Amino acid dipepetide frequency for Actinomyces sp. S4-C9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.622AlaAla: 12.622 ± 0.196
0.776AlaCys: 0.776 ± 0.048
6.268AlaAsp: 6.268 ± 0.112
6.258AlaGlu: 6.258 ± 0.142
3.459AlaPhe: 3.459 ± 0.087
9.819AlaGly: 9.819 ± 0.161
2.088AlaHis: 2.088 ± 0.069
5.835AlaIle: 5.835 ± 0.136
4.67AlaLys: 4.67 ± 0.11
11.588AlaLeu: 11.588 ± 0.178
2.823AlaMet: 2.823 ± 0.089
3.191AlaAsn: 3.191 ± 0.076
4.483AlaPro: 4.483 ± 0.109
4.495AlaGln: 4.495 ± 0.105
7.237AlaArg: 7.237 ± 0.133
6.467AlaSer: 6.467 ± 0.116
6.181AlaThr: 6.181 ± 0.117
8.764AlaVal: 8.764 ± 0.151
1.466AlaTrp: 1.466 ± 0.06
2.214AlaTyr: 2.214 ± 0.067
0.0AlaXaa: 0.0 ± 0.0
Cys
0.652CysAla: 0.652 ± 0.035
0.065CysCys: 0.065 ± 0.012
0.366CysAsp: 0.366 ± 0.029
0.508CysGlu: 0.508 ± 0.036
0.199CysPhe: 0.199 ± 0.024
0.737CysGly: 0.737 ± 0.045
0.144CysHis: 0.144 ± 0.016
0.278CysIle: 0.278 ± 0.026
0.16CysLys: 0.16 ± 0.015
0.538CysLeu: 0.538 ± 0.037
0.14CysMet: 0.14 ± 0.017
0.165CysAsn: 0.165 ± 0.019
0.398CysPro: 0.398 ± 0.037
0.183CysGln: 0.183 ± 0.018
0.315CysArg: 0.315 ± 0.026
0.382CysSer: 0.382 ± 0.027
0.382CysThr: 0.382 ± 0.029
0.475CysVal: 0.475 ± 0.032
0.051CysTrp: 0.051 ± 0.01
0.144CysTyr: 0.144 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
6.24AspAla: 6.24 ± 0.121
0.319AspCys: 0.319 ± 0.026
3.203AspAsp: 3.203 ± 0.094
4.726AspGlu: 4.726 ± 0.114
2.066AspPhe: 2.066 ± 0.068
4.783AspGly: 4.783 ± 0.105
1.158AspHis: 1.158 ± 0.047
2.852AspIle: 2.852 ± 0.077
1.852AspLys: 1.852 ± 0.068
6.297AspLeu: 6.297 ± 0.115
1.28AspMet: 1.28 ± 0.049
1.339AspAsn: 1.339 ± 0.056
3.5AspPro: 3.5 ± 0.093
1.932AspGln: 1.932 ± 0.063
3.278AspArg: 3.278 ± 0.084
3.11AspSer: 3.11 ± 0.085
2.71AspThr: 2.71 ± 0.069
5.104AspVal: 5.104 ± 0.101
0.855AspTrp: 0.855 ± 0.048
1.485AspTyr: 1.485 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
7.806GluAla: 7.806 ± 0.151
0.335GluCys: 0.335 ± 0.026
4.028GluAsp: 4.028 ± 0.095
4.728GluGlu: 4.728 ± 0.121
1.968GluPhe: 1.968 ± 0.062
4.944GluGly: 4.944 ± 0.125
1.399GluHis: 1.399 ± 0.057
3.599GluIle: 3.599 ± 0.088
2.423GluLys: 2.423 ± 0.082
6.638GluLeu: 6.638 ± 0.132
1.466GluMet: 1.466 ± 0.055
2.181GluAsn: 2.181 ± 0.075
2.766GluPro: 2.766 ± 0.079
2.661GluGln: 2.661 ± 0.077
4.481GluArg: 4.481 ± 0.105
3.459GluSer: 3.459 ± 0.087
3.229GluThr: 3.229 ± 0.08
5.565GluVal: 5.565 ± 0.127
0.908GluTrp: 0.908 ± 0.055
1.631GluTyr: 1.631 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.79PheAla: 3.79 ± 0.099
0.227PheCys: 0.227 ± 0.02
2.458PheAsp: 2.458 ± 0.074
2.395PheGlu: 2.395 ± 0.082
1.251PhePhe: 1.251 ± 0.055
3.199PheGly: 3.199 ± 0.072
0.634PheHis: 0.634 ± 0.041
1.609PheIle: 1.609 ± 0.065
1.105PheLys: 1.105 ± 0.053
2.937PheLeu: 2.937 ± 0.098
0.684PheMet: 0.684 ± 0.042
1.052PheAsn: 1.052 ± 0.051
1.44PhePro: 1.44 ± 0.051
0.853PheGln: 0.853 ± 0.038
1.473PheArg: 1.473 ± 0.046
2.167PheSer: 2.167 ± 0.072
2.127PheThr: 2.127 ± 0.065
2.752PheVal: 2.752 ± 0.082
0.451PheTrp: 0.451 ± 0.026
0.847PheTyr: 0.847 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
8.677GlyAla: 8.677 ± 0.143
0.514GlyCys: 0.514 ± 0.035
4.137GlyAsp: 4.137 ± 0.091
5.403GlyGlu: 5.403 ± 0.106
3.116GlyPhe: 3.116 ± 0.072
6.406GlyGly: 6.406 ± 0.131
1.613GlyHis: 1.613 ± 0.061
4.749GlyIle: 4.749 ± 0.107
3.615GlyLys: 3.615 ± 0.086
7.926GlyLeu: 7.926 ± 0.133
2.22GlyMet: 2.22 ± 0.066
2.344GlyAsn: 2.344 ± 0.074
3.104GlyPro: 3.104 ± 0.079
2.754GlyGln: 2.754 ± 0.074
5.112GlyArg: 5.112 ± 0.106
4.928GlySer: 4.928 ± 0.095
4.678GlyThr: 4.678 ± 0.096
7.048GlyVal: 7.048 ± 0.141
1.28GlyTrp: 1.28 ± 0.05
2.11GlyTyr: 2.11 ± 0.068
0.0GlyXaa: 0.0 ± 0.0
His
1.932HisAla: 1.932 ± 0.061
0.138HisCys: 0.138 ± 0.019
1.156HisAsp: 1.156 ± 0.047
1.337HisGlu: 1.337 ± 0.06
0.642HisPhe: 0.642 ± 0.042
1.525HisGly: 1.525 ± 0.067
0.463HisHis: 0.463 ± 0.029
1.009HisIle: 1.009 ± 0.045
0.595HisLys: 0.595 ± 0.034
2.001HisLeu: 2.001 ± 0.067
0.435HisMet: 0.435 ± 0.031
0.522HisAsn: 0.522 ± 0.031
1.217HisPro: 1.217 ± 0.051
0.607HisGln: 0.607 ± 0.035
1.271HisArg: 1.271 ± 0.054
1.095HisSer: 1.095 ± 0.051
1.034HisThr: 1.034 ± 0.046
1.785HisVal: 1.785 ± 0.061
0.26HisTrp: 0.26 ± 0.024
0.506HisTyr: 0.506 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.636IleAla: 6.636 ± 0.142
0.414IleCys: 0.414 ± 0.03
3.662IleAsp: 3.662 ± 0.095
3.731IleGlu: 3.731 ± 0.096
1.749IlePhe: 1.749 ± 0.06
4.773IleGly: 4.773 ± 0.109
0.904IleHis: 0.904 ± 0.037
2.789IleIle: 2.789 ± 0.091
1.631IleLys: 1.631 ± 0.059
4.702IleLeu: 4.702 ± 0.11
1.137IleMet: 1.137 ± 0.055
1.623IleAsn: 1.623 ± 0.061
2.734IlePro: 2.734 ± 0.073
1.29IleGln: 1.29 ± 0.049
2.898IleArg: 2.898 ± 0.082
3.396IleSer: 3.396 ± 0.077
3.095IleThr: 3.095 ± 0.078
4.767IleVal: 4.767 ± 0.106
0.632IleTrp: 0.632 ± 0.035
1.243IleTyr: 1.243 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
3.949LysAla: 3.949 ± 0.1
0.189LysCys: 0.189 ± 0.019
2.1LysAsp: 2.1 ± 0.073
2.305LysGlu: 2.305 ± 0.072
1.125LysPhe: 1.125 ± 0.044
2.366LysGly: 2.366 ± 0.08
0.774LysHis: 0.774 ± 0.041
2.086LysIle: 2.086 ± 0.064
1.838LysLys: 1.838 ± 0.081
3.347LysLeu: 3.347 ± 0.086
1.062LysMet: 1.062 ± 0.044
1.391LysAsn: 1.391 ± 0.06
1.893LysPro: 1.893 ± 0.074
1.357LysGln: 1.357 ± 0.051
2.661LysArg: 2.661 ± 0.073
2.271LysSer: 2.271 ± 0.067
2.275LysThr: 2.275 ± 0.078
3.006LysVal: 3.006 ± 0.086
0.528LysTrp: 0.528 ± 0.031
1.003LysTyr: 1.003 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
11.701LeuAla: 11.701 ± 0.177
0.536LeuCys: 0.536 ± 0.036
6.039LeuAsp: 6.039 ± 0.134
6.297LeuGlu: 6.297 ± 0.136
3.039LeuPhe: 3.039 ± 0.085
8.161LeuGly: 8.161 ± 0.151
1.791LeuHis: 1.791 ± 0.062
5.043LeuIle: 5.043 ± 0.122
3.209LeuLys: 3.209 ± 0.084
8.9LeuLeu: 8.9 ± 0.168
2.283LeuMet: 2.283 ± 0.076
2.671LeuAsn: 2.671 ± 0.065
4.905LeuPro: 4.905 ± 0.104
2.953LeuGln: 2.953 ± 0.07
5.848LeuArg: 5.848 ± 0.135
5.84LeuSer: 5.84 ± 0.103
5.785LeuThr: 5.785 ± 0.12
8.452LeuVal: 8.452 ± 0.142
1.111LeuTrp: 1.111 ± 0.046
1.952LeuTyr: 1.952 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.626MetAla: 2.626 ± 0.079
0.171MetCys: 0.171 ± 0.019
1.217MetAsp: 1.217 ± 0.042
1.286MetGlu: 1.286 ± 0.048
0.709MetPhe: 0.709 ± 0.042
1.889MetGly: 1.889 ± 0.065
0.487MetHis: 0.487 ± 0.031
1.308MetIle: 1.308 ± 0.055
0.969MetLys: 0.969 ± 0.046
2.295MetLeu: 2.295 ± 0.069
0.561MetMet: 0.561 ± 0.035
0.841MetAsn: 0.841 ± 0.045
1.259MetPro: 1.259 ± 0.055
0.76MetGln: 0.76 ± 0.039
1.759MetArg: 1.759 ± 0.063
1.879MetSer: 1.879 ± 0.059
1.556MetThr: 1.556 ± 0.059
1.936MetVal: 1.936 ± 0.061
0.315MetTrp: 0.315 ± 0.025
0.5MetTyr: 0.5 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.006AsnAla: 3.006 ± 0.078
0.209AsnCys: 0.209 ± 0.018
1.513AsnAsp: 1.513 ± 0.064
2.045AsnGlu: 2.045 ± 0.066
0.898AsnPhe: 0.898 ± 0.039
2.053AsnGly: 2.053 ± 0.067
0.64AsnHis: 0.64 ± 0.033
1.468AsnIle: 1.468 ± 0.056
0.938AsnLys: 0.938 ± 0.043
2.984AsnLeu: 2.984 ± 0.073
0.778AsnMet: 0.778 ± 0.038
0.77AsnAsn: 0.77 ± 0.043
1.999AsnPro: 1.999 ± 0.058
1.07AsnGln: 1.07 ± 0.048
1.824AsnArg: 1.824 ± 0.061
1.552AsnSer: 1.552 ± 0.056
1.702AsnThr: 1.702 ± 0.058
2.462AsnVal: 2.462 ± 0.066
0.489AsnTrp: 0.489 ± 0.029
0.808AsnTyr: 0.808 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
5.301ProAla: 5.301 ± 0.12
0.25ProCys: 0.25 ± 0.021
2.965ProAsp: 2.965 ± 0.092
3.451ProGlu: 3.451 ± 0.088
1.582ProPhe: 1.582 ± 0.058
4.137ProGly: 4.137 ± 0.099
1.091ProHis: 1.091 ± 0.042
2.344ProIle: 2.344 ± 0.061
1.783ProLys: 1.783 ± 0.064
4.213ProLeu: 4.213 ± 0.097
0.977ProMet: 0.977 ± 0.041
1.566ProAsn: 1.566 ± 0.053
1.57ProPro: 1.57 ± 0.063
1.814ProGln: 1.814 ± 0.062
2.634ProArg: 2.634 ± 0.09
2.988ProSer: 2.988 ± 0.08
2.768ProThr: 2.768 ± 0.083
4.213ProVal: 4.213 ± 0.101
0.685ProTrp: 0.685 ± 0.039
1.206ProTyr: 1.206 ± 0.055
0.0ProXaa: 0.0 ± 0.0
Gln
3.894GlnAla: 3.894 ± 0.091
0.167GlnCys: 0.167 ± 0.02
1.621GlnAsp: 1.621 ± 0.054
1.909GlnGlu: 1.909 ± 0.065
1.022GlnPhe: 1.022 ± 0.05
2.242GlnGly: 2.242 ± 0.063
0.63GlnHis: 0.63 ± 0.039
2.344GlnIle: 2.344 ± 0.066
1.302GlnLys: 1.302 ± 0.053
3.483GlnLeu: 3.483 ± 0.098
1.115GlnMet: 1.115 ± 0.05
0.959GlnAsn: 0.959 ± 0.045
1.558GlnPro: 1.558 ± 0.065
1.304GlnGln: 1.304 ± 0.055
2.338GlnArg: 2.338 ± 0.074
1.674GlnSer: 1.674 ± 0.053
1.854GlnThr: 1.854 ± 0.059
3.102GlnVal: 3.102 ± 0.08
0.579GlnTrp: 0.579 ± 0.04
0.77GlnTyr: 0.77 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
6.684ArgAla: 6.684 ± 0.134
0.372ArgCys: 0.372 ± 0.03
3.504ArgAsp: 3.504 ± 0.085
4.808ArgGlu: 4.808 ± 0.111
2.11ArgPhe: 2.11 ± 0.063
4.668ArgGly: 4.668 ± 0.097
1.213ArgHis: 1.213 ± 0.051
3.471ArgIle: 3.471 ± 0.089
2.508ArgLys: 2.508 ± 0.084
5.718ArgLeu: 5.718 ± 0.12
1.631ArgMet: 1.631 ± 0.065
1.623ArgAsn: 1.623 ± 0.06
2.84ArgPro: 2.84 ± 0.083
2.141ArgGln: 2.141 ± 0.075
4.698ArgArg: 4.698 ± 0.126
3.595ArgSer: 3.595 ± 0.099
3.339ArgThr: 3.339 ± 0.093
4.999ArgVal: 4.999 ± 0.103
0.831ArgTrp: 0.831 ± 0.033
1.548ArgTyr: 1.548 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
6.463SerAla: 6.463 ± 0.12
0.364SerCys: 0.364 ± 0.029
3.353SerAsp: 3.353 ± 0.09
3.754SerGlu: 3.754 ± 0.089
2.157SerPhe: 2.157 ± 0.062
5.484SerGly: 5.484 ± 0.096
1.001SerHis: 1.001 ± 0.049
3.252SerIle: 3.252 ± 0.08
2.378SerLys: 2.378 ± 0.07
5.642SerLeu: 5.642 ± 0.109
1.527SerMet: 1.527 ± 0.054
1.773SerAsn: 1.773 ± 0.059
2.638SerPro: 2.638 ± 0.067
2.139SerGln: 2.139 ± 0.07
3.628SerArg: 3.628 ± 0.084
4.056SerSer: 4.056 ± 0.107
3.555SerThr: 3.555 ± 0.093
4.718SerVal: 4.718 ± 0.094
0.936SerTrp: 0.936 ± 0.047
1.401SerTyr: 1.401 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
5.549ThrAla: 5.549 ± 0.11
0.39ThrCys: 0.39 ± 0.027
3.02ThrAsp: 3.02 ± 0.081
3.142ThrGlu: 3.142 ± 0.083
2.015ThrPhe: 2.015 ± 0.074
4.997ThrGly: 4.997 ± 0.114
1.211ThrHis: 1.211 ± 0.049
3.156ThrIle: 3.156 ± 0.079
2.11ThrLys: 2.11 ± 0.065
5.521ThrLeu: 5.521 ± 0.116
1.261ThrMet: 1.261 ± 0.046
1.643ThrAsn: 1.643 ± 0.059
3.359ThrPro: 3.359 ± 0.083
1.785ThrGln: 1.785 ± 0.07
3.225ThrArg: 3.225 ± 0.087
3.467ThrSer: 3.467 ± 0.081
3.39ThrThr: 3.39 ± 0.108
5.023ThrVal: 5.023 ± 0.109
0.886ThrTrp: 0.886 ± 0.049
1.332ThrTyr: 1.332 ± 0.053
0.0ThrXaa: 0.0 ± 0.0
Val
9.581ValAla: 9.581 ± 0.155
0.581ValCys: 0.581 ± 0.035
5.265ValAsp: 5.265 ± 0.128
5.764ValGlu: 5.764 ± 0.115
2.88ValPhe: 2.88 ± 0.077
6.617ValGly: 6.617 ± 0.136
1.519ValHis: 1.519 ± 0.064
4.7ValIle: 4.7 ± 0.095
3.16ValLys: 3.16 ± 0.087
8.023ValLeu: 8.023 ± 0.151
1.966ValMet: 1.966 ± 0.071
2.311ValAsn: 2.311 ± 0.072
4.052ValPro: 4.052 ± 0.089
2.405ValGln: 2.405 ± 0.068
5.127ValArg: 5.127 ± 0.102
5.628ValSer: 5.628 ± 0.106
4.775ValThr: 4.775 ± 0.1
7.828ValVal: 7.828 ± 0.15
1.058ValTrp: 1.058 ± 0.045
1.655ValTyr: 1.655 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
1.306TrpAla: 1.306 ± 0.056
0.091TrpCys: 0.091 ± 0.013
0.827TrpAsp: 0.827 ± 0.04
0.756TrpGlu: 0.756 ± 0.036
0.559TrpPhe: 0.559 ± 0.03
0.875TrpGly: 0.875 ± 0.04
0.329TrpHis: 0.329 ± 0.023
0.894TrpIle: 0.894 ± 0.041
0.53TrpLys: 0.53 ± 0.035
1.418TrpLeu: 1.418 ± 0.062
0.447TrpMet: 0.447 ± 0.038
0.557TrpAsn: 0.557 ± 0.031
0.605TrpPro: 0.605 ± 0.036
0.581TrpGln: 0.581 ± 0.036
0.884TrpArg: 0.884 ± 0.045
0.792TrpSer: 0.792 ± 0.046
0.792TrpThr: 0.792 ± 0.042
1.105TrpVal: 1.105 ± 0.053
0.29TrpTrp: 0.29 ± 0.025
0.347TrpTyr: 0.347 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.289TyrAla: 2.289 ± 0.064
0.173TyrCys: 0.173 ± 0.018
1.389TyrAsp: 1.389 ± 0.081
1.609TyrGlu: 1.609 ± 0.065
0.904TyrPhe: 0.904 ± 0.047
2.062TyrGly: 2.062 ± 0.069
0.441TyrHis: 0.441 ± 0.028
1.02TyrIle: 1.02 ± 0.054
0.752TyrLys: 0.752 ± 0.041
2.344TyrLeu: 2.344 ± 0.066
0.532TyrMet: 0.532 ± 0.032
0.684TyrAsn: 0.684 ± 0.043
1.217TyrPro: 1.217 ± 0.056
0.784TyrGln: 0.784 ± 0.045
1.613TyrArg: 1.613 ± 0.064
1.469TyrSer: 1.469 ± 0.051
1.253TyrThr: 1.253 ± 0.054
1.789TyrVal: 1.789 ± 0.064
0.374TyrTrp: 0.374 ± 0.028
0.632TyrTyr: 0.632 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1447 proteins (507667 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski