Embleya scabrispora

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Embleya

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9009 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T3P4U8|A0A1T3P4U8_9ACTN Cobalamin-binding protein OS=Embleya scabrispora OX=159449 GN=B4N89_27155 PE=4 SV=1
MM1 pKa = 6.95TAWLPTAHH9 pKa = 6.82ATPPTNVPTTAADD22 pKa = 3.57YY23 pKa = 9.4GARR26 pKa = 11.84WLASRR31 pKa = 11.84FVSDD35 pKa = 3.49GNGNKK40 pKa = 10.15FIPATGSPTTPEE52 pKa = 4.41PSNTMQAALALAAAGVEE69 pKa = 4.09EE70 pKa = 4.53ATFDD74 pKa = 5.53DD75 pKa = 4.62AVDD78 pKa = 3.38WLKK81 pKa = 11.69ANVTTTIAPGGTDD94 pKa = 2.91NPGRR98 pKa = 11.84LGYY101 pKa = 10.72LLLVADD107 pKa = 4.99AAGEE111 pKa = 4.39SPTSFGGVDD120 pKa = 3.5LVTRR124 pKa = 11.84LGNLRR129 pKa = 11.84QTTGADD135 pKa = 2.77TGLFGSADD143 pKa = 3.52PTYY146 pKa = 11.04DD147 pKa = 3.0GTFRR151 pKa = 11.84QSLAILGLRR160 pKa = 11.84AVGQSVDD167 pKa = 3.52SSSTTWLLGQQCAGGSNADD186 pKa = 3.53DD187 pKa = 4.44GGWRR191 pKa = 11.84SYY193 pKa = 11.33GGSTCDD199 pKa = 5.35GDD201 pKa = 5.2DD202 pKa = 3.72ADD204 pKa = 4.54SNSTAFAIQALVSVSHH220 pKa = 5.29TTYY223 pKa = 10.55SGEE226 pKa = 4.18VGDD229 pKa = 4.49ALGWLDD235 pKa = 5.0AVQEE239 pKa = 4.19SDD241 pKa = 4.89AGFAYY246 pKa = 10.1QPAYY250 pKa = 10.51ASDD253 pKa = 4.17PNSTSLVIQAIIAGGGNPTSATWQKK278 pKa = 11.32SGGTLNPYY286 pKa = 9.65TSLLSWQIGCTANPGDD302 pKa = 3.78VGAFASPWSSGAPDD316 pKa = 4.64LLATQQSVWGAAGAPFPLNPSFTTPTPTPCIPP348 pKa = 3.59

Molecular weight: 35.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T3NJ67|A0A1T3NJ67_9ACTN Uncharacterized protein OS=Embleya scabrispora OX=159449 GN=B4N89_45485 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84ARR4 pKa = 11.84RR5 pKa = 11.84WSIMPRR11 pKa = 11.84TAVPSRR17 pKa = 11.84WRR19 pKa = 11.84TLMPGTTNPARR30 pKa = 11.84VLGRR34 pKa = 11.84SASSVGTGGAVKK46 pKa = 10.59LMVGVPLVFSTTRR59 pKa = 11.84TGG61 pKa = 3.23

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9009

0

9009

2957195

29

3488

328.2

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.975 ± 0.043

0.823 ± 0.007

6.256 ± 0.02

5.35 ± 0.026

2.69 ± 0.014

9.502 ± 0.029

2.383 ± 0.016

3.362 ± 0.015

1.723 ± 0.021

10.066 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.724 ± 0.011

1.718 ± 0.014

6.514 ± 0.031

2.225 ± 0.014

8.697 ± 0.028

4.687 ± 0.019

6.235 ± 0.026

8.615 ± 0.026

1.516 ± 0.011

1.939 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski