Desulfarculus baarsii (strain ATCC 33931 / DSM 2075 / LMG 7858 / VKM B-1802 / 2st14)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfarculales; Desulfarculaceae; Desulfarculus; Desulfarculus baarsii

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All


Virtual 2D-PAGE plot for 3268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1QHJ2|E1QHJ2_DESB2 Binding-protein-dependent transport systems inner membrane component OS=Desulfarculus baarsii (strain ATCC 33931 / DSM 2075 / LMG 7858 / VKM B-1802 / 2st14) OX=644282 GN=Deba_1667 PE=3 SV=1
MM1 pKa = 7.1QATSFFNGVSGLKK14 pKa = 10.4SFSQGLNIVADD25 pKa = 3.71NLANSNTYY33 pKa = 10.0GYY35 pKa = 10.09KK36 pKa = 9.78SSRR39 pKa = 11.84AEE41 pKa = 3.95FADD44 pKa = 3.21VLYY47 pKa = 10.92RR48 pKa = 11.84EE49 pKa = 4.49LSYY52 pKa = 10.79TGSDD56 pKa = 3.14LTTEE60 pKa = 4.34IDD62 pKa = 3.68QVGQGATVWSHH73 pKa = 5.67QLMNQGAIQEE83 pKa = 4.4TGRR86 pKa = 11.84TLDD89 pKa = 4.28LAIDD93 pKa = 3.84GNGFFTVKK101 pKa = 10.64NLDD104 pKa = 3.71TEE106 pKa = 4.16EE107 pKa = 4.65LYY109 pKa = 9.3YY110 pKa = 10.78TRR112 pKa = 11.84AGQFGVDD119 pKa = 3.25GVVGQEE125 pKa = 3.74GFIINDD131 pKa = 2.86QGYY134 pKa = 8.69RR135 pKa = 11.84LQGFAIGDD143 pKa = 3.77DD144 pKa = 3.95GEE146 pKa = 4.85PIVGNLIDD154 pKa = 4.06LQIPVEE160 pKa = 4.11NLPGEE165 pKa = 4.18ATTIVGLGVNLNPADD180 pKa = 3.63TRR182 pKa = 11.84VNQVATDD189 pKa = 3.78IDD191 pKa = 4.19PEE193 pKa = 4.23VSGTYY198 pKa = 9.88NYY200 pKa = 10.81SSSTTVYY207 pKa = 10.37DD208 pKa = 4.03ANGDD212 pKa = 3.56THH214 pKa = 6.93QISVYY219 pKa = 7.03YY220 pKa = 10.23QRR222 pKa = 11.84VDD224 pKa = 3.81DD225 pKa = 4.2YY226 pKa = 11.73AGTVPEE232 pKa = 5.02GGQTVWKK239 pKa = 10.09ASTFEE244 pKa = 4.26TQDD247 pKa = 3.29GEE249 pKa = 4.69QVANPADD256 pKa = 3.9PANTFYY262 pKa = 11.5LHH264 pKa = 5.95FTDD267 pKa = 3.83TGALAGVTDD276 pKa = 3.85STGATVSADD285 pKa = 3.97SIGLTMDD292 pKa = 4.01FGEE295 pKa = 5.26AGQQAITLDD304 pKa = 3.98FAPAAGQATTQVAEE318 pKa = 5.08GYY320 pKa = 7.76STSTNTQDD328 pKa = 3.38GFAEE332 pKa = 4.84GGLEE336 pKa = 4.0SVAVSEE342 pKa = 4.96DD343 pKa = 3.73GFVTAYY349 pKa = 9.92YY350 pKa = 11.0SNGEE354 pKa = 4.12MVDD357 pKa = 3.48VGVVALTTFASPGNLRR373 pKa = 11.84RR374 pKa = 11.84EE375 pKa = 4.16GDD377 pKa = 3.72NLWAYY382 pKa = 10.44DD383 pKa = 3.8ADD385 pKa = 4.43AGEE388 pKa = 4.26IWVGQPTDD396 pKa = 3.36EE397 pKa = 4.28EE398 pKa = 4.51FAMGAIEE405 pKa = 4.41DD406 pKa = 3.95QSLEE410 pKa = 4.08TSTVDD415 pKa = 3.36TATEE419 pKa = 3.92MMNMIIYY426 pKa = 9.75QRR428 pKa = 11.84AFQASSKK435 pKa = 9.68TVTTSDD441 pKa = 3.47EE442 pKa = 4.31MIKK445 pKa = 9.14TAINMKK451 pKa = 8.72TT452 pKa = 3.05

Molecular weight: 48.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1QGF0|E1QGF0_DESB2 ATP synthase subunit alpha OS=Desulfarculus baarsii (strain ATCC 33931 / DSM 2075 / LMG 7858 / VKM B-1802 / 2st14) OX=644282 GN=atpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.55RR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84SRR23 pKa = 11.84SKK25 pKa = 10.78AGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25GRR39 pKa = 11.84ARR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3268

0

3268

1096967

30

2142

335.7

36.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.682 ± 0.07

1.27 ± 0.017

5.504 ± 0.031

5.793 ± 0.035

3.402 ± 0.023

8.842 ± 0.043

2.001 ± 0.018

4.452 ± 0.031

3.666 ± 0.036

11.245 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.667 ± 0.019

2.538 ± 0.026

5.178 ± 0.032

4.094 ± 0.027

7.094 ± 0.036

4.588 ± 0.029

4.124 ± 0.026

7.261 ± 0.037

1.318 ± 0.019

2.281 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski