Anabas testudineus (Climbing perch) (Anthias testudineus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61942 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q1I070|A0A3Q1I070_ANATE RNA-binding protein 39-like OS=Anabas testudineus OX=64144 PE=4 SV=1
MM1 pKa = 7.39YY2 pKa = 10.41LEE4 pKa = 5.13NSEE7 pKa = 4.01QTDD10 pKa = 3.08ISGEE14 pKa = 4.17RR15 pKa = 11.84EE16 pKa = 3.92LSTEE20 pKa = 3.71AMLAEE25 pKa = 4.24QCQVPKK31 pKa = 9.79LTEE34 pKa = 3.96AADD37 pKa = 3.98LNDD40 pKa = 3.58ASKK43 pKa = 11.28GSAEE47 pKa = 3.86LGIRR51 pKa = 11.84VLVEE55 pKa = 3.46YY56 pKa = 10.31TEE58 pKa = 4.14QLKK61 pKa = 10.03SRR63 pKa = 11.84KK64 pKa = 8.5MDD66 pKa = 4.13FSLLCDD72 pKa = 3.84EE73 pKa = 4.62QCNMTRR79 pKa = 11.84DD80 pKa = 3.71EE81 pKa = 5.05IEE83 pKa = 3.92TFTYY87 pKa = 10.72CNGHH91 pKa = 6.42AEE93 pKa = 4.26SFEE96 pKa = 4.21QYY98 pKa = 9.95SASNGFFEE106 pKa = 4.84SDD108 pKa = 3.08QTIDD112 pKa = 2.92HH113 pKa = 6.98CEE115 pKa = 3.91TLRR118 pKa = 11.84LSQQFDD124 pKa = 3.32EE125 pKa = 4.92CAQHH129 pKa = 6.66CEE131 pKa = 4.13CFKK134 pKa = 9.87PCKK137 pKa = 10.07SSEE140 pKa = 3.83HH141 pKa = 6.43CANHH145 pKa = 6.54SLTSTCNSCCEE156 pKa = 4.01HH157 pKa = 6.74CVEE160 pKa = 4.82HH161 pKa = 6.72LQLFQQCKK169 pKa = 9.83PPDD172 pKa = 3.48QQFEE176 pKa = 4.47SFDD179 pKa = 3.68IEE181 pKa = 3.8PDD183 pKa = 3.5KK184 pKa = 11.27PVMNGDD190 pKa = 3.53NLEE193 pKa = 4.04QCEE196 pKa = 4.3MSDD199 pKa = 5.63LIPEE203 pKa = 4.43STDD206 pKa = 2.95HH207 pKa = 7.48FEE209 pKa = 4.56VLQQYY214 pKa = 9.62EE215 pKa = 4.41PTDD218 pKa = 3.76EE219 pKa = 3.97QCEE222 pKa = 4.37CFDD225 pKa = 4.92CEE227 pKa = 4.35PDD229 pKa = 3.09TSTEE233 pKa = 3.93HH234 pKa = 6.65SEE236 pKa = 3.95HH237 pKa = 8.23SEE239 pKa = 3.97MTGFTPDD246 pKa = 3.08TSDD249 pKa = 4.41SVDD252 pKa = 3.5LLDD255 pKa = 5.14YY256 pKa = 9.4GTEE259 pKa = 3.76FCEE262 pKa = 4.73YY263 pKa = 11.03DD264 pKa = 4.05DD265 pKa = 4.35IQNEE269 pKa = 4.38VATNANDD276 pKa = 3.62YY277 pKa = 11.19DD278 pKa = 4.71YY279 pKa = 11.69DD280 pKa = 6.21DD281 pKa = 6.4DD282 pKa = 7.17DD283 pKa = 6.78DD284 pKa = 6.33DD285 pKa = 5.3YY286 pKa = 12.12SEE288 pKa = 4.49NCPLEE293 pKa = 4.06QNEE296 pKa = 4.56TEE298 pKa = 4.17ASDD301 pKa = 5.36DD302 pKa = 3.68EE303 pKa = 4.6SCRR306 pKa = 11.84LSEE309 pKa = 4.55DD310 pKa = 3.73VSSSSCNTPVHH321 pKa = 6.8IYY323 pKa = 10.78YY324 pKa = 10.5DD325 pKa = 4.13DD326 pKa = 4.29NEE328 pKa = 4.57HH329 pKa = 6.19VCLFDD334 pKa = 4.7EE335 pKa = 4.53RR336 pKa = 11.84CEE338 pKa = 5.62DD339 pKa = 3.59YY340 pKa = 11.25LQTNEE345 pKa = 3.95QQAATLDD352 pKa = 3.76VSTDD356 pKa = 3.0HH357 pKa = 7.61CEE359 pKa = 3.91TCGNDD364 pKa = 3.38DD365 pKa = 3.66TEE367 pKa = 4.69TNQEE371 pKa = 4.28CEE373 pKa = 4.2PTQQCSDD380 pKa = 3.29KK381 pKa = 10.03TSEE384 pKa = 3.97FGTEE388 pKa = 4.18EE389 pKa = 4.39EE390 pKa = 4.81CSSDD394 pKa = 3.64CSSMEE399 pKa = 4.05TKK401 pKa = 10.68SFTTCPDD408 pKa = 2.76GSIPSDD414 pKa = 3.49HH415 pKa = 6.91YY416 pKa = 11.24SDD418 pKa = 4.62SSRR421 pKa = 11.84EE422 pKa = 3.86SDD424 pKa = 3.71KK425 pKa = 11.35GVQEE429 pKa = 5.27DD430 pKa = 4.03SSDD433 pKa = 4.0DD434 pKa = 3.54QTEE437 pKa = 4.24WEE439 pKa = 4.41SFEE442 pKa = 5.04EE443 pKa = 4.9DD444 pKa = 3.44EE445 pKa = 4.23QTEE448 pKa = 4.14QIKK451 pKa = 10.69INEE454 pKa = 4.32SNEE457 pKa = 4.07EE458 pKa = 4.02EE459 pKa = 4.52KK460 pKa = 9.84KK461 pKa = 9.33TPTGDD466 pKa = 3.43IVIEE470 pKa = 4.49DD471 pKa = 3.77YY472 pKa = 11.38FDD474 pKa = 4.13FFDD477 pKa = 3.52RR478 pKa = 11.84TDD480 pKa = 3.34YY481 pKa = 11.42YY482 pKa = 11.07RR483 pKa = 11.84DD484 pKa = 3.27KK485 pKa = 10.91FSQKK489 pKa = 8.27RR490 pKa = 11.84HH491 pKa = 5.95YY492 pKa = 10.19ISCFDD497 pKa = 3.9GGDD500 pKa = 3.15IHH502 pKa = 7.66DD503 pKa = 4.72CLHH506 pKa = 7.72LEE508 pKa = 4.43EE509 pKa = 5.66KK510 pKa = 9.82PQKK513 pKa = 10.4CSAKK517 pKa = 9.62NAHH520 pKa = 6.18GFEE523 pKa = 5.11KK524 pKa = 9.77ITVHH528 pKa = 6.09EE529 pKa = 4.81ADD531 pKa = 3.74VCSDD535 pKa = 3.41APEE538 pKa = 4.16EE539 pKa = 4.13ASQITSEE546 pKa = 4.14EE547 pKa = 4.24DD548 pKa = 2.81EE549 pKa = 4.45SLRR552 pKa = 11.84GDD554 pKa = 3.88SEE556 pKa = 4.64TEE558 pKa = 3.9KK559 pKa = 11.14NPEE562 pKa = 3.7DD563 pKa = 3.34WSIDD567 pKa = 3.93SEE569 pKa = 4.96SGLADD574 pKa = 4.06DD575 pKa = 6.06DD576 pKa = 3.86IEE578 pKa = 4.54EE579 pKa = 4.4IEE581 pKa = 4.74RR582 pKa = 11.84EE583 pKa = 4.28SCADD587 pKa = 3.52DD588 pKa = 3.83YY589 pKa = 12.1EE590 pKa = 4.4EE591 pKa = 6.07AEE593 pKa = 4.18EE594 pKa = 4.6DD595 pKa = 3.68ADD597 pKa = 4.82GEE599 pKa = 4.62TFDD602 pKa = 5.87EE603 pKa = 4.72EE604 pKa = 4.94ACVFDD609 pKa = 5.28GDD611 pKa = 3.89VCEE614 pKa = 4.66ICHH617 pKa = 6.14EE618 pKa = 4.52DD619 pKa = 3.37EE620 pKa = 5.73AEE622 pKa = 4.22VFSSSGNEE630 pKa = 3.73EE631 pKa = 4.47SMCAPCADD639 pKa = 4.33NISVEE644 pKa = 3.75GDD646 pKa = 3.07AYY648 pKa = 10.51EE649 pKa = 4.95DD650 pKa = 3.35EE651 pKa = 6.07GYY653 pKa = 10.63DD654 pKa = 3.87ALDD657 pKa = 3.88NEE659 pKa = 4.77SLGDD663 pKa = 3.77NTSSTDD669 pKa = 3.46QLQTTVIDD677 pKa = 3.93CKK679 pKa = 10.87KK680 pKa = 10.37DD681 pKa = 3.62NEE683 pKa = 4.52PGAEE687 pKa = 3.77DD688 pKa = 3.59TTFIEE693 pKa = 4.69CSEE696 pKa = 4.14IEE698 pKa = 4.6AYY700 pKa = 9.04WLLKK704 pKa = 10.69DD705 pKa = 3.42NEE707 pKa = 4.32YY708 pKa = 11.23NIGEE712 pKa = 4.19RR713 pKa = 11.84CEE715 pKa = 4.35SDD717 pKa = 3.38VEE719 pKa = 4.19EE720 pKa = 5.19YY721 pKa = 10.29YY722 pKa = 11.07ACQIRR727 pKa = 11.84SIQSSGKK734 pKa = 8.63QALNEE739 pKa = 3.83FMMQGQSYY747 pKa = 10.22DD748 pKa = 3.68QIIHH752 pKa = 6.58GNTNGDD758 pKa = 3.08ASRR761 pKa = 11.84SEE763 pKa = 4.17RR764 pKa = 11.84EE765 pKa = 3.84GALFPLEE772 pKa = 4.01EE773 pKa = 5.34LEE775 pKa = 4.46TEE777 pKa = 4.79GVCPEE782 pKa = 3.97EE783 pKa = 4.55SKK785 pKa = 9.9TGSFRR790 pKa = 11.84NTEE793 pKa = 3.98VGEE796 pKa = 4.01LAEE799 pKa = 5.05NLASYY804 pKa = 10.99CDD806 pKa = 3.74VEE808 pKa = 4.43NATDD812 pKa = 3.72EE813 pKa = 4.21QTQNSDD819 pKa = 3.83DD820 pKa = 3.79EE821 pKa = 4.59LKK823 pKa = 10.85EE824 pKa = 3.97VLRR827 pKa = 11.84KK828 pKa = 9.3IAPPVGIIHH837 pKa = 6.12SVVSEE842 pKa = 3.89HH843 pKa = 6.63AKK845 pKa = 9.96IAGRR849 pKa = 11.84DD850 pKa = 3.44AKK852 pKa = 10.38TEE854 pKa = 4.12EE855 pKa = 4.49IEE857 pKa = 5.45DD858 pKa = 3.96NEE860 pKa = 4.09QSSDD864 pKa = 3.82SEE866 pKa = 4.5EE867 pKa = 3.98EE868 pKa = 3.9QSDD871 pKa = 3.92NEE873 pKa = 4.22SFEE876 pKa = 4.15PCEE879 pKa = 4.51CEE881 pKa = 3.69HH882 pKa = 6.57CVPLIEE888 pKa = 4.62QVPAKK893 pKa = 10.16PLLPRR898 pKa = 11.84MKK900 pKa = 10.8SNDD903 pKa = 3.18AGKK906 pKa = 9.31ICVVIDD912 pKa = 3.71LDD914 pKa = 3.74EE915 pKa = 4.47TLVHH919 pKa = 7.06SSFKK923 pKa = 10.36PVNNADD929 pKa = 4.05FIIPVEE935 pKa = 3.92IDD937 pKa = 3.4GTVHH941 pKa = 5.33QVYY944 pKa = 9.86VLKK947 pKa = 10.52RR948 pKa = 11.84PHH950 pKa = 7.15VDD952 pKa = 2.95EE953 pKa = 4.17FLKK956 pKa = 10.99RR957 pKa = 11.84MGEE960 pKa = 3.96LFEE963 pKa = 5.41CVLFTASLAKK973 pKa = 10.69YY974 pKa = 9.38ADD976 pKa = 4.0PVSDD980 pKa = 6.19LLDD983 pKa = 3.01KK984 pKa = 10.82WGVFRR989 pKa = 11.84SRR991 pKa = 11.84LFRR994 pKa = 11.84EE995 pKa = 3.9SCVFHH1000 pKa = 6.89KK1001 pKa = 10.9GNYY1004 pKa = 9.11VKK1006 pKa = 10.65DD1007 pKa = 3.95LSRR1010 pKa = 11.84LGRR1013 pKa = 11.84DD1014 pKa = 3.3LNKK1017 pKa = 10.22VIILDD1022 pKa = 3.92NSPASYY1028 pKa = 10.25IFHH1031 pKa = 8.0PDD1033 pKa = 2.66NAVPVASWFDD1043 pKa = 3.67DD1044 pKa = 3.6MSDD1047 pKa = 3.67TEE1049 pKa = 5.5LLDD1052 pKa = 3.75LLPFFEE1058 pKa = 5.11RR1059 pKa = 11.84LSKK1062 pKa = 10.91VDD1064 pKa = 4.9DD1065 pKa = 4.3IYY1067 pKa = 11.43DD1068 pKa = 3.31ILKK1071 pKa = 8.97QQRR1074 pKa = 11.84TSSS1077 pKa = 3.31

Molecular weight: 121.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7N6B1J7|A0A7N6B1J7_ANATE Ribosome production factor 1 homolog OS=Anabas testudineus OX=64144 GN=RPF1 PE=4 SV=1
RR1 pKa = 7.34SKK3 pKa = 9.99STALSSKK10 pKa = 9.01STALSRR16 pKa = 11.84SKK18 pKa = 9.84STALSRR24 pKa = 11.84SKK26 pKa = 10.11GTALSRR32 pKa = 11.84SKK34 pKa = 9.88STALSRR40 pKa = 11.84SKK42 pKa = 9.84STALSRR48 pKa = 11.84SKK50 pKa = 9.84STALSRR56 pKa = 11.84SKK58 pKa = 9.81STASSRR64 pKa = 11.84SKK66 pKa = 9.95GTALSRR72 pKa = 11.84SKK74 pKa = 10.16GTALSRR80 pKa = 11.84SKK82 pKa = 9.83STASSRR88 pKa = 11.84SKK90 pKa = 9.95GTALSRR96 pKa = 11.84SKK98 pKa = 9.88STALSRR104 pKa = 11.84SKK106 pKa = 10.11GTALSRR112 pKa = 11.84SKK114 pKa = 9.88STALSRR120 pKa = 11.84SKK122 pKa = 9.84STALSRR128 pKa = 11.84SKK130 pKa = 10.11GTALSRR136 pKa = 11.84SKK138 pKa = 9.88STALSRR144 pKa = 11.84SKK146 pKa = 9.92STALL150 pKa = 3.54

Molecular weight: 15.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23204

38738

61942

42338991

17

6581

683.5

76.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.32 ± 0.007

2.316 ± 0.008

5.247 ± 0.006

6.754 ± 0.012

3.837 ± 0.007

6.182 ± 0.012

2.641 ± 0.004

4.746 ± 0.006

5.702 ± 0.01

9.743 ± 0.011

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.004

4.016 ± 0.005

5.336 ± 0.01

4.679 ± 0.007

5.455 ± 0.007

8.291 ± 0.01

5.686 ± 0.007

6.508 ± 0.007

1.192 ± 0.004

2.948 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski