Amino acid dipepetide frequency for Pectobacterium phage PPWS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.319AlaAla: 11.319 ± 0.995
0.871AlaCys: 0.871 ± 0.227
6.893AlaAsp: 6.893 ± 0.743
5.66AlaGlu: 5.66 ± 0.778
3.483AlaPhe: 3.483 ± 0.491
7.111AlaGly: 7.111 ± 0.727
2.757AlaHis: 2.757 ± 0.535
4.426AlaIle: 4.426 ± 0.505
3.773AlaLys: 3.773 ± 0.585
9.287AlaLeu: 9.287 ± 1.026
2.177AlaMet: 2.177 ± 0.362
3.555AlaAsn: 3.555 ± 0.573
3.193AlaPro: 3.193 ± 0.527
4.499AlaGln: 4.499 ± 0.521
5.079AlaArg: 5.079 ± 0.818
5.514AlaSer: 5.514 ± 0.559
5.732AlaThr: 5.732 ± 0.768
6.82AlaVal: 6.82 ± 0.624
0.871AlaTrp: 0.871 ± 0.226
3.7AlaTyr: 3.7 ± 0.548
0.0AlaXaa: 0.0 ± 0.0
Cys
0.653CysAla: 0.653 ± 0.236
0.073CysCys: 0.073 ± 0.079
0.943CysAsp: 0.943 ± 0.359
0.58CysGlu: 0.58 ± 0.198
0.435CysPhe: 0.435 ± 0.19
0.798CysGly: 0.798 ± 0.312
0.29CysHis: 0.29 ± 0.129
0.726CysIle: 0.726 ± 0.253
0.145CysLys: 0.145 ± 0.101
0.798CysLeu: 0.798 ± 0.286
0.508CysMet: 0.508 ± 0.228
0.435CysAsn: 0.435 ± 0.183
0.508CysPro: 0.508 ± 0.198
0.29CysGln: 0.29 ± 0.141
0.363CysArg: 0.363 ± 0.156
0.435CysSer: 0.435 ± 0.203
0.798CysThr: 0.798 ± 0.262
0.871CysVal: 0.871 ± 0.278
0.363CysTrp: 0.363 ± 0.178
0.798CysTyr: 0.798 ± 0.304
0.0CysXaa: 0.0 ± 0.0
Asp
6.675AspAla: 6.675 ± 0.647
0.726AspCys: 0.726 ± 0.258
4.136AspAsp: 4.136 ± 0.679
3.193AspGlu: 3.193 ± 0.466
1.741AspPhe: 1.741 ± 0.429
4.499AspGly: 4.499 ± 0.624
0.871AspHis: 0.871 ± 0.266
3.628AspIle: 3.628 ± 0.456
2.902AspLys: 2.902 ± 0.464
5.007AspLeu: 5.007 ± 0.628
2.322AspMet: 2.322 ± 0.386
3.12AspAsn: 3.12 ± 0.447
2.032AspPro: 2.032 ± 0.448
1.088AspGln: 1.088 ± 0.253
2.83AspArg: 2.83 ± 0.528
4.499AspSer: 4.499 ± 0.472
5.007AspThr: 5.007 ± 0.629
6.095AspVal: 6.095 ± 0.64
1.233AspTrp: 1.233 ± 0.292
2.322AspTyr: 2.322 ± 0.497
0.0AspXaa: 0.0 ± 0.0
Glu
4.789GluAla: 4.789 ± 0.635
0.58GluCys: 0.58 ± 0.284
2.612GluAsp: 2.612 ± 0.375
3.483GluGlu: 3.483 ± 0.574
2.685GluPhe: 2.685 ± 0.362
3.338GluGly: 3.338 ± 0.403
1.306GluHis: 1.306 ± 0.33
1.887GluIle: 1.887 ± 0.32
1.814GluLys: 1.814 ± 0.44
4.499GluLeu: 4.499 ± 0.699
1.814GluMet: 1.814 ± 0.38
2.394GluAsn: 2.394 ± 0.421
1.088GluPro: 1.088 ± 0.282
3.193GluGln: 3.193 ± 0.425
3.483GluArg: 3.483 ± 0.517
3.047GluSer: 3.047 ± 0.421
2.975GluThr: 2.975 ± 0.509
3.41GluVal: 3.41 ± 0.539
0.58GluTrp: 0.58 ± 0.239
2.467GluTyr: 2.467 ± 0.305
0.0GluXaa: 0.0 ± 0.0
Phe
2.685PheAla: 2.685 ± 0.446
0.145PheCys: 0.145 ± 0.116
2.975PheAsp: 2.975 ± 0.448
1.088PheGlu: 1.088 ± 0.255
1.306PhePhe: 1.306 ± 0.263
3.338PheGly: 3.338 ± 0.433
0.58PheHis: 0.58 ± 0.221
1.088PheIle: 1.088 ± 0.266
1.596PheLys: 1.596 ± 0.323
1.959PheLeu: 1.959 ± 0.349
0.508PheMet: 0.508 ± 0.167
1.596PheAsn: 1.596 ± 0.336
1.596PhePro: 1.596 ± 0.348
0.943PheGln: 0.943 ± 0.236
1.814PheArg: 1.814 ± 0.418
1.959PheSer: 1.959 ± 0.451
1.669PheThr: 1.669 ± 0.341
2.177PheVal: 2.177 ± 0.425
0.363PheTrp: 0.363 ± 0.148
0.58PheTyr: 0.58 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
7.546GlyAla: 7.546 ± 0.757
1.451GlyCys: 1.451 ± 0.397
4.789GlyAsp: 4.789 ± 0.655
2.54GlyGlu: 2.54 ± 0.417
2.467GlyPhe: 2.467 ± 0.345
5.587GlyGly: 5.587 ± 0.868
0.943GlyHis: 0.943 ± 0.227
5.079GlyIle: 5.079 ± 0.493
3.846GlyLys: 3.846 ± 0.624
5.587GlyLeu: 5.587 ± 0.648
2.685GlyMet: 2.685 ± 0.472
3.193GlyAsn: 3.193 ± 0.454
1.887GlyPro: 1.887 ± 0.402
3.047GlyGln: 3.047 ± 0.503
3.483GlyArg: 3.483 ± 0.552
4.861GlySer: 4.861 ± 0.821
6.24GlyThr: 6.24 ± 0.826
6.313GlyVal: 6.313 ± 0.681
0.871GlyTrp: 0.871 ± 0.287
3.555GlyTyr: 3.555 ± 0.682
0.0GlyXaa: 0.0 ± 0.0
His
1.524HisAla: 1.524 ± 0.352
0.435HisCys: 0.435 ± 0.181
1.596HisAsp: 1.596 ± 0.381
1.016HisGlu: 1.016 ± 0.317
0.435HisPhe: 0.435 ± 0.167
2.249HisGly: 2.249 ± 0.568
0.726HisHis: 0.726 ± 0.287
0.943HisIle: 0.943 ± 0.253
0.726HisLys: 0.726 ± 0.274
2.249HisLeu: 2.249 ± 0.433
0.58HisMet: 0.58 ± 0.223
1.161HisAsn: 1.161 ± 0.338
1.161HisPro: 1.161 ± 0.367
0.871HisGln: 0.871 ± 0.296
1.451HisArg: 1.451 ± 0.349
1.379HisSer: 1.379 ± 0.401
0.653HisThr: 0.653 ± 0.22
1.741HisVal: 1.741 ± 0.406
0.435HisTrp: 0.435 ± 0.187
0.871HisTyr: 0.871 ± 0.305
0.0HisXaa: 0.0 ± 0.0
Ile
4.934IleAla: 4.934 ± 0.554
0.435IleCys: 0.435 ± 0.199
2.902IleAsp: 2.902 ± 0.437
3.265IleGlu: 3.265 ± 0.48
0.508IlePhe: 0.508 ± 0.244
3.265IleGly: 3.265 ± 0.5
1.161IleHis: 1.161 ± 0.309
1.669IleIle: 1.669 ± 0.411
2.394IleLys: 2.394 ± 0.424
4.063IleLeu: 4.063 ± 0.628
1.233IleMet: 1.233 ± 0.233
2.612IleAsn: 2.612 ± 0.424
2.249IlePro: 2.249 ± 0.392
1.741IleGln: 1.741 ± 0.432
2.612IleArg: 2.612 ± 0.462
3.265IleSer: 3.265 ± 0.42
3.846IleThr: 3.846 ± 0.585
2.612IleVal: 2.612 ± 0.387
0.435IleTrp: 0.435 ± 0.191
1.088IleTyr: 1.088 ± 0.258
0.0IleXaa: 0.0 ± 0.0
Lys
4.716LysAla: 4.716 ± 0.749
0.435LysCys: 0.435 ± 0.203
2.685LysAsp: 2.685 ± 0.36
2.467LysGlu: 2.467 ± 0.454
0.871LysPhe: 0.871 ± 0.27
2.612LysGly: 2.612 ± 0.513
0.943LysHis: 0.943 ± 0.321
1.524LysIle: 1.524 ± 0.323
1.524LysLys: 1.524 ± 0.55
4.571LysLeu: 4.571 ± 0.457
1.088LysMet: 1.088 ± 0.356
1.161LysAsn: 1.161 ± 0.293
1.959LysPro: 1.959 ± 0.297
2.54LysGln: 2.54 ± 0.492
2.104LysArg: 2.104 ± 0.375
1.814LysSer: 1.814 ± 0.502
2.177LysThr: 2.177 ± 0.478
2.902LysVal: 2.902 ± 0.394
0.435LysTrp: 0.435 ± 0.237
1.887LysTyr: 1.887 ± 0.484
0.0LysXaa: 0.0 ± 0.0
Leu
7.836LeuAla: 7.836 ± 0.759
1.379LeuCys: 1.379 ± 0.379
5.007LeuAsp: 5.007 ± 0.587
4.789LeuGlu: 4.789 ± 0.531
2.104LeuPhe: 2.104 ± 0.407
6.385LeuGly: 6.385 ± 0.8
2.032LeuHis: 2.032 ± 0.446
3.628LeuIle: 3.628 ± 0.49
3.41LeuLys: 3.41 ± 0.528
7.619LeuLeu: 7.619 ± 0.865
2.394LeuMet: 2.394 ± 0.373
3.846LeuAsn: 3.846 ± 0.49
4.499LeuPro: 4.499 ± 0.469
3.265LeuGln: 3.265 ± 0.58
6.675LeuArg: 6.675 ± 0.888
6.53LeuSer: 6.53 ± 0.891
5.297LeuThr: 5.297 ± 0.666
7.038LeuVal: 7.038 ± 0.62
0.943LeuTrp: 0.943 ± 0.244
3.12LeuTyr: 3.12 ± 0.513
0.0LeuXaa: 0.0 ± 0.0
Met
2.902MetAla: 2.902 ± 0.546
0.218MetCys: 0.218 ± 0.137
1.233MetAsp: 1.233 ± 0.356
1.306MetGlu: 1.306 ± 0.299
1.524MetPhe: 1.524 ± 0.426
1.887MetGly: 1.887 ± 0.374
0.726MetHis: 0.726 ± 0.22
0.726MetIle: 0.726 ± 0.212
1.306MetLys: 1.306 ± 0.334
2.54MetLeu: 2.54 ± 0.5
0.58MetMet: 0.58 ± 0.165
0.726MetAsn: 0.726 ± 0.246
1.596MetPro: 1.596 ± 0.438
1.814MetGln: 1.814 ± 0.415
2.322MetArg: 2.322 ± 0.492
1.233MetSer: 1.233 ± 0.288
1.669MetThr: 1.669 ± 0.344
2.249MetVal: 2.249 ± 0.359
0.145MetTrp: 0.145 ± 0.097
1.524MetTyr: 1.524 ± 0.296
0.0MetXaa: 0.0 ± 0.0
Asn
3.7AsnAla: 3.7 ± 0.66
0.29AsnCys: 0.29 ± 0.133
1.524AsnAsp: 1.524 ± 0.303
1.959AsnGlu: 1.959 ± 0.442
1.741AsnPhe: 1.741 ± 0.326
3.7AsnGly: 3.7 ± 0.642
0.798AsnHis: 0.798 ± 0.237
2.104AsnIle: 2.104 ± 0.416
2.612AsnLys: 2.612 ± 0.336
3.773AsnLeu: 3.773 ± 0.522
1.669AsnMet: 1.669 ± 0.361
1.524AsnAsn: 1.524 ± 0.363
1.887AsnPro: 1.887 ± 0.526
1.669AsnGln: 1.669 ± 0.646
2.322AsnArg: 2.322 ± 0.37
3.265AsnSer: 3.265 ± 0.599
2.975AsnThr: 2.975 ± 0.527
2.83AsnVal: 2.83 ± 0.447
0.726AsnTrp: 0.726 ± 0.29
0.871AsnTyr: 0.871 ± 0.292
0.0AsnXaa: 0.0 ± 0.0
Pro
4.136ProAla: 4.136 ± 0.64
0.218ProCys: 0.218 ± 0.139
3.265ProAsp: 3.265 ± 0.545
2.902ProGlu: 2.902 ± 0.393
0.653ProPhe: 0.653 ± 0.215
1.959ProGly: 1.959 ± 0.382
0.508ProHis: 0.508 ± 0.168
1.814ProIle: 1.814 ± 0.455
1.379ProLys: 1.379 ± 0.294
3.12ProLeu: 3.12 ± 0.471
1.379ProMet: 1.379 ± 0.246
1.959ProAsn: 1.959 ± 0.433
1.959ProPro: 1.959 ± 0.564
1.451ProGln: 1.451 ± 0.411
2.394ProArg: 2.394 ± 0.371
2.83ProSer: 2.83 ± 0.365
3.773ProThr: 3.773 ± 0.583
3.193ProVal: 3.193 ± 0.404
0.435ProTrp: 0.435 ± 0.194
1.669ProTyr: 1.669 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
4.063GlnAla: 4.063 ± 0.879
0.218GlnCys: 0.218 ± 0.111
2.104GlnAsp: 2.104 ± 0.478
2.757GlnGlu: 2.757 ± 0.447
1.669GlnPhe: 1.669 ± 0.319
4.571GlnGly: 4.571 ± 0.72
1.669GlnHis: 1.669 ± 0.467
2.322GlnIle: 2.322 ± 0.364
1.161GlnLys: 1.161 ± 0.282
3.773GlnLeu: 3.773 ± 0.569
1.451GlnMet: 1.451 ± 0.359
1.741GlnAsn: 1.741 ± 0.595
1.088GlnPro: 1.088 ± 0.352
2.757GlnGln: 2.757 ± 0.78
3.12GlnArg: 3.12 ± 0.51
2.54GlnSer: 2.54 ± 0.588
2.249GlnThr: 2.249 ± 0.347
2.685GlnVal: 2.685 ± 0.521
0.58GlnTrp: 0.58 ± 0.193
2.322GlnTyr: 2.322 ± 0.42
0.0GlnXaa: 0.0 ± 0.0
Arg
5.079ArgAla: 5.079 ± 0.662
0.726ArgCys: 0.726 ± 0.267
3.918ArgAsp: 3.918 ± 0.502
2.902ArgGlu: 2.902 ± 0.504
1.161ArgPhe: 1.161 ± 0.34
4.861ArgGly: 4.861 ± 0.81
1.524ArgHis: 1.524 ± 0.327
2.975ArgIle: 2.975 ± 0.541
2.249ArgLys: 2.249 ± 0.421
4.644ArgLeu: 4.644 ± 0.607
1.379ArgMet: 1.379 ± 0.299
2.902ArgAsn: 2.902 ± 0.518
1.814ArgPro: 1.814 ± 0.272
2.902ArgGln: 2.902 ± 0.511
4.861ArgArg: 4.861 ± 0.513
4.499ArgSer: 4.499 ± 0.63
3.41ArgThr: 3.41 ± 0.571
4.499ArgVal: 4.499 ± 0.519
0.871ArgTrp: 0.871 ± 0.292
2.54ArgTyr: 2.54 ± 0.459
0.0ArgXaa: 0.0 ± 0.0
Ser
6.603SerAla: 6.603 ± 0.685
0.435SerCys: 0.435 ± 0.188
4.644SerAsp: 4.644 ± 0.59
2.757SerGlu: 2.757 ± 0.388
1.524SerPhe: 1.524 ± 0.337
6.022SerGly: 6.022 ± 0.693
0.871SerHis: 0.871 ± 0.248
3.555SerIle: 3.555 ± 0.475
3.483SerLys: 3.483 ± 0.508
6.385SerLeu: 6.385 ± 0.713
1.596SerMet: 1.596 ± 0.357
2.467SerAsn: 2.467 ± 0.509
2.394SerPro: 2.394 ± 0.538
2.612SerGln: 2.612 ± 0.484
3.047SerArg: 3.047 ± 0.436
4.208SerSer: 4.208 ± 0.585
4.861SerThr: 4.861 ± 0.871
4.934SerVal: 4.934 ± 0.572
1.016SerTrp: 1.016 ± 0.288
1.741SerTyr: 1.741 ± 0.353
0.0SerXaa: 0.0 ± 0.0
Thr
7.619ThrAla: 7.619 ± 0.69
0.58ThrCys: 0.58 ± 0.244
4.208ThrAsp: 4.208 ± 0.522
2.757ThrGlu: 2.757 ± 0.49
1.814ThrPhe: 1.814 ± 0.407
5.297ThrGly: 5.297 ± 0.807
1.887ThrHis: 1.887 ± 0.335
3.12ThrIle: 3.12 ± 0.475
1.887ThrLys: 1.887 ± 0.406
5.224ThrLeu: 5.224 ± 0.711
0.943ThrMet: 0.943 ± 0.282
2.394ThrAsn: 2.394 ± 0.343
3.265ThrPro: 3.265 ± 0.492
2.612ThrGln: 2.612 ± 0.366
2.685ThrArg: 2.685 ± 0.406
4.281ThrSer: 4.281 ± 0.706
5.442ThrThr: 5.442 ± 1.282
7.038ThrVal: 7.038 ± 0.734
1.088ThrTrp: 1.088 ± 0.249
2.104ThrTyr: 2.104 ± 0.412
0.0ThrXaa: 0.0 ± 0.0
Val
6.675ValAla: 6.675 ± 0.638
1.161ValCys: 1.161 ± 0.324
4.426ValAsp: 4.426 ± 0.455
3.628ValGlu: 3.628 ± 0.526
2.104ValPhe: 2.104 ± 0.374
5.732ValGly: 5.732 ± 0.517
1.524ValHis: 1.524 ± 0.305
2.612ValIle: 2.612 ± 0.442
2.612ValLys: 2.612 ± 0.563
7.183ValLeu: 7.183 ± 0.794
2.032ValMet: 2.032 ± 0.412
3.047ValAsn: 3.047 ± 0.486
3.918ValPro: 3.918 ± 0.506
5.007ValGln: 5.007 ± 0.758
5.587ValArg: 5.587 ± 0.81
5.079ValSer: 5.079 ± 0.873
4.426ValThr: 4.426 ± 0.756
5.152ValVal: 5.152 ± 0.672
0.871ValTrp: 0.871 ± 0.234
3.12ValTyr: 3.12 ± 0.488
0.0ValXaa: 0.0 ± 0.0
Trp
0.726TrpAla: 0.726 ± 0.231
0.218TrpCys: 0.218 ± 0.118
0.943TrpAsp: 0.943 ± 0.264
0.798TrpGlu: 0.798 ± 0.323
0.726TrpPhe: 0.726 ± 0.31
0.726TrpGly: 0.726 ± 0.21
0.218TrpHis: 0.218 ± 0.124
0.218TrpIle: 0.218 ± 0.126
0.363TrpLys: 0.363 ± 0.148
1.379TrpLeu: 1.379 ± 0.372
0.363TrpMet: 0.363 ± 0.154
0.363TrpAsn: 0.363 ± 0.131
0.508TrpPro: 0.508 ± 0.214
0.943TrpGln: 0.943 ± 0.29
0.943TrpArg: 0.943 ± 0.266
0.58TrpSer: 0.58 ± 0.211
0.58TrpThr: 0.58 ± 0.191
1.161TrpVal: 1.161 ± 0.283
0.145TrpTrp: 0.145 ± 0.099
1.016TrpTyr: 1.016 ± 0.272
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.54TyrAla: 2.54 ± 0.369
0.218TyrCys: 0.218 ± 0.134
3.12TyrAsp: 3.12 ± 0.479
1.596TyrGlu: 1.596 ± 0.352
1.016TyrPhe: 1.016 ± 0.298
2.032TyrGly: 2.032 ± 0.365
0.871TyrHis: 0.871 ± 0.231
2.249TyrIle: 2.249 ± 0.448
1.233TyrLys: 1.233 ± 0.272
4.136TyrLeu: 4.136 ± 0.774
1.451TyrMet: 1.451 ± 0.344
1.741TyrAsn: 1.741 ± 0.395
2.394TyrPro: 2.394 ± 0.322
1.596TyrGln: 1.596 ± 0.322
2.612TyrArg: 2.612 ± 0.406
3.338TyrSer: 3.338 ± 0.654
2.467TyrThr: 2.467 ± 0.508
2.177TyrVal: 2.177 ± 0.363
0.508TyrTrp: 0.508 ± 0.227
1.233TyrTyr: 1.233 ± 0.466
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (13783 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski