

294.5

31.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.062 ± 0.065

0.716 ± 0.013

6.219 ± 0.038

5.937 ± 0.049

2.755 ± 0.028

9.001 ± 0.042

2.258 ± 0.023

3.093 ± 0.035

2.208 ± 0.042

10.081 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.901 ± 0.018

1.738 ± 0.023

5.625 ± 0.039

2.797 ± 0.023

7.749 ± 0.053

5.47 ± 0.034

6.218 ± 0.045

9.786 ± 0.051

1.629 ± 0.022

1.756 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski