Tangfeifania diversioriginum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Prolixibacteraceae; Tangfeifania

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6H0C1|A0A1M6H0C1_9BACT Cupin domain-containing protein OS=Tangfeifania diversioriginum OX=1168035 GN=SAMN05444280_11234 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.45KK3 pKa = 10.28LLLSFSVVLIFLFTGMQTTWAQATINVPTDD33 pKa = 3.05QPTIQDD39 pKa = 4.6AITAASANDD48 pKa = 4.2IINVAAGTYY57 pKa = 9.61NEE59 pKa = 4.83SFILDD64 pKa = 3.65KK65 pKa = 11.16TGLTLQGPNEE75 pKa = 4.05ALNGNDD81 pKa = 3.37AGRR84 pKa = 11.84AAEE87 pKa = 4.67AIIEE91 pKa = 4.24GVARR95 pKa = 11.84VEE97 pKa = 4.05ADD99 pKa = 3.35DD100 pKa = 5.04VIIKK104 pKa = 10.6GLVIDD109 pKa = 4.95GANVAQTTNLTMRR122 pKa = 11.84GILVANTNARR132 pKa = 11.84DD133 pKa = 3.53NVTIEE138 pKa = 3.81NNIIKK143 pKa = 10.21NWVTGISLAGSSTFAWVDD161 pKa = 3.16GATITGNLFVNNGVGSTEE179 pKa = 4.08NVDD182 pKa = 3.74GLSITNNAFDD192 pKa = 3.87NGGIGLGGGAVLAVPITGNDD212 pKa = 3.34FSNGSRR218 pKa = 11.84YY219 pKa = 9.45ISAATGVTADD229 pKa = 4.15FDD231 pKa = 4.68AMLSANTFDD240 pKa = 3.39GGAYY244 pKa = 10.27AEE246 pKa = 5.36LITGQWYY253 pKa = 9.63DD254 pKa = 3.23RR255 pKa = 11.84AIFSTIQGAIDD266 pKa = 3.32KK267 pKa = 10.62AGAGSVAKK275 pKa = 10.65VADD278 pKa = 3.49GTYY281 pKa = 10.4NEE283 pKa = 4.9NVTISDD289 pKa = 4.24NISLISEE296 pKa = 4.28NGASSTIIDD305 pKa = 3.91GDD307 pKa = 3.69NSGSEE312 pKa = 4.36LGTILLKK319 pKa = 10.79PGGNGISIGAVGQGFTIKK337 pKa = 10.88GIDD340 pKa = 3.62GPAGLEE346 pKa = 3.59KK347 pKa = 10.55AAIYY351 pKa = 9.14FQGAQDD357 pKa = 4.22NITIEE362 pKa = 4.16GNIIEE367 pKa = 4.53ARR369 pKa = 11.84GDD371 pKa = 3.36AALLGEE377 pKa = 4.43YY378 pKa = 9.99NAANNNITITNNEE391 pKa = 3.58ITGQTFTGANPAGVGFGAQFSLPNVPRR418 pKa = 11.84QLVTFGGGSGTTNTQNFTFTNNIISGIAGGMSITDD453 pKa = 3.76DD454 pKa = 3.65NGNTISPTEE463 pKa = 3.94QGNTMVTLDD472 pKa = 3.84LVGTNVITGNTFSGTTTRR490 pKa = 11.84YY491 pKa = 10.05AYY493 pKa = 10.46ALRR496 pKa = 11.84VRR498 pKa = 11.84GAGAYY503 pKa = 7.73TITEE507 pKa = 4.09NNFTGSYY514 pKa = 9.37PGFIFSDD521 pKa = 4.0SNPITATCNWFGTTDD536 pKa = 3.35EE537 pKa = 4.71SVISSKK543 pKa = 10.14MDD545 pKa = 3.33ASITFIPYY553 pKa = 10.26NISQGGACVGGLNYY567 pKa = 10.44AVNIEE572 pKa = 4.32SPLASSNCGTLDD584 pKa = 3.72VPVTVQDD591 pKa = 4.01FNNVGAISLRR601 pKa = 11.84LNYY604 pKa = 10.56DD605 pKa = 3.62DD606 pKa = 6.12VLLSYY611 pKa = 11.63NDD613 pKa = 3.34VTLNTAIKK621 pKa = 10.38SATQVGEE628 pKa = 3.96TGTQEE633 pKa = 4.32GEE635 pKa = 3.97NKK637 pKa = 10.16GQIRR641 pKa = 11.84LAYY644 pKa = 10.2FGDD647 pKa = 4.03AVTLNDD653 pKa = 4.57DD654 pKa = 3.97DD655 pKa = 5.34VLFTLHH661 pKa = 7.12FDD663 pKa = 3.67ILSSTAGNSTTDD675 pKa = 3.65LTWDD679 pKa = 3.43KK680 pKa = 11.13SSPGYY685 pKa = 10.42CEE687 pKa = 3.74LAGPGGTPVYY697 pKa = 10.05TSTFNDD703 pKa = 3.14VTGVVIPEE711 pKa = 3.94RR712 pKa = 11.84PVKK715 pKa = 10.72NVDD718 pKa = 2.9TGRR721 pKa = 11.84EE722 pKa = 3.82YY723 pKa = 11.28CKK725 pKa = 10.55IQDD728 pKa = 4.95AIDD731 pKa = 3.87ADD733 pKa = 4.04EE734 pKa = 4.55TDD736 pKa = 3.53NGDD739 pKa = 3.85VIEE742 pKa = 4.5VSAGEE747 pKa = 4.13YY748 pKa = 10.13VEE750 pKa = 5.44SVDD753 pKa = 3.71VYY755 pKa = 11.31KK756 pKa = 10.98SVILKK761 pKa = 10.52GADD764 pKa = 3.3KK765 pKa = 11.2NSTFIQAPSTLPAASDD781 pKa = 3.46ALSNIVYY788 pKa = 9.36VHH790 pKa = 6.62GSGISTEE797 pKa = 3.74ISGFTIEE804 pKa = 4.85GPGPSGCGSIGRR816 pKa = 11.84GIFVSDD822 pKa = 3.55GAYY825 pKa = 10.76ANIHH829 pKa = 6.98DD830 pKa = 4.77NNILDD835 pKa = 3.59IRR837 pKa = 11.84DD838 pKa = 3.79NPFSGCQNGIAIQIGRR854 pKa = 11.84NALSSSGTAIIHH866 pKa = 5.78NNIISGYY873 pKa = 7.77QKK875 pKa = 10.87GAIVVDD881 pKa = 3.8NSGSNATITNNTITGTGTTDD901 pKa = 2.72VTAQNGIQVSRR912 pKa = 11.84GATATLGGNTISGNSFHH929 pKa = 7.46EE930 pKa = 5.31EE931 pKa = 3.81GSQWDD936 pKa = 3.41WGACGILLYY945 pKa = 10.71QSGAVSLTGGNNISGNDD962 pKa = 3.51QNYY965 pKa = 8.75YY966 pKa = 10.84AYY968 pKa = 10.34DD969 pKa = 3.41VAGNLILGAEE979 pKa = 4.3TFGASTAPVTFGYY992 pKa = 10.78NIADD996 pKa = 3.69YY997 pKa = 10.44TNQNIDD1003 pKa = 3.07ASQCTFAEE1011 pKa = 4.66GNPASAVLSEE1021 pKa = 4.74LFDD1024 pKa = 4.48IEE1026 pKa = 4.36DD1027 pKa = 4.87RR1028 pKa = 11.84IWHH1031 pKa = 5.99SVDD1034 pKa = 3.3DD1035 pKa = 3.92QTISGFVNIKK1045 pKa = 10.04AGNVYY1050 pKa = 8.47VTHH1053 pKa = 6.77TEE1055 pKa = 3.91SGAKK1059 pKa = 8.36IQYY1062 pKa = 10.2GIDD1065 pKa = 3.32AASVNDD1071 pKa = 3.87VIHH1074 pKa = 6.25VKK1076 pKa = 10.5EE1077 pKa = 4.19GTYY1080 pKa = 10.35SEE1082 pKa = 5.19NITVDD1087 pKa = 3.08KK1088 pKa = 11.09DD1089 pKa = 3.64VTLLGANAGVACDD1102 pKa = 3.64TRR1104 pKa = 11.84GAEE1107 pKa = 4.09STIAGTGGAAVSVASDD1123 pKa = 3.41GVTIDD1128 pKa = 3.61GFEE1131 pKa = 4.01ITNPTGNYY1139 pKa = 9.39AVNSTGNNNLLLQYY1153 pKa = 11.37NNVNDD1158 pKa = 3.82VGTSGTPGNVHH1169 pKa = 6.66AVAISMGSANTDD1181 pKa = 3.16DD1182 pKa = 5.51VKK1184 pKa = 10.82IQHH1187 pKa = 6.1NCISNINNAGNTHH1200 pKa = 6.35SGSGIAVGFSTATTDD1215 pKa = 3.11ITNLYY1220 pKa = 9.24IGYY1223 pKa = 8.29NTISQVNSNNALDD1236 pKa = 3.91YY1237 pKa = 11.25ASGGRR1242 pKa = 11.84GANGIILNVGANSTGKK1258 pKa = 8.41VTDD1261 pKa = 3.97GVVEE1265 pKa = 4.23YY1266 pKa = 9.08NTIHH1270 pKa = 7.15DD1271 pKa = 4.02LSGYY1275 pKa = 7.27WVHH1278 pKa = 6.89GVGLEE1283 pKa = 4.14GNTPGALVQNNLIYY1297 pKa = 10.88NLVSTYY1303 pKa = 10.37TDD1305 pKa = 2.93IPGVGVKK1312 pKa = 10.3VEE1314 pKa = 4.74DD1315 pKa = 3.5NTGAATVSVNNNSFTSLSPGILNAVSGATVNATCNWFAADD1355 pKa = 3.69VTEE1358 pKa = 4.87KK1359 pKa = 11.01SLGDD1363 pKa = 3.5VTVVPWLVDD1372 pKa = 3.31GTNSVASGPGFEE1384 pKa = 4.71PATACVACAISIDD1397 pKa = 3.64AVVTNVSCPGGADD1410 pKa = 3.49DD1411 pKa = 5.16GAIDD1415 pKa = 3.63VTVTNGVSPYY1425 pKa = 8.55TYY1427 pKa = 10.48SWTGPDD1433 pKa = 4.07GFTSPDD1439 pKa = 3.24EE1440 pKa = 5.24DD1441 pKa = 3.76ISGLAAGTYY1450 pKa = 9.97NLLVTADD1457 pKa = 3.82NGCSKK1462 pKa = 10.72EE1463 pKa = 4.0KK1464 pKa = 10.65VVVVGTDD1471 pKa = 3.19ADD1473 pKa = 3.95VTAPTITEE1481 pKa = 4.02ALDD1484 pKa = 3.21VLTEE1488 pKa = 4.13EE1489 pKa = 5.15GCTDD1493 pKa = 3.69ADD1495 pKa = 3.81VPVAKK1500 pKa = 8.37TTVAEE1505 pKa = 4.16LEE1507 pKa = 4.2AMGVIIDD1514 pKa = 5.1DD1515 pKa = 4.0CTLDD1519 pKa = 3.44EE1520 pKa = 4.8NMTVSYY1526 pKa = 10.93SDD1528 pKa = 4.14ASAGTCPVVVTRR1540 pKa = 11.84TYY1542 pKa = 10.86TVTDD1546 pKa = 3.91ASNNSVTVDD1555 pKa = 3.1QTINIEE1561 pKa = 4.38DD1562 pKa = 3.77NTPPSIACPPPIAVKK1577 pKa = 10.4MNDD1580 pKa = 2.96GCEE1583 pKa = 3.83YY1584 pKa = 11.03DD1585 pKa = 3.63PAIGSGIGVATATDD1599 pKa = 3.62NCSDD1603 pKa = 3.87AVDD1606 pKa = 3.55IQISNDD1612 pKa = 2.49ITGNLTEE1619 pKa = 4.8GEE1621 pKa = 4.26NFITWTAVDD1630 pKa = 3.66EE1631 pKa = 4.74CNNSVTCTQKK1641 pKa = 9.23VTVIRR1646 pKa = 11.84NTLSGTVMYY1655 pKa = 10.62NNTAQTPMANVVLKK1669 pKa = 11.06LFDD1672 pKa = 4.61SNDD1675 pKa = 3.6DD1676 pKa = 3.59QVGDD1680 pKa = 3.98DD1681 pKa = 3.8VVTSEE1686 pKa = 4.35TNPGNFEE1693 pKa = 4.06FTGLCAGDD1701 pKa = 3.8YY1702 pKa = 9.97TVVASDD1708 pKa = 3.56TSKK1711 pKa = 10.92VGYY1714 pKa = 10.31INATDD1719 pKa = 3.64AGAANAWGTAGGDD1732 pKa = 3.11IEE1734 pKa = 4.88YY1735 pKa = 10.69VQFLAGDD1742 pKa = 3.66VNDD1745 pKa = 4.07NLLINSTDD1753 pKa = 3.09AQAIQNYY1760 pKa = 8.26FVFGGSFTKK1769 pKa = 10.04PWEE1772 pKa = 3.9YY1773 pKa = 10.25WSAGSVIHH1781 pKa = 6.85SNYY1784 pKa = 10.33NPFSSNAIPPHH1795 pKa = 5.91PAAIEE1800 pKa = 4.13VVISGGDD1807 pKa = 3.51VTHH1810 pKa = 7.5DD1811 pKa = 4.84LYY1813 pKa = 11.59AQATGDD1819 pKa = 3.8FNGSFGPTLKK1829 pKa = 10.73SAGSSVRR1836 pKa = 11.84LSADD1840 pKa = 2.77RR1841 pKa = 11.84NMNVEE1846 pKa = 4.15EE1847 pKa = 4.06NQVFNLPLRR1856 pKa = 11.84SEE1858 pKa = 4.37FAMEE1862 pKa = 4.04VGAVSMILDD1871 pKa = 3.62MPEE1874 pKa = 4.81DD1875 pKa = 4.05VVQVTGVQIAGSEE1888 pKa = 4.49VPVTWAMKK1896 pKa = 10.45DD1897 pKa = 3.13NEE1899 pKa = 5.07LRR1901 pKa = 11.84IGWNSLNPVYY1911 pKa = 10.75VGEE1914 pKa = 4.17NDD1916 pKa = 3.26AFVVLKK1922 pKa = 10.47LQTSANFTEE1931 pKa = 4.62GQLMDD1936 pKa = 3.85IGLVYY1941 pKa = 11.02NPLNEE1946 pKa = 4.13LADD1949 pKa = 4.07GNFEE1953 pKa = 4.76PIEE1956 pKa = 4.1NAALKK1961 pKa = 9.53VARR1964 pKa = 11.84VGNGLTDD1971 pKa = 3.22INEE1974 pKa = 4.59DD1975 pKa = 3.46LDD1977 pKa = 3.75IDD1979 pKa = 4.35KK1980 pKa = 8.13MTFSNYY1986 pKa = 9.15PNPFSEE1992 pKa = 4.56STTLMYY1998 pKa = 8.58TIPVDD2003 pKa = 3.5GKK2005 pKa = 11.33VNISVYY2011 pKa = 10.38NALGQLVTTIVDD2023 pKa = 3.35EE2024 pKa = 4.23NQRR2027 pKa = 11.84TGRR2030 pKa = 11.84YY2031 pKa = 7.14TINNCGDD2038 pKa = 3.47EE2039 pKa = 4.74LQPGMYY2045 pKa = 9.15ISKK2048 pKa = 10.67LMLTNGTEE2056 pKa = 4.41SMVSTIKK2063 pKa = 11.02LNVVKK2068 pKa = 10.82

Molecular weight: 214.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6IEJ3|A0A1M6IEJ3_9BACT Uncharacterized protein OS=Tangfeifania diversioriginum OX=1168035 GN=SAMN05444280_11675 PE=4 SV=1
MM1 pKa = 7.9IITTQQPASSAPRR14 pKa = 11.84KK15 pKa = 7.95KK16 pKa = 8.85TRR18 pKa = 11.84RR19 pKa = 11.84CLCYY23 pKa = 10.37NIRR26 pKa = 11.84EE27 pKa = 4.16NLDD30 pKa = 3.24EE31 pKa = 4.52KK32 pKa = 10.17MKK34 pKa = 10.64EE35 pKa = 3.93MRR37 pKa = 11.84LKK39 pKa = 10.35RR40 pKa = 11.84AIIISAFQADD50 pKa = 4.14YY51 pKa = 11.54QMMVGNVSGPAPGGEE66 pKa = 3.77FRR68 pKa = 11.84AFSPVMLWAMPIVLLSKK85 pKa = 10.6AFSLLGYY92 pKa = 9.22RR93 pKa = 11.84AVYY96 pKa = 9.58LVRR99 pKa = 11.84VLSISLQAEE108 pKa = 3.89SLTYY112 pKa = 10.5HH113 pKa = 6.44NLGHH117 pKa = 6.96RR118 pKa = 11.84PRR120 pKa = 11.84PGNHH124 pKa = 5.89SRR126 pKa = 11.84PDD128 pKa = 3.56PYY130 pKa = 11.47LMSTHH135 pKa = 7.2LYY137 pKa = 7.48MAARR141 pKa = 11.84SFMTVHH147 pKa = 7.18RR148 pKa = 11.84PRR150 pKa = 11.84LSATRR155 pKa = 11.84SKK157 pKa = 9.83RR158 pKa = 11.84TAARR162 pKa = 11.84SACRR166 pKa = 11.84SGSEE170 pKa = 4.18HH171 pKa = 7.28LPISNYY177 pKa = 10.06HH178 pKa = 5.47YY179 pKa = 10.2GYY181 pKa = 9.65PAMLQKK187 pKa = 10.51ISS189 pKa = 3.31

Molecular weight: 21.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4103

0

4103

1425793

26

3828

347.5

39.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.376 ± 0.032

0.764 ± 0.012

5.355 ± 0.033

7.41 ± 0.035

5.371 ± 0.029

6.765 ± 0.036

1.828 ± 0.018

7.298 ± 0.033

7.138 ± 0.049

9.105 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.017

5.848 ± 0.031

3.836 ± 0.02

3.433 ± 0.022

4.015 ± 0.029

6.199 ± 0.033

5.351 ± 0.04

6.347 ± 0.03

1.309 ± 0.018

3.926 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski