Amino acid dipepetide frequency for Leuconostoc phage LN04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.76AlaAla: 0.76 ± 0.372
0.0AlaCys: 0.0 ± 0.0
4.688AlaAsp: 4.688 ± 0.813
1.774AlaGlu: 1.774 ± 0.46
2.914AlaPhe: 2.914 ± 0.854
4.942AlaGly: 4.942 ± 0.717
0.127AlaHis: 0.127 ± 0.129
5.955AlaIle: 5.955 ± 1.138
3.801AlaLys: 3.801 ± 0.512
5.068AlaLeu: 5.068 ± 0.88
1.647AlaMet: 1.647 ± 0.404
4.942AlaAsn: 4.942 ± 0.721
1.647AlaPro: 1.647 ± 0.378
3.548AlaGln: 3.548 ± 0.71
2.027AlaArg: 2.027 ± 0.445
4.942AlaSer: 4.942 ± 0.873
4.435AlaThr: 4.435 ± 0.975
5.322AlaVal: 5.322 ± 0.895
0.76AlaTrp: 0.76 ± 0.224
3.294AlaTyr: 3.294 ± 0.701
0.0AlaXaa: 0.0 ± 0.0
Cys
0.253CysAla: 0.253 ± 0.205
0.0CysCys: 0.0 ± 0.0
0.127CysAsp: 0.127 ± 0.118
0.0CysGlu: 0.0 ± 0.0
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.253CysHis: 0.253 ± 0.306
0.127CysIle: 0.127 ± 0.142
0.0CysLys: 0.0 ± 0.0
0.127CysLeu: 0.127 ± 0.128
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.127CysGln: 0.127 ± 0.153
0.0CysArg: 0.0 ± 0.0
0.127CysSer: 0.127 ± 0.128
0.127CysThr: 0.127 ± 0.136
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
1.267AspAla: 1.267 ± 0.346
0.253AspCys: 0.253 ± 0.219
4.181AspAsp: 4.181 ± 1.001
4.688AspGlu: 4.688 ± 0.889
4.435AspPhe: 4.435 ± 0.781
4.942AspGly: 4.942 ± 0.893
1.014AspHis: 1.014 ± 0.282
4.942AspIle: 4.942 ± 0.622
5.449AspLys: 5.449 ± 1.05
5.322AspLeu: 5.322 ± 0.994
2.027AspMet: 2.027 ± 0.474
4.942AspAsn: 4.942 ± 0.864
2.281AspPro: 2.281 ± 0.552
0.76AspGln: 0.76 ± 0.369
1.521AspArg: 1.521 ± 0.526
4.181AspSer: 4.181 ± 0.822
3.675AspThr: 3.675 ± 0.737
3.548AspVal: 3.548 ± 0.551
0.887AspTrp: 0.887 ± 0.335
2.914AspTyr: 2.914 ± 0.58
0.0AspXaa: 0.0 ± 0.0
Glu
2.154GluAla: 2.154 ± 0.538
0.127GluCys: 0.127 ± 0.153
2.661GluAsp: 2.661 ± 0.668
2.281GluGlu: 2.281 ± 0.632
2.534GluPhe: 2.534 ± 0.734
1.521GluGly: 1.521 ± 0.455
1.14GluHis: 1.14 ± 0.39
4.308GluIle: 4.308 ± 0.839
3.548GluLys: 3.548 ± 0.764
6.462GluLeu: 6.462 ± 0.95
1.014GluMet: 1.014 ± 0.34
4.562GluAsn: 4.562 ± 0.883
1.394GluPro: 1.394 ± 0.481
2.154GluGln: 2.154 ± 0.468
1.901GluArg: 1.901 ± 0.607
2.914GluSer: 2.914 ± 0.568
3.041GluThr: 3.041 ± 0.702
2.534GluVal: 2.534 ± 0.703
0.76GluTrp: 0.76 ± 0.304
2.281GluTyr: 2.281 ± 0.524
0.0GluXaa: 0.0 ± 0.0
Phe
2.281PheAla: 2.281 ± 0.654
0.253PheCys: 0.253 ± 0.193
3.675PheAsp: 3.675 ± 0.55
2.914PheGlu: 2.914 ± 0.667
1.14PhePhe: 1.14 ± 0.43
4.181PheGly: 4.181 ± 0.652
0.38PheHis: 0.38 ± 0.219
4.181PheIle: 4.181 ± 0.623
4.435PheLys: 4.435 ± 0.695
3.675PheLeu: 3.675 ± 0.635
1.394PheMet: 1.394 ± 0.489
2.661PheAsn: 2.661 ± 0.588
1.14PhePro: 1.14 ± 0.405
1.267PheGln: 1.267 ± 0.441
1.14PheArg: 1.14 ± 0.357
2.914PheSer: 2.914 ± 0.71
3.421PheThr: 3.421 ± 0.606
2.534PheVal: 2.534 ± 0.557
0.507PheTrp: 0.507 ± 0.295
2.027PheTyr: 2.027 ± 0.508
0.0PheXaa: 0.0 ± 0.0
Gly
5.068GlyAla: 5.068 ± 1.497
0.0GlyCys: 0.0 ± 0.0
3.928GlyAsp: 3.928 ± 0.727
1.901GlyGlu: 1.901 ± 0.528
4.308GlyPhe: 4.308 ± 0.974
3.548GlyGly: 3.548 ± 0.971
0.634GlyHis: 0.634 ± 0.252
5.195GlyIle: 5.195 ± 1.413
5.068GlyLys: 5.068 ± 0.854
5.449GlyLeu: 5.449 ± 0.887
1.521GlyMet: 1.521 ± 0.416
3.294GlyAsn: 3.294 ± 0.673
0.127GlyPro: 0.127 ± 0.115
2.914GlyGln: 2.914 ± 0.519
2.661GlyArg: 2.661 ± 0.523
6.589GlySer: 6.589 ± 1.214
5.195GlyThr: 5.195 ± 0.858
5.702GlyVal: 5.702 ± 1.458
0.38GlyTrp: 0.38 ± 0.217
3.041GlyTyr: 3.041 ± 0.877
0.0GlyXaa: 0.0 ± 0.0
His
0.887HisAla: 0.887 ± 0.306
0.127HisCys: 0.127 ± 0.128
1.014HisAsp: 1.014 ± 0.344
0.634HisGlu: 0.634 ± 0.41
0.253HisPhe: 0.253 ± 0.167
1.774HisGly: 1.774 ± 0.569
0.253HisHis: 0.253 ± 0.225
1.394HisIle: 1.394 ± 0.416
0.76HisLys: 0.76 ± 0.374
1.014HisLeu: 1.014 ± 0.4
0.507HisMet: 0.507 ± 0.233
1.014HisAsn: 1.014 ± 0.403
0.127HisPro: 0.127 ± 0.131
0.38HisGln: 0.38 ± 0.229
0.634HisArg: 0.634 ± 0.28
1.267HisSer: 1.267 ± 0.302
1.014HisThr: 1.014 ± 0.403
0.76HisVal: 0.76 ± 0.29
0.0HisTrp: 0.0 ± 0.0
1.14HisTyr: 1.14 ± 0.393
0.0HisXaa: 0.0 ± 0.0
Ile
4.815IleAla: 4.815 ± 0.898
0.0IleCys: 0.0 ± 0.0
4.688IleAsp: 4.688 ± 0.702
3.801IleGlu: 3.801 ± 0.824
3.041IlePhe: 3.041 ± 0.506
5.068IleGly: 5.068 ± 1.539
1.14IleHis: 1.14 ± 0.449
5.449IleIle: 5.449 ± 0.918
6.082IleLys: 6.082 ± 0.891
4.181IleLeu: 4.181 ± 0.627
1.647IleMet: 1.647 ± 0.585
3.928IleAsn: 3.928 ± 0.733
2.154IlePro: 2.154 ± 0.444
3.168IleGln: 3.168 ± 0.485
2.408IleArg: 2.408 ± 0.422
5.322IleSer: 5.322 ± 0.746
5.068IleThr: 5.068 ± 0.859
4.435IleVal: 4.435 ± 0.738
0.634IleTrp: 0.634 ± 0.296
2.914IleTyr: 2.914 ± 0.621
0.0IleXaa: 0.0 ± 0.0
Lys
4.942LysAla: 4.942 ± 0.762
0.127LysCys: 0.127 ± 0.136
3.675LysAsp: 3.675 ± 0.92
2.661LysGlu: 2.661 ± 0.531
3.041LysPhe: 3.041 ± 0.699
4.435LysGly: 4.435 ± 0.645
1.14LysHis: 1.14 ± 0.406
4.435LysIle: 4.435 ± 0.693
4.815LysLys: 4.815 ± 1.137
7.476LysLeu: 7.476 ± 0.87
2.154LysMet: 2.154 ± 0.537
5.068LysAsn: 5.068 ± 0.751
2.914LysPro: 2.914 ± 0.655
3.801LysGln: 3.801 ± 0.753
3.294LysArg: 3.294 ± 0.686
5.449LysSer: 5.449 ± 0.811
5.068LysThr: 5.068 ± 0.667
2.788LysVal: 2.788 ± 0.647
0.634LysTrp: 0.634 ± 0.332
3.168LysTyr: 3.168 ± 0.768
0.0LysXaa: 0.0 ± 0.0
Leu
7.603LeuAla: 7.603 ± 0.902
0.0LeuCys: 0.0 ± 0.0
5.829LeuAsp: 5.829 ± 0.904
4.815LeuGlu: 4.815 ± 0.779
3.294LeuPhe: 3.294 ± 0.65
6.716LeuGly: 6.716 ± 1.194
1.647LeuHis: 1.647 ± 0.42
3.548LeuIle: 3.548 ± 0.772
6.336LeuLys: 6.336 ± 0.801
5.829LeuLeu: 5.829 ± 0.843
2.154LeuMet: 2.154 ± 0.428
4.942LeuAsn: 4.942 ± 0.738
2.534LeuPro: 2.534 ± 0.565
3.548LeuGln: 3.548 ± 0.65
1.901LeuArg: 1.901 ± 0.51
6.209LeuSer: 6.209 ± 0.686
7.096LeuThr: 7.096 ± 1.358
5.575LeuVal: 5.575 ± 1.041
0.634LeuTrp: 0.634 ± 0.276
2.914LeuTyr: 2.914 ± 0.655
0.0LeuXaa: 0.0 ± 0.0
Met
2.788MetAla: 2.788 ± 0.459
0.0MetCys: 0.0 ± 0.0
1.14MetAsp: 1.14 ± 0.46
0.76MetGlu: 0.76 ± 0.256
0.76MetPhe: 0.76 ± 0.379
2.027MetGly: 2.027 ± 0.672
0.253MetHis: 0.253 ± 0.173
1.014MetIle: 1.014 ± 0.399
1.521MetLys: 1.521 ± 0.42
1.014MetLeu: 1.014 ± 0.345
0.38MetMet: 0.38 ± 0.21
1.901MetAsn: 1.901 ± 0.524
1.014MetPro: 1.014 ± 0.368
0.507MetGln: 0.507 ± 0.226
0.76MetArg: 0.76 ± 0.342
2.027MetSer: 2.027 ± 0.543
2.154MetThr: 2.154 ± 0.346
2.154MetVal: 2.154 ± 0.453
0.0MetTrp: 0.0 ± 0.0
1.267MetTyr: 1.267 ± 0.403
0.0MetXaa: 0.0 ± 0.0
Asn
5.449AsnAla: 5.449 ± 0.719
0.0AsnCys: 0.0 ± 0.0
3.421AsnAsp: 3.421 ± 0.654
3.168AsnGlu: 3.168 ± 0.812
2.281AsnPhe: 2.281 ± 0.502
5.829AsnGly: 5.829 ± 0.984
1.14AsnHis: 1.14 ± 0.453
4.562AsnIle: 4.562 ± 0.692
4.562AsnLys: 4.562 ± 0.842
5.322AsnLeu: 5.322 ± 0.568
1.014AsnMet: 1.014 ± 0.385
5.195AsnAsn: 5.195 ± 0.797
2.788AsnPro: 2.788 ± 0.507
3.548AsnGln: 3.548 ± 0.665
1.901AsnArg: 1.901 ± 0.482
3.801AsnSer: 3.801 ± 0.742
4.055AsnThr: 4.055 ± 0.743
5.322AsnVal: 5.322 ± 0.711
0.887AsnTrp: 0.887 ± 0.395
3.675AsnTyr: 3.675 ± 0.696
0.0AsnXaa: 0.0 ± 0.0
Pro
1.647ProAla: 1.647 ± 0.381
0.0ProCys: 0.0 ± 0.0
2.408ProAsp: 2.408 ± 0.589
1.267ProGlu: 1.267 ± 0.582
1.521ProPhe: 1.521 ± 0.45
0.253ProGly: 0.253 ± 0.178
0.634ProHis: 0.634 ± 0.322
2.914ProIle: 2.914 ± 0.457
2.408ProLys: 2.408 ± 0.567
2.788ProLeu: 2.788 ± 0.379
0.634ProMet: 0.634 ± 0.284
1.774ProAsn: 1.774 ± 0.421
0.127ProPro: 0.127 ± 0.128
1.774ProGln: 1.774 ± 0.486
1.014ProArg: 1.014 ± 0.436
3.421ProSer: 3.421 ± 1.004
2.534ProThr: 2.534 ± 0.524
1.647ProVal: 1.647 ± 0.401
0.0ProTrp: 0.0 ± 0.0
1.267ProTyr: 1.267 ± 0.425
0.0ProXaa: 0.0 ± 0.0
Gln
3.801GlnAla: 3.801 ± 0.887
0.127GlnCys: 0.127 ± 0.127
2.661GlnAsp: 2.661 ± 0.53
2.027GlnGlu: 2.027 ± 0.693
1.774GlnPhe: 1.774 ± 0.486
2.027GlnGly: 2.027 ± 0.459
0.0GlnHis: 0.0 ± 0.0
2.534GlnIle: 2.534 ± 0.506
2.914GlnLys: 2.914 ± 0.633
3.928GlnLeu: 3.928 ± 0.841
1.774GlnMet: 1.774 ± 0.413
2.154GlnAsn: 2.154 ± 0.465
1.647GlnPro: 1.647 ± 0.505
2.027GlnGln: 2.027 ± 0.485
2.281GlnArg: 2.281 ± 0.531
3.294GlnSer: 3.294 ± 0.73
3.548GlnThr: 3.548 ± 0.526
2.788GlnVal: 2.788 ± 0.61
0.76GlnTrp: 0.76 ± 0.273
2.027GlnTyr: 2.027 ± 0.599
0.0GlnXaa: 0.0 ± 0.0
Arg
2.154ArgAla: 2.154 ± 0.504
0.0ArgCys: 0.0 ± 0.0
1.901ArgAsp: 1.901 ± 0.581
2.154ArgGlu: 2.154 ± 0.514
1.394ArgPhe: 1.394 ± 0.378
1.901ArgGly: 1.901 ± 0.469
0.507ArgHis: 0.507 ± 0.276
2.281ArgIle: 2.281 ± 0.5
1.774ArgLys: 1.774 ± 0.488
3.928ArgLeu: 3.928 ± 0.755
0.634ArgMet: 0.634 ± 0.257
1.647ArgAsn: 1.647 ± 0.445
1.521ArgPro: 1.521 ± 0.435
2.281ArgGln: 2.281 ± 0.588
0.76ArgArg: 0.76 ± 0.347
1.394ArgSer: 1.394 ± 0.36
2.027ArgThr: 2.027 ± 0.543
2.914ArgVal: 2.914 ± 0.541
0.76ArgTrp: 0.76 ± 0.351
1.394ArgTyr: 1.394 ± 0.512
0.0ArgXaa: 0.0 ± 0.0
Ser
4.942SerAla: 4.942 ± 0.64
0.0SerCys: 0.0 ± 0.0
5.322SerAsp: 5.322 ± 0.836
4.688SerGlu: 4.688 ± 1.177
2.408SerPhe: 2.408 ± 0.645
5.575SerGly: 5.575 ± 1.619
1.647SerHis: 1.647 ± 0.411
5.449SerIle: 5.449 ± 0.847
5.068SerLys: 5.068 ± 0.761
6.082SerLeu: 6.082 ± 1.247
1.774SerMet: 1.774 ± 0.509
4.815SerAsn: 4.815 ± 1.007
1.774SerPro: 1.774 ± 0.571
4.308SerGln: 4.308 ± 0.77
2.027SerArg: 2.027 ± 0.476
5.322SerSer: 5.322 ± 1.111
5.955SerThr: 5.955 ± 1.02
7.096SerVal: 7.096 ± 1.249
0.38SerTrp: 0.38 ± 0.216
2.661SerTyr: 2.661 ± 0.51
0.0SerXaa: 0.0 ± 0.0
Thr
4.308ThrAla: 4.308 ± 0.695
0.127ThrCys: 0.127 ± 0.153
3.801ThrAsp: 3.801 ± 0.598
3.168ThrGlu: 3.168 ± 0.565
3.928ThrPhe: 3.928 ± 0.609
5.195ThrGly: 5.195 ± 0.665
1.14ThrHis: 1.14 ± 0.471
4.815ThrIle: 4.815 ± 0.935
4.688ThrLys: 4.688 ± 0.97
5.449ThrLeu: 5.449 ± 0.748
1.14ThrMet: 1.14 ± 0.37
5.829ThrAsn: 5.829 ± 0.688
2.281ThrPro: 2.281 ± 0.346
3.421ThrGln: 3.421 ± 0.615
3.294ThrArg: 3.294 ± 0.721
7.349ThrSer: 7.349 ± 1.671
5.322ThrThr: 5.322 ± 0.803
4.688ThrVal: 4.688 ± 0.766
0.634ThrTrp: 0.634 ± 0.279
2.534ThrTyr: 2.534 ± 0.557
0.0ThrXaa: 0.0 ± 0.0
Val
4.055ValAla: 4.055 ± 0.652
0.0ValCys: 0.0 ± 0.0
4.815ValAsp: 4.815 ± 0.8
3.041ValGlu: 3.041 ± 0.779
4.055ValPhe: 4.055 ± 0.74
3.294ValGly: 3.294 ± 1.048
0.76ValHis: 0.76 ± 0.318
3.548ValIle: 3.548 ± 0.778
5.322ValLys: 5.322 ± 0.774
4.435ValLeu: 4.435 ± 0.626
1.267ValMet: 1.267 ± 0.405
5.322ValAsn: 5.322 ± 1.157
3.041ValPro: 3.041 ± 0.515
2.661ValGln: 2.661 ± 0.572
1.774ValArg: 1.774 ± 0.589
5.829ValSer: 5.829 ± 0.805
5.829ValThr: 5.829 ± 1.044
4.942ValVal: 4.942 ± 0.713
0.507ValTrp: 0.507 ± 0.218
3.548ValTyr: 3.548 ± 0.699
0.0ValXaa: 0.0 ± 0.0
Trp
0.507TrpAla: 0.507 ± 0.228
0.127TrpCys: 0.127 ± 0.136
0.634TrpAsp: 0.634 ± 0.256
0.76TrpGlu: 0.76 ± 0.339
0.38TrpPhe: 0.38 ± 0.213
0.76TrpGly: 0.76 ± 0.31
0.38TrpHis: 0.38 ± 0.209
0.507TrpIle: 0.507 ± 0.242
0.0TrpLys: 0.0 ± 0.0
1.14TrpLeu: 1.14 ± 0.437
0.127TrpMet: 0.127 ± 0.127
0.887TrpAsn: 0.887 ± 0.338
0.0TrpPro: 0.0 ± 0.0
0.507TrpGln: 0.507 ± 0.322
0.507TrpArg: 0.507 ± 0.371
1.267TrpSer: 1.267 ± 0.388
0.38TrpThr: 0.38 ± 0.219
0.507TrpVal: 0.507 ± 0.301
0.253TrpTrp: 0.253 ± 0.167
0.38TrpTyr: 0.38 ± 0.231
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.914TyrAla: 2.914 ± 0.6
0.0TyrCys: 0.0 ± 0.0
2.661TyrAsp: 2.661 ± 0.74
2.788TyrGlu: 2.788 ± 0.802
2.661TyrPhe: 2.661 ± 0.586
2.534TyrGly: 2.534 ± 0.764
0.76TyrHis: 0.76 ± 0.386
2.788TyrIle: 2.788 ± 0.529
2.661TyrLys: 2.661 ± 0.634
4.435TyrLeu: 4.435 ± 0.687
0.507TyrMet: 0.507 ± 0.239
3.041TyrAsn: 3.041 ± 0.756
1.521TyrPro: 1.521 ± 0.414
1.394TyrGln: 1.394 ± 0.5
1.521TyrArg: 1.521 ± 0.542
3.675TyrSer: 3.675 ± 0.8
3.168TyrThr: 3.168 ± 0.766
2.788TyrVal: 2.788 ± 0.595
0.634TyrTrp: 0.634 ± 0.279
2.154TyrTyr: 2.154 ± 0.707
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 39 proteins (7893 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski