Sinorhizobium phage phi3LM21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291AUT5|A0A291AUT5_9CAUD Uncharacterized protein OS=Sinorhizobium phage phi3LM21 OX=2029634 GN=phi3LM21_p25 PE=4 SV=1
MM1 pKa = 7.71AIEE4 pKa = 4.05TDD6 pKa = 3.23ADD8 pKa = 3.82RR9 pKa = 11.84LLFLDD14 pKa = 4.03EE15 pKa = 5.45DD16 pKa = 4.01EE17 pKa = 5.3FGASIVWTSASGTKK31 pKa = 9.9PPSSCIFDD39 pKa = 3.7DD40 pKa = 3.86TFVALGAGEE49 pKa = 5.22LDD51 pKa = 3.57LTQEE55 pKa = 4.33GGRR58 pKa = 11.84VSVTMRR64 pKa = 11.84TRR66 pKa = 11.84DD67 pKa = 3.44VPGDD71 pKa = 3.68AEE73 pKa = 4.43HH74 pKa = 7.19EE75 pKa = 4.53DD76 pKa = 3.96GVVVSSEE83 pKa = 3.79ILGSKK88 pKa = 8.44SFRR91 pKa = 11.84VVEE94 pKa = 4.46FQPDD98 pKa = 3.79GTGMTIVRR106 pKa = 11.84LMEE109 pKa = 3.95VV110 pKa = 3.1

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291AUR3|A0A291AUR3_9CAUD Uncharacterized protein OS=Sinorhizobium phage phi3LM21 OX=2029634 GN=phi3LM21_p11 PE=4 SV=1
MM1 pKa = 7.33SASSLAVPEE10 pKa = 4.17RR11 pKa = 11.84TKK13 pKa = 10.97SFPHH17 pKa = 6.43NLTPADD23 pKa = 3.28VDD25 pKa = 3.92KK26 pKa = 10.85IRR28 pKa = 11.84RR29 pKa = 11.84MVLAGNPTAEE39 pKa = 3.82IAAAFGTTEE48 pKa = 3.35RR49 pKa = 11.84RR50 pKa = 11.84AEE52 pKa = 3.85VFLAVRR58 pKa = 11.84RR59 pKa = 11.84PRR61 pKa = 11.84WLRR64 pKa = 11.84YY65 pKa = 8.67RR66 pKa = 11.84DD67 pKa = 3.32EE68 pKa = 4.13GRR70 pKa = 11.84IEE72 pKa = 4.16FMPEE76 pKa = 3.04KK77 pKa = 9.67TVFYY81 pKa = 10.1RR82 pKa = 11.84WRR84 pKa = 11.84SRR86 pKa = 11.84DD87 pKa = 3.25DD88 pKa = 3.09GGRR91 pKa = 11.84DD92 pKa = 3.44LRR94 pKa = 11.84PFSVAAISMHH104 pKa = 6.06RR105 pKa = 11.84AALEE109 pKa = 4.08TQAA112 pKa = 5.18

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13095

36

894

221.9

24.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.31 ± 0.395

0.939 ± 0.143

6.201 ± 0.172

6.476 ± 0.361

3.719 ± 0.187

8.37 ± 0.397

1.642 ± 0.141

4.796 ± 0.146

4.2 ± 0.238

8.125 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.146

2.818 ± 0.178

4.987 ± 0.267

3.811 ± 0.227

7.468 ± 0.409

5.819 ± 0.317

5.819 ± 0.355

7.079 ± 0.298

1.879 ± 0.162

2.283 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski