Thermoproteus spherical piliferous virus 1

Taxonomy: Viruses; Globuloviridae; Alphaglobulovirus; Alphaglobulovirus TSPV1

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8J451|A0A6G8J451_9VIRU Uncharacterized protein OS=Thermoproteus spherical piliferous virus 1 OX=2713157 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 3.93MEE4 pKa = 6.25GDD6 pKa = 3.46GGGGYY11 pKa = 8.02SVKK14 pKa = 10.39HH15 pKa = 5.81ALFALFLVGLALIYY29 pKa = 10.75VGAALAQVDD38 pKa = 4.12GGLSLMLIFVGIWAAIAAIAIGVFSFMARR67 pKa = 11.84EE68 pKa = 4.1VLGLPP73 pKa = 4.12

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8J394|A0A6G8J394_9VIRU Uncharacterized protein OS=Thermoproteus spherical piliferous virus 1 OX=2713157 PE=4 SV=1
MM1 pKa = 7.74PKK3 pKa = 9.96LAKK6 pKa = 9.84RR7 pKa = 11.84RR8 pKa = 11.84LNMSILCRR16 pKa = 11.84FICAHH21 pKa = 6.63KK22 pKa = 10.78GSVAYY27 pKa = 9.64IYY29 pKa = 8.27PHH31 pKa = 6.46KK32 pKa = 10.98VRR34 pKa = 11.84VFMEE38 pKa = 4.01NDD40 pKa = 3.31TPVVVGGVTAQGLRR54 pKa = 11.84RR55 pKa = 11.84LMRR58 pKa = 11.84RR59 pKa = 11.84LEE61 pKa = 3.94PAIMHH66 pKa = 6.27TFFDD70 pKa = 3.96NKK72 pKa = 9.81RR73 pKa = 11.84GRR75 pKa = 11.84VYY77 pKa = 10.4MVDD80 pKa = 2.46IKK82 pKa = 9.43TAKK85 pKa = 9.53EE86 pKa = 3.43ICRR89 pKa = 11.84KK90 pKa = 8.37YY91 pKa = 10.97VRR93 pKa = 4.23

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

5233

40

533

168.8

18.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.931 ± 0.816

1.261 ± 0.206

3.497 ± 0.486

3.344 ± 0.483

3.44 ± 0.341

7.797 ± 0.373

1.147 ± 0.263

7.071 ± 0.575

2.981 ± 0.422

10.243 ± 1.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.077 ± 0.204

3.172 ± 0.48

5.236 ± 0.358

2.102 ± 0.252

5.007 ± 0.683

5.848 ± 0.478

6.249 ± 0.702

10.396 ± 0.347

1.586 ± 0.186

5.618 ± 0.488
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski