Eimeria acervulina (Coccidian parasite)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Eimeriidae; Eimeria

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6867 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6GJ12|U6GJ12_EIMAC Uncharacterized protein (Fragment) OS=Eimeria acervulina OX=5801 GN=EAH_00042010 PE=4 SV=1
MM1 pKa = 7.67SEE3 pKa = 4.12SQQGGAYY10 pKa = 8.98SHH12 pKa = 7.36LYY14 pKa = 10.27KK15 pKa = 10.49IILVGDD21 pKa = 3.52ATVGKK26 pKa = 7.42THH28 pKa = 7.32LLSRR32 pKa = 11.84YY33 pKa = 7.01TRR35 pKa = 11.84GSLPVLPKK43 pKa = 9.86ATIGVEE49 pKa = 4.26FVTRR53 pKa = 11.84TVPLAVGGTVKK64 pKa = 10.73AQACHH69 pKa = 6.19PAAAAAAAAAAAPAAAAAAAAAGDD93 pKa = 4.98DD94 pKa = 5.46DD95 pKa = 7.02DD96 pKa = 7.73DD97 pKa = 7.71DD98 pKa = 7.63DD99 pKa = 7.68DD100 pKa = 7.62DD101 pKa = 7.62DD102 pKa = 7.57DD103 pKa = 7.36DD104 pKa = 6.95DD105 pKa = 7.12DD106 pKa = 5.26YY107 pKa = 11.84DD108 pKa = 3.84YY109 pKa = 11.16DD110 pKa = 3.89YY111 pKa = 11.1DD112 pKa = 4.21YY113 pKa = 11.15DD114 pKa = 4.12YY115 pKa = 11.14DD116 pKa = 4.79YY117 pKa = 11.92DD118 pKa = 6.04DD119 pKa = 4.95GDD121 pKa = 3.55YY122 pKa = 10.92DD123 pKa = 4.47YY124 pKa = 10.89DD125 pKa = 3.97YY126 pKa = 11.87ADD128 pKa = 4.53DD129 pKa = 4.87AVVDD133 pKa = 4.18DD134 pKa = 4.62ADD136 pKa = 4.08GNVRR140 pKa = 11.84AAAVAADD147 pKa = 4.21DD148 pKa = 5.26ADD150 pKa = 4.06VADD153 pKa = 3.82VDD155 pKa = 4.08AAAVASTADD164 pKa = 3.84IPPPPPLPPPTAAAAVAAAAAVAADD189 pKa = 4.46MGYY192 pKa = 9.55SWAGALPQHH201 pKa = 6.63NISSLPPCSRR211 pKa = 11.84SFISLL216 pKa = 3.4

Molecular weight: 21.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6GQS1|U6GQS1_EIMAC Uncharacterized protein OS=Eimeria acervulina OX=5801 GN=EAH_00004870 PE=4 SV=1
MM1 pKa = 7.42SSSSSSNSSSNSSTNGKK18 pKa = 7.83TSITSSSSSSTSSSSTSTSSSSRR41 pKa = 11.84SSSSSSSITSTSSSSSTSSSSSTSTSSSTSTSSSTSTSSSSSSTSTSSSRR91 pKa = 11.84STSSSSSSTSSSSSTSSSSSTSTSSSSSSTSSSSSTSSSSSRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84IRR137 pKa = 11.84RR138 pKa = 3.28

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6867

0

6867

4597400

12

10410

669.5

71.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.3 ± 0.098

1.898 ± 0.016

3.586 ± 0.022

6.08 ± 0.034

2.47 ± 0.019

6.285 ± 0.034

2.092 ± 0.013

2.435 ± 0.022

3.466 ± 0.025

9.494 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.327 ± 0.012

2.367 ± 0.016

5.722 ± 0.029

10.369 ± 0.111

5.734 ± 0.023

9.846 ± 0.047

4.475 ± 0.021

4.712 ± 0.028

0.927 ± 0.008

1.415 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski