Chiloscyllium punctatum (Brownbanded bambooshark) (Hemiscyllium punctatum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Chondrichthyes; Elasmobranchii; Selachii; Galeomorphii; Galeoidea; Orectolobiformes; Hemiscylliidae; Chiloscyllium

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401TEQ2|A0A401TEQ2_CHIPU Uncharacterized protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0025122 PE=4 SV=1
LL1 pKa = 8.01VSGSDD6 pKa = 3.54DD7 pKa = 3.69HH8 pKa = 7.82SVSGSDD14 pKa = 2.9NHH16 pKa = 6.13TVSGSDD22 pKa = 3.57DD23 pKa = 3.75QPVSGSDD30 pKa = 3.32DD31 pKa = 3.7HH32 pKa = 7.33AVSGSDD38 pKa = 3.08VHH40 pKa = 6.1TVSGSDD46 pKa = 3.27DD47 pKa = 3.74HH48 pKa = 7.38AVSEE52 pKa = 4.58SDD54 pKa = 3.68VPPVSGSYY62 pKa = 10.36DD63 pKa = 3.56HH64 pKa = 7.03LVSGCDD70 pKa = 3.37DD71 pKa = 4.04HH72 pKa = 7.07PVSVSDD78 pKa = 4.0DD79 pKa = 3.79CLVSGNDD86 pKa = 3.22DD87 pKa = 3.72HH88 pKa = 7.96AGSRR92 pKa = 11.84SDD94 pKa = 3.2VHH96 pKa = 7.17AVSGSDD102 pKa = 3.54DD103 pKa = 3.66QSVSGSDD110 pKa = 3.65DD111 pKa = 3.75DD112 pKa = 4.96AVSGSDD118 pKa = 3.1VHH120 pKa = 7.3AVSGSDD126 pKa = 3.81DD127 pKa = 3.95EE128 pKa = 4.74PVSGSDD134 pKa = 3.28DD135 pKa = 3.65HH136 pKa = 7.23AVSGISEE143 pKa = 4.42SDD145 pKa = 3.6DD146 pKa = 3.55QPVSGGDD153 pKa = 3.55DD154 pKa = 3.47NAVSGIDD161 pKa = 3.68DD162 pKa = 3.89QPVSGSDD169 pKa = 3.58DD170 pKa = 3.7QPDD173 pKa = 3.98SVSDD177 pKa = 3.83DD178 pKa = 3.88SLVSGSDD185 pKa = 3.44DD186 pKa = 3.17QAVFGNDD193 pKa = 2.85DD194 pKa = 3.8HH195 pKa = 7.26AVSGSDD201 pKa = 3.27DD202 pKa = 3.78HH203 pKa = 7.4AVSGSDD209 pKa = 3.38DD210 pKa = 3.83HH211 pKa = 7.95ADD213 pKa = 3.72SGSDD217 pKa = 3.34DD218 pKa = 3.88HH219 pKa = 7.42AVSGNDD225 pKa = 3.23DD226 pKa = 4.15HH227 pKa = 7.22PVSGCDD233 pKa = 3.19DD234 pKa = 3.98HH235 pKa = 8.87SVSEE239 pKa = 4.47SDD241 pKa = 5.01DD242 pKa = 4.15LPLSGSDD249 pKa = 3.49DD250 pKa = 4.07HH251 pKa = 6.99PVSGSDD257 pKa = 3.37DD258 pKa = 3.48HH259 pKa = 7.74SLSGRR264 pKa = 11.84HH265 pKa = 5.7DD266 pKa = 3.6HH267 pKa = 6.08TVSGSGDD274 pKa = 3.63HH275 pKa = 6.65PVPGSDD281 pKa = 3.34DD282 pKa = 4.11HH283 pKa = 7.27PVLGCDD289 pKa = 3.42DD290 pKa = 4.4HH291 pKa = 7.09PVPEE295 pKa = 5.41SDD297 pKa = 3.49DD298 pKa = 3.89HH299 pKa = 6.85SVSVRR304 pKa = 11.84DD305 pKa = 4.02DD306 pKa = 3.69QPVSGSDD313 pKa = 3.52DD314 pKa = 3.73QPVSGSDD321 pKa = 2.78HH322 pKa = 6.07HH323 pKa = 6.66TFSGSEE329 pKa = 4.16INWSLGVMISQFLGVMLMQLLGVMISQFLGVMITQCLGVMISQFLL374 pKa = 3.45

Molecular weight: 37.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A401RMS1|A0A401RMS1_CHIPU Uncharacterized protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0021063 PE=4 SV=1
MM1 pKa = 7.78ASLLLAPNTGRR12 pKa = 11.84MASLLLAPNTGRR24 pKa = 11.84MASLLLAPNAEE35 pKa = 3.99RR36 pKa = 11.84MALLLLAPNAGRR48 pKa = 11.84MALLLLAPNAGRR60 pKa = 11.84MASLLPAPNARR71 pKa = 11.84RR72 pKa = 11.84MDD74 pKa = 4.06SLLLAPNARR83 pKa = 11.84RR84 pKa = 11.84MASLLLALNARR95 pKa = 11.84KK96 pKa = 8.26MVSLLLAPHH105 pKa = 6.96ARR107 pKa = 11.84RR108 pKa = 11.84MASLLLVTQARR119 pKa = 11.84RR120 pKa = 11.84MASLLVAPNARR131 pKa = 11.84RR132 pKa = 11.84MVSLLLAPHH141 pKa = 6.96ARR143 pKa = 11.84RR144 pKa = 11.84MASLLLAPNARR155 pKa = 11.84RR156 pKa = 11.84MASLLLAPHH165 pKa = 6.64FRR167 pKa = 11.84RR168 pKa = 11.84MATLLLAPNARR179 pKa = 11.84RR180 pKa = 11.84MASLLLAPHH189 pKa = 7.03ARR191 pKa = 11.84RR192 pKa = 11.84MASLLLASQVRR203 pKa = 11.84RR204 pKa = 11.84MASLLLAPNARR215 pKa = 11.84RR216 pKa = 11.84MVLMLLSPHH225 pKa = 6.29ARR227 pKa = 11.84RR228 pKa = 11.84MALLLLAPNAGRR240 pKa = 11.84MASLLLAPNAKK251 pKa = 9.81RR252 pKa = 11.84MASLLPALNARR263 pKa = 11.84RR264 pKa = 11.84MALLLLAPNAGRR276 pKa = 11.84MAA278 pKa = 4.48

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33497

0

33497

12024568

30

8400

359.0

40.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.572 ± 0.017

2.23 ± 0.013

5.295 ± 0.022

7.19 ± 0.025

3.425 ± 0.011

6.543 ± 0.025

2.517 ± 0.008

4.873 ± 0.013

5.751 ± 0.018

9.184 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.309 ± 0.007

4.122 ± 0.012

5.247 ± 0.02

4.693 ± 0.017

5.961 ± 0.021

8.21 ± 0.025

5.591 ± 0.018

6.422 ± 0.017

1.203 ± 0.007

2.663 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski