Amino acid dipepetide frequency for Flavobacterium sp. GSN2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.781AlaAla: 4.781 ± 0.072	0.529AlaCys: 0.529 ± 0.019	3.4AlaAsp: 3.4 ± 0.044	3.939AlaGlu: 3.939 ± 0.053	3.431AlaPhe: 3.431 ± 0.045	4.417AlaGly: 4.417 ± 0.07	1.067AlaHis: 1.067 ± 0.027	5.752AlaIle: 5.752 ± 0.059	4.962AlaLys: 4.962 ± 0.068	6.305AlaLeu: 6.305 ± 0.073	1.613AlaMet: 1.613 ± 0.036	3.706AlaAsn: 3.706 ± 0.057	2.006AlaPro: 2.006 ± 0.044	2.479AlaGln: 2.479 ± 0.038	1.942AlaArg: 1.942 ± 0.037	4.301AlaSer: 4.301 ± 0.054	4.32AlaThr: 4.32 ± 0.068	4.473AlaVal: 4.473 ± 0.056	0.604AlaTrp: 0.604 ± 0.018	2.341AlaTyr: 2.341 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.492CysAla: 0.492 ± 0.017	0.112CysCys: 0.112 ± 0.009	0.441CysAsp: 0.441 ± 0.017	0.436CysGlu: 0.436 ± 0.016	0.405CysPhe: 0.405 ± 0.015	0.627CysGly: 0.627 ± 0.023	0.193CysHis: 0.193 ± 0.011	0.612CysIle: 0.612 ± 0.018	0.468CysLys: 0.468 ± 0.015	0.649CysLeu: 0.649 ± 0.021	0.146CysMet: 0.146 ± 0.009	0.437CysAsn: 0.437 ± 0.021	0.332CysPro: 0.332 ± 0.017	0.235CysGln: 0.235 ± 0.011	0.203CysArg: 0.203 ± 0.01	0.586CysSer: 0.586 ± 0.019	0.475CysThr: 0.475 ± 0.018	0.438CysVal: 0.438 ± 0.016	0.067CysTrp: 0.067 ± 0.006	0.309CysTyr: 0.309 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	3.736AspAla: 3.736 ± 0.052	0.428AspCys: 0.428 ± 0.017	2.476AspAsp: 2.476 ± 0.039	3.361AspGlu: 3.361 ± 0.051	3.639AspPhe: 3.639 ± 0.047	3.479AspGly: 3.479 ± 0.051	0.807AspHis: 0.807 ± 0.022	4.183AspIle: 4.183 ± 0.058	4.184AspLys: 4.184 ± 0.055	5.069AspLeu: 5.069 ± 0.053	1.084AspMet: 1.084 ± 0.023	2.998AspAsn: 2.998 ± 0.046	1.591AspPro: 1.591 ± 0.032	1.467AspGln: 1.467 ± 0.03	1.693AspArg: 1.693 ± 0.032	2.978AspSer: 2.978 ± 0.037	2.763AspThr: 2.763 ± 0.04	3.458AspVal: 3.458 ± 0.044	0.684AspTrp: 0.684 ± 0.018	2.689AspTyr: 2.689 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	4.123GluAla: 4.123 ± 0.066	0.341GluCys: 0.341 ± 0.016	3.046GluAsp: 3.046 ± 0.042	4.236GluGlu: 4.236 ± 0.06	2.835GluPhe: 2.835 ± 0.041	3.307GluGly: 3.307 ± 0.044	1.04GluHis: 1.04 ± 0.027	5.776GluIle: 5.776 ± 0.068	6.068GluLys: 6.068 ± 0.07	5.808GluLeu: 5.808 ± 0.063	1.7GluMet: 1.7 ± 0.028	4.74GluAsn: 4.74 ± 0.055	1.417GluPro: 1.417 ± 0.029	2.102GluGln: 2.102 ± 0.034	2.27GluArg: 2.27 ± 0.039	3.214GluSer: 3.214 ± 0.039	3.657GluThr: 3.657 ± 0.041	3.937GluVal: 3.937 ± 0.049	0.556GluTrp: 0.556 ± 0.017	2.274GluTyr: 2.274 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.255PheAla: 3.255 ± 0.046	0.48PheCys: 0.48 ± 0.018	3.36PheAsp: 3.36 ± 0.046	3.516PheGlu: 3.516 ± 0.054	2.788PhePhe: 2.788 ± 0.053	3.712PheGly: 3.712 ± 0.046	0.908PheHis: 0.908 ± 0.021	4.08PheIle: 4.08 ± 0.058	3.622PheLys: 3.622 ± 0.046	4.839PheLeu: 4.839 ± 0.056	1.205PheMet: 1.205 ± 0.027	3.197PheAsn: 3.197 ± 0.056	1.728PhePro: 1.728 ± 0.032	1.71PheGln: 1.71 ± 0.03	1.646PheArg: 1.646 ± 0.031	4.169PheSer: 4.169 ± 0.053	3.192PheThr: 3.192 ± 0.046	3.152PheVal: 3.152 ± 0.044	0.54PheTrp: 0.54 ± 0.021	2.191PheTyr: 2.191 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	4.179GlyAla: 4.179 ± 0.063	0.641GlyCys: 0.641 ± 0.025	3.089GlyAsp: 3.089 ± 0.048	3.225GlyGlu: 3.225 ± 0.047	3.703GlyPhe: 3.703 ± 0.051	4.502GlyGly: 4.502 ± 0.074	1.126GlyHis: 1.126 ± 0.023	5.681GlyIle: 5.681 ± 0.063	5.072GlyLys: 5.072 ± 0.051	5.638GlyLeu: 5.638 ± 0.058	1.673GlyMet: 1.673 ± 0.032	3.736GlyAsn: 3.736 ± 0.06	1.324GlyPro: 1.324 ± 0.027	1.945GlyGln: 1.945 ± 0.036	1.969GlyArg: 1.969 ± 0.033	4.136GlySer: 4.136 ± 0.063	4.523GlyThr: 4.523 ± 0.082	4.197GlyVal: 4.197 ± 0.051	0.67GlyTrp: 0.67 ± 0.02	2.745GlyTyr: 2.745 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	0.949HisAla: 0.949 ± 0.027	0.183HisCys: 0.183 ± 0.009	0.848HisAsp: 0.848 ± 0.021	0.96HisGlu: 0.96 ± 0.024	1.178HisPhe: 1.178 ± 0.024	1.008HisGly: 1.008 ± 0.023	0.477HisHis: 0.477 ± 0.018	1.361HisIle: 1.361 ± 0.025	1.145HisLys: 1.145 ± 0.024	1.715HisLeu: 1.715 ± 0.031	0.285HisMet: 0.285 ± 0.013	0.961HisAsn: 0.961 ± 0.022	0.83HisPro: 0.83 ± 0.024	0.735HisGln: 0.735 ± 0.019	0.614HisArg: 0.614 ± 0.02	1.109HisSer: 1.109 ± 0.024	0.898HisThr: 0.898 ± 0.02	0.884HisVal: 0.884 ± 0.022	0.197HisTrp: 0.197 ± 0.013	0.796HisTyr: 0.796 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.052IleAla: 6.052 ± 0.057	0.677IleCys: 0.677 ± 0.023	4.828IleAsp: 4.828 ± 0.05	5.356IleGlu: 5.356 ± 0.062	3.776IlePhe: 3.776 ± 0.064	5.319IleGly: 5.319 ± 0.062	1.441IleHis: 1.441 ± 0.03	6.685IleIle: 6.685 ± 0.077	6.075IleLys: 6.075 ± 0.064	7.343IleLeu: 7.343 ± 0.073	1.561IleMet: 1.561 ± 0.028	4.915IleAsn: 4.915 ± 0.064	3.476IlePro: 3.476 ± 0.048	2.952IleGln: 2.952 ± 0.042	2.628IleArg: 2.628 ± 0.042	5.685IleSer: 5.685 ± 0.061	5.15IleThr: 5.15 ± 0.059	5.195IleVal: 5.195 ± 0.057	0.721IleTrp: 0.721 ± 0.021	2.799IleTyr: 2.799 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.965LysAla: 4.965 ± 0.06	0.358LysCys: 0.358 ± 0.014	4.104LysAsp: 4.104 ± 0.047	5.841LysGlu: 5.841 ± 0.075	3.087LysPhe: 3.087 ± 0.04	4.552LysGly: 4.552 ± 0.051	1.186LysHis: 1.186 ± 0.023	7.117LysIle: 7.117 ± 0.061	7.107LysLys: 7.107 ± 0.08	6.395LysLeu: 6.395 ± 0.061	2.378LysMet: 2.378 ± 0.035	5.52LysAsn: 5.52 ± 0.066	2.487LysPro: 2.487 ± 0.039	2.641LysGln: 2.641 ± 0.043	2.632LysArg: 2.632 ± 0.043	4.667LysSer: 4.667 ± 0.053	4.959LysThr: 4.959 ± 0.055	4.687LysVal: 4.687 ± 0.054	0.764LysTrp: 0.764 ± 0.02	3.103LysTyr: 3.103 ± 0.044	0.0LysXaa: 0.0 ± 0.0
Leu	5.997LeuAla: 5.997 ± 0.064	0.656LeuCys: 0.656 ± 0.019	4.883LeuAsp: 4.883 ± 0.055	5.735LeuGlu: 5.735 ± 0.061	5.093LeuPhe: 5.093 ± 0.066	5.677LeuGly: 5.677 ± 0.054	1.574LeuHis: 1.574 ± 0.029	7.068LeuIle: 7.068 ± 0.072	7.457LeuLys: 7.457 ± 0.078	8.719LeuLeu: 8.719 ± 0.094	2.185LeuMet: 2.185 ± 0.044	5.631LeuAsn: 5.631 ± 0.062	3.457LeuPro: 3.457 ± 0.054	3.302LeuGln: 3.302 ± 0.048	2.916LeuArg: 2.916 ± 0.044	6.595LeuSer: 6.595 ± 0.06	5.133LeuThr: 5.133 ± 0.058	5.353LeuVal: 5.353 ± 0.064	0.725LeuTrp: 0.725 ± 0.017	3.125LeuTyr: 3.125 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	1.64MetAla: 1.64 ± 0.032	0.14MetCys: 0.14 ± 0.009	1.225MetAsp: 1.225 ± 0.027	1.471MetGlu: 1.471 ± 0.03	0.883MetPhe: 0.883 ± 0.023	1.421MetGly: 1.421 ± 0.027	0.424MetHis: 0.424 ± 0.015	1.716MetIle: 1.716 ± 0.032	2.394MetLys: 2.394 ± 0.04	1.927MetLeu: 1.927 ± 0.036	0.667MetMet: 0.667 ± 0.023	1.503MetAsn: 1.503 ± 0.028	0.85MetPro: 0.85 ± 0.022	0.904MetGln: 0.904 ± 0.022	0.872MetArg: 0.872 ± 0.027	1.468MetSer: 1.468 ± 0.027	1.278MetThr: 1.278 ± 0.027	1.42MetVal: 1.42 ± 0.029	0.168MetTrp: 0.168 ± 0.01	0.733MetTyr: 0.733 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	4.059AsnAla: 4.059 ± 0.048	0.499AsnCys: 0.499 ± 0.023	3.171AsnAsp: 3.171 ± 0.042	3.715AsnGlu: 3.715 ± 0.05	3.178AsnPhe: 3.178 ± 0.05	4.166AsnGly: 4.166 ± 0.064	1.122AsnHis: 1.122 ± 0.023	4.793AsnIle: 4.793 ± 0.064	4.518AsnLys: 4.518 ± 0.047	5.554AsnLeu: 5.554 ± 0.064	1.289AsnMet: 1.289 ± 0.028	3.892AsnAsn: 3.892 ± 0.059	3.0AsnPro: 3.0 ± 0.046	2.447AsnGln: 2.447 ± 0.045	2.056AsnArg: 2.056 ± 0.035	4.104AsnSer: 4.104 ± 0.057	3.793AsnThr: 3.793 ± 0.058	3.608AsnVal: 3.608 ± 0.05	0.771AsnTrp: 0.771 ± 0.019	2.878AsnTyr: 2.878 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.189ProAla: 2.189 ± 0.045	0.218ProCys: 0.218 ± 0.01	1.865ProAsp: 1.865 ± 0.029	2.711ProGlu: 2.711 ± 0.038	2.033ProPhe: 2.033 ± 0.033	1.847ProGly: 1.847 ± 0.035	0.538ProHis: 0.538 ± 0.016	2.774ProIle: 2.774 ± 0.037	2.634ProLys: 2.634 ± 0.045	2.879ProLeu: 2.879 ± 0.041	0.762ProMet: 0.762 ± 0.02	2.312ProAsn: 2.312 ± 0.039	0.765ProPro: 0.765 ± 0.024	1.079ProGln: 1.079 ± 0.02	0.896ProArg: 0.896 ± 0.026	2.213ProSer: 2.213 ± 0.037	2.179ProThr: 2.179 ± 0.046	2.435ProVal: 2.435 ± 0.039	0.304ProTrp: 0.304 ± 0.012	1.371ProTyr: 1.371 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	1.98GlnAla: 1.98 ± 0.035	0.205GlnCys: 0.205 ± 0.011	1.556GlnAsp: 1.556 ± 0.027	2.262GlnGlu: 2.262 ± 0.038	1.842GlnPhe: 1.842 ± 0.036	1.742GlnGly: 1.742 ± 0.031	0.584GlnHis: 0.584 ± 0.016	2.851GlnIle: 2.851 ± 0.042	3.161GlnLys: 3.161 ± 0.047	3.447GlnLeu: 3.447 ± 0.058	0.88GlnMet: 0.88 ± 0.021	2.544GlnAsn: 2.544 ± 0.04	1.039GlnPro: 1.039 ± 0.028	1.422GlnGln: 1.422 ± 0.045	1.106GlnArg: 1.106 ± 0.024	2.107GlnSer: 2.107 ± 0.034	2.061GlnThr: 2.061 ± 0.038	1.914GlnVal: 1.914 ± 0.036	0.363GlnTrp: 0.363 ± 0.014	1.371GlnTyr: 1.371 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	1.951ArgAla: 1.951 ± 0.031	0.205ArgCys: 0.205 ± 0.013	1.666ArgAsp: 1.666 ± 0.034	2.019ArgGlu: 2.019 ± 0.035	1.821ArgPhe: 1.821 ± 0.034	1.811ArgGly: 1.811 ± 0.031	0.549ArgHis: 0.549 ± 0.017	2.87ArgIle: 2.87 ± 0.039	2.715ArgLys: 2.715 ± 0.045	2.954ArgLeu: 2.954 ± 0.048	0.927ArgMet: 0.927 ± 0.02	2.082ArgAsn: 2.082 ± 0.034	1.025ArgPro: 1.025 ± 0.025	1.007ArgGln: 1.007 ± 0.023	1.127ArgArg: 1.127 ± 0.028	1.771ArgSer: 1.771 ± 0.037	1.796ArgThr: 1.796 ± 0.032	1.97ArgVal: 1.97 ± 0.039	0.334ArgTrp: 0.334 ± 0.015	1.38ArgTyr: 1.38 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	4.08SerAla: 4.08 ± 0.056	0.645SerCys: 0.645 ± 0.019	3.481SerAsp: 3.481 ± 0.049	3.832SerGlu: 3.832 ± 0.046	3.936SerPhe: 3.936 ± 0.053	4.879SerGly: 4.879 ± 0.072	1.157SerHis: 1.157 ± 0.026	5.169SerIle: 5.169 ± 0.06	4.881SerLys: 4.881 ± 0.054	5.842SerLeu: 5.842 ± 0.068	1.292SerMet: 1.292 ± 0.027	3.853SerAsn: 3.853 ± 0.049	2.174SerPro: 2.174 ± 0.031	2.245SerGln: 2.245 ± 0.034	2.094SerArg: 2.094 ± 0.034	4.332SerSer: 4.332 ± 0.062	3.71SerThr: 3.71 ± 0.065	4.117SerVal: 4.117 ± 0.048	0.691SerTrp: 0.691 ± 0.02	2.734SerTyr: 2.734 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.49ThrAla: 4.49 ± 0.081	0.368ThrCys: 0.368 ± 0.015	3.231ThrAsp: 3.231 ± 0.047	3.448ThrGlu: 3.448 ± 0.042	3.24ThrPhe: 3.24 ± 0.046	4.28ThrGly: 4.28 ± 0.073	0.989ThrHis: 0.989 ± 0.025	5.438ThrIle: 5.438 ± 0.061	4.08ThrLys: 4.08 ± 0.047	5.449ThrLeu: 5.449 ± 0.058	1.07ThrMet: 1.07 ± 0.024	3.587ThrAsn: 3.587 ± 0.062	2.594ThrPro: 2.594 ± 0.042	1.983ThrGln: 1.983 ± 0.033	1.579ThrArg: 1.579 ± 0.031	3.892ThrSer: 3.892 ± 0.057	4.15ThrThr: 4.15 ± 0.082	4.24ThrVal: 4.24 ± 0.058	0.531ThrTrp: 0.531 ± 0.02	2.287ThrTyr: 2.287 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	4.453ValAla: 4.453 ± 0.052	0.526ValCys: 0.526 ± 0.017	3.326ValAsp: 3.326 ± 0.043	3.547ValGlu: 3.547 ± 0.044	3.412ValPhe: 3.412 ± 0.046	3.82ValGly: 3.82 ± 0.049	0.979ValHis: 0.979 ± 0.026	5.165ValIle: 5.165 ± 0.056	4.328ValLys: 4.328 ± 0.051	6.159ValLeu: 6.159 ± 0.063	1.367ValMet: 1.367 ± 0.027	3.69ValAsn: 3.69 ± 0.052	2.217ValPro: 2.217 ± 0.038	1.905ValGln: 1.905 ± 0.033	1.939ValArg: 1.939 ± 0.031	4.456ValSer: 4.456 ± 0.049	3.975ValThr: 3.975 ± 0.062	4.184ValVal: 4.184 ± 0.061	0.579ValTrp: 0.579 ± 0.019	2.362ValTyr: 2.362 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.616TrpAla: 0.616 ± 0.017	0.097TrpCys: 0.097 ± 0.007	0.528TrpAsp: 0.528 ± 0.019	0.622TrpGlu: 0.622 ± 0.02	0.53TrpPhe: 0.53 ± 0.019	0.614TrpGly: 0.614 ± 0.019	0.217TrpHis: 0.217 ± 0.01	0.755TrpIle: 0.755 ± 0.02	0.798TrpLys: 0.798 ± 0.021	0.872TrpLeu: 0.872 ± 0.024	0.272TrpMet: 0.272 ± 0.011	0.723TrpAsn: 0.723 ± 0.021	0.197TrpPro: 0.197 ± 0.011	0.389TrpGln: 0.389 ± 0.015	0.355TrpArg: 0.355 ± 0.015	0.618TrpSer: 0.618 ± 0.02	0.551TrpThr: 0.551 ± 0.018	0.569TrpVal: 0.569 ± 0.018	0.12TrpTrp: 0.12 ± 0.008	0.385TrpTyr: 0.385 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.382TyrAla: 2.382 ± 0.039	0.354TyrCys: 0.354 ± 0.013	2.243TyrAsp: 2.243 ± 0.033	2.216TyrGlu: 2.216 ± 0.037	2.475TyrPhe: 2.475 ± 0.04	2.596TyrGly: 2.596 ± 0.043	0.754TyrHis: 0.754 ± 0.016	2.789TyrIle: 2.789 ± 0.044	2.881TyrLys: 2.881 ± 0.039	3.717TyrLeu: 3.717 ± 0.054	0.747TyrMet: 0.747 ± 0.022	2.587TyrAsn: 2.587 ± 0.045	1.495TyrPro: 1.495 ± 0.029	1.489TyrGln: 1.489 ± 0.027	1.444TyrArg: 1.444 ± 0.029	2.743TyrSer: 2.743 ± 0.043	2.361TyrThr: 2.361 ± 0.04	2.159TyrVal: 2.159 ± 0.037	0.44TyrTrp: 0.44 ± 0.016	1.798TyrTyr: 1.798 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5511 proteins (1879230 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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