Amino acid dipepetide frequency for Mycobacterium phage Florinda

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.373AlaAla: 14.373 ± 1.742
0.997AlaCys: 0.997 ± 0.216
7.816AlaAsp: 7.816 ± 0.691
6.924AlaGlu: 6.924 ± 0.629
3.2AlaPhe: 3.2 ± 0.452
10.072AlaGly: 10.072 ± 1.298
2.256AlaHis: 2.256 ± 0.393
4.354AlaIle: 4.354 ± 0.543
3.829AlaLys: 3.829 ± 0.411
8.026AlaLeu: 8.026 ± 0.836
2.623AlaMet: 2.623 ± 0.443
2.57AlaAsn: 2.57 ± 0.389
4.931AlaPro: 4.931 ± 0.62
3.777AlaGln: 3.777 ± 0.474
7.869AlaArg: 7.869 ± 0.724
5.088AlaSer: 5.088 ± 0.587
5.613AlaThr: 5.613 ± 0.499
6.452AlaVal: 6.452 ± 0.71
2.938AlaTrp: 2.938 ± 0.543
2.518AlaTyr: 2.518 ± 0.432
0.0AlaXaa: 0.0 ± 0.0
Cys
0.944CysAla: 0.944 ± 0.234
0.21CysCys: 0.21 ± 0.147
1.364CysAsp: 1.364 ± 0.342
0.944CysGlu: 0.944 ± 0.275
0.157CysPhe: 0.157 ± 0.104
2.151CysGly: 2.151 ± 0.526
0.157CysHis: 0.157 ± 0.088
0.367CysIle: 0.367 ± 0.136
0.472CysLys: 0.472 ± 0.17
0.525CysLeu: 0.525 ± 0.191
0.262CysMet: 0.262 ± 0.104
0.367CysAsn: 0.367 ± 0.152
1.259CysPro: 1.259 ± 0.302
0.315CysGln: 0.315 ± 0.13
0.734CysArg: 0.734 ± 0.271
0.472CysSer: 0.472 ± 0.167
0.734CysThr: 0.734 ± 0.232
0.629CysVal: 0.629 ± 0.17
0.367CysTrp: 0.367 ± 0.123
0.262CysTyr: 0.262 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
7.029AspAla: 7.029 ± 0.589
1.207AspCys: 1.207 ± 0.251
4.721AspAsp: 4.721 ± 0.504
3.724AspGlu: 3.724 ± 0.468
1.836AspPhe: 1.836 ± 0.247
6.977AspGly: 6.977 ± 0.661
1.311AspHis: 1.311 ± 0.242
2.256AspIle: 2.256 ± 0.365
1.731AspLys: 1.731 ± 0.262
5.98AspLeu: 5.98 ± 0.555
1.207AspMet: 1.207 ± 0.258
2.046AspAsn: 2.046 ± 0.409
4.983AspPro: 4.983 ± 0.519
2.413AspGln: 2.413 ± 0.36
5.246AspArg: 5.246 ± 0.618
3.41AspSer: 3.41 ± 0.48
4.144AspThr: 4.144 ± 0.498
4.406AspVal: 4.406 ± 0.576
1.364AspTrp: 1.364 ± 0.277
2.046AspTyr: 2.046 ± 0.341
0.0AspXaa: 0.0 ± 0.0
Glu
6.767GluAla: 6.767 ± 0.649
0.734GluCys: 0.734 ± 0.238
2.99GluAsp: 2.99 ± 0.374
2.938GluGlu: 2.938 ± 0.452
1.993GluPhe: 1.993 ± 0.389
4.092GluGly: 4.092 ± 0.449
1.626GluHis: 1.626 ± 0.347
1.679GluIle: 1.679 ± 0.276
1.941GluLys: 1.941 ± 0.307
5.456GluLeu: 5.456 ± 0.526
1.784GluMet: 1.784 ± 0.306
1.784GluAsn: 1.784 ± 0.231
3.043GluPro: 3.043 ± 0.481
2.728GluGln: 2.728 ± 0.407
4.197GluArg: 4.197 ± 0.56
2.885GluSer: 2.885 ± 0.461
4.197GluThr: 4.197 ± 0.561
3.934GluVal: 3.934 ± 0.485
1.416GluTrp: 1.416 ± 0.267
1.521GluTyr: 1.521 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.78PheAla: 2.78 ± 0.347
0.21PheCys: 0.21 ± 0.122
2.361PheAsp: 2.361 ± 0.396
1.521PheGlu: 1.521 ± 0.298
0.944PhePhe: 0.944 ± 0.268
2.938PheGly: 2.938 ± 0.676
0.42PheHis: 0.42 ± 0.135
1.364PheIle: 1.364 ± 0.303
0.892PheLys: 0.892 ± 0.209
1.679PheLeu: 1.679 ± 0.294
0.787PheMet: 0.787 ± 0.226
0.997PheAsn: 0.997 ± 0.36
1.836PhePro: 1.836 ± 0.329
0.944PheGln: 0.944 ± 0.302
1.784PheArg: 1.784 ± 0.287
1.731PheSer: 1.731 ± 0.303
2.151PheThr: 2.151 ± 0.331
2.151PheVal: 2.151 ± 0.359
0.42PheTrp: 0.42 ± 0.13
0.997PheTyr: 0.997 ± 0.296
0.0PheXaa: 0.0 ± 0.0
Gly
9.128GlyAla: 9.128 ± 1.147
1.259GlyCys: 1.259 ± 0.271
6.347GlyAsp: 6.347 ± 0.516
3.829GlyGlu: 3.829 ± 0.396
2.833GlyPhe: 2.833 ± 0.423
11.278GlyGly: 11.278 ± 1.803
1.784GlyHis: 1.784 ± 0.311
4.302GlyIle: 4.302 ± 0.538
2.833GlyLys: 2.833 ± 0.378
5.665GlyLeu: 5.665 ± 0.542
2.99GlyMet: 2.99 ± 0.543
2.78GlyAsn: 2.78 ± 0.355
4.092GlyPro: 4.092 ± 0.603
2.57GlyGln: 2.57 ± 0.581
5.561GlyArg: 5.561 ± 0.689
6.4GlySer: 6.4 ± 0.845
6.662GlyThr: 6.662 ± 0.854
5.875GlyVal: 5.875 ± 0.623
2.675GlyTrp: 2.675 ± 0.455
2.098GlyTyr: 2.098 ± 0.383
0.0GlyXaa: 0.0 ± 0.0
His
1.888HisAla: 1.888 ± 0.391
0.367HisCys: 0.367 ± 0.185
1.154HisAsp: 1.154 ± 0.228
1.311HisGlu: 1.311 ± 0.256
0.577HisPhe: 0.577 ± 0.161
1.311HisGly: 1.311 ± 0.29
0.892HisHis: 0.892 ± 0.24
1.469HisIle: 1.469 ± 0.315
0.629HisLys: 0.629 ± 0.182
1.207HisLeu: 1.207 ± 0.245
0.472HisMet: 0.472 ± 0.131
0.944HisAsn: 0.944 ± 0.252
1.731HisPro: 1.731 ± 0.293
0.787HisGln: 0.787 ± 0.216
2.256HisArg: 2.256 ± 0.399
0.839HisSer: 0.839 ± 0.22
1.574HisThr: 1.574 ± 0.355
1.259HisVal: 1.259 ± 0.26
0.42HisTrp: 0.42 ± 0.122
0.892HisTyr: 0.892 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
5.036IleAla: 5.036 ± 0.482
0.577IleCys: 0.577 ± 0.211
3.829IleAsp: 3.829 ± 0.506
3.724IleGlu: 3.724 ± 0.403
0.629IlePhe: 0.629 ± 0.2
3.41IleGly: 3.41 ± 0.489
1.469IleHis: 1.469 ± 0.307
1.154IleIle: 1.154 ± 0.25
1.102IleLys: 1.102 ± 0.253
2.203IleLeu: 2.203 ± 0.416
0.472IleMet: 0.472 ± 0.168
1.941IleAsn: 1.941 ± 0.313
2.938IlePro: 2.938 ± 0.346
1.259IleGln: 1.259 ± 0.233
2.466IleArg: 2.466 ± 0.384
2.098IleSer: 2.098 ± 0.462
3.672IleThr: 3.672 ± 0.466
2.99IleVal: 2.99 ± 0.333
0.892IleTrp: 0.892 ± 0.25
0.787IleTyr: 0.787 ± 0.195
0.0IleXaa: 0.0 ± 0.0
Lys
3.829LysAla: 3.829 ± 0.533
0.42LysCys: 0.42 ± 0.168
1.364LysAsp: 1.364 ± 0.277
1.469LysGlu: 1.469 ± 0.28
1.364LysPhe: 1.364 ± 0.241
2.57LysGly: 2.57 ± 0.417
1.102LysHis: 1.102 ± 0.238
0.944LysIle: 0.944 ± 0.248
1.521LysLys: 1.521 ± 0.409
2.308LysLeu: 2.308 ± 0.43
0.472LysMet: 0.472 ± 0.163
0.997LysAsn: 0.997 ± 0.239
2.466LysPro: 2.466 ± 0.489
1.521LysGln: 1.521 ± 0.283
2.256LysArg: 2.256 ± 0.377
2.203LysSer: 2.203 ± 0.311
2.151LysThr: 2.151 ± 0.332
2.256LysVal: 2.256 ± 0.392
1.154LysTrp: 1.154 ± 0.292
0.892LysTyr: 0.892 ± 0.264
0.0LysXaa: 0.0 ± 0.0
Leu
7.239LeuAla: 7.239 ± 0.877
0.892LeuCys: 0.892 ± 0.21
5.298LeuAsp: 5.298 ± 0.541
3.724LeuGlu: 3.724 ± 0.433
1.941LeuPhe: 1.941 ± 0.272
5.508LeuGly: 5.508 ± 0.59
1.102LeuHis: 1.102 ± 0.303
3.043LeuIle: 3.043 ± 0.376
2.098LeuLys: 2.098 ± 0.321
4.774LeuLeu: 4.774 ± 0.568
1.521LeuMet: 1.521 ± 0.313
2.046LeuAsn: 2.046 ± 0.332
5.77LeuPro: 5.77 ± 0.681
2.728LeuGln: 2.728 ± 0.463
5.351LeuArg: 5.351 ± 0.653
4.616LeuSer: 4.616 ± 0.513
5.351LeuThr: 5.351 ± 0.569
5.298LeuVal: 5.298 ± 0.541
1.311LeuTrp: 1.311 ± 0.303
2.203LeuTyr: 2.203 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
2.361MetAla: 2.361 ± 0.396
0.472MetCys: 0.472 ± 0.25
1.364MetAsp: 1.364 ± 0.304
0.944MetGlu: 0.944 ± 0.169
0.682MetPhe: 0.682 ± 0.21
1.784MetGly: 1.784 ± 0.256
0.105MetHis: 0.105 ± 0.076
0.997MetIle: 0.997 ± 0.255
1.207MetLys: 1.207 ± 0.273
1.574MetLeu: 1.574 ± 0.19
0.629MetMet: 0.629 ± 0.222
1.259MetAsn: 1.259 ± 0.266
1.469MetPro: 1.469 ± 0.239
0.577MetGln: 0.577 ± 0.138
1.679MetArg: 1.679 ± 0.313
2.675MetSer: 2.675 ± 0.399
1.836MetThr: 1.836 ± 0.292
1.049MetVal: 1.049 ± 0.3
0.262MetTrp: 0.262 ± 0.094
0.42MetTyr: 0.42 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.62AsnAla: 3.62 ± 0.369
0.21AsnCys: 0.21 ± 0.095
1.731AsnAsp: 1.731 ± 0.301
1.626AsnGlu: 1.626 ± 0.343
0.734AsnPhe: 0.734 ± 0.278
4.354AsnGly: 4.354 ± 0.555
0.734AsnHis: 0.734 ± 0.169
1.521AsnIle: 1.521 ± 0.41
0.944AsnLys: 0.944 ± 0.236
2.203AsnLeu: 2.203 ± 0.343
0.682AsnMet: 0.682 ± 0.178
1.941AsnAsn: 1.941 ± 0.356
2.57AsnPro: 2.57 ± 0.366
1.154AsnGln: 1.154 ± 0.314
2.151AsnArg: 2.151 ± 0.37
1.888AsnSer: 1.888 ± 0.324
2.623AsnThr: 2.623 ± 0.345
2.098AsnVal: 2.098 ± 0.347
0.682AsnTrp: 0.682 ± 0.18
0.525AsnTyr: 0.525 ± 0.149
0.0AsnXaa: 0.0 ± 0.0
Pro
5.508ProAla: 5.508 ± 0.594
0.734ProCys: 0.734 ± 0.188
4.774ProAsp: 4.774 ± 0.531
3.934ProGlu: 3.934 ± 0.4
1.888ProPhe: 1.888 ± 0.373
6.347ProGly: 6.347 ± 0.671
1.416ProHis: 1.416 ± 0.297
2.046ProIle: 2.046 ± 0.283
2.203ProLys: 2.203 ± 0.356
4.302ProLeu: 4.302 ± 0.554
1.521ProMet: 1.521 ± 0.32
2.518ProAsn: 2.518 ± 0.345
3.829ProPro: 3.829 ± 0.622
2.046ProGln: 2.046 ± 0.337
3.515ProArg: 3.515 ± 0.412
3.462ProSer: 3.462 ± 0.414
3.515ProThr: 3.515 ± 0.457
4.354ProVal: 4.354 ± 0.454
1.364ProTrp: 1.364 ± 0.237
1.259ProTyr: 1.259 ± 0.217
0.0ProXaa: 0.0 ± 0.0
Gln
4.879GlnAla: 4.879 ± 0.653
0.262GlnCys: 0.262 ± 0.11
1.836GlnAsp: 1.836 ± 0.263
1.941GlnGlu: 1.941 ± 0.313
1.102GlnPhe: 1.102 ± 0.223
2.256GlnGly: 2.256 ± 0.488
0.892GlnHis: 0.892 ± 0.249
1.731GlnIle: 1.731 ± 0.297
1.259GlnLys: 1.259 ± 0.259
2.675GlnLeu: 2.675 ± 0.378
0.629GlnMet: 0.629 ± 0.169
0.839GlnAsn: 0.839 ± 0.286
2.57GlnPro: 2.57 ± 0.412
1.259GlnGln: 1.259 ± 0.337
2.78GlnArg: 2.78 ± 0.399
1.993GlnSer: 1.993 ± 0.357
1.941GlnThr: 1.941 ± 0.304
2.256GlnVal: 2.256 ± 0.357
0.525GlnTrp: 0.525 ± 0.163
0.839GlnTyr: 0.839 ± 0.263
0.0GlnXaa: 0.0 ± 0.0
Arg
7.187ArgAla: 7.187 ± 0.746
1.311ArgCys: 1.311 ± 0.406
4.459ArgAsp: 4.459 ± 0.528
4.511ArgGlu: 4.511 ± 0.554
2.098ArgPhe: 2.098 ± 0.349
4.564ArgGly: 4.564 ± 0.528
1.626ArgHis: 1.626 ± 0.349
3.777ArgIle: 3.777 ± 0.552
2.466ArgLys: 2.466 ± 0.405
5.508ArgLeu: 5.508 ± 0.589
2.308ArgMet: 2.308 ± 0.41
2.78ArgAsn: 2.78 ± 0.413
3.41ArgPro: 3.41 ± 0.371
2.046ArgGln: 2.046 ± 0.398
5.98ArgArg: 5.98 ± 0.861
3.829ArgSer: 3.829 ± 0.489
3.252ArgThr: 3.252 ± 0.43
4.774ArgVal: 4.774 ± 0.611
1.888ArgTrp: 1.888 ± 0.355
1.836ArgTyr: 1.836 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
5.823SerAla: 5.823 ± 0.816
0.629SerCys: 0.629 ± 0.223
4.197SerAsp: 4.197 ± 0.518
3.095SerGlu: 3.095 ± 0.395
1.679SerPhe: 1.679 ± 0.333
6.662SerGly: 6.662 ± 0.896
1.102SerHis: 1.102 ± 0.243
2.675SerIle: 2.675 ± 0.43
2.203SerLys: 2.203 ± 0.373
3.724SerLeu: 3.724 ± 0.42
1.259SerMet: 1.259 ± 0.218
2.203SerAsn: 2.203 ± 0.39
3.252SerPro: 3.252 ± 0.346
1.574SerGln: 1.574 ± 0.233
3.252SerArg: 3.252 ± 0.326
3.567SerSer: 3.567 ± 0.637
3.567SerThr: 3.567 ± 0.498
4.774SerVal: 4.774 ± 0.683
1.521SerTrp: 1.521 ± 0.272
1.049SerTyr: 1.049 ± 0.239
0.0SerXaa: 0.0 ± 0.0
Thr
6.347ThrAla: 6.347 ± 0.597
0.577ThrCys: 0.577 ± 0.208
3.829ThrAsp: 3.829 ± 0.547
3.252ThrGlu: 3.252 ± 0.326
1.731ThrPhe: 1.731 ± 0.325
6.505ThrGly: 6.505 ± 0.636
1.836ThrHis: 1.836 ± 0.364
3.62ThrIle: 3.62 ± 0.439
2.256ThrLys: 2.256 ± 0.351
4.511ThrLeu: 4.511 ± 0.513
1.102ThrMet: 1.102 ± 0.239
2.361ThrAsn: 2.361 ± 0.411
4.302ThrPro: 4.302 ± 0.43
2.256ThrGln: 2.256 ± 0.311
4.039ThrArg: 4.039 ± 0.542
3.777ThrSer: 3.777 ± 0.494
5.036ThrThr: 5.036 ± 0.594
6.242ThrVal: 6.242 ± 0.59
1.154ThrTrp: 1.154 ± 0.289
1.731ThrTyr: 1.731 ± 0.317
0.0ThrXaa: 0.0 ± 0.0
Val
6.977ValAla: 6.977 ± 0.606
0.997ValCys: 0.997 ± 0.23
5.246ValAsp: 5.246 ± 0.56
5.036ValGlu: 5.036 ± 0.628
2.256ValPhe: 2.256 ± 0.431
5.088ValGly: 5.088 ± 0.709
1.154ValHis: 1.154 ± 0.241
2.78ValIle: 2.78 ± 0.397
2.308ValLys: 2.308 ± 0.392
5.875ValLeu: 5.875 ± 0.673
1.416ValMet: 1.416 ± 0.235
2.151ValAsn: 2.151 ± 0.302
3.724ValPro: 3.724 ± 0.305
2.518ValGln: 2.518 ± 0.384
4.092ValArg: 4.092 ± 0.495
4.616ValSer: 4.616 ± 0.606
5.088ValThr: 5.088 ± 0.505
6.4ValVal: 6.4 ± 0.668
1.836ValTrp: 1.836 ± 0.32
1.626ValTyr: 1.626 ± 0.323
0.0ValXaa: 0.0 ± 0.0
Trp
1.836TrpAla: 1.836 ± 0.288
0.262TrpCys: 0.262 ± 0.145
1.626TrpAsp: 1.626 ± 0.266
1.469TrpGlu: 1.469 ± 0.318
0.734TrpPhe: 0.734 ± 0.231
0.892TrpGly: 0.892 ± 0.2
0.682TrpHis: 0.682 ± 0.196
1.259TrpIle: 1.259 ± 0.246
0.577TrpLys: 0.577 ± 0.164
1.836TrpLeu: 1.836 ± 0.312
1.049TrpMet: 1.049 ± 0.228
0.734TrpAsn: 0.734 ± 0.208
0.997TrpPro: 0.997 ± 0.271
1.049TrpGln: 1.049 ± 0.305
2.466TrpArg: 2.466 ± 0.444
1.207TrpSer: 1.207 ± 0.303
1.731TrpThr: 1.731 ± 0.326
1.888TrpVal: 1.888 ± 0.402
0.839TrpTrp: 0.839 ± 0.168
0.525TrpTyr: 0.525 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.675TyrAla: 2.675 ± 0.388
0.367TyrCys: 0.367 ± 0.173
1.731TyrAsp: 1.731 ± 0.398
1.836TyrGlu: 1.836 ± 0.336
0.525TyrPhe: 0.525 ± 0.182
2.203TyrGly: 2.203 ± 0.486
0.315TyrHis: 0.315 ± 0.109
1.311TyrIle: 1.311 ± 0.242
0.787TyrLys: 0.787 ± 0.237
1.784TyrLeu: 1.784 ± 0.341
0.105TyrMet: 0.105 ± 0.062
0.734TyrAsn: 0.734 ± 0.183
1.364TyrPro: 1.364 ± 0.243
0.944TyrGln: 0.944 ± 0.192
1.941TyrArg: 1.941 ± 0.321
1.049TyrSer: 1.049 ± 0.24
1.626TyrThr: 1.626 ± 0.346
2.098TyrVal: 2.098 ± 0.332
0.629TyrTrp: 0.629 ± 0.192
0.682TyrTyr: 0.682 ± 0.147
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 117 proteins (19064 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski