Klebsiella phage 3LV2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Reginaelenavirus; Klebsiella virus 3LV2017

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2FAD3|A0A2Z2FAD3_9CAUD Late control D protein OS=Klebsiella phage 3LV2017 OX=1960657 PE=4 SV=1
MM1 pKa = 7.26PTIDD5 pKa = 5.54LSQLPKK11 pKa = 9.14PTIIEE16 pKa = 4.0EE17 pKa = 4.38LDD19 pKa = 3.49FEE21 pKa = 4.95TILVDD26 pKa = 4.08VKK28 pKa = 11.39ALMVAVFPADD38 pKa = 3.43QQSAVAAALGLEE50 pKa = 4.25SEE52 pKa = 4.71PLNIIAQAMAYY63 pKa = 10.08RR64 pKa = 11.84EE65 pKa = 3.97LLLRR69 pKa = 11.84QRR71 pKa = 11.84INEE74 pKa = 3.96AVAACMLSHH83 pKa = 6.33STGDD87 pKa = 3.62DD88 pKa = 3.49LDD90 pKa = 5.39NITANLDD97 pKa = 3.62TEE99 pKa = 4.38RR100 pKa = 11.84LTITSATDD108 pKa = 2.95TTDD111 pKa = 3.34AVTEE115 pKa = 4.0SDD117 pKa = 3.08EE118 pKa = 4.18ALRR121 pKa = 11.84LRR123 pKa = 11.84AQAAFEE129 pKa = 4.23GMSVAGPSAAYY140 pKa = 9.83EE141 pKa = 4.09YY142 pKa = 10.25FARR145 pKa = 11.84SASGKK150 pKa = 9.32VADD153 pKa = 4.76VKK155 pKa = 11.09ASSPAPAEE163 pKa = 3.85VVIAVLSTEE172 pKa = 4.35GDD174 pKa = 3.47GTASPEE180 pKa = 4.0LLADD184 pKa = 3.42VAEE187 pKa = 4.37AVNDD191 pKa = 3.64EE192 pKa = 4.22EE193 pKa = 5.25ARR195 pKa = 11.84PLGDD199 pKa = 2.74RR200 pKa = 11.84VTVRR204 pKa = 11.84SAEE207 pKa = 3.97IVDD210 pKa = 3.71YY211 pKa = 10.59EE212 pKa = 4.38INATLYY218 pKa = 10.24LYY220 pKa = 8.88PGPEE224 pKa = 4.05SEE226 pKa = 5.08PIINAADD233 pKa = 3.51ASLQKK238 pKa = 10.43FLKK241 pKa = 10.38QNNKK245 pKa = 9.96KK246 pKa = 9.96ISRR249 pKa = 11.84DD250 pKa = 3.38VARR253 pKa = 11.84SAISAALHH261 pKa = 4.67VQGVQRR267 pKa = 11.84VVINSPANDD276 pKa = 3.17IKK278 pKa = 11.02ISDD281 pKa = 3.63VQAARR286 pKa = 11.84NIGYY290 pKa = 9.28HH291 pKa = 6.18LEE293 pKa = 4.2NGGTDD298 pKa = 3.39EE299 pKa = 4.6

Molecular weight: 31.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2FB29|A0A2Z2FB29_9CAUD Tail completion protein OS=Klebsiella phage 3LV2017 OX=1960657 PE=4 SV=1
MM1 pKa = 7.36NKK3 pKa = 9.91RR4 pKa = 11.84YY5 pKa = 9.4SQHH8 pKa = 5.85GKK10 pKa = 9.74HH11 pKa = 6.32SGSICSTLLDD21 pKa = 4.18NLPKK25 pKa = 9.69VTWINKK31 pKa = 8.65HH32 pKa = 6.42AGICCGFTIRR42 pKa = 11.84VLPRR46 pKa = 11.84RR47 pKa = 11.84VGKK50 pKa = 10.14KK51 pKa = 9.53RR52 pKa = 11.84YY53 pKa = 9.93QIMKK57 pKa = 9.98DD58 pKa = 3.2GDD60 pKa = 3.78SFGIDD65 pKa = 3.42FALSEE70 pKa = 4.07ARR72 pKa = 11.84KK73 pKa = 8.17TIGRR77 pKa = 11.84IINNNRR83 pKa = 11.84FTIHH87 pKa = 6.38

Molecular weight: 9.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

9654

66

973

268.2

29.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.69 ± 0.674

0.767 ± 0.135

6.049 ± 0.286

6.008 ± 0.378

3.398 ± 0.22

7.81 ± 0.454

1.751 ± 0.212

5.304 ± 0.265

5.003 ± 0.266

8.504 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.766 ± 0.165

3.998 ± 0.302

4.123 ± 0.148

3.936 ± 0.252

6.868 ± 0.399

6.236 ± 0.24

6.329 ± 0.252

5.925 ± 0.267

1.606 ± 0.156

2.931 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski