Amino acid dipepetide frequency for Tangfeifania diversioriginum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.847AlaAla: 4.847 ± 0.07	0.567AlaCys: 0.567 ± 0.019	3.535AlaAsp: 3.535 ± 0.055	4.884AlaGlu: 4.884 ± 0.064	3.398AlaPhe: 3.398 ± 0.056	5.249AlaGly: 5.249 ± 0.06	1.137AlaHis: 1.137 ± 0.032	4.426AlaIle: 4.426 ± 0.074	3.792AlaLys: 3.792 ± 0.055	5.772AlaLeu: 5.772 ± 0.079	1.526AlaMet: 1.526 ± 0.04	3.014AlaAsn: 3.014 ± 0.051	2.019AlaPro: 2.019 ± 0.045	2.343AlaGln: 2.343 ± 0.045	2.722AlaArg: 2.722 ± 0.052	3.733AlaSer: 3.733 ± 0.055	3.374AlaThr: 3.374 ± 0.065	4.452AlaVal: 4.452 ± 0.055	0.838AlaTrp: 0.838 ± 0.022	2.13AlaTyr: 2.13 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.44CysAla: 0.44 ± 0.019	0.097CysCys: 0.097 ± 0.009	0.429CysAsp: 0.429 ± 0.027	0.5CysGlu: 0.5 ± 0.028	0.41CysPhe: 0.41 ± 0.017	0.648CysGly: 0.648 ± 0.025	0.226CysHis: 0.226 ± 0.018	0.499CysIle: 0.499 ± 0.017	0.459CysLys: 0.459 ± 0.019	0.58CysLeu: 0.58 ± 0.017	0.179CysMet: 0.179 ± 0.011	0.445CysAsn: 0.445 ± 0.018	0.365CysPro: 0.365 ± 0.021	0.236CysGln: 0.236 ± 0.013	0.326CysArg: 0.326 ± 0.018	0.558CysSer: 0.558 ± 0.026	0.436CysThr: 0.436 ± 0.023	0.417CysVal: 0.417 ± 0.019	0.096CysTrp: 0.096 ± 0.008	0.295CysTyr: 0.295 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.379AspAla: 3.379 ± 0.043	0.426AspCys: 0.426 ± 0.023	2.965AspAsp: 2.965 ± 0.055	4.533AspGlu: 4.533 ± 0.063	3.6AspPhe: 3.6 ± 0.054	3.804AspGly: 3.804 ± 0.066	0.812AspHis: 0.812 ± 0.029	3.956AspIle: 3.956 ± 0.055	3.409AspLys: 3.409 ± 0.045	4.855AspLeu: 4.855 ± 0.06	1.175AspMet: 1.175 ± 0.032	2.83AspAsn: 2.83 ± 0.053	1.999AspPro: 1.999 ± 0.034	1.425AspGln: 1.425 ± 0.034	2.014AspArg: 2.014 ± 0.041	3.0AspSer: 3.0 ± 0.056	2.391AspThr: 2.391 ± 0.049	3.449AspVal: 3.449 ± 0.057	0.86AspTrp: 0.86 ± 0.027	2.67AspTyr: 2.67 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	4.431GluAla: 4.431 ± 0.06	0.438GluCys: 0.438 ± 0.022	3.316GluAsp: 3.316 ± 0.047	5.668GluGlu: 5.668 ± 0.075	3.333GluPhe: 3.333 ± 0.054	4.189GluGly: 4.189 ± 0.06	1.098GluHis: 1.098 ± 0.025	6.277GluIle: 6.277 ± 0.074	7.169GluLys: 7.169 ± 0.091	6.871GluLeu: 6.871 ± 0.089	2.191GluMet: 2.191 ± 0.045	5.668GluAsn: 5.668 ± 0.074	2.228GluPro: 2.228 ± 0.046	2.54GluGln: 2.54 ± 0.049	2.822GluArg: 2.822 ± 0.046	3.66GluSer: 3.66 ± 0.056	4.082GluThr: 4.082 ± 0.062	4.542GluVal: 4.542 ± 0.066	0.903GluTrp: 0.903 ± 0.027	2.679GluTyr: 2.679 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.185PheAla: 3.185 ± 0.048	0.415PheCys: 0.415 ± 0.018	3.172PheAsp: 3.172 ± 0.049	3.667PheGlu: 3.667 ± 0.047	2.956PhePhe: 2.956 ± 0.063	3.484PheGly: 3.484 ± 0.049	0.98PheHis: 0.98 ± 0.027	3.89PheIle: 3.89 ± 0.061	2.982PheLys: 2.982 ± 0.044	4.956PheLeu: 4.956 ± 0.075	1.222PheMet: 1.222 ± 0.031	3.125PheAsn: 3.125 ± 0.048	2.021PhePro: 2.021 ± 0.038	1.718PheGln: 1.718 ± 0.035	2.27PheArg: 2.27 ± 0.04	4.453PheSer: 4.453 ± 0.063	3.015PheThr: 3.015 ± 0.049	3.185PheVal: 3.185 ± 0.057	0.75PheTrp: 0.75 ± 0.026	2.26PheTyr: 2.26 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.259GlyAla: 4.259 ± 0.063	0.669GlyCys: 0.669 ± 0.034	3.397GlyAsp: 3.397 ± 0.056	4.834GlyGlu: 4.834 ± 0.063	3.776GlyPhe: 3.776 ± 0.055	4.818GlyGly: 4.818 ± 0.081	1.201GlyHis: 1.201 ± 0.027	5.567GlyIle: 5.567 ± 0.07	5.117GlyLys: 5.117 ± 0.065	5.792GlyLeu: 5.792 ± 0.075	1.803GlyMet: 1.803 ± 0.04	3.895GlyAsn: 3.895 ± 0.068	1.601GlyPro: 1.601 ± 0.033	2.067GlyGln: 2.067 ± 0.037	2.571GlyArg: 2.571 ± 0.051	3.972GlySer: 3.972 ± 0.059	4.058GlyThr: 4.058 ± 0.067	4.3GlyVal: 4.3 ± 0.066	1.036GlyTrp: 1.036 ± 0.033	2.913GlyTyr: 2.913 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.037HisAla: 1.037 ± 0.031	0.172HisCys: 0.172 ± 0.01	0.854HisAsp: 0.854 ± 0.026	1.173HisGlu: 1.173 ± 0.03	1.253HisPhe: 1.253 ± 0.032	1.188HisGly: 1.188 ± 0.027	0.49HisHis: 0.49 ± 0.019	1.252HisIle: 1.252 ± 0.033	1.07HisLys: 1.07 ± 0.034	1.805HisLeu: 1.805 ± 0.039	0.356HisMet: 0.356 ± 0.017	0.95HisAsn: 0.95 ± 0.026	1.089HisPro: 1.089 ± 0.031	0.649HisGln: 0.649 ± 0.019	0.789HisArg: 0.789 ± 0.024	1.162HisSer: 1.162 ± 0.028	0.872HisThr: 0.872 ± 0.022	0.981HisVal: 0.981 ± 0.024	0.302HisTrp: 0.302 ± 0.017	0.836HisTyr: 0.836 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	4.771IleAla: 4.771 ± 0.068	0.586IleCys: 0.586 ± 0.022	4.519IleAsp: 4.519 ± 0.062	5.409IleGlu: 5.409 ± 0.061	3.667IlePhe: 3.667 ± 0.065	4.623IleGly: 4.623 ± 0.073	1.388IleHis: 1.388 ± 0.031	5.204IleIle: 5.204 ± 0.076	4.734IleLys: 4.734 ± 0.078	6.7IleLeu: 6.7 ± 0.084	1.335IleMet: 1.335 ± 0.033	3.864IleAsn: 3.864 ± 0.058	3.249IlePro: 3.249 ± 0.05	2.406IleGln: 2.406 ± 0.042	3.09IleArg: 3.09 ± 0.049	5.365IleSer: 5.365 ± 0.067	4.172IleThr: 4.172 ± 0.06	4.359IleVal: 4.359 ± 0.062	0.805IleTrp: 0.805 ± 0.023	2.728IleTyr: 2.728 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.358LysAla: 4.358 ± 0.072	0.412LysCys: 0.412 ± 0.018	3.221LysAsp: 3.221 ± 0.051	5.484LysGlu: 5.484 ± 0.093	2.851LysPhe: 2.851 ± 0.045	4.084LysGly: 4.084 ± 0.061	1.259LysHis: 1.259 ± 0.031	5.926LysIle: 5.926 ± 0.079	6.241LysLys: 6.241 ± 0.087	6.304LysLeu: 6.304 ± 0.089	2.112LysMet: 2.112 ± 0.045	5.036LysAsn: 5.036 ± 0.066	2.542LysPro: 2.542 ± 0.045	2.465LysGln: 2.465 ± 0.048	2.881LysArg: 2.881 ± 0.05	3.948LysSer: 3.948 ± 0.055	4.179LysThr: 4.179 ± 0.063	4.236LysVal: 4.236 ± 0.061	0.891LysTrp: 0.891 ± 0.025	2.946LysTyr: 2.946 ± 0.053	0.0LysXaa: 0.0 ± 0.0
Leu	6.11LeuAla: 6.11 ± 0.075	0.598LeuCys: 0.598 ± 0.024	4.581LeuAsp: 4.581 ± 0.056	6.074LeuGlu: 6.074 ± 0.076	5.194LeuPhe: 5.194 ± 0.075	5.529LeuGly: 5.529 ± 0.07	1.627LeuHis: 1.627 ± 0.035	6.408LeuIle: 6.408 ± 0.086	7.425LeuLys: 7.425 ± 0.089	8.799LeuLeu: 8.799 ± 0.117	2.059LeuMet: 2.059 ± 0.041	5.46LeuAsn: 5.46 ± 0.065	3.891LeuPro: 3.891 ± 0.053	3.184LeuGln: 3.184 ± 0.052	3.411LeuArg: 3.411 ± 0.06	6.124LeuSer: 6.124 ± 0.072	5.074LeuThr: 5.074 ± 0.07	5.53LeuVal: 5.53 ± 0.07	0.957LeuTrp: 0.957 ± 0.025	3.014LeuTyr: 3.014 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	1.823MetAla: 1.823 ± 0.037	0.14MetCys: 0.14 ± 0.011	1.262MetAsp: 1.262 ± 0.03	1.749MetGlu: 1.749 ± 0.041	0.901MetPhe: 0.901 ± 0.029	1.563MetGly: 1.563 ± 0.034	0.424MetHis: 0.424 ± 0.016	1.486MetIle: 1.486 ± 0.027	2.333MetLys: 2.333 ± 0.043	2.117MetLeu: 2.117 ± 0.042	0.562MetMet: 0.562 ± 0.022	1.476MetAsn: 1.476 ± 0.032	1.008MetPro: 1.008 ± 0.03	0.941MetGln: 0.941 ± 0.028	0.883MetArg: 0.883 ± 0.027	1.157MetSer: 1.157 ± 0.029	1.032MetThr: 1.032 ± 0.029	1.544MetVal: 1.544 ± 0.034	0.214MetTrp: 0.214 ± 0.012	0.654MetTyr: 0.654 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.419AsnAla: 3.419 ± 0.052	0.482AsnCys: 0.482 ± 0.028	2.99AsnAsp: 2.99 ± 0.051	4.29AsnGlu: 4.29 ± 0.055	3.044AsnPhe: 3.044 ± 0.054	3.943AsnGly: 3.943 ± 0.068	1.111AsnHis: 1.111 ± 0.027	4.325AsnIle: 4.325 ± 0.058	3.639AsnLys: 3.639 ± 0.053	5.354AsnLeu: 5.354 ± 0.077	1.251AsnMet: 1.251 ± 0.026	3.396AsnAsn: 3.396 ± 0.056	3.01AsnPro: 3.01 ± 0.05	2.221AsnGln: 2.221 ± 0.045	2.601AsnArg: 2.601 ± 0.045	3.683AsnSer: 3.683 ± 0.066	2.899AsnThr: 2.899 ± 0.058	3.143AsnVal: 3.143 ± 0.048	0.888AsnTrp: 0.888 ± 0.027	2.791AsnTyr: 2.791 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.655ProAla: 2.655 ± 0.05	0.203ProCys: 0.203 ± 0.013	2.803ProAsp: 2.803 ± 0.049	4.09ProGlu: 4.09 ± 0.062	2.062ProPhe: 2.062 ± 0.043	3.166ProGly: 3.166 ± 0.052	0.742ProHis: 0.742 ± 0.022	1.914ProIle: 1.914 ± 0.039	2.021ProLys: 2.021 ± 0.035	3.137ProLeu: 3.137 ± 0.046	0.752ProMet: 0.752 ± 0.024	1.863ProAsn: 1.863 ± 0.034	1.161ProPro: 1.161 ± 0.035	1.338ProGln: 1.338 ± 0.032	1.3ProArg: 1.3 ± 0.029	2.086ProSer: 2.086 ± 0.045	1.691ProThr: 1.691 ± 0.036	3.522ProVal: 3.522 ± 0.052	0.483ProTrp: 0.483 ± 0.018	1.374ProTyr: 1.374 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	1.983GlnAla: 1.983 ± 0.044	0.173GlnCys: 0.173 ± 0.01	1.393GlnAsp: 1.393 ± 0.03	2.266GlnGlu: 2.266 ± 0.046	1.624GlnPhe: 1.624 ± 0.037	1.81GlnGly: 1.81 ± 0.04	0.649GlnHis: 0.649 ± 0.02	2.554GlnIle: 2.554 ± 0.041	3.122GlnLys: 3.122 ± 0.05	3.55GlnLeu: 3.55 ± 0.057	0.901GlnMet: 0.901 ± 0.027	2.336GlnAsn: 2.336 ± 0.036	1.457GlnPro: 1.457 ± 0.029	1.627GlnGln: 1.627 ± 0.047	1.404GlnArg: 1.404 ± 0.034	1.903GlnSer: 1.903 ± 0.039	1.845GlnThr: 1.845 ± 0.04	2.168GlnVal: 2.168 ± 0.043	0.405GlnTrp: 0.405 ± 0.018	1.162GlnTyr: 1.162 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	2.191ArgAla: 2.191 ± 0.037	0.262ArgCys: 0.262 ± 0.013	2.02ArgAsp: 2.02 ± 0.036	3.144ArgGlu: 3.144 ± 0.054	2.399ArgPhe: 2.399 ± 0.044	2.308ArgGly: 2.308 ± 0.047	0.726ArgHis: 0.726 ± 0.021	3.217ArgIle: 3.217 ± 0.059	3.4ArgLys: 3.4 ± 0.064	3.715ArgLeu: 3.715 ± 0.067	1.089ArgMet: 1.089 ± 0.027	2.538ArgAsn: 2.538 ± 0.042	1.342ArgPro: 1.342 ± 0.031	1.459ArgGln: 1.459 ± 0.039	1.718ArgArg: 1.718 ± 0.04	2.089ArgSer: 2.089 ± 0.044	1.908ArgThr: 1.908 ± 0.033	2.382ArgVal: 2.382 ± 0.044	0.543ArgTrp: 0.543 ± 0.018	1.694ArgTyr: 1.694 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	4.0SerAla: 4.0 ± 0.062	0.585SerCys: 0.585 ± 0.02	3.498SerAsp: 3.498 ± 0.054	4.52SerGlu: 4.52 ± 0.067	3.554SerPhe: 3.554 ± 0.052	5.204SerGly: 5.204 ± 0.081	1.139SerHis: 1.139 ± 0.029	4.156SerIle: 4.156 ± 0.059	3.686SerLys: 3.686 ± 0.061	5.451SerLeu: 5.451 ± 0.074	1.308SerMet: 1.308 ± 0.031	3.103SerAsn: 3.103 ± 0.046	2.434SerPro: 2.434 ± 0.045	2.052SerGln: 2.052 ± 0.046	2.578SerArg: 2.578 ± 0.05	3.905SerSer: 3.905 ± 0.076	3.105SerThr: 3.105 ± 0.056	4.395SerVal: 4.395 ± 0.066	0.863SerTrp: 0.863 ± 0.025	2.454SerTyr: 2.454 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	3.594ThrAla: 3.594 ± 0.06	0.377ThrCys: 0.377 ± 0.023	3.195ThrAsp: 3.195 ± 0.064	3.736ThrGlu: 3.736 ± 0.048	2.751ThrPhe: 2.751 ± 0.043	4.836ThrGly: 4.836 ± 0.074	1.002ThrHis: 1.002 ± 0.029	4.086ThrIle: 4.086 ± 0.064	2.832ThrLys: 2.832 ± 0.049	4.674ThrLeu: 4.674 ± 0.064	0.931ThrMet: 0.931 ± 0.027	2.716ThrAsn: 2.716 ± 0.047	2.403ThrPro: 2.403 ± 0.049	1.692ThrGln: 1.692 ± 0.036	2.047ThrArg: 2.047 ± 0.038	3.316ThrSer: 3.316 ± 0.047	3.043ThrThr: 3.043 ± 0.071	3.663ThrVal: 3.663 ± 0.082	0.704ThrTrp: 0.704 ± 0.026	1.914ThrTyr: 1.914 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	4.28ValAla: 4.28 ± 0.06	0.57ValCys: 0.57 ± 0.025	3.485ValAsp: 3.485 ± 0.061	4.651ValGlu: 4.651 ± 0.066	3.628ValPhe: 3.628 ± 0.054	3.657ValGly: 3.657 ± 0.058	1.097ValHis: 1.097 ± 0.031	4.527ValIle: 4.527 ± 0.062	4.507ValLys: 4.507 ± 0.06	5.809ValLeu: 5.809 ± 0.069	1.36ValMet: 1.36 ± 0.035	3.483ValAsn: 3.483 ± 0.052	2.51ValPro: 2.51 ± 0.049	1.94ValGln: 1.94 ± 0.035	2.294ValArg: 2.294 ± 0.045	4.327ValSer: 4.327 ± 0.062	3.645ValThr: 3.645 ± 0.087	4.513ValVal: 4.513 ± 0.07	0.767ValTrp: 0.767 ± 0.027	2.416ValTyr: 2.416 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	0.766TrpAla: 0.766 ± 0.024	0.112TrpCys: 0.112 ± 0.01	0.753TrpAsp: 0.753 ± 0.024	0.957TrpGlu: 0.957 ± 0.026	0.706TrpPhe: 0.706 ± 0.021	0.993TrpGly: 0.993 ± 0.027	0.308TrpHis: 0.308 ± 0.016	0.849TrpIle: 0.849 ± 0.025	0.969TrpLys: 0.969 ± 0.029	1.234TrpLeu: 1.234 ± 0.033	0.343TrpMet: 0.343 ± 0.018	0.81TrpAsn: 0.81 ± 0.028	0.387TrpPro: 0.387 ± 0.018	0.543TrpGln: 0.543 ± 0.023	0.511TrpArg: 0.511 ± 0.021	0.741TrpSer: 0.741 ± 0.022	0.621TrpThr: 0.621 ± 0.023	0.758TrpVal: 0.758 ± 0.025	0.233TrpTrp: 0.233 ± 0.015	0.494TrpTyr: 0.494 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.232TyrAla: 2.232 ± 0.037	0.355TyrCys: 0.355 ± 0.014	2.167TyrAsp: 2.167 ± 0.042	2.475TyrGlu: 2.475 ± 0.047	2.599TyrPhe: 2.599 ± 0.039	2.552TyrGly: 2.552 ± 0.043	0.862TyrHis: 0.862 ± 0.025	2.457TyrIle: 2.457 ± 0.038	2.468TyrLys: 2.468 ± 0.044	3.572TyrLeu: 3.572 ± 0.052	0.812TyrMet: 0.812 ± 0.022	2.474TyrAsn: 2.474 ± 0.053	1.648TyrPro: 1.648 ± 0.039	1.487TyrGln: 1.487 ± 0.035	1.914TyrArg: 1.914 ± 0.041	2.807TyrSer: 2.807 ± 0.053	2.066TyrThr: 2.066 ± 0.05	1.929TyrVal: 1.929 ± 0.039	0.551TyrTrp: 0.551 ± 0.019	1.838TyrTyr: 1.838 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4103 proteins (1425793 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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