Amino acid dipepetide frequency for Bacillus phage vB_BboS-125

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.506AlaAla: 12.506 ± 1.176
0.682AlaCys: 0.682 ± 0.254
4.605AlaAsp: 4.605 ± 0.429
7.788AlaGlu: 7.788 ± 0.695
3.013AlaPhe: 3.013 ± 0.476
7.845AlaGly: 7.845 ± 0.702
1.08AlaHis: 1.08 ± 0.254
4.605AlaIle: 4.605 ± 0.542
5.685AlaLys: 5.685 ± 0.737
7.049AlaLeu: 7.049 ± 0.598
1.99AlaMet: 1.99 ± 0.528
3.525AlaAsn: 3.525 ± 0.473
3.013AlaPro: 3.013 ± 0.323
4.605AlaGln: 4.605 ± 0.577
4.832AlaArg: 4.832 ± 0.664
3.525AlaSer: 3.525 ± 0.417
5.287AlaThr: 5.287 ± 0.719
8.072AlaVal: 8.072 ± 0.765
1.307AlaTrp: 1.307 ± 0.28
3.297AlaTyr: 3.297 ± 0.412
0.0AlaXaa: 0.0 ± 0.0
Cys
0.227CysAla: 0.227 ± 0.169
0.057CysCys: 0.057 ± 0.064
0.057CysAsp: 0.057 ± 0.057
0.398CysGlu: 0.398 ± 0.207
0.284CysPhe: 0.284 ± 0.172
0.512CysGly: 0.512 ± 0.23
0.0CysHis: 0.0 ± 0.0
0.341CysIle: 0.341 ± 0.126
0.227CysLys: 0.227 ± 0.152
0.171CysLeu: 0.171 ± 0.104
0.171CysMet: 0.171 ± 0.083
0.455CysAsn: 0.455 ± 0.211
0.341CysPro: 0.341 ± 0.157
0.227CysGln: 0.227 ± 0.104
0.227CysArg: 0.227 ± 0.104
0.284CysSer: 0.284 ± 0.121
0.114CysThr: 0.114 ± 0.075
0.057CysVal: 0.057 ± 0.058
0.114CysTrp: 0.114 ± 0.08
0.568CysTyr: 0.568 ± 0.202
0.0CysXaa: 0.0 ± 0.0
Asp
5.003AspAla: 5.003 ± 0.452
0.341AspCys: 0.341 ± 0.151
3.922AspAsp: 3.922 ± 0.595
4.32AspGlu: 4.32 ± 0.523
2.444AspPhe: 2.444 ± 0.358
3.922AspGly: 3.922 ± 0.418
0.682AspHis: 0.682 ± 0.177
3.183AspIle: 3.183 ± 0.474
4.207AspLys: 4.207 ± 0.665
6.537AspLeu: 6.537 ± 0.626
0.966AspMet: 0.966 ± 0.223
1.762AspAsn: 1.762 ± 0.356
2.444AspPro: 2.444 ± 0.539
2.786AspGln: 2.786 ± 0.375
3.468AspArg: 3.468 ± 0.53
1.933AspSer: 1.933 ± 0.347
3.525AspThr: 3.525 ± 0.44
3.525AspVal: 3.525 ± 0.482
0.91AspTrp: 0.91 ± 0.224
3.183AspTyr: 3.183 ± 0.425
0.0AspXaa: 0.0 ± 0.0
Glu
10.176GluAla: 10.176 ± 0.868
0.171GluCys: 0.171 ± 0.096
1.705GluAsp: 1.705 ± 0.295
8.015GluGlu: 8.015 ± 1.004
2.729GluPhe: 2.729 ± 0.406
6.651GluGly: 6.651 ± 0.724
0.853GluHis: 0.853 ± 0.217
5.912GluIle: 5.912 ± 0.678
5.742GluLys: 5.742 ± 0.633
7.618GluLeu: 7.618 ± 0.698
1.933GluMet: 1.933 ± 0.353
2.956GluAsn: 2.956 ± 0.328
1.99GluPro: 1.99 ± 0.347
3.013GluGln: 3.013 ± 0.4
4.434GluArg: 4.434 ± 0.568
1.876GluSer: 1.876 ± 0.272
4.548GluThr: 4.548 ± 0.516
6.14GluVal: 6.14 ± 0.859
1.08GluTrp: 1.08 ± 0.325
3.183GluTyr: 3.183 ± 0.529
0.0GluXaa: 0.0 ± 0.0
Phe
1.762PheAla: 1.762 ± 0.237
0.171PheCys: 0.171 ± 0.141
2.16PheAsp: 2.16 ± 0.476
2.786PheGlu: 2.786 ± 0.368
1.194PhePhe: 1.194 ± 0.282
2.103PheGly: 2.103 ± 0.292
0.625PheHis: 0.625 ± 0.158
1.99PheIle: 1.99 ± 0.357
2.558PheLys: 2.558 ± 0.419
2.615PheLeu: 2.615 ± 0.43
1.023PheMet: 1.023 ± 0.204
2.103PheAsn: 2.103 ± 0.4
0.853PhePro: 0.853 ± 0.2
1.592PheGln: 1.592 ± 0.391
0.966PheArg: 0.966 ± 0.21
1.194PheSer: 1.194 ± 0.283
2.729PheThr: 2.729 ± 0.482
2.331PheVal: 2.331 ± 0.378
0.341PheTrp: 0.341 ± 0.145
1.137PheTyr: 1.137 ± 0.303
0.0PheXaa: 0.0 ± 0.0
Gly
5.173GlyAla: 5.173 ± 0.679
0.284GlyCys: 0.284 ± 0.184
5.003GlyAsp: 5.003 ± 0.496
5.685GlyGlu: 5.685 ± 0.522
2.729GlyPhe: 2.729 ± 0.408
4.605GlyGly: 4.605 ± 0.487
1.762GlyHis: 1.762 ± 0.344
4.889GlyIle: 4.889 ± 0.867
6.651GlyLys: 6.651 ± 0.533
5.344GlyLeu: 5.344 ± 0.713
1.99GlyMet: 1.99 ± 0.379
3.695GlyAsn: 3.695 ± 0.469
2.274GlyPro: 2.274 ± 0.324
3.468GlyGln: 3.468 ± 0.44
4.605GlyArg: 4.605 ± 0.622
3.979GlySer: 3.979 ± 0.539
5.173GlyThr: 5.173 ± 0.828
5.4GlyVal: 5.4 ± 0.668
1.137GlyTrp: 1.137 ± 0.228
2.786GlyTyr: 2.786 ± 0.464
0.0GlyXaa: 0.0 ± 0.0
His
0.966HisAla: 0.966 ± 0.246
0.114HisCys: 0.114 ± 0.092
0.682HisAsp: 0.682 ± 0.157
0.91HisGlu: 0.91 ± 0.237
0.455HisPhe: 0.455 ± 0.163
0.568HisGly: 0.568 ± 0.184
0.341HisHis: 0.341 ± 0.125
1.08HisIle: 1.08 ± 0.299
1.592HisLys: 1.592 ± 0.349
0.91HisLeu: 0.91 ± 0.266
0.341HisMet: 0.341 ± 0.112
0.796HisAsn: 0.796 ± 0.226
0.853HisPro: 0.853 ± 0.226
0.398HisGln: 0.398 ± 0.154
0.625HisArg: 0.625 ± 0.193
1.08HisSer: 1.08 ± 0.284
1.251HisThr: 1.251 ± 0.263
0.91HisVal: 0.91 ± 0.232
0.227HisTrp: 0.227 ± 0.103
0.568HisTyr: 0.568 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
3.354IleAla: 3.354 ± 0.364
0.114IleCys: 0.114 ± 0.074
3.922IleAsp: 3.922 ± 0.462
4.207IleGlu: 4.207 ± 0.487
1.876IlePhe: 1.876 ± 0.367
3.752IleGly: 3.752 ± 0.631
0.739IleHis: 0.739 ± 0.23
2.842IleIle: 2.842 ± 0.597
4.377IleLys: 4.377 ± 0.57
4.605IleLeu: 4.605 ± 0.442
1.194IleMet: 1.194 ± 0.336
2.274IleAsn: 2.274 ± 0.296
2.615IlePro: 2.615 ± 0.363
2.842IleGln: 2.842 ± 0.519
3.183IleArg: 3.183 ± 0.412
2.899IleSer: 2.899 ± 0.481
4.377IleThr: 4.377 ± 0.6
3.638IleVal: 3.638 ± 0.552
0.625IleTrp: 0.625 ± 0.318
1.762IleTyr: 1.762 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
8.3LysAla: 8.3 ± 0.804
0.455LysCys: 0.455 ± 0.164
5.287LysAsp: 5.287 ± 0.605
7.049LysGlu: 7.049 ± 0.824
1.478LysPhe: 1.478 ± 0.29
6.196LysGly: 6.196 ± 0.567
1.194LysHis: 1.194 ± 0.263
2.956LysIle: 2.956 ± 0.433
5.798LysLys: 5.798 ± 0.609
5.912LysLeu: 5.912 ± 0.684
1.705LysMet: 1.705 ± 0.312
2.842LysAsn: 2.842 ± 0.392
2.729LysPro: 2.729 ± 0.469
4.093LysGln: 4.093 ± 0.472
4.32LysArg: 4.32 ± 0.562
1.99LysSer: 1.99 ± 0.34
3.866LysThr: 3.866 ± 0.53
4.548LysVal: 4.548 ± 0.468
0.739LysTrp: 0.739 ± 0.235
3.07LysTyr: 3.07 ± 0.488
0.0LysXaa: 0.0 ± 0.0
Leu
8.413LeuAla: 8.413 ± 0.588
0.398LeuCys: 0.398 ± 0.167
6.083LeuAsp: 6.083 ± 0.572
7.788LeuGlu: 7.788 ± 0.83
3.013LeuPhe: 3.013 ± 0.32
7.106LeuGly: 7.106 ± 0.742
0.91LeuHis: 0.91 ± 0.24
4.093LeuIle: 4.093 ± 0.42
6.026LeuLys: 6.026 ± 0.597
6.992LeuLeu: 6.992 ± 0.756
1.876LeuMet: 1.876 ± 0.363
3.411LeuAsn: 3.411 ± 0.451
3.411LeuPro: 3.411 ± 0.505
4.207LeuGln: 4.207 ± 0.485
4.605LeuArg: 4.605 ± 0.427
3.809LeuSer: 3.809 ± 0.41
4.718LeuThr: 4.718 ± 0.517
5.457LeuVal: 5.457 ± 0.72
0.796LeuTrp: 0.796 ± 0.226
2.331LeuTyr: 2.331 ± 0.368
0.0LeuXaa: 0.0 ± 0.0
Met
2.388MetAla: 2.388 ± 0.364
0.114MetCys: 0.114 ± 0.089
2.217MetAsp: 2.217 ± 0.362
2.047MetGlu: 2.047 ± 0.412
0.398MetPhe: 0.398 ± 0.141
2.047MetGly: 2.047 ± 0.57
0.227MetHis: 0.227 ± 0.111
1.137MetIle: 1.137 ± 0.328
1.649MetLys: 1.649 ± 0.329
1.99MetLeu: 1.99 ± 0.451
0.227MetMet: 0.227 ± 0.109
1.194MetAsn: 1.194 ± 0.266
0.853MetPro: 0.853 ± 0.196
0.625MetGln: 0.625 ± 0.166
1.364MetArg: 1.364 ± 0.289
0.966MetSer: 0.966 ± 0.341
0.853MetThr: 0.853 ± 0.275
0.966MetVal: 0.966 ± 0.225
0.0MetTrp: 0.0 ± 0.0
0.568MetTyr: 0.568 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.468AsnAla: 3.468 ± 0.404
0.341AsnCys: 0.341 ± 0.176
2.331AsnAsp: 2.331 ± 0.345
2.615AsnGlu: 2.615 ± 0.289
1.535AsnPhe: 1.535 ± 0.251
3.581AsnGly: 3.581 ± 0.584
0.398AsnHis: 0.398 ± 0.16
1.933AsnIle: 1.933 ± 0.349
3.183AsnLys: 3.183 ± 0.524
2.899AsnLeu: 2.899 ± 0.513
1.194AsnMet: 1.194 ± 0.226
1.592AsnAsn: 1.592 ± 0.324
2.615AsnPro: 2.615 ± 0.453
1.762AsnGln: 1.762 ± 0.329
1.876AsnArg: 1.876 ± 0.358
2.388AsnSer: 2.388 ± 0.404
2.16AsnThr: 2.16 ± 0.384
3.127AsnVal: 3.127 ± 0.476
0.455AsnTrp: 0.455 ± 0.196
1.819AsnTyr: 1.819 ± 0.378
0.0AsnXaa: 0.0 ± 0.0
Pro
2.899ProAla: 2.899 ± 0.417
0.114ProCys: 0.114 ± 0.081
2.388ProAsp: 2.388 ± 0.44
3.581ProGlu: 3.581 ± 0.338
1.251ProPhe: 1.251 ± 0.315
3.638ProGly: 3.638 ± 0.579
0.796ProHis: 0.796 ± 0.216
1.876ProIle: 1.876 ± 0.297
2.615ProLys: 2.615 ± 0.411
3.07ProLeu: 3.07 ± 0.379
0.398ProMet: 0.398 ± 0.154
1.876ProAsn: 1.876 ± 0.354
1.535ProPro: 1.535 ± 0.306
1.705ProGln: 1.705 ± 0.322
1.251ProArg: 1.251 ± 0.303
1.819ProSer: 1.819 ± 0.309
2.217ProThr: 2.217 ± 0.418
2.729ProVal: 2.729 ± 0.414
0.455ProTrp: 0.455 ± 0.167
1.364ProTyr: 1.364 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
5.798GlnAla: 5.798 ± 0.592
0.057GlnCys: 0.057 ± 0.063
2.331GlnAsp: 2.331 ± 0.345
3.525GlnGlu: 3.525 ± 0.481
0.682GlnPhe: 0.682 ± 0.2
2.501GlnGly: 2.501 ± 0.413
1.023GlnHis: 1.023 ± 0.269
2.103GlnIle: 2.103 ± 0.374
4.264GlnLys: 4.264 ± 0.72
6.253GlnLeu: 6.253 ± 0.68
0.853GlnMet: 0.853 ± 0.252
1.762GlnAsn: 1.762 ± 0.327
1.99GlnPro: 1.99 ± 0.338
3.354GlnGln: 3.354 ± 0.525
2.956GlnArg: 2.956 ± 0.395
1.137GlnSer: 1.137 ± 0.292
1.649GlnThr: 1.649 ± 0.326
3.013GlnVal: 3.013 ± 0.413
0.341GlnTrp: 0.341 ± 0.144
1.592GlnTyr: 1.592 ± 0.359
0.0GlnXaa: 0.0 ± 0.0
Arg
3.581ArgAla: 3.581 ± 0.401
0.398ArgCys: 0.398 ± 0.157
2.103ArgAsp: 2.103 ± 0.411
3.468ArgGlu: 3.468 ± 0.444
1.933ArgPhe: 1.933 ± 0.376
3.127ArgGly: 3.127 ± 0.406
0.512ArgHis: 0.512 ± 0.19
4.264ArgIle: 4.264 ± 0.499
4.377ArgLys: 4.377 ± 0.548
5.059ArgLeu: 5.059 ± 0.507
1.251ArgMet: 1.251 ± 0.252
2.331ArgAsn: 2.331 ± 0.368
1.876ArgPro: 1.876 ± 0.376
2.388ArgGln: 2.388 ± 0.375
3.127ArgArg: 3.127 ± 0.416
2.672ArgSer: 2.672 ± 0.348
3.127ArgThr: 3.127 ± 0.469
3.354ArgVal: 3.354 ± 0.462
1.194ArgTrp: 1.194 ± 0.291
2.615ArgTyr: 2.615 ± 0.428
0.0ArgXaa: 0.0 ± 0.0
Ser
2.331SerAla: 2.331 ± 0.589
0.398SerCys: 0.398 ± 0.154
2.842SerAsp: 2.842 ± 0.431
2.047SerGlu: 2.047 ± 0.296
2.16SerPhe: 2.16 ± 0.324
4.093SerGly: 4.093 ± 0.628
0.625SerHis: 0.625 ± 0.247
2.729SerIle: 2.729 ± 0.534
3.354SerLys: 3.354 ± 0.43
3.411SerLeu: 3.411 ± 0.457
1.307SerMet: 1.307 ± 0.46
1.819SerAsn: 1.819 ± 0.503
1.023SerPro: 1.023 ± 0.252
2.103SerGln: 2.103 ± 0.356
2.274SerArg: 2.274 ± 0.401
2.444SerSer: 2.444 ± 0.457
2.274SerThr: 2.274 ± 0.361
2.615SerVal: 2.615 ± 0.312
0.739SerTrp: 0.739 ± 0.155
2.103SerTyr: 2.103 ± 0.366
0.0SerXaa: 0.0 ± 0.0
Thr
7.674ThrAla: 7.674 ± 0.834
0.057ThrCys: 0.057 ± 0.058
3.354ThrAsp: 3.354 ± 0.425
3.866ThrGlu: 3.866 ± 0.495
1.876ThrPhe: 1.876 ± 0.384
6.14ThrGly: 6.14 ± 0.733
0.739ThrHis: 0.739 ± 0.239
3.183ThrIle: 3.183 ± 0.421
3.638ThrLys: 3.638 ± 0.494
4.832ThrLeu: 4.832 ± 0.483
0.796ThrMet: 0.796 ± 0.228
1.99ThrAsn: 1.99 ± 0.325
2.786ThrPro: 2.786 ± 0.36
1.99ThrGln: 1.99 ± 0.336
2.786ThrArg: 2.786 ± 0.35
2.558ThrSer: 2.558 ± 0.363
3.581ThrThr: 3.581 ± 0.762
3.695ThrVal: 3.695 ± 0.621
1.307ThrTrp: 1.307 ± 0.301
1.99ThrTyr: 1.99 ± 0.354
0.0ThrXaa: 0.0 ± 0.0
Val
6.765ValAla: 6.765 ± 0.664
0.398ValCys: 0.398 ± 0.14
4.605ValAsp: 4.605 ± 0.801
6.367ValGlu: 6.367 ± 0.5
1.876ValPhe: 1.876 ± 0.382
3.866ValGly: 3.866 ± 0.454
0.91ValHis: 0.91 ± 0.188
3.809ValIle: 3.809 ± 0.536
5.287ValLys: 5.287 ± 0.656
5.003ValLeu: 5.003 ± 0.577
1.819ValMet: 1.819 ± 0.309
2.388ValAsn: 2.388 ± 0.394
3.127ValPro: 3.127 ± 0.436
2.956ValGln: 2.956 ± 0.448
3.07ValArg: 3.07 ± 0.387
3.411ValSer: 3.411 ± 0.432
4.491ValThr: 4.491 ± 0.493
4.15ValVal: 4.15 ± 0.504
0.853ValTrp: 0.853 ± 0.286
2.842ValTyr: 2.842 ± 0.482
0.0ValXaa: 0.0 ± 0.0
Trp
1.023TrpAla: 1.023 ± 0.281
0.057TrpCys: 0.057 ± 0.051
0.682TrpAsp: 0.682 ± 0.181
1.023TrpGlu: 1.023 ± 0.243
0.227TrpPhe: 0.227 ± 0.133
1.535TrpGly: 1.535 ± 0.293
0.341TrpHis: 0.341 ± 0.129
0.625TrpIle: 0.625 ± 0.218
1.08TrpLys: 1.08 ± 0.295
1.307TrpLeu: 1.307 ± 0.19
0.0TrpMet: 0.0 ± 0.0
0.625TrpAsn: 0.625 ± 0.194
0.341TrpPro: 0.341 ± 0.114
0.91TrpGln: 0.91 ± 0.21
0.455TrpArg: 0.455 ± 0.133
0.398TrpSer: 0.398 ± 0.239
0.796TrpThr: 0.796 ± 0.208
1.08TrpVal: 1.08 ± 0.255
0.057TrpTrp: 0.057 ± 0.056
0.796TrpTyr: 0.796 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.558TyrAla: 2.558 ± 0.345
0.114TyrCys: 0.114 ± 0.069
2.786TyrAsp: 2.786 ± 0.443
3.24TyrGlu: 3.24 ± 0.405
1.08TyrPhe: 1.08 ± 0.277
2.842TyrGly: 2.842 ± 0.353
0.91TyrHis: 0.91 ± 0.242
1.649TyrIle: 1.649 ± 0.263
2.217TyrLys: 2.217 ± 0.377
3.866TyrLeu: 3.866 ± 0.479
0.739TyrMet: 0.739 ± 0.226
1.876TyrAsn: 1.876 ± 0.35
0.91TyrPro: 0.91 ± 0.248
2.16TyrGln: 2.16 ± 0.374
2.331TyrArg: 2.331 ± 0.392
2.331TyrSer: 2.331 ± 0.422
2.103TyrThr: 2.103 ± 0.334
3.183TyrVal: 3.183 ± 0.433
0.682TyrTrp: 0.682 ± 0.184
1.307TyrTyr: 1.307 ± 0.309
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (17592 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski