Amino acid dipepetide frequency for bacterium JGI 053

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.826AlaAla: 21.826 ± 0.401
1.155AlaCys: 1.155 ± 0.059
7.082AlaAsp: 7.082 ± 0.153
9.041AlaGlu: 9.041 ± 0.222
4.07AlaPhe: 4.07 ± 0.095
12.807AlaGly: 12.807 ± 0.241
2.475AlaHis: 2.475 ± 0.081
4.347AlaIle: 4.347 ± 0.126
2.281AlaLys: 2.281 ± 0.076
14.268AlaLeu: 14.268 ± 0.293
2.713AlaMet: 2.713 ± 0.082
2.375AlaAsn: 2.375 ± 0.085
7.32AlaPro: 7.32 ± 0.191
3.85AlaGln: 3.85 ± 0.117
11.55AlaArg: 11.55 ± 0.256
5.84AlaSer: 5.84 ± 0.136
6.131AlaThr: 6.131 ± 0.164
10.882AlaVal: 10.882 ± 0.206
1.896AlaTrp: 1.896 ± 0.091
2.632AlaTyr: 2.632 ± 0.089
0.0AlaXaa: 0.0 ± 0.0
Cys
0.998CysAla: 0.998 ± 0.055
0.123CysCys: 0.123 ± 0.019
0.498CysAsp: 0.498 ± 0.04
0.375CysGlu: 0.375 ± 0.033
0.259CysPhe: 0.259 ± 0.023
0.961CysGly: 0.961 ± 0.06
0.165CysHis: 0.165 ± 0.022
0.306CysIle: 0.306 ± 0.028
0.139CysLys: 0.139 ± 0.023
0.555CysLeu: 0.555 ± 0.04
0.147CysMet: 0.147 ± 0.018
0.233CysAsn: 0.233 ± 0.032
0.511CysPro: 0.511 ± 0.061
0.178CysGln: 0.178 ± 0.021
0.621CysArg: 0.621 ± 0.039
0.477CysSer: 0.477 ± 0.032
0.443CysThr: 0.443 ± 0.05
0.631CysVal: 0.631 ± 0.046
0.155CysTrp: 0.155 ± 0.031
0.181CysTyr: 0.181 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
7.836AspAla: 7.836 ± 0.196
0.377AspCys: 0.377 ± 0.035
3.444AspAsp: 3.444 ± 0.126
3.91AspGlu: 3.91 ± 0.119
1.946AspPhe: 1.946 ± 0.081
5.241AspGly: 5.241 ± 0.132
1.008AspHis: 1.008 ± 0.062
1.543AspIle: 1.543 ± 0.061
0.691AspLys: 0.691 ± 0.048
5.248AspLeu: 5.248 ± 0.112
0.741AspMet: 0.741 ± 0.043
0.814AspAsn: 0.814 ± 0.047
4.025AspPro: 4.025 ± 0.11
1.333AspGln: 1.333 ± 0.056
4.62AspArg: 4.62 ± 0.115
2.669AspSer: 2.669 ± 0.12
2.635AspThr: 2.635 ± 0.086
4.837AspVal: 4.837 ± 0.125
0.89AspTrp: 0.89 ± 0.047
1.278AspTyr: 1.278 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
8.072GluAla: 8.072 ± 0.191
0.43GluCys: 0.43 ± 0.037
2.564GluAsp: 2.564 ± 0.089
3.701GluGlu: 3.701 ± 0.146
1.739GluPhe: 1.739 ± 0.071
4.691GluGly: 4.691 ± 0.132
1.501GluHis: 1.501 ± 0.069
2.624GluIle: 2.624 ± 0.101
1.524GluLys: 1.524 ± 0.081
6.854GluLeu: 6.854 ± 0.187
1.443GluMet: 1.443 ± 0.067
0.998GluAsn: 0.998 ± 0.056
3.357GluPro: 3.357 ± 0.109
1.938GluGln: 1.938 ± 0.079
6.945GluArg: 6.945 ± 0.182
2.54GluSer: 2.54 ± 0.088
3.072GluThr: 3.072 ± 0.092
5.238GluVal: 5.238 ± 0.158
1.0GluTrp: 1.0 ± 0.052
1.414GluTyr: 1.414 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
4.245PheAla: 4.245 ± 0.127
0.348PheCys: 0.348 ± 0.03
2.158PheAsp: 2.158 ± 0.068
1.844PheGlu: 1.844 ± 0.075
1.247PhePhe: 1.247 ± 0.072
3.365PheGly: 3.365 ± 0.109
0.762PheHis: 0.762 ± 0.048
0.964PheIle: 0.964 ± 0.052
0.563PheLys: 0.563 ± 0.042
2.931PheLeu: 2.931 ± 0.084
0.524PheMet: 0.524 ± 0.044
0.867PheAsn: 0.867 ± 0.053
1.634PhePro: 1.634 ± 0.072
0.935PheGln: 0.935 ± 0.046
2.844PheArg: 2.844 ± 0.101
1.901PheSer: 1.901 ± 0.078
1.988PheThr: 1.988 ± 0.076
2.957PheVal: 2.957 ± 0.101
0.487PheTrp: 0.487 ± 0.037
0.909PheTyr: 0.909 ± 0.063
0.0PheXaa: 0.0 ± 0.0
Gly
10.934GlyAla: 10.934 ± 0.183
0.835GlyCys: 0.835 ± 0.067
5.183GlyAsp: 5.183 ± 0.116
5.751GlyGlu: 5.751 ± 0.136
3.266GlyPhe: 3.266 ± 0.097
9.554GlyGly: 9.554 ± 0.27
1.849GlyHis: 1.849 ± 0.081
3.986GlyIle: 3.986 ± 0.107
2.365GlyLys: 2.365 ± 0.08
7.519GlyLeu: 7.519 ± 0.155
2.394GlyMet: 2.394 ± 0.079
1.841GlyAsn: 1.841 ± 0.084
3.905GlyPro: 3.905 ± 0.121
2.402GlyGln: 2.402 ± 0.085
7.938GlyArg: 7.938 ± 0.16
4.774GlySer: 4.774 ± 0.139
5.843GlyThr: 5.843 ± 0.171
7.446GlyVal: 7.446 ± 0.148
1.757GlyTrp: 1.757 ± 0.075
2.436GlyTyr: 2.436 ± 0.083
0.0GlyXaa: 0.0 ± 0.0
His
2.771HisAla: 2.771 ± 0.103
0.186HisCys: 0.186 ± 0.025
1.289HisAsp: 1.289 ± 0.063
1.058HisGlu: 1.058 ± 0.052
0.691HisPhe: 0.691 ± 0.043
2.038HisGly: 2.038 ± 0.081
0.49HisHis: 0.49 ± 0.04
0.55HisIle: 0.55 ± 0.041
0.265HisLys: 0.265 ± 0.029
2.242HisLeu: 2.242 ± 0.075
0.291HisMet: 0.291 ± 0.029
0.375HisAsn: 0.375 ± 0.036
1.616HisPro: 1.616 ± 0.069
0.55HisGln: 0.55 ± 0.038
1.763HisArg: 1.763 ± 0.071
0.93HisSer: 0.93 ± 0.05
0.904HisThr: 0.904 ± 0.048
1.776HisVal: 1.776 ± 0.075
0.317HisTrp: 0.317 ± 0.031
0.553HisTyr: 0.553 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
4.748IleAla: 4.748 ± 0.114
0.285IleCys: 0.285 ± 0.027
2.412IleAsp: 2.412 ± 0.077
2.354IleGlu: 2.354 ± 0.092
1.105IlePhe: 1.105 ± 0.065
3.447IleGly: 3.447 ± 0.106
0.804IleHis: 0.804 ± 0.039
1.087IleIle: 1.087 ± 0.068
0.649IleLys: 0.649 ± 0.052
3.132IleLeu: 3.132 ± 0.108
0.44IleMet: 0.44 ± 0.035
0.759IleAsn: 0.759 ± 0.05
2.344IlePro: 2.344 ± 0.078
1.003IleGln: 1.003 ± 0.057
3.025IleArg: 3.025 ± 0.094
1.679IleSer: 1.679 ± 0.081
2.022IleThr: 2.022 ± 0.076
3.295IleVal: 3.295 ± 0.103
0.375IleTrp: 0.375 ± 0.031
0.838IleTyr: 0.838 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
2.255LysAla: 2.255 ± 0.091
0.107LysCys: 0.107 ± 0.02
1.016LysAsp: 1.016 ± 0.066
1.184LysGlu: 1.184 ± 0.067
0.547LysPhe: 0.547 ± 0.04
1.605LysGly: 1.605 ± 0.073
0.474LysHis: 0.474 ± 0.035
0.859LysIle: 0.859 ± 0.058
0.752LysLys: 0.752 ± 0.057
2.158LysLeu: 2.158 ± 0.076
0.443LysMet: 0.443 ± 0.037
0.485LysAsn: 0.485 ± 0.039
1.231LysPro: 1.231 ± 0.061
0.608LysGln: 0.608 ± 0.046
1.629LysArg: 1.629 ± 0.072
0.951LysSer: 0.951 ± 0.047
1.105LysThr: 1.105 ± 0.057
1.694LysVal: 1.694 ± 0.073
0.288LysTrp: 0.288 ± 0.028
0.445LysTyr: 0.445 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
14.381LeuAla: 14.381 ± 0.243
0.739LeuCys: 0.739 ± 0.048
5.599LeuAsp: 5.599 ± 0.126
6.013LeuGlu: 6.013 ± 0.156
3.292LeuPhe: 3.292 ± 0.104
8.766LeuGly: 8.766 ± 0.178
1.954LeuHis: 1.954 ± 0.08
2.959LeuIle: 2.959 ± 0.099
2.051LeuLys: 2.051 ± 0.074
10.088LeuLeu: 10.088 ± 0.219
1.687LeuMet: 1.687 ± 0.072
2.022LeuAsn: 2.022 ± 0.067
6.005LeuPro: 6.005 ± 0.122
2.863LeuGln: 2.863 ± 0.076
9.512LeuArg: 9.512 ± 0.172
5.408LeuSer: 5.408 ± 0.144
4.79LeuThr: 4.79 ± 0.121
7.941LeuVal: 7.941 ± 0.175
1.351LeuTrp: 1.351 ± 0.071
2.066LeuTyr: 2.066 ± 0.07
0.0LeuXaa: 0.0 ± 0.0
Met
2.229MetAla: 2.229 ± 0.072
0.097MetCys: 0.097 ± 0.016
1.014MetAsp: 1.014 ± 0.05
1.207MetGlu: 1.207 ± 0.056
0.432MetPhe: 0.432 ± 0.034
1.7MetGly: 1.7 ± 0.065
0.414MetHis: 0.414 ± 0.031
0.752MetIle: 0.752 ± 0.048
0.681MetLys: 0.681 ± 0.047
2.006MetLeu: 2.006 ± 0.075
0.466MetMet: 0.466 ± 0.038
0.553MetAsn: 0.553 ± 0.037
1.155MetPro: 1.155 ± 0.059
0.568MetGln: 0.568 ± 0.044
1.886MetArg: 1.886 ± 0.061
1.252MetSer: 1.252 ± 0.064
1.207MetThr: 1.207 ± 0.057
1.446MetVal: 1.446 ± 0.057
0.189MetTrp: 0.189 ± 0.019
0.343MetTyr: 0.343 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.527AsnAla: 2.527 ± 0.092
0.202AsnCys: 0.202 ± 0.025
1.131AsnAsp: 1.131 ± 0.054
0.979AsnGlu: 0.979 ± 0.057
0.731AsnPhe: 0.731 ± 0.057
1.975AsnGly: 1.975 ± 0.074
0.45AsnHis: 0.45 ± 0.04
0.649AsnIle: 0.649 ± 0.049
0.333AsnLys: 0.333 ± 0.032
2.119AsnLeu: 2.119 ± 0.088
0.288AsnMet: 0.288 ± 0.026
0.526AsnAsn: 0.526 ± 0.049
1.639AsnPro: 1.639 ± 0.07
0.584AsnGln: 0.584 ± 0.045
1.626AsnArg: 1.626 ± 0.071
0.964AsnSer: 0.964 ± 0.064
1.037AsnThr: 1.037 ± 0.063
1.865AsnVal: 1.865 ± 0.08
0.265AsnTrp: 0.265 ± 0.025
0.487AsnTyr: 0.487 ± 0.045
0.0AsnXaa: 0.0 ± 0.0
Pro
8.648ProAla: 8.648 ± 0.206
0.38ProCys: 0.38 ± 0.038
3.622ProAsp: 3.622 ± 0.11
4.062ProGlu: 4.062 ± 0.121
1.938ProPhe: 1.938 ± 0.077
5.877ProGly: 5.877 ± 0.141
1.296ProHis: 1.296 ± 0.061
1.833ProIle: 1.833 ± 0.069
0.893ProLys: 0.893 ± 0.048
5.264ProLeu: 5.264 ± 0.127
1.095ProMet: 1.095 ± 0.059
1.215ProAsn: 1.215 ± 0.05
4.101ProPro: 4.101 ± 0.161
1.454ProGln: 1.454 ± 0.067
4.701ProArg: 4.701 ± 0.131
2.996ProSer: 2.996 ± 0.089
2.781ProThr: 2.781 ± 0.091
4.934ProVal: 4.934 ± 0.126
0.896ProTrp: 0.896 ± 0.051
1.249ProTyr: 1.249 ± 0.062
0.0ProXaa: 0.0 ± 0.0
Gln
3.703GlnAla: 3.703 ± 0.12
0.157GlnCys: 0.157 ± 0.02
1.134GlnAsp: 1.134 ± 0.048
1.469GlnGlu: 1.469 ± 0.065
0.875GlnPhe: 0.875 ± 0.058
2.258GlnGly: 2.258 ± 0.071
0.553GlnHis: 0.553 ± 0.04
1.142GlnIle: 1.142 ± 0.068
0.71GlnLys: 0.71 ± 0.055
2.828GlnLeu: 2.828 ± 0.092
0.56GlnMet: 0.56 ± 0.031
0.686GlnAsn: 0.686 ± 0.048
1.938GlnPro: 1.938 ± 0.087
1.22GlnGln: 1.22 ± 0.067
2.464GlnArg: 2.464 ± 0.077
1.336GlnSer: 1.336 ± 0.059
1.443GlnThr: 1.443 ± 0.079
2.708GlnVal: 2.708 ± 0.095
0.398GlnTrp: 0.398 ± 0.033
0.584GlnTyr: 0.584 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
11.293ArgAla: 11.293 ± 0.214
0.534ArgCys: 0.534 ± 0.042
4.51ArgAsp: 4.51 ± 0.119
6.115ArgGlu: 6.115 ± 0.167
3.352ArgPhe: 3.352 ± 0.098
7.1ArgGly: 7.1 ± 0.172
1.844ArgHis: 1.844 ± 0.074
3.965ArgIle: 3.965 ± 0.097
1.726ArgLys: 1.726 ± 0.069
9.151ArgLeu: 9.151 ± 0.188
2.182ArgMet: 2.182 ± 0.073
1.605ArgAsn: 1.605 ± 0.068
4.856ArgPro: 4.856 ± 0.136
2.551ArgGln: 2.551 ± 0.088
8.519ArgArg: 8.519 ± 0.182
3.866ArgSer: 3.866 ± 0.099
4.518ArgThr: 4.518 ± 0.102
7.097ArgVal: 7.097 ± 0.172
1.637ArgTrp: 1.637 ± 0.075
2.14ArgTyr: 2.14 ± 0.08
0.0ArgXaa: 0.0 ± 0.0
Ser
6.361SerAla: 6.361 ± 0.164
0.422SerCys: 0.422 ± 0.041
2.425SerAsp: 2.425 ± 0.081
2.31SerGlu: 2.31 ± 0.074
1.941SerPhe: 1.941 ± 0.075
5.094SerGly: 5.094 ± 0.122
0.943SerHis: 0.943 ± 0.043
1.983SerIle: 1.983 ± 0.075
0.867SerLys: 0.867 ± 0.054
4.945SerLeu: 4.945 ± 0.12
1.032SerMet: 1.032 ± 0.056
1.066SerAsn: 1.066 ± 0.067
3.279SerPro: 3.279 ± 0.09
1.189SerGln: 1.189 ± 0.058
3.811SerArg: 3.811 ± 0.097
2.669SerSer: 2.669 ± 0.102
2.821SerThr: 2.821 ± 0.078
3.981SerVal: 3.981 ± 0.11
0.77SerTrp: 0.77 ± 0.05
1.1SerTyr: 1.1 ± 0.055
0.0SerXaa: 0.0 ± 0.0
Thr
6.744ThrAla: 6.744 ± 0.159
0.571ThrCys: 0.571 ± 0.049
2.627ThrAsp: 2.627 ± 0.079
2.488ThrGlu: 2.488 ± 0.083
1.883ThrPhe: 1.883 ± 0.086
5.453ThrGly: 5.453 ± 0.149
1.024ThrHis: 1.024 ± 0.056
1.943ThrIle: 1.943 ± 0.076
0.762ThrLys: 0.762 ± 0.047
5.814ThrLeu: 5.814 ± 0.136
0.838ThrMet: 0.838 ± 0.054
1.045ThrAsn: 1.045 ± 0.062
3.604ThrPro: 3.604 ± 0.105
1.438ThrGln: 1.438 ± 0.072
4.033ThrArg: 4.033 ± 0.106
2.525ThrSer: 2.525 ± 0.105
2.776ThrThr: 2.776 ± 0.112
5.044ThrVal: 5.044 ± 0.153
0.825ThrTrp: 0.825 ± 0.06
1.163ThrTyr: 1.163 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
10.746ValAla: 10.746 ± 0.205
0.623ValCys: 0.623 ± 0.04
5.199ValAsp: 5.199 ± 0.127
5.728ValGlu: 5.728 ± 0.144
2.753ValPhe: 2.753 ± 0.079
6.613ValGly: 6.613 ± 0.144
1.833ValHis: 1.833 ± 0.066
3.072ValIle: 3.072 ± 0.102
1.763ValLys: 1.763 ± 0.067
8.415ValLeu: 8.415 ± 0.15
1.532ValMet: 1.532 ± 0.068
1.938ValAsn: 1.938 ± 0.073
4.795ValPro: 4.795 ± 0.122
2.404ValGln: 2.404 ± 0.082
7.385ValArg: 7.385 ± 0.16
4.264ValSer: 4.264 ± 0.117
4.832ValThr: 4.832 ± 0.149
7.333ValVal: 7.333 ± 0.156
1.042ValTrp: 1.042 ± 0.054
1.88ValTyr: 1.88 ± 0.087
0.0ValXaa: 0.0 ± 0.0
Trp
1.519TrpAla: 1.519 ± 0.074
0.149TrpCys: 0.149 ± 0.025
0.744TrpAsp: 0.744 ± 0.049
0.909TrpGlu: 0.909 ± 0.049
0.597TrpPhe: 0.597 ± 0.045
1.207TrpGly: 1.207 ± 0.061
0.343TrpHis: 0.343 ± 0.031
0.678TrpIle: 0.678 ± 0.037
0.445TrpLys: 0.445 ± 0.034
1.637TrpLeu: 1.637 ± 0.07
0.461TrpMet: 0.461 ± 0.031
0.419TrpAsn: 0.419 ± 0.035
0.574TrpPro: 0.574 ± 0.044
0.411TrpGln: 0.411 ± 0.036
1.406TrpArg: 1.406 ± 0.062
0.883TrpSer: 0.883 ± 0.055
1.071TrpThr: 1.071 ± 0.069
1.034TrpVal: 1.034 ± 0.056
0.278TrpTrp: 0.278 ± 0.03
0.385TrpTyr: 0.385 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.705TyrAla: 2.705 ± 0.098
0.233TyrCys: 0.233 ± 0.029
1.438TyrAsp: 1.438 ± 0.071
1.147TyrGlu: 1.147 ± 0.056
0.809TyrPhe: 0.809 ± 0.046
2.229TyrGly: 2.229 ± 0.084
0.469TyrHis: 0.469 ± 0.034
0.56TyrIle: 0.56 ± 0.046
0.377TyrLys: 0.377 ± 0.031
2.313TyrLeu: 2.313 ± 0.073
0.317TyrMet: 0.317 ± 0.033
0.558TyrAsn: 0.558 ± 0.044
1.346TyrPro: 1.346 ± 0.064
0.689TyrGln: 0.689 ± 0.045
2.281TyrArg: 2.281 ± 0.08
1.084TyrSer: 1.084 ± 0.057
1.181TyrThr: 1.181 ± 0.063
2.011TyrVal: 2.011 ± 0.081
0.335TyrTrp: 0.335 ± 0.031
0.626TyrTyr: 0.626 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1208 proteins (381834 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski