Amino acid dipepetide frequency for Sinorhizobium phage phi3LM21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.656AlaAla: 15.656 ± 1.715
0.535AlaCys: 0.535 ± 0.242
7.866AlaAsp: 7.866 ± 0.775
8.554AlaGlu: 8.554 ± 1.089
3.666AlaPhe: 3.666 ± 0.622
8.783AlaGly: 8.783 ± 0.975
1.68AlaHis: 1.68 ± 0.341
4.811AlaIle: 4.811 ± 0.626
5.499AlaLys: 5.499 ± 0.849
9.699AlaLeu: 9.699 ± 0.89
3.055AlaMet: 3.055 ± 0.571
3.513AlaAsn: 3.513 ± 0.542
4.048AlaPro: 4.048 ± 0.517
4.048AlaGln: 4.048 ± 0.65
7.408AlaArg: 7.408 ± 0.748
6.186AlaSer: 6.186 ± 0.845
6.568AlaThr: 6.568 ± 0.778
7.255AlaVal: 7.255 ± 0.667
2.444AlaTrp: 2.444 ± 0.449
1.833AlaTyr: 1.833 ± 0.441
0.0AlaXaa: 0.0 ± 0.0
Cys
1.069CysAla: 1.069 ± 0.298
0.153CysCys: 0.153 ± 0.093
0.764CysAsp: 0.764 ± 0.291
0.458CysGlu: 0.458 ± 0.201
0.229CysPhe: 0.229 ± 0.153
0.382CysGly: 0.382 ± 0.149
0.153CysHis: 0.153 ± 0.116
0.382CysIle: 0.382 ± 0.144
0.229CysLys: 0.229 ± 0.137
0.84CysLeu: 0.84 ± 0.261
0.153CysMet: 0.153 ± 0.106
0.305CysAsn: 0.305 ± 0.181
0.535CysPro: 0.535 ± 0.21
0.458CysGln: 0.458 ± 0.161
0.535CysArg: 0.535 ± 0.204
0.764CysSer: 0.764 ± 0.227
0.687CysThr: 0.687 ± 0.255
0.611CysVal: 0.611 ± 0.195
0.382CysTrp: 0.382 ± 0.174
0.305CysTyr: 0.305 ± 0.149
0.0CysXaa: 0.0 ± 0.0
Asp
6.873AspAla: 6.873 ± 0.865
0.611AspCys: 0.611 ± 0.214
3.971AspAsp: 3.971 ± 0.625
4.277AspGlu: 4.277 ± 0.522
2.597AspPhe: 2.597 ± 0.343
6.492AspGly: 6.492 ± 0.82
0.764AspHis: 0.764 ± 0.276
3.513AspIle: 3.513 ± 0.475
1.986AspLys: 1.986 ± 0.486
5.575AspLeu: 5.575 ± 0.575
1.604AspMet: 1.604 ± 0.371
1.222AspAsn: 1.222 ± 0.248
2.749AspPro: 2.749 ± 0.501
2.597AspGln: 2.597 ± 0.521
4.811AspArg: 4.811 ± 0.756
1.527AspSer: 1.527 ± 0.319
3.589AspThr: 3.589 ± 0.453
4.43AspVal: 4.43 ± 0.548
1.375AspTrp: 1.375 ± 0.288
1.451AspTyr: 1.451 ± 0.306
0.0AspXaa: 0.0 ± 0.0
Glu
7.484GluAla: 7.484 ± 0.72
0.382GluCys: 0.382 ± 0.176
3.284GluAsp: 3.284 ± 0.574
4.277GluGlu: 4.277 ± 0.688
2.52GluPhe: 2.52 ± 0.521
4.811GluGly: 4.811 ± 0.582
1.222GluHis: 1.222 ± 0.316
2.902GluIle: 2.902 ± 0.544
2.673GluLys: 2.673 ± 0.491
5.957GluLeu: 5.957 ± 0.747
2.597GluMet: 2.597 ± 0.474
1.451GluAsn: 1.451 ± 0.304
2.52GluPro: 2.52 ± 0.536
2.978GluGln: 2.978 ± 0.457
6.11GluArg: 6.11 ± 0.646
3.36GluSer: 3.36 ± 0.5
3.819GluThr: 3.819 ± 0.621
3.819GluVal: 3.819 ± 0.563
1.069GluTrp: 1.069 ± 0.302
1.527GluTyr: 1.527 ± 0.29
0.0GluXaa: 0.0 ± 0.0
Phe
3.971PheAla: 3.971 ± 0.437
0.687PheCys: 0.687 ± 0.2
3.742PheAsp: 3.742 ± 0.474
2.673PheGlu: 2.673 ± 0.471
1.986PhePhe: 1.986 ± 0.385
3.131PheGly: 3.131 ± 0.465
0.535PheHis: 0.535 ± 0.184
1.222PheIle: 1.222 ± 0.386
1.527PheLys: 1.527 ± 0.394
2.444PheLeu: 2.444 ± 0.385
0.764PheMet: 0.764 ± 0.199
0.993PheAsn: 0.993 ± 0.302
2.52PhePro: 2.52 ± 0.374
2.367PheGln: 2.367 ± 0.456
1.757PheArg: 1.757 ± 0.4
2.062PheSer: 2.062 ± 0.559
1.298PheThr: 1.298 ± 0.281
2.444PheVal: 2.444 ± 0.45
0.382PheTrp: 0.382 ± 0.167
0.687PheTyr: 0.687 ± 0.183
0.0PheXaa: 0.0 ± 0.0
Gly
7.713GlyAla: 7.713 ± 0.827
0.764GlyCys: 0.764 ± 0.265
5.346GlyAsp: 5.346 ± 0.66
5.422GlyGlu: 5.422 ± 0.679
2.902GlyPhe: 2.902 ± 0.43
8.401GlyGly: 8.401 ± 1.498
1.375GlyHis: 1.375 ± 0.326
3.36GlyIle: 3.36 ± 0.499
3.513GlyLys: 3.513 ± 0.487
6.568GlyLeu: 6.568 ± 0.676
1.986GlyMet: 1.986 ± 0.373
2.673GlyAsn: 2.673 ± 0.409
3.437GlyPro: 3.437 ± 0.483
2.902GlyGln: 2.902 ± 0.46
6.033GlyArg: 6.033 ± 0.708
5.804GlySer: 5.804 ± 0.93
4.506GlyThr: 4.506 ± 1.034
6.568GlyVal: 6.568 ± 0.973
1.68GlyTrp: 1.68 ± 0.312
2.749GlyTyr: 2.749 ± 0.557
0.0GlyXaa: 0.0 ± 0.0
His
1.68HisAla: 1.68 ± 0.394
0.153HisCys: 0.153 ± 0.105
0.916HisAsp: 0.916 ± 0.231
1.069HisGlu: 1.069 ± 0.235
0.687HisPhe: 0.687 ± 0.2
1.146HisGly: 1.146 ± 0.298
0.305HisHis: 0.305 ± 0.206
0.764HisIle: 0.764 ± 0.226
0.916HisLys: 0.916 ± 0.333
1.146HisLeu: 1.146 ± 0.308
0.076HisMet: 0.076 ± 0.065
0.305HisAsn: 0.305 ± 0.152
1.604HisPro: 1.604 ± 0.312
0.764HisGln: 0.764 ± 0.234
1.604HisArg: 1.604 ± 0.339
0.687HisSer: 0.687 ± 0.188
0.382HisThr: 0.382 ± 0.155
1.146HisVal: 1.146 ± 0.321
0.382HisTrp: 0.382 ± 0.182
0.687HisTyr: 0.687 ± 0.258
0.0HisXaa: 0.0 ± 0.0
Ile
5.957IleAla: 5.957 ± 0.596
0.382IleCys: 0.382 ± 0.169
3.971IleAsp: 3.971 ± 0.625
3.971IleGlu: 3.971 ± 0.496
0.993IlePhe: 0.993 ± 0.214
4.582IleGly: 4.582 ± 0.689
0.611IleHis: 0.611 ± 0.202
2.215IleIle: 2.215 ± 0.372
1.451IleLys: 1.451 ± 0.372
3.742IleLeu: 3.742 ± 0.619
0.84IleMet: 0.84 ± 0.211
1.222IleAsn: 1.222 ± 0.276
2.367IlePro: 2.367 ± 0.288
1.604IleGln: 1.604 ± 0.38
3.131IleArg: 3.131 ± 0.36
2.673IleSer: 2.673 ± 0.377
2.597IleThr: 2.597 ± 0.352
4.048IleVal: 4.048 ± 0.52
0.764IleTrp: 0.764 ± 0.216
0.84IleTyr: 0.84 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
6.262LysAla: 6.262 ± 0.583
0.458LysCys: 0.458 ± 0.182
1.986LysAsp: 1.986 ± 0.398
2.52LysGlu: 2.52 ± 0.39
1.222LysPhe: 1.222 ± 0.221
3.36LysGly: 3.36 ± 0.497
0.305LysHis: 0.305 ± 0.152
2.291LysIle: 2.291 ± 0.354
2.215LysLys: 2.215 ± 0.369
3.742LysLeu: 3.742 ± 0.503
0.687LysMet: 0.687 ± 0.233
1.298LysAsn: 1.298 ± 0.311
2.291LysPro: 2.291 ± 0.456
1.069LysGln: 1.069 ± 0.262
3.208LysArg: 3.208 ± 0.577
2.215LysSer: 2.215 ± 0.541
2.52LysThr: 2.52 ± 0.384
2.597LysVal: 2.597 ± 0.653
0.687LysTrp: 0.687 ± 0.247
1.069LysTyr: 1.069 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
9.546LeuAla: 9.546 ± 0.771
0.993LeuCys: 0.993 ± 0.323
4.888LeuAsp: 4.888 ± 0.516
4.964LeuGlu: 4.964 ± 0.618
2.444LeuPhe: 2.444 ± 0.408
5.728LeuGly: 5.728 ± 0.653
1.604LeuHis: 1.604 ± 0.339
2.902LeuIle: 2.902 ± 0.493
3.36LeuLys: 3.36 ± 0.391
6.644LeuLeu: 6.644 ± 0.89
1.451LeuMet: 1.451 ± 0.307
2.138LeuAsn: 2.138 ± 0.371
3.36LeuPro: 3.36 ± 0.595
3.284LeuGln: 3.284 ± 0.491
6.568LeuArg: 6.568 ± 0.705
6.568LeuSer: 6.568 ± 0.713
5.27LeuThr: 5.27 ± 0.538
6.644LeuVal: 6.644 ± 0.722
1.222LeuTrp: 1.222 ± 0.262
1.68LeuTyr: 1.68 ± 0.341
0.0LeuXaa: 0.0 ± 0.0
Met
2.597MetAla: 2.597 ± 0.412
0.076MetCys: 0.076 ± 0.08
0.84MetAsp: 0.84 ± 0.199
1.069MetGlu: 1.069 ± 0.28
0.84MetPhe: 0.84 ± 0.28
1.298MetGly: 1.298 ± 0.349
0.993MetHis: 0.993 ± 0.245
1.298MetIle: 1.298 ± 0.282
1.375MetLys: 1.375 ± 0.298
1.451MetLeu: 1.451 ± 0.293
0.305MetMet: 0.305 ± 0.15
0.84MetAsn: 0.84 ± 0.234
1.68MetPro: 1.68 ± 0.344
0.993MetGln: 0.993 ± 0.267
1.68MetArg: 1.68 ± 0.384
1.757MetSer: 1.757 ± 0.338
2.138MetThr: 2.138 ± 0.474
0.916MetVal: 0.916 ± 0.313
0.305MetTrp: 0.305 ± 0.13
0.153MetTyr: 0.153 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
2.673AsnAla: 2.673 ± 0.432
0.229AsnCys: 0.229 ± 0.126
1.298AsnAsp: 1.298 ± 0.294
1.146AsnGlu: 1.146 ± 0.309
1.298AsnPhe: 1.298 ± 0.309
3.36AsnGly: 3.36 ± 0.565
0.382AsnHis: 0.382 ± 0.147
1.375AsnIle: 1.375 ± 0.366
1.298AsnLys: 1.298 ± 0.396
2.215AsnLeu: 2.215 ± 0.373
0.382AsnMet: 0.382 ± 0.157
0.84AsnAsn: 0.84 ± 0.256
1.986AsnPro: 1.986 ± 0.36
0.993AsnGln: 0.993 ± 0.247
2.367AsnArg: 2.367 ± 0.395
1.68AsnSer: 1.68 ± 0.398
0.916AsnThr: 0.916 ± 0.263
2.673AsnVal: 2.673 ± 0.572
0.382AsnTrp: 0.382 ± 0.171
0.687AsnTyr: 0.687 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
6.262ProAla: 6.262 ± 0.818
0.458ProCys: 0.458 ± 0.163
3.208ProAsp: 3.208 ± 0.564
3.131ProGlu: 3.131 ± 0.516
1.986ProPhe: 1.986 ± 0.322
4.124ProGly: 4.124 ± 0.557
1.146ProHis: 1.146 ± 0.258
2.367ProIle: 2.367 ± 0.454
1.604ProLys: 1.604 ± 0.308
3.666ProLeu: 3.666 ± 0.609
0.993ProMet: 0.993 ± 0.229
1.757ProAsn: 1.757 ± 0.365
2.749ProPro: 2.749 ± 0.593
1.298ProGln: 1.298 ± 0.334
2.749ProArg: 2.749 ± 0.554
2.902ProSer: 2.902 ± 0.446
3.284ProThr: 3.284 ± 0.482
3.971ProVal: 3.971 ± 0.461
0.84ProTrp: 0.84 ± 0.29
1.375ProTyr: 1.375 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
4.888GlnAla: 4.888 ± 0.7
0.229GlnCys: 0.229 ± 0.179
1.451GlnAsp: 1.451 ± 0.303
1.757GlnGlu: 1.757 ± 0.437
1.375GlnPhe: 1.375 ± 0.305
3.055GlnGly: 3.055 ± 0.589
0.687GlnHis: 0.687 ± 0.237
3.666GlnIle: 3.666 ± 0.603
1.146GlnLys: 1.146 ± 0.281
3.971GlnLeu: 3.971 ± 0.58
0.916GlnMet: 0.916 ± 0.24
1.375GlnAsn: 1.375 ± 0.29
1.451GlnPro: 1.451 ± 0.337
1.909GlnGln: 1.909 ± 0.408
1.986GlnArg: 1.986 ± 0.41
2.215GlnSer: 2.215 ± 0.334
2.138GlnThr: 2.138 ± 0.32
2.673GlnVal: 2.673 ± 0.438
0.764GlnTrp: 0.764 ± 0.261
0.458GlnTyr: 0.458 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
6.721ArgAla: 6.721 ± 0.958
0.687ArgCys: 0.687 ± 0.321
5.27ArgAsp: 5.27 ± 0.509
3.971ArgGlu: 3.971 ± 0.648
2.597ArgPhe: 2.597 ± 0.458
5.346ArgGly: 5.346 ± 0.513
1.833ArgHis: 1.833 ± 0.322
3.437ArgIle: 3.437 ± 0.509
3.895ArgLys: 3.895 ± 0.522
6.262ArgLeu: 6.262 ± 0.654
2.291ArgMet: 2.291 ± 0.374
1.527ArgAsn: 1.527 ± 0.277
3.666ArgPro: 3.666 ± 0.646
2.978ArgGln: 2.978 ± 0.473
7.102ArgArg: 7.102 ± 1.058
3.895ArgSer: 3.895 ± 0.575
3.971ArgThr: 3.971 ± 0.488
5.04ArgVal: 5.04 ± 0.711
1.909ArgTrp: 1.909 ± 0.389
2.291ArgTyr: 2.291 ± 0.409
0.0ArgXaa: 0.0 ± 0.0
Ser
6.415SerAla: 6.415 ± 0.717
0.611SerCys: 0.611 ± 0.176
3.055SerAsp: 3.055 ± 0.502
3.742SerGlu: 3.742 ± 0.625
2.749SerPhe: 2.749 ± 0.617
5.957SerGly: 5.957 ± 1.089
0.611SerHis: 0.611 ± 0.193
3.589SerIle: 3.589 ± 0.478
1.909SerLys: 1.909 ± 0.428
4.124SerLeu: 4.124 ± 0.54
1.298SerMet: 1.298 ± 0.289
2.062SerAsn: 2.062 ± 0.329
2.826SerPro: 2.826 ± 0.471
2.597SerGln: 2.597 ± 0.398
3.284SerArg: 3.284 ± 0.537
3.589SerSer: 3.589 ± 0.652
3.819SerThr: 3.819 ± 0.593
4.353SerVal: 4.353 ± 0.648
0.611SerTrp: 0.611 ± 0.161
0.993SerTyr: 0.993 ± 0.298
0.0SerXaa: 0.0 ± 0.0
Thr
6.644ThrAla: 6.644 ± 0.947
0.382ThrCys: 0.382 ± 0.139
3.437ThrAsp: 3.437 ± 0.507
3.819ThrGlu: 3.819 ± 0.468
2.291ThrPhe: 2.291 ± 0.319
4.964ThrGly: 4.964 ± 0.763
0.611ThrHis: 0.611 ± 0.206
2.749ThrIle: 2.749 ± 0.445
2.291ThrLys: 2.291 ± 0.389
5.346ThrLeu: 5.346 ± 0.664
1.298ThrMet: 1.298 ± 0.295
1.375ThrAsn: 1.375 ± 0.37
3.284ThrPro: 3.284 ± 0.581
1.298ThrGln: 1.298 ± 0.232
4.506ThrArg: 4.506 ± 0.593
3.36ThrSer: 3.36 ± 0.531
3.36ThrThr: 3.36 ± 0.582
4.43ThrVal: 4.43 ± 0.559
1.146ThrTrp: 1.146 ± 0.275
1.604ThrTyr: 1.604 ± 0.261
0.0ThrXaa: 0.0 ± 0.0
Val
7.332ValAla: 7.332 ± 0.915
0.764ValCys: 0.764 ± 0.27
4.43ValAsp: 4.43 ± 0.557
5.651ValGlu: 5.651 ± 0.731
2.597ValPhe: 2.597 ± 0.444
5.499ValGly: 5.499 ± 0.744
1.069ValHis: 1.069 ± 0.315
3.589ValIle: 3.589 ± 0.575
3.666ValLys: 3.666 ± 0.571
3.971ValLeu: 3.971 ± 0.514
1.451ValMet: 1.451 ± 0.345
2.062ValAsn: 2.062 ± 0.476
5.117ValPro: 5.117 ± 0.488
1.909ValGln: 1.909 ± 0.394
5.04ValArg: 5.04 ± 0.526
3.971ValSer: 3.971 ± 0.682
4.964ValThr: 4.964 ± 0.709
4.659ValVal: 4.659 ± 0.681
1.298ValTrp: 1.298 ± 0.371
1.68ValTyr: 1.68 ± 0.391
0.0ValXaa: 0.0 ± 0.0
Trp
1.451TrpAla: 1.451 ± 0.287
0.687TrpCys: 0.687 ± 0.229
0.84TrpAsp: 0.84 ± 0.204
0.916TrpGlu: 0.916 ± 0.316
0.687TrpPhe: 0.687 ± 0.18
0.993TrpGly: 0.993 ± 0.236
0.229TrpHis: 0.229 ± 0.13
0.84TrpIle: 0.84 ± 0.308
0.993TrpLys: 0.993 ± 0.268
2.138TrpLeu: 2.138 ± 0.394
0.153TrpMet: 0.153 ± 0.104
0.687TrpAsn: 0.687 ± 0.227
0.764TrpPro: 0.764 ± 0.314
0.764TrpGln: 0.764 ± 0.232
1.986TrpArg: 1.986 ± 0.349
1.757TrpSer: 1.757 ± 0.357
1.146TrpThr: 1.146 ± 0.293
1.298TrpVal: 1.298 ± 0.274
0.229TrpTrp: 0.229 ± 0.13
0.229TrpTyr: 0.229 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.909TyrAla: 1.909 ± 0.304
0.153TyrCys: 0.153 ± 0.094
1.451TyrAsp: 1.451 ± 0.375
1.375TyrGlu: 1.375 ± 0.303
1.527TyrPhe: 1.527 ± 0.322
2.291TyrGly: 2.291 ± 0.408
0.305TyrHis: 0.305 ± 0.157
0.687TyrIle: 0.687 ± 0.21
0.458TyrLys: 0.458 ± 0.178
1.757TyrLeu: 1.757 ± 0.322
0.229TyrMet: 0.229 ± 0.14
0.535TyrAsn: 0.535 ± 0.211
0.916TyrPro: 0.916 ± 0.242
1.298TyrGln: 1.298 ± 0.305
2.826TyrArg: 2.826 ± 0.511
1.222TyrSer: 1.222 ± 0.392
1.222TyrThr: 1.222 ± 0.272
1.222TyrVal: 1.222 ± 0.33
0.916TyrTrp: 0.916 ± 0.266
0.535TyrTyr: 0.535 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13095 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski