Amino acid dipepetide frequency for Synechococcus phage S-CBP2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.155AlaAla: 12.155 ± 1.225
0.93AlaCys: 0.93 ± 0.292
5.363AlaAsp: 5.363 ± 0.842
6.006AlaGlu: 6.006 ± 0.86
3.504AlaPhe: 3.504 ± 0.588
6.578AlaGly: 6.578 ± 0.785
1.502AlaHis: 1.502 ± 0.376
5.434AlaIle: 5.434 ± 0.47
4.934AlaLys: 4.934 ± 0.687
8.866AlaLeu: 8.866 ± 0.891
2.145AlaMet: 2.145 ± 0.435
4.648AlaAsn: 4.648 ± 0.621
4.791AlaPro: 4.791 ± 0.846
6.936AlaGln: 6.936 ± 0.884
4.576AlaArg: 4.576 ± 0.803
5.792AlaSer: 5.792 ± 0.682
6.292AlaThr: 6.292 ± 0.912
7.651AlaVal: 7.651 ± 0.74
1.287AlaTrp: 1.287 ± 0.29
2.36AlaTyr: 2.36 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
0.715CysAla: 0.715 ± 0.196
0.286CysCys: 0.286 ± 0.14
0.644CysAsp: 0.644 ± 0.241
0.358CysGlu: 0.358 ± 0.171
0.644CysPhe: 0.644 ± 0.281
0.93CysGly: 0.93 ± 0.261
0.143CysHis: 0.143 ± 0.106
0.501CysIle: 0.501 ± 0.184
0.358CysLys: 0.358 ± 0.145
1.073CysLeu: 1.073 ± 0.36
0.143CysMet: 0.143 ± 0.101
0.286CysAsn: 0.286 ± 0.172
0.358CysPro: 0.358 ± 0.157
0.286CysGln: 0.286 ± 0.126
0.572CysArg: 0.572 ± 0.23
0.787CysSer: 0.787 ± 0.239
0.715CysThr: 0.715 ± 0.236
0.787CysVal: 0.787 ± 0.23
0.0CysTrp: 0.0 ± 0.0
0.215CysTyr: 0.215 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
6.435AspAla: 6.435 ± 0.682
0.715AspCys: 0.715 ± 0.303
3.218AspAsp: 3.218 ± 0.452
5.291AspGlu: 5.291 ± 0.648
2.288AspPhe: 2.288 ± 0.572
5.148AspGly: 5.148 ± 0.615
0.858AspHis: 0.858 ± 0.286
2.789AspIle: 2.789 ± 0.473
2.789AspLys: 2.789 ± 0.463
5.22AspLeu: 5.22 ± 0.567
1.216AspMet: 1.216 ± 0.278
2.217AspAsn: 2.217 ± 0.457
3.861AspPro: 3.861 ± 0.671
2.288AspGln: 2.288 ± 0.336
2.789AspArg: 2.789 ± 0.45
3.075AspSer: 3.075 ± 0.455
3.218AspThr: 3.218 ± 0.509
3.718AspVal: 3.718 ± 0.371
1.144AspTrp: 1.144 ± 0.288
2.288AspTyr: 2.288 ± 0.359
0.0AspXaa: 0.0 ± 0.0
Glu
8.509GluAla: 8.509 ± 0.812
0.787GluCys: 0.787 ± 0.227
2.789GluAsp: 2.789 ± 0.608
4.719GluGlu: 4.719 ± 0.742
2.86GluPhe: 2.86 ± 0.4
4.576GluGly: 4.576 ± 0.647
0.787GluHis: 0.787 ± 0.271
3.218GluIle: 3.218 ± 0.646
3.075GluLys: 3.075 ± 0.486
6.435GluLeu: 6.435 ± 0.69
1.931GluMet: 1.931 ± 0.497
1.502GluAsn: 1.502 ± 0.343
1.645GluPro: 1.645 ± 0.313
3.003GluGln: 3.003 ± 0.587
4.433GluArg: 4.433 ± 0.491
2.288GluSer: 2.288 ± 0.332
3.289GluThr: 3.289 ± 0.476
3.718GluVal: 3.718 ± 0.41
1.287GluTrp: 1.287 ± 0.341
1.716GluTyr: 1.716 ± 0.327
0.0GluXaa: 0.0 ± 0.0
Phe
2.789PheAla: 2.789 ± 0.441
0.286PheCys: 0.286 ± 0.147
3.504PheAsp: 3.504 ± 0.514
1.573PheGlu: 1.573 ± 0.252
1.43PhePhe: 1.43 ± 0.348
2.145PheGly: 2.145 ± 0.36
0.644PheHis: 0.644 ± 0.222
1.716PheIle: 1.716 ± 0.331
1.931PheLys: 1.931 ± 0.287
3.218PheLeu: 3.218 ± 0.412
0.429PheMet: 0.429 ± 0.186
1.788PheAsn: 1.788 ± 0.26
1.502PhePro: 1.502 ± 0.338
1.645PheGln: 1.645 ± 0.35
2.86PheArg: 2.86 ± 0.475
1.716PheSer: 1.716 ± 0.331
3.003PheThr: 3.003 ± 0.414
2.002PheVal: 2.002 ± 0.319
0.215PheTrp: 0.215 ± 0.122
1.001PheTyr: 1.001 ± 0.455
0.0PheXaa: 0.0 ± 0.0
Gly
6.507GlyAla: 6.507 ± 0.856
0.787GlyCys: 0.787 ± 0.261
4.791GlyAsp: 4.791 ± 0.493
4.004GlyGlu: 4.004 ± 0.492
2.789GlyPhe: 2.789 ± 0.489
6.65GlyGly: 6.65 ± 0.899
1.216GlyHis: 1.216 ± 0.318
3.361GlyIle: 3.361 ± 0.51
5.077GlyLys: 5.077 ± 0.735
6.578GlyLeu: 6.578 ± 0.631
1.359GlyMet: 1.359 ± 0.272
3.003GlyAsn: 3.003 ± 0.51
2.789GlyPro: 2.789 ± 0.48
3.003GlyGln: 3.003 ± 0.439
4.934GlyArg: 4.934 ± 0.502
5.506GlySer: 5.506 ± 0.921
6.006GlyThr: 6.006 ± 0.897
4.505GlyVal: 4.505 ± 0.571
0.93GlyTrp: 0.93 ± 0.294
2.574GlyTyr: 2.574 ± 0.403
0.0GlyXaa: 0.0 ± 0.0
His
0.93HisAla: 0.93 ± 0.274
0.358HisCys: 0.358 ± 0.163
1.073HisAsp: 1.073 ± 0.276
0.858HisGlu: 0.858 ± 0.306
0.501HisPhe: 0.501 ± 0.199
0.93HisGly: 0.93 ± 0.256
0.286HisHis: 0.286 ± 0.131
0.715HisIle: 0.715 ± 0.203
0.858HisLys: 0.858 ± 0.238
1.716HisLeu: 1.716 ± 0.439
0.286HisMet: 0.286 ± 0.186
0.572HisAsn: 0.572 ± 0.233
0.787HisPro: 0.787 ± 0.256
1.144HisGln: 1.144 ± 0.352
1.001HisArg: 1.001 ± 0.242
0.858HisSer: 0.858 ± 0.243
1.001HisThr: 1.001 ± 0.239
1.073HisVal: 1.073 ± 0.256
0.215HisTrp: 0.215 ± 0.135
0.787HisTyr: 0.787 ± 0.292
0.0HisXaa: 0.0 ± 0.0
Ile
4.362IleAla: 4.362 ± 0.692
0.286IleCys: 0.286 ± 0.154
4.576IleAsp: 4.576 ± 0.499
3.003IleGlu: 3.003 ± 0.511
0.858IlePhe: 0.858 ± 0.264
3.861IleGly: 3.861 ± 0.554
0.787IleHis: 0.787 ± 0.2
1.859IleIle: 1.859 ± 0.393
1.859IleLys: 1.859 ± 0.394
3.432IleLeu: 3.432 ± 0.496
0.93IleMet: 0.93 ± 0.259
1.502IleAsn: 1.502 ± 0.267
2.431IlePro: 2.431 ± 0.432
1.931IleGln: 1.931 ± 0.426
2.36IleArg: 2.36 ± 0.414
3.647IleSer: 3.647 ± 0.414
3.289IleThr: 3.289 ± 0.416
2.431IleVal: 2.431 ± 0.486
0.715IleTrp: 0.715 ± 0.215
2.074IleTyr: 2.074 ± 0.387
0.0IleXaa: 0.0 ± 0.0
Lys
5.506LysAla: 5.506 ± 0.695
0.143LysCys: 0.143 ± 0.089
2.217LysAsp: 2.217 ± 0.496
3.79LysGlu: 3.79 ± 0.47
2.145LysPhe: 2.145 ± 0.41
2.86LysGly: 2.86 ± 0.426
1.073LysHis: 1.073 ± 0.263
1.502LysIle: 1.502 ± 0.301
3.218LysLys: 3.218 ± 0.653
5.935LysLeu: 5.935 ± 0.655
1.216LysMet: 1.216 ± 0.329
2.789LysAsn: 2.789 ± 0.489
3.361LysPro: 3.361 ± 0.405
2.288LysGln: 2.288 ± 0.425
2.002LysArg: 2.002 ± 0.471
2.86LysSer: 2.86 ± 0.391
2.789LysThr: 2.789 ± 0.466
3.575LysVal: 3.575 ± 0.689
0.644LysTrp: 0.644 ± 0.186
1.144LysTyr: 1.144 ± 0.352
0.0LysXaa: 0.0 ± 0.0
Leu
8.58LeuAla: 8.58 ± 0.748
0.787LeuCys: 0.787 ± 0.263
6.221LeuAsp: 6.221 ± 0.626
6.292LeuGlu: 6.292 ± 0.779
2.36LeuPhe: 2.36 ± 0.416
6.078LeuGly: 6.078 ± 0.547
1.788LeuHis: 1.788 ± 0.353
3.79LeuIle: 3.79 ± 0.56
4.433LeuLys: 4.433 ± 0.534
6.292LeuLeu: 6.292 ± 0.785
2.074LeuMet: 2.074 ± 0.418
3.361LeuAsn: 3.361 ± 0.371
3.575LeuPro: 3.575 ± 0.443
5.22LeuGln: 5.22 ± 0.872
5.22LeuArg: 5.22 ± 0.809
7.293LeuSer: 7.293 ± 0.717
6.006LeuThr: 6.006 ± 0.65
4.934LeuVal: 4.934 ± 0.572
0.93LeuTrp: 0.93 ± 0.207
2.145LeuTyr: 2.145 ± 0.366
0.0LeuXaa: 0.0 ± 0.0
Met
3.003MetAla: 3.003 ± 0.537
0.286MetCys: 0.286 ± 0.156
1.359MetAsp: 1.359 ± 0.281
1.073MetGlu: 1.073 ± 0.278
1.144MetPhe: 1.144 ± 0.261
1.859MetGly: 1.859 ± 0.369
0.286MetHis: 0.286 ± 0.137
0.787MetIle: 0.787 ± 0.203
1.216MetLys: 1.216 ± 0.343
1.859MetLeu: 1.859 ± 0.358
0.715MetMet: 0.715 ± 0.255
0.93MetAsn: 0.93 ± 0.257
1.001MetPro: 1.001 ± 0.261
1.716MetGln: 1.716 ± 0.367
1.073MetArg: 1.073 ± 0.281
1.573MetSer: 1.573 ± 0.317
1.716MetThr: 1.716 ± 0.371
1.216MetVal: 1.216 ± 0.364
0.143MetTrp: 0.143 ± 0.103
0.286MetTyr: 0.286 ± 0.133
0.0MetXaa: 0.0 ± 0.0
Asn
3.718AsnAla: 3.718 ± 0.554
0.358AsnCys: 0.358 ± 0.153
1.788AsnAsp: 1.788 ± 0.358
2.074AsnGlu: 2.074 ± 0.531
1.573AsnPhe: 1.573 ± 0.387
2.717AsnGly: 2.717 ± 0.382
0.501AsnHis: 0.501 ± 0.176
1.931AsnIle: 1.931 ± 0.393
2.217AsnLys: 2.217 ± 0.382
4.576AsnLeu: 4.576 ± 0.551
1.216AsnMet: 1.216 ± 0.378
2.074AsnAsn: 2.074 ± 0.543
2.646AsnPro: 2.646 ± 0.6
2.002AsnGln: 2.002 ± 0.4
2.86AsnArg: 2.86 ± 0.443
2.717AsnSer: 2.717 ± 0.578
2.503AsnThr: 2.503 ± 0.413
2.932AsnVal: 2.932 ± 0.463
0.572AsnTrp: 0.572 ± 0.201
1.502AsnTyr: 1.502 ± 0.282
0.0AsnXaa: 0.0 ± 0.0
Pro
3.575ProAla: 3.575 ± 0.501
0.286ProCys: 0.286 ± 0.12
2.717ProAsp: 2.717 ± 0.364
3.289ProGlu: 3.289 ± 0.538
1.502ProPhe: 1.502 ± 0.384
3.933ProGly: 3.933 ± 0.542
0.572ProHis: 0.572 ± 0.204
2.002ProIle: 2.002 ± 0.387
2.145ProLys: 2.145 ± 0.449
3.146ProLeu: 3.146 ± 0.419
1.216ProMet: 1.216 ± 0.237
2.002ProAsn: 2.002 ± 0.441
1.216ProPro: 1.216 ± 0.257
1.931ProGln: 1.931 ± 0.439
1.645ProArg: 1.645 ± 0.356
3.933ProSer: 3.933 ± 0.441
2.789ProThr: 2.789 ± 0.41
3.361ProVal: 3.361 ± 0.457
0.787ProTrp: 0.787 ± 0.274
1.287ProTyr: 1.287 ± 0.353
0.0ProXaa: 0.0 ± 0.0
Gln
6.435GlnAla: 6.435 ± 0.864
0.215GlnCys: 0.215 ± 0.152
2.288GlnAsp: 2.288 ± 0.387
3.432GlnGlu: 3.432 ± 0.596
1.859GlnPhe: 1.859 ± 0.45
2.431GlnGly: 2.431 ± 0.604
1.144GlnHis: 1.144 ± 0.269
2.36GlnIle: 2.36 ± 0.37
1.931GlnLys: 1.931 ± 0.331
4.576GlnLeu: 4.576 ± 0.414
1.216GlnMet: 1.216 ± 0.245
1.931GlnAsn: 1.931 ± 0.519
1.645GlnPro: 1.645 ± 0.298
4.576GlnGln: 4.576 ± 0.955
2.86GlnArg: 2.86 ± 0.497
3.146GlnSer: 3.146 ± 0.481
2.717GlnThr: 2.717 ± 0.482
3.504GlnVal: 3.504 ± 0.446
0.93GlnTrp: 0.93 ± 0.297
2.002GlnTyr: 2.002 ± 0.502
0.0GlnXaa: 0.0 ± 0.0
Arg
5.577ArgAla: 5.577 ± 0.57
0.715ArgCys: 0.715 ± 0.315
2.503ArgAsp: 2.503 ± 0.456
2.86ArgGlu: 2.86 ± 0.484
2.002ArgPhe: 2.002 ± 0.329
3.289ArgGly: 3.289 ± 0.511
1.216ArgHis: 1.216 ± 0.37
3.575ArgIle: 3.575 ± 0.492
3.218ArgLys: 3.218 ± 0.496
5.577ArgLeu: 5.577 ± 0.556
1.144ArgMet: 1.144 ± 0.273
2.431ArgAsn: 2.431 ± 0.397
1.859ArgPro: 1.859 ± 0.362
3.003ArgGln: 3.003 ± 0.45
2.932ArgArg: 2.932 ± 0.473
3.432ArgSer: 3.432 ± 0.349
2.86ArgThr: 2.86 ± 0.552
4.505ArgVal: 4.505 ± 0.501
0.644ArgTrp: 0.644 ± 0.182
1.645ArgTyr: 1.645 ± 0.336
0.0ArgXaa: 0.0 ± 0.0
Ser
6.364SerAla: 6.364 ± 0.947
0.93SerCys: 0.93 ± 0.264
3.361SerAsp: 3.361 ± 0.47
2.932SerGlu: 2.932 ± 0.502
2.002SerPhe: 2.002 ± 0.35
6.507SerGly: 6.507 ± 0.943
0.787SerHis: 0.787 ± 0.26
2.717SerIle: 2.717 ± 0.553
2.789SerLys: 2.789 ± 0.456
5.506SerLeu: 5.506 ± 0.6
1.216SerMet: 1.216 ± 0.321
3.075SerAsn: 3.075 ± 0.398
3.218SerPro: 3.218 ± 0.438
3.146SerGln: 3.146 ± 0.452
4.147SerArg: 4.147 ± 0.522
3.361SerSer: 3.361 ± 0.46
3.647SerThr: 3.647 ± 0.649
4.791SerVal: 4.791 ± 0.606
0.858SerTrp: 0.858 ± 0.22
1.931SerTyr: 1.931 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
6.435ThrAla: 6.435 ± 1.065
0.429ThrCys: 0.429 ± 0.173
4.934ThrAsp: 4.934 ± 0.535
3.504ThrGlu: 3.504 ± 0.463
2.288ThrPhe: 2.288 ± 0.355
5.935ThrGly: 5.935 ± 0.702
0.715ThrHis: 0.715 ± 0.267
3.075ThrIle: 3.075 ± 0.478
3.504ThrLys: 3.504 ± 0.429
4.004ThrLeu: 4.004 ± 0.526
1.645ThrMet: 1.645 ± 0.317
3.218ThrAsn: 3.218 ± 0.64
3.575ThrPro: 3.575 ± 0.503
1.502ThrGln: 1.502 ± 0.275
2.789ThrArg: 2.789 ± 0.427
3.504ThrSer: 3.504 ± 0.728
3.146ThrThr: 3.146 ± 0.545
4.505ThrVal: 4.505 ± 0.683
1.001ThrTrp: 1.001 ± 0.273
2.431ThrTyr: 2.431 ± 0.445
0.0ThrXaa: 0.0 ± 0.0
Val
6.578ValAla: 6.578 ± 0.728
0.501ValCys: 0.501 ± 0.191
4.648ValAsp: 4.648 ± 0.75
5.077ValGlu: 5.077 ± 0.64
2.074ValPhe: 2.074 ± 0.336
6.006ValGly: 6.006 ± 0.626
0.715ValHis: 0.715 ± 0.231
2.717ValIle: 2.717 ± 0.408
2.86ValLys: 2.86 ± 0.504
5.291ValLeu: 5.291 ± 0.631
2.145ValMet: 2.145 ± 0.447
3.003ValAsn: 3.003 ± 0.426
1.859ValPro: 1.859 ± 0.278
3.146ValGln: 3.146 ± 0.376
3.075ValArg: 3.075 ± 0.319
4.505ValSer: 4.505 ± 0.773
4.505ValThr: 4.505 ± 0.569
5.22ValVal: 5.22 ± 0.713
1.144ValTrp: 1.144 ± 0.287
2.217ValTyr: 2.217 ± 0.392
0.0ValXaa: 0.0 ± 0.0
Trp
1.359TrpAla: 1.359 ± 0.335
0.143TrpCys: 0.143 ± 0.113
0.93TrpAsp: 0.93 ± 0.212
0.858TrpGlu: 0.858 ± 0.302
0.501TrpPhe: 0.501 ± 0.201
0.715TrpGly: 0.715 ± 0.293
0.358TrpHis: 0.358 ± 0.134
0.572TrpIle: 0.572 ± 0.235
0.787TrpLys: 0.787 ± 0.215
1.573TrpLeu: 1.573 ± 0.322
0.429TrpMet: 0.429 ± 0.164
0.787TrpAsn: 0.787 ± 0.254
0.429TrpPro: 0.429 ± 0.168
0.787TrpGln: 0.787 ± 0.203
0.715TrpArg: 0.715 ± 0.255
1.144TrpSer: 1.144 ± 0.324
0.286TrpThr: 0.286 ± 0.113
1.073TrpVal: 1.073 ± 0.274
0.143TrpTrp: 0.143 ± 0.093
0.501TrpTyr: 0.501 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.217TyrAla: 2.217 ± 0.471
0.572TyrCys: 0.572 ± 0.271
1.645TyrAsp: 1.645 ± 0.274
1.573TyrGlu: 1.573 ± 0.37
1.073TyrPhe: 1.073 ± 0.335
3.218TyrGly: 3.218 ± 0.509
0.572TyrHis: 0.572 ± 0.212
1.573TyrIle: 1.573 ± 0.365
1.859TyrLys: 1.859 ± 0.408
2.503TyrLeu: 2.503 ± 0.332
0.501TyrMet: 0.501 ± 0.156
1.359TyrAsn: 1.359 ± 0.316
0.787TyrPro: 0.787 ± 0.197
1.645TyrGln: 1.645 ± 0.468
2.145TyrArg: 2.145 ± 0.42
2.145TyrSer: 2.145 ± 0.38
2.36TyrThr: 2.36 ± 0.466
1.788TyrVal: 1.788 ± 0.333
0.572TyrTrp: 0.572 ± 0.201
0.93TyrTyr: 0.93 ± 0.194
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (13987 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski