Nocardia asteroides NBRC 15531

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia; Nocardia asteroides

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6459 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5E6P0|U5E6P0_NOCAS Phosphatidate cytidylyltransferase OS=Nocardia asteroides NBRC 15531 OX=1110697 GN=cdsA PE=3 SV=1
MM1 pKa = 7.2LQCASVRR8 pKa = 11.84KK9 pKa = 9.74SSVVLLVVAGLLSGCGDD26 pKa = 4.7DD27 pKa = 6.27DD28 pKa = 6.91DD29 pKa = 7.18DD30 pKa = 5.31DD31 pKa = 6.82AYY33 pKa = 11.45LSSTTITLPTTATPAAVGPVEE54 pKa = 4.03VDD56 pKa = 2.97QGFTGSTVGVEE67 pKa = 4.28LGQVLLVRR75 pKa = 11.84LPQNPDD81 pKa = 3.0DD82 pKa = 4.59HH83 pKa = 6.67GQWTPVNVEE92 pKa = 3.89EE93 pKa = 4.75GVLHH97 pKa = 7.02AEE99 pKa = 4.56PPQSEE104 pKa = 4.48GLSTVYY110 pKa = 10.3PFRR113 pKa = 11.84AVGPGTATLQFAYY126 pKa = 9.99GPAAEE131 pKa = 5.08PPLAPGPEE139 pKa = 4.46FILYY143 pKa = 9.12VQVDD147 pKa = 3.38

Molecular weight: 15.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5ED18|U5ED18_NOCAS RNA polymerase ECF-type sigma factor SigE OS=Nocardia asteroides NBRC 15531 OX=1110697 GN=sigE PE=3 SV=1
MM1 pKa = 7.07LTVAKK6 pKa = 10.12GKK8 pKa = 8.71RR9 pKa = 11.84TFQPNNRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84VHH22 pKa = 5.99GFRR25 pKa = 11.84LRR27 pKa = 11.84MRR29 pKa = 11.84TRR31 pKa = 11.84AGRR34 pKa = 11.84AIVSARR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 9.6GRR45 pKa = 11.84AEE47 pKa = 3.73LTAA50 pKa = 4.95

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6459

0

6459

2104526

42

14596

325.8

34.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.026 ± 0.046

0.72 ± 0.01

6.236 ± 0.028

5.222 ± 0.027

2.947 ± 0.017

9.02 ± 0.032

2.143 ± 0.015

3.967 ± 0.02

1.837 ± 0.025

10.256 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.013

1.854 ± 0.016

5.958 ± 0.032

2.795 ± 0.018

7.555 ± 0.033

4.987 ± 0.019

6.448 ± 0.026

8.753 ± 0.032

1.44 ± 0.013

2.026 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski