Amino acid dipepetide frequency for Elusimicrobium minutum (strain Pei191)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.869AlaAla: 8.869 ± 0.172
1.286AlaCys: 1.286 ± 0.08
4.283AlaAsp: 4.283 ± 0.096
5.257AlaGlu: 5.257 ± 0.124
3.687AlaPhe: 3.687 ± 0.104
6.187AlaGly: 6.187 ± 0.142
1.189AlaHis: 1.189 ± 0.048
4.848AlaIle: 4.848 ± 0.113
7.479AlaLys: 7.479 ± 0.142
8.49AlaLeu: 8.49 ± 0.151
1.868AlaMet: 1.868 ± 0.059
3.098AlaAsn: 3.098 ± 0.086
2.777AlaPro: 2.777 ± 0.11
3.209AlaGln: 3.209 ± 0.076
3.061AlaArg: 3.061 ± 0.08
4.755AlaSer: 4.755 ± 0.115
3.12AlaThr: 3.12 ± 0.088
7.003AlaVal: 7.003 ± 0.141
0.658AlaTrp: 0.658 ± 0.038
2.658AlaTyr: 2.658 ± 0.074
0.0AlaXaa: 0.0 ± 0.0
Cys
1.114CysAla: 1.114 ± 0.057
0.334CysCys: 0.334 ± 0.054
0.555CysAsp: 0.555 ± 0.039
0.606CysGlu: 0.606 ± 0.038
0.557CysPhe: 0.557 ± 0.037
1.193CysGly: 1.193 ± 0.065
0.19CysHis: 0.19 ± 0.02
0.679CysIle: 0.679 ± 0.037
0.912CysLys: 0.912 ± 0.058
0.938CysLeu: 0.938 ± 0.043
0.245CysMet: 0.245 ± 0.027
0.476CysAsn: 0.476 ± 0.038
0.762CysPro: 0.762 ± 0.087
0.296CysGln: 0.296 ± 0.028
0.417CysArg: 0.417 ± 0.03
0.701CysSer: 0.701 ± 0.054
0.549CysThr: 0.549 ± 0.041
0.877CysVal: 0.877 ± 0.055
0.097CysTrp: 0.097 ± 0.016
0.401CysTyr: 0.401 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
3.58AspAla: 3.58 ± 0.087
0.502AspCys: 0.502 ± 0.035
1.789AspAsp: 1.789 ± 0.064
2.777AspGlu: 2.777 ± 0.089
3.071AspPhe: 3.071 ± 0.082
3.397AspGly: 3.397 ± 0.091
0.634AspHis: 0.634 ± 0.037
5.127AspIle: 5.127 ± 0.11
3.961AspLys: 3.961 ± 0.094
4.686AspLeu: 4.686 ± 0.12
1.274AspMet: 1.274 ± 0.051
2.628AspAsn: 2.628 ± 0.08
1.827AspPro: 1.827 ± 0.069
0.825AspGln: 0.825 ± 0.038
1.73AspArg: 1.73 ± 0.057
2.844AspSer: 2.844 ± 0.086
2.322AspThr: 2.322 ± 0.073
3.225AspVal: 3.225 ± 0.079
0.502AspTrp: 0.502 ± 0.026
2.109AspTyr: 2.109 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
4.957GluAla: 4.957 ± 0.114
0.573GluCys: 0.573 ± 0.032
3.472GluAsp: 3.472 ± 0.094
5.217GluGlu: 5.217 ± 0.13
2.561GluPhe: 2.561 ± 0.07
4.133GluGly: 4.133 ± 0.107
1.112GluHis: 1.112 ± 0.056
5.553GluIle: 5.553 ± 0.113
6.086GluLys: 6.086 ± 0.134
5.358GluLeu: 5.358 ± 0.113
1.558GluMet: 1.558 ± 0.058
3.835GluAsn: 3.835 ± 0.107
1.797GluPro: 1.797 ± 0.062
2.111GluGln: 2.111 ± 0.063
2.873GluArg: 2.873 ± 0.083
2.998GluSer: 2.998 ± 0.08
3.379GluThr: 3.379 ± 0.08
4.11GluVal: 4.11 ± 0.096
0.456GluTrp: 0.456 ± 0.03
2.271GluTyr: 2.271 ± 0.083
0.0GluXaa: 0.0 ± 0.0
Phe
4.042PheAla: 4.042 ± 0.084
0.648PheCys: 0.648 ± 0.041
2.733PheAsp: 2.733 ± 0.073
2.891PheGlu: 2.891 ± 0.088
2.852PhePhe: 2.852 ± 0.105
3.316PheGly: 3.316 ± 0.089
0.636PheHis: 0.636 ± 0.034
4.042PheIle: 4.042 ± 0.112
4.068PheLys: 4.068 ± 0.093
4.787PheLeu: 4.787 ± 0.136
1.276PheMet: 1.276 ± 0.048
2.638PheAsn: 2.638 ± 0.083
1.473PhePro: 1.473 ± 0.055
0.893PheGln: 0.893 ± 0.04
1.351PheArg: 1.351 ± 0.052
3.393PheSer: 3.393 ± 0.087
2.982PheThr: 2.982 ± 0.076
3.047PheVal: 3.047 ± 0.086
0.551PheTrp: 0.551 ± 0.04
1.884PheTyr: 1.884 ± 0.069
0.0PheXaa: 0.0 ± 0.0
Gly
5.83GlyAla: 5.83 ± 0.122
0.818GlyCys: 0.818 ± 0.05
3.057GlyAsp: 3.057 ± 0.076
3.847GlyGlu: 3.847 ± 0.084
3.73GlyPhe: 3.73 ± 0.09
5.606GlyGly: 5.606 ± 0.143
1.161GlyHis: 1.161 ± 0.054
5.275GlyIle: 5.275 ± 0.128
6.049GlyLys: 6.049 ± 0.125
6.078GlyLeu: 6.078 ± 0.132
1.767GlyMet: 1.767 ± 0.064
2.952GlyAsn: 2.952 ± 0.091
1.805GlyPro: 1.805 ± 0.062
2.036GlyGln: 2.036 ± 0.081
2.883GlyArg: 2.883 ± 0.081
4.117GlySer: 4.117 ± 0.111
3.669GlyThr: 3.669 ± 0.104
5.393GlyVal: 5.393 ± 0.114
0.687GlyTrp: 0.687 ± 0.032
2.682GlyTyr: 2.682 ± 0.078
0.0GlyXaa: 0.0 ± 0.0
His
1.151HisAla: 1.151 ± 0.051
0.188HisCys: 0.188 ± 0.024
0.66HisAsp: 0.66 ± 0.04
0.816HisGlu: 0.816 ± 0.042
0.818HisPhe: 0.818 ± 0.04
1.185HisGly: 1.185 ± 0.055
0.284HisHis: 0.284 ± 0.022
1.337HisIle: 1.337 ± 0.057
1.116HisLys: 1.116 ± 0.045
1.341HisLeu: 1.341 ± 0.053
0.377HisMet: 0.377 ± 0.029
0.766HisAsn: 0.766 ± 0.044
0.75HisPro: 0.75 ± 0.041
0.314HisGln: 0.314 ± 0.026
0.565HisArg: 0.565 ± 0.038
0.944HisSer: 0.944 ± 0.046
0.857HisThr: 0.857 ± 0.038
0.825HisVal: 0.825 ± 0.036
0.13HisTrp: 0.13 ± 0.017
0.6HisTyr: 0.6 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.088IleAla: 6.088 ± 0.121
0.912IleCys: 0.912 ± 0.046
3.711IleAsp: 3.711 ± 0.097
4.884IleGlu: 4.884 ± 0.114
3.932IlePhe: 3.932 ± 0.111
4.645IleGly: 4.645 ± 0.111
0.893IleHis: 0.893 ± 0.044
6.027IleIle: 6.027 ± 0.138
7.097IleLys: 7.097 ± 0.133
7.218IleLeu: 7.218 ± 0.142
1.758IleMet: 1.758 ± 0.058
3.934IleAsn: 3.934 ± 0.097
2.891IlePro: 2.891 ± 0.078
1.408IleGln: 1.408 ± 0.057
2.401IleArg: 2.401 ± 0.076
5.294IleSer: 5.294 ± 0.11
4.554IleThr: 4.554 ± 0.094
4.686IleVal: 4.686 ± 0.098
0.567IleTrp: 0.567 ± 0.037
2.53IleTyr: 2.53 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
6.726LysAla: 6.726 ± 0.146
0.703LysCys: 0.703 ± 0.049
5.115LysAsp: 5.115 ± 0.105
7.771LysGlu: 7.771 ± 0.178
3.093LysPhe: 3.093 ± 0.084
5.344LysGly: 5.344 ± 0.116
1.442LysHis: 1.442 ± 0.057
7.038LysIle: 7.038 ± 0.142
8.898LysLys: 8.898 ± 0.183
6.163LysLeu: 6.163 ± 0.116
2.609LysMet: 2.609 ± 0.07
5.818LysAsn: 5.818 ± 0.12
2.654LysPro: 2.654 ± 0.093
2.887LysGln: 2.887 ± 0.078
3.294LysArg: 3.294 ± 0.084
4.185LysSer: 4.185 ± 0.091
6.124LysThr: 6.124 ± 0.134
4.753LysVal: 4.753 ± 0.101
0.537LysTrp: 0.537 ± 0.035
3.254LysTyr: 3.254 ± 0.079
0.0LysXaa: 0.0 ± 0.0
Leu
7.7LeuAla: 7.7 ± 0.139
1.084LeuCys: 1.084 ± 0.053
4.445LeuAsp: 4.445 ± 0.111
5.8LeuGlu: 5.8 ± 0.121
4.424LeuPhe: 4.424 ± 0.138
6.44LeuGly: 6.44 ± 0.116
1.313LeuHis: 1.313 ± 0.05
6.558LeuIle: 6.558 ± 0.13
8.357LeuLys: 8.357 ± 0.141
7.743LeuLeu: 7.743 ± 0.167
2.107LeuMet: 2.107 ± 0.07
4.651LeuAsn: 4.651 ± 0.113
3.892LeuPro: 3.892 ± 0.082
2.522LeuGln: 2.522 ± 0.073
3.391LeuArg: 3.391 ± 0.115
6.527LeuSer: 6.527 ± 0.127
5.314LeuThr: 5.314 ± 0.111
5.158LeuVal: 5.158 ± 0.117
0.703LeuTrp: 0.703 ± 0.04
2.717LeuTyr: 2.717 ± 0.082
0.0LeuXaa: 0.0 ± 0.0
Met
2.253MetAla: 2.253 ± 0.074
0.229MetCys: 0.229 ± 0.022
1.163MetAsp: 1.163 ± 0.049
1.416MetGlu: 1.416 ± 0.059
1.076MetPhe: 1.076 ± 0.047
1.852MetGly: 1.852 ± 0.067
0.423MetHis: 0.423 ± 0.029
1.408MetIle: 1.408 ± 0.058
2.103MetLys: 2.103 ± 0.058
2.449MetLeu: 2.449 ± 0.071
0.492MetMet: 0.492 ± 0.036
0.926MetAsn: 0.926 ± 0.04
1.325MetPro: 1.325 ± 0.044
0.94MetGln: 0.94 ± 0.042
1.124MetArg: 1.124 ± 0.043
1.625MetSer: 1.625 ± 0.068
1.211MetThr: 1.211 ± 0.047
1.609MetVal: 1.609 ± 0.059
0.19MetTrp: 0.19 ± 0.021
0.648MetTyr: 0.648 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.882AsnAla: 3.882 ± 0.094
0.571AsnCys: 0.571 ± 0.043
1.921AsnAsp: 1.921 ± 0.063
2.573AsnGlu: 2.573 ± 0.077
2.613AsnPhe: 2.613 ± 0.074
3.116AsnGly: 3.116 ± 0.117
0.636AsnHis: 0.636 ± 0.038
4.878AsnIle: 4.878 ± 0.12
4.631AsnLys: 4.631 ± 0.104
4.483AsnLeu: 4.483 ± 0.118
1.463AsnMet: 1.463 ± 0.058
2.7AsnAsn: 2.7 ± 0.101
2.557AsnPro: 2.557 ± 0.078
1.07AsnGln: 1.07 ± 0.049
1.694AsnArg: 1.694 ± 0.066
2.964AsnSer: 2.964 ± 0.089
2.775AsnThr: 2.775 ± 0.079
2.972AsnVal: 2.972 ± 0.089
0.502AsnTrp: 0.502 ± 0.033
1.846AsnTyr: 1.846 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
3.389ProAla: 3.389 ± 0.131
0.367ProCys: 0.367 ± 0.034
1.694ProAsp: 1.694 ± 0.058
2.656ProGlu: 2.656 ± 0.083
2.054ProPhe: 2.054 ± 0.067
2.409ProGly: 2.409 ± 0.081
0.64ProHis: 0.64 ± 0.032
2.024ProIle: 2.024 ± 0.06
2.915ProLys: 2.915 ± 0.083
3.385ProLeu: 3.385 ± 0.096
0.786ProMet: 0.786 ± 0.04
1.424ProAsn: 1.424 ± 0.049
1.155ProPro: 1.155 ± 0.065
1.641ProGln: 1.641 ± 0.064
1.151ProArg: 1.151 ± 0.049
2.69ProSer: 2.69 ± 0.082
1.722ProThr: 1.722 ± 0.078
3.217ProVal: 3.217 ± 0.088
0.273ProTrp: 0.273 ± 0.023
1.446ProTyr: 1.446 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
2.48GlnAla: 2.48 ± 0.084
0.259GlnCys: 0.259 ± 0.025
1.453GlnAsp: 1.453 ± 0.055
1.945GlnGlu: 1.945 ± 0.071
0.985GlnPhe: 0.985 ± 0.041
1.868GlnGly: 1.868 ± 0.056
0.46GlnHis: 0.46 ± 0.033
2.184GlnIle: 2.184 ± 0.065
2.832GlnLys: 2.832 ± 0.091
2.206GlnLeu: 2.206 ± 0.062
0.906GlnMet: 0.906 ± 0.044
1.801GlnAsn: 1.801 ± 0.069
0.934GlnPro: 0.934 ± 0.057
1.003GlnGln: 1.003 ± 0.059
1.23GlnArg: 1.23 ± 0.049
1.566GlnSer: 1.566 ± 0.056
1.817GlnThr: 1.817 ± 0.063
1.625GlnVal: 1.625 ± 0.058
0.19GlnTrp: 0.19 ± 0.022
1.088GlnTyr: 1.088 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
3.061ArgAla: 3.061 ± 0.082
0.346ArgCys: 0.346 ± 0.029
1.807ArgAsp: 1.807 ± 0.073
2.68ArgGlu: 2.68 ± 0.08
1.797ArgPhe: 1.797 ± 0.064
2.431ArgGly: 2.431 ± 0.074
0.695ArgHis: 0.695 ± 0.041
2.881ArgIle: 2.881 ± 0.075
2.968ArgLys: 2.968 ± 0.089
3.254ArgLeu: 3.254 ± 0.089
0.978ArgMet: 0.978 ± 0.046
1.716ArgAsn: 1.716 ± 0.063
1.343ArgPro: 1.343 ± 0.062
1.28ArgGln: 1.28 ± 0.05
1.655ArgArg: 1.655 ± 0.065
2.038ArgSer: 2.038 ± 0.065
1.931ArgThr: 1.931 ± 0.071
2.48ArgVal: 2.48 ± 0.071
0.312ArgTrp: 0.312 ± 0.025
1.426ArgTyr: 1.426 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
5.581SerAla: 5.581 ± 0.11
0.729SerCys: 0.729 ± 0.057
3.008SerAsp: 3.008 ± 0.081
3.529SerGlu: 3.529 ± 0.082
3.596SerPhe: 3.596 ± 0.09
4.905SerGly: 4.905 ± 0.121
0.829SerHis: 0.829 ± 0.04
3.746SerIle: 3.746 ± 0.093
5.146SerLys: 5.146 ± 0.105
6.055SerLeu: 6.055 ± 0.117
1.459SerMet: 1.459 ± 0.054
2.676SerAsn: 2.676 ± 0.084
2.139SerPro: 2.139 ± 0.068
1.763SerGln: 1.763 ± 0.058
2.166SerArg: 2.166 ± 0.075
4.185SerSer: 4.185 ± 0.104
2.688SerThr: 2.688 ± 0.085
4.53SerVal: 4.53 ± 0.102
0.515SerTrp: 0.515 ± 0.029
2.239SerTyr: 2.239 ± 0.074
0.0SerXaa: 0.0 ± 0.0
Thr
5.148ThrAla: 5.148 ± 0.106
0.677ThrCys: 0.677 ± 0.075
2.678ThrAsp: 2.678 ± 0.081
3.108ThrGlu: 3.108 ± 0.082
2.34ThrPhe: 2.34 ± 0.069
4.57ThrGly: 4.57 ± 0.125
0.81ThrHis: 0.81 ± 0.04
3.529ThrIle: 3.529 ± 0.088
4.062ThrLys: 4.062 ± 0.097
5.318ThrLeu: 5.318 ± 0.111
0.997ThrMet: 0.997 ± 0.041
2.046ThrAsn: 2.046 ± 0.071
2.8ThrPro: 2.8 ± 0.084
1.673ThrGln: 1.673 ± 0.058
1.835ThrArg: 1.835 ± 0.064
3.223ThrSer: 3.223 ± 0.101
2.634ThrThr: 2.634 ± 0.088
4.05ThrVal: 4.05 ± 0.103
0.405ThrTrp: 0.405 ± 0.027
1.542ThrTyr: 1.542 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
4.453ValAla: 4.453 ± 0.104
1.072ValCys: 1.072 ± 0.055
3.004ValAsp: 3.004 ± 0.078
4.169ValGlu: 4.169 ± 0.092
3.884ValPhe: 3.884 ± 0.106
3.691ValGly: 3.691 ± 0.101
0.93ValHis: 0.93 ± 0.049
5.223ValIle: 5.223 ± 0.116
6.061ValLys: 6.061 ± 0.113
6.894ValLeu: 6.894 ± 0.135
1.517ValMet: 1.517 ± 0.06
3.288ValAsn: 3.288 ± 0.1
2.451ValPro: 2.451 ± 0.066
1.629ValGln: 1.629 ± 0.066
2.542ValArg: 2.542 ± 0.075
4.506ValSer: 4.506 ± 0.103
3.424ValThr: 3.424 ± 0.099
4.503ValVal: 4.503 ± 0.111
0.685ValTrp: 0.685 ± 0.041
2.435ValTyr: 2.435 ± 0.075
0.0ValXaa: 0.0 ± 0.0
Trp
0.808TrpAla: 0.808 ± 0.043
0.111TrpCys: 0.111 ± 0.015
0.484TrpAsp: 0.484 ± 0.033
0.478TrpGlu: 0.478 ± 0.034
0.409TrpPhe: 0.409 ± 0.028
0.614TrpGly: 0.614 ± 0.04
0.186TrpHis: 0.186 ± 0.022
0.579TrpIle: 0.579 ± 0.034
0.502TrpLys: 0.502 ± 0.03
0.792TrpLeu: 0.792 ± 0.046
0.221TrpMet: 0.221 ± 0.02
0.413TrpAsn: 0.413 ± 0.029
0.249TrpPro: 0.249 ± 0.027
0.338TrpGln: 0.338 ± 0.023
0.365TrpArg: 0.365 ± 0.031
0.434TrpSer: 0.434 ± 0.033
0.476TrpThr: 0.476 ± 0.032
0.539TrpVal: 0.539 ± 0.031
0.111TrpTrp: 0.111 ± 0.015
0.271TrpTyr: 0.271 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.672TyrAla: 2.672 ± 0.083
0.488TyrCys: 0.488 ± 0.034
1.779TyrAsp: 1.779 ± 0.062
1.989TyrGlu: 1.989 ± 0.064
2.024TyrPhe: 2.024 ± 0.071
2.538TyrGly: 2.538 ± 0.082
0.559TyrHis: 0.559 ± 0.032
2.496TyrIle: 2.496 ± 0.072
3.168TyrLys: 3.168 ± 0.086
3.207TyrLeu: 3.207 ± 0.089
0.802TyrMet: 0.802 ± 0.039
2.06TyrAsn: 2.06 ± 0.069
1.459TyrPro: 1.459 ± 0.061
0.993TyrGln: 0.993 ± 0.046
1.351TyrArg: 1.351 ± 0.055
2.494TyrSer: 2.494 ± 0.081
1.896TyrThr: 1.896 ± 0.069
1.76TyrVal: 1.76 ± 0.064
0.31TyrTrp: 0.31 ± 0.027
1.467TyrTyr: 1.467 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1528 proteins (493618 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski