Amino acid dipepetide frequency for Stx2-converting phage 1717

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.137AlaAla: 10.137 ± 1.544
1.086AlaCys: 1.086 ± 0.308
5.793AlaAsp: 5.793 ± 0.68
7.844AlaGlu: 7.844 ± 0.753
3.077AlaPhe: 3.077 ± 0.459
6.818AlaGly: 6.818 ± 0.828
1.388AlaHis: 1.388 ± 0.294
5.008AlaIle: 5.008 ± 0.472
4.224AlaLys: 4.224 ± 0.485
8.447AlaLeu: 8.447 ± 0.865
2.957AlaMet: 2.957 ± 0.393
3.56AlaAsn: 3.56 ± 0.465
2.353AlaPro: 2.353 ± 0.389
3.5AlaGln: 3.5 ± 0.505
6.275AlaArg: 6.275 ± 0.76
6.456AlaSer: 6.456 ± 0.783
3.982AlaThr: 3.982 ± 0.479
6.758AlaVal: 6.758 ± 0.883
1.689AlaTrp: 1.689 ± 0.306
2.414AlaTyr: 2.414 ± 0.395
0.0AlaXaa: 0.0 ± 0.0
Cys
0.784CysAla: 0.784 ± 0.237
0.543CysCys: 0.543 ± 0.168
0.845CysAsp: 0.845 ± 0.247
0.784CysGlu: 0.784 ± 0.262
0.362CysPhe: 0.362 ± 0.145
1.146CysGly: 1.146 ± 0.267
0.483CysHis: 0.483 ± 0.163
0.784CysIle: 0.784 ± 0.242
0.603CysLys: 0.603 ± 0.179
1.267CysLeu: 1.267 ± 0.283
0.302CysMet: 0.302 ± 0.142
0.664CysAsn: 0.664 ± 0.175
0.603CysPro: 0.603 ± 0.215
0.302CysGln: 0.302 ± 0.13
1.267CysArg: 1.267 ± 0.327
1.146CysSer: 1.146 ± 0.262
1.026CysThr: 1.026 ± 0.269
0.965CysVal: 0.965 ± 0.234
0.241CysTrp: 0.241 ± 0.103
0.362CysTyr: 0.362 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
5.008AspAla: 5.008 ± 0.579
0.302AspCys: 0.302 ± 0.129
3.439AspAsp: 3.439 ± 0.508
3.62AspGlu: 3.62 ± 0.476
1.75AspPhe: 1.75 ± 0.281
5.793AspGly: 5.793 ± 0.614
0.845AspHis: 0.845 ± 0.209
3.681AspIle: 3.681 ± 0.398
2.957AspLys: 2.957 ± 0.428
3.681AspLeu: 3.681 ± 0.598
1.75AspMet: 1.75 ± 0.31
2.414AspAsn: 2.414 ± 0.361
1.931AspPro: 1.931 ± 0.361
1.207AspGln: 1.207 ± 0.305
3.439AspArg: 3.439 ± 0.343
2.957AspSer: 2.957 ± 0.39
2.896AspThr: 2.896 ± 0.524
4.043AspVal: 4.043 ± 0.601
1.086AspTrp: 1.086 ± 0.353
2.052AspTyr: 2.052 ± 0.453
0.0AspXaa: 0.0 ± 0.0
Glu
6.336GluAla: 6.336 ± 0.747
1.086GluCys: 1.086 ± 0.277
2.534GluAsp: 2.534 ± 0.359
3.681GluGlu: 3.681 ± 0.577
2.776GluPhe: 2.776 ± 0.416
3.439GluGly: 3.439 ± 0.449
1.267GluHis: 1.267 ± 0.266
4.525GluIle: 4.525 ± 0.527
4.344GluLys: 4.344 ± 0.467
6.094GluLeu: 6.094 ± 0.656
2.052GluMet: 2.052 ± 0.32
2.595GluAsn: 2.595 ± 0.368
1.991GluPro: 1.991 ± 0.355
4.103GluGln: 4.103 ± 0.486
4.887GluArg: 4.887 ± 0.575
4.224GluSer: 4.224 ± 0.569
2.896GluThr: 2.896 ± 0.509
3.319GluVal: 3.319 ± 0.523
0.845GluTrp: 0.845 ± 0.201
1.931GluTyr: 1.931 ± 0.342
0.0GluXaa: 0.0 ± 0.0
Phe
2.353PheAla: 2.353 ± 0.373
0.543PheCys: 0.543 ± 0.174
1.81PheAsp: 1.81 ± 0.283
1.508PheGlu: 1.508 ± 0.272
0.784PhePhe: 0.784 ± 0.228
2.353PheGly: 2.353 ± 0.331
0.724PheHis: 0.724 ± 0.185
1.448PheIle: 1.448 ± 0.361
1.569PheLys: 1.569 ± 0.366
2.172PheLeu: 2.172 ± 0.32
1.026PheMet: 1.026 ± 0.29
1.569PheAsn: 1.569 ± 0.273
1.026PhePro: 1.026 ± 0.249
0.664PheGln: 0.664 ± 0.182
3.017PheArg: 3.017 ± 0.375
3.801PheSer: 3.801 ± 0.505
2.112PheThr: 2.112 ± 0.381
2.414PheVal: 2.414 ± 0.318
0.483PheTrp: 0.483 ± 0.15
0.905PheTyr: 0.905 ± 0.212
0.0PheXaa: 0.0 ± 0.0
Gly
5.551GlyAla: 5.551 ± 0.691
0.905GlyCys: 0.905 ± 0.222
4.586GlyAsp: 4.586 ± 0.556
4.767GlyGlu: 4.767 ± 0.577
2.776GlyPhe: 2.776 ± 0.433
5.31GlyGly: 5.31 ± 0.703
1.448GlyHis: 1.448 ± 0.288
4.103GlyIle: 4.103 ± 0.408
4.163GlyLys: 4.163 ± 0.45
5.068GlyLeu: 5.068 ± 0.506
2.896GlyMet: 2.896 ± 0.443
3.62GlyAsn: 3.62 ± 0.392
2.353GlyPro: 2.353 ± 1.04
3.077GlyGln: 3.077 ± 0.496
4.706GlyArg: 4.706 ± 0.518
4.646GlySer: 4.646 ± 0.477
3.198GlyThr: 3.198 ± 0.498
5.31GlyVal: 5.31 ± 0.558
1.689GlyTrp: 1.689 ± 0.272
1.871GlyTyr: 1.871 ± 0.36
0.0GlyXaa: 0.0 ± 0.0
His
1.508HisAla: 1.508 ± 0.327
0.422HisCys: 0.422 ± 0.159
1.448HisAsp: 1.448 ± 0.296
1.207HisGlu: 1.207 ± 0.302
0.784HisPhe: 0.784 ± 0.239
2.172HisGly: 2.172 ± 0.431
0.905HisHis: 0.905 ± 0.23
1.267HisIle: 1.267 ± 0.286
0.965HisLys: 0.965 ± 0.238
1.508HisLeu: 1.508 ± 0.29
0.121HisMet: 0.121 ± 0.082
0.543HisAsn: 0.543 ± 0.162
1.086HisPro: 1.086 ± 0.262
0.784HisGln: 0.784 ± 0.221
1.267HisArg: 1.267 ± 0.285
1.026HisSer: 1.026 ± 0.243
1.267HisThr: 1.267 ± 0.206
1.146HisVal: 1.146 ± 0.198
0.362HisTrp: 0.362 ± 0.139
0.483HisTyr: 0.483 ± 0.156
0.0HisXaa: 0.0 ± 0.0
Ile
5.491IleAla: 5.491 ± 0.546
1.086IleCys: 1.086 ± 0.236
3.681IleAsp: 3.681 ± 0.439
3.138IleGlu: 3.138 ± 0.471
0.965IlePhe: 0.965 ± 0.181
3.077IleGly: 3.077 ± 0.291
1.327IleHis: 1.327 ± 0.28
2.474IleIle: 2.474 ± 0.368
2.776IleLys: 2.776 ± 0.32
3.138IleLeu: 3.138 ± 0.474
0.965IleMet: 0.965 ± 0.252
2.776IleAsn: 2.776 ± 0.463
2.414IlePro: 2.414 ± 0.493
2.112IleGln: 2.112 ± 0.318
4.103IleArg: 4.103 ± 0.467
5.431IleSer: 5.431 ± 0.717
4.344IleThr: 4.344 ± 0.417
1.991IleVal: 1.991 ± 0.333
0.724IleTrp: 0.724 ± 0.211
1.146IleTyr: 1.146 ± 0.323
0.0IleXaa: 0.0 ± 0.0
Lys
5.551LysAla: 5.551 ± 0.544
0.664LysCys: 0.664 ± 0.189
2.474LysAsp: 2.474 ± 0.366
3.62LysGlu: 3.62 ± 0.558
1.146LysPhe: 1.146 ± 0.268
3.982LysGly: 3.982 ± 0.529
0.965LysHis: 0.965 ± 0.251
2.474LysIle: 2.474 ± 0.414
3.439LysLys: 3.439 ± 0.62
4.284LysLeu: 4.284 ± 0.53
1.75LysMet: 1.75 ± 0.389
2.414LysAsn: 2.414 ± 0.412
3.017LysPro: 3.017 ± 0.378
2.836LysGln: 2.836 ± 0.48
3.258LysArg: 3.258 ± 0.532
3.319LysSer: 3.319 ± 0.431
3.862LysThr: 3.862 ± 0.506
3.077LysVal: 3.077 ± 0.519
0.905LysTrp: 0.905 ± 0.234
1.267LysTyr: 1.267 ± 0.292
0.0LysXaa: 0.0 ± 0.0
Leu
9.232LeuAla: 9.232 ± 0.758
1.388LeuCys: 1.388 ± 0.325
3.258LeuAsp: 3.258 ± 0.4
4.767LeuGlu: 4.767 ± 0.599
1.871LeuPhe: 1.871 ± 0.285
3.862LeuGly: 3.862 ± 0.548
1.81LeuHis: 1.81 ± 0.366
4.405LeuIle: 4.405 ± 0.603
5.37LeuLys: 5.37 ± 0.581
6.396LeuLeu: 6.396 ± 0.727
2.112LeuMet: 2.112 ± 0.299
4.103LeuAsn: 4.103 ± 0.448
3.801LeuPro: 3.801 ± 0.476
3.56LeuGln: 3.56 ± 0.521
6.698LeuArg: 6.698 ± 0.609
5.853LeuSer: 5.853 ± 0.66
5.732LeuThr: 5.732 ± 0.628
3.62LeuVal: 3.62 ± 0.488
2.112LeuTrp: 2.112 ± 0.345
2.233LeuTyr: 2.233 ± 0.328
0.0LeuXaa: 0.0 ± 0.0
Met
2.836MetAla: 2.836 ± 0.416
0.241MetCys: 0.241 ± 0.12
1.508MetAsp: 1.508 ± 0.317
1.146MetGlu: 1.146 ± 0.279
0.724MetPhe: 0.724 ± 0.212
1.388MetGly: 1.388 ± 0.239
0.181MetHis: 0.181 ± 0.095
1.146MetIle: 1.146 ± 0.256
1.81MetLys: 1.81 ± 0.38
2.595MetLeu: 2.595 ± 0.468
0.543MetMet: 0.543 ± 0.16
1.448MetAsn: 1.448 ± 0.237
0.965MetPro: 0.965 ± 0.203
1.629MetGln: 1.629 ± 0.275
1.871MetArg: 1.871 ± 0.293
2.595MetSer: 2.595 ± 0.431
2.534MetThr: 2.534 ± 0.358
1.146MetVal: 1.146 ± 0.324
0.483MetTrp: 0.483 ± 0.148
0.302MetTyr: 0.302 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
4.284AsnAla: 4.284 ± 0.503
0.664AsnCys: 0.664 ± 0.18
2.233AsnAsp: 2.233 ± 0.331
2.715AsnGlu: 2.715 ± 0.497
0.965AsnPhe: 0.965 ± 0.273
4.284AsnGly: 4.284 ± 0.531
1.327AsnHis: 1.327 ± 0.265
2.595AsnIle: 2.595 ± 0.38
2.776AsnLys: 2.776 ± 0.396
3.198AsnLeu: 3.198 ± 0.441
0.603AsnMet: 0.603 ± 0.164
1.81AsnAsn: 1.81 ± 0.383
2.293AsnPro: 2.293 ± 0.333
1.267AsnGln: 1.267 ± 0.242
2.595AsnArg: 2.595 ± 0.419
2.655AsnSer: 2.655 ± 0.381
2.595AsnThr: 2.595 ± 0.545
2.715AsnVal: 2.715 ± 0.395
0.483AsnTrp: 0.483 ± 0.156
1.146AsnTyr: 1.146 ± 0.249
0.0AsnXaa: 0.0 ± 0.0
Pro
3.862ProAla: 3.862 ± 0.623
0.422ProCys: 0.422 ± 0.156
3.198ProAsp: 3.198 ± 0.643
4.043ProGlu: 4.043 ± 0.474
1.629ProPhe: 1.629 ± 0.282
3.138ProGly: 3.138 ± 0.44
0.664ProHis: 0.664 ± 0.164
1.388ProIle: 1.388 ± 0.302
2.112ProLys: 2.112 ± 0.423
2.112ProLeu: 2.112 ± 0.33
0.965ProMet: 0.965 ± 0.303
1.086ProAsn: 1.086 ± 0.296
2.172ProPro: 2.172 ± 0.471
1.871ProGln: 1.871 ± 0.356
1.931ProArg: 1.931 ± 0.387
3.017ProSer: 3.017 ± 0.302
1.75ProThr: 1.75 ± 0.269
4.525ProVal: 4.525 ± 0.67
0.603ProTrp: 0.603 ± 0.197
0.845ProTyr: 0.845 ± 0.218
0.0ProXaa: 0.0 ± 0.0
Gln
3.5GlnAla: 3.5 ± 0.615
1.026GlnCys: 1.026 ± 0.227
1.448GlnAsp: 1.448 ± 0.279
2.776GlnGlu: 2.776 ± 0.419
1.689GlnPhe: 1.689 ± 0.29
2.715GlnGly: 2.715 ± 0.389
1.086GlnHis: 1.086 ± 0.223
2.957GlnIle: 2.957 ± 0.362
2.836GlnLys: 2.836 ± 0.458
3.5GlnLeu: 3.5 ± 0.481
1.388GlnMet: 1.388 ± 0.374
1.689GlnAsn: 1.689 ± 0.446
1.75GlnPro: 1.75 ± 0.357
2.595GlnGln: 2.595 ± 0.569
3.017GlnArg: 3.017 ± 0.512
2.957GlnSer: 2.957 ± 0.49
1.931GlnThr: 1.931 ± 0.313
2.112GlnVal: 2.112 ± 0.366
0.845GlnTrp: 0.845 ± 0.187
1.388GlnTyr: 1.388 ± 0.322
0.0GlnXaa: 0.0 ± 0.0
Arg
4.706ArgAla: 4.706 ± 0.692
0.784ArgCys: 0.784 ± 0.205
3.922ArgAsp: 3.922 ± 0.679
4.948ArgGlu: 4.948 ± 0.513
2.414ArgPhe: 2.414 ± 0.436
4.284ArgGly: 4.284 ± 0.438
1.75ArgHis: 1.75 ± 0.308
3.56ArgIle: 3.56 ± 0.559
4.405ArgLys: 4.405 ± 0.631
7.241ArgLeu: 7.241 ± 0.606
2.172ArgMet: 2.172 ± 0.344
3.801ArgAsn: 3.801 ± 0.498
2.293ArgPro: 2.293 ± 0.343
3.439ArgGln: 3.439 ± 0.39
6.094ArgArg: 6.094 ± 0.689
3.258ArgSer: 3.258 ± 0.447
3.017ArgThr: 3.017 ± 0.375
4.405ArgVal: 4.405 ± 0.423
1.026ArgTrp: 1.026 ± 0.253
1.569ArgTyr: 1.569 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
7.361SerAla: 7.361 ± 0.782
0.965SerCys: 0.965 ± 0.261
3.62SerAsp: 3.62 ± 0.502
4.525SerGlu: 4.525 ± 0.454
1.991SerPhe: 1.991 ± 0.381
6.094SerGly: 6.094 ± 0.6
0.965SerHis: 0.965 ± 0.269
3.56SerIle: 3.56 ± 0.516
1.931SerLys: 1.931 ± 0.415
6.215SerLeu: 6.215 ± 0.545
2.052SerMet: 2.052 ± 0.32
2.655SerAsn: 2.655 ± 0.345
3.077SerPro: 3.077 ± 0.385
3.922SerGln: 3.922 ± 0.444
4.344SerArg: 4.344 ± 0.53
4.344SerSer: 4.344 ± 0.457
3.379SerThr: 3.379 ± 0.485
4.344SerVal: 4.344 ± 0.576
0.784SerTrp: 0.784 ± 0.204
2.233SerTyr: 2.233 ± 0.394
0.0SerXaa: 0.0 ± 0.0
Thr
5.551ThrAla: 5.551 ± 0.739
1.086ThrCys: 1.086 ± 0.302
3.138ThrAsp: 3.138 ± 0.525
4.163ThrGlu: 4.163 ± 0.667
2.293ThrPhe: 2.293 ± 0.416
5.431ThrGly: 5.431 ± 0.684
1.086ThrHis: 1.086 ± 0.209
2.172ThrIle: 2.172 ± 0.328
2.534ThrLys: 2.534 ± 0.304
5.672ThrLeu: 5.672 ± 0.559
0.905ThrMet: 0.905 ± 0.25
1.508ThrAsn: 1.508 ± 0.257
3.379ThrPro: 3.379 ± 0.523
2.112ThrGln: 2.112 ± 0.297
2.595ThrArg: 2.595 ± 0.341
3.017ThrSer: 3.017 ± 0.439
3.439ThrThr: 3.439 ± 0.46
4.827ThrVal: 4.827 ± 0.63
0.483ThrTrp: 0.483 ± 0.152
1.689ThrTyr: 1.689 ± 0.359
0.0ThrXaa: 0.0 ± 0.0
Val
5.913ValAla: 5.913 ± 0.712
0.905ValCys: 0.905 ± 0.229
3.138ValAsp: 3.138 ± 0.331
3.801ValGlu: 3.801 ± 0.435
1.81ValPhe: 1.81 ± 0.338
3.922ValGly: 3.922 ± 0.566
0.784ValHis: 0.784 ± 0.232
3.56ValIle: 3.56 ± 0.546
3.439ValLys: 3.439 ± 0.428
5.793ValLeu: 5.793 ± 0.551
1.508ValMet: 1.508 ± 0.258
3.62ValAsn: 3.62 ± 0.44
2.957ValPro: 2.957 ± 0.466
2.052ValGln: 2.052 ± 0.438
3.982ValArg: 3.982 ± 0.463
4.948ValSer: 4.948 ± 0.594
4.525ValThr: 4.525 ± 0.66
4.344ValVal: 4.344 ± 0.524
1.207ValTrp: 1.207 ± 0.265
1.991ValTyr: 1.991 ± 0.304
0.0ValXaa: 0.0 ± 0.0
Trp
1.207TrpAla: 1.207 ± 0.219
0.302TrpCys: 0.302 ± 0.128
0.845TrpAsp: 0.845 ± 0.228
0.543TrpGlu: 0.543 ± 0.188
0.724TrpPhe: 0.724 ± 0.223
0.905TrpGly: 0.905 ± 0.237
0.543TrpHis: 0.543 ± 0.194
0.664TrpIle: 0.664 ± 0.145
0.845TrpLys: 0.845 ± 0.203
1.871TrpLeu: 1.871 ± 0.368
0.422TrpMet: 0.422 ± 0.157
0.543TrpAsn: 0.543 ± 0.17
0.845TrpPro: 0.845 ± 0.204
0.845TrpGln: 0.845 ± 0.167
1.629TrpArg: 1.629 ± 0.244
0.784TrpSer: 0.784 ± 0.201
1.026TrpThr: 1.026 ± 0.217
1.569TrpVal: 1.569 ± 0.237
0.241TrpTrp: 0.241 ± 0.145
0.603TrpTyr: 0.603 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.655TyrAla: 2.655 ± 0.356
0.06TyrCys: 0.06 ± 0.049
1.931TyrAsp: 1.931 ± 0.334
1.327TyrGlu: 1.327 ± 0.297
1.75TyrPhe: 1.75 ± 0.294
1.931TyrGly: 1.931 ± 0.275
0.724TyrHis: 0.724 ± 0.143
1.388TyrIle: 1.388 ± 0.299
1.207TyrLys: 1.207 ± 0.418
1.931TyrLeu: 1.931 ± 0.295
0.543TyrMet: 0.543 ± 0.182
0.784TyrAsn: 0.784 ± 0.217
1.026TyrPro: 1.026 ± 0.208
1.327TyrGln: 1.327 ± 0.346
2.112TyrArg: 2.112 ± 0.364
1.931TyrSer: 1.931 ± 0.329
1.569TyrThr: 1.569 ± 0.286
1.569TyrVal: 1.569 ± 0.326
0.664TyrTrp: 0.664 ± 0.192
0.784TyrTyr: 0.784 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (16574 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski