Terriglobus roseus (strain DSM 18391 / NRRL B-41598 / KBS 63)

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Terriglobus; Terriglobus roseus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3936 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3ZC98|I3ZC98_TERRK Uncharacterized protein OS=Terriglobus roseus (strain DSM 18391 / NRRL B-41598 / KBS 63) OX=926566 GN=Terro_0525 PE=4 SV=1
MM1 pKa = 6.94TQQEE5 pKa = 4.2QQLLNGLIDD14 pKa = 3.84RR15 pKa = 11.84VNGTQLGSKK24 pKa = 10.39DD25 pKa = 3.61EE26 pKa = 4.1EE27 pKa = 5.12AEE29 pKa = 4.06ALLKK33 pKa = 9.91RR34 pKa = 11.84TLGSNPDD41 pKa = 3.41ALYY44 pKa = 10.28ILCQTVLVQGFAMDD58 pKa = 5.19KK59 pKa = 10.34SQQDD63 pKa = 3.41LAAARR68 pKa = 11.84SEE70 pKa = 3.76IDD72 pKa = 4.17ALRR75 pKa = 11.84QSQPEE80 pKa = 3.91KK81 pKa = 10.51HH82 pKa = 6.36GSFLGNLFGLGKK94 pKa = 10.0DD95 pKa = 3.96DD96 pKa = 5.47QPQQTAPTNAGPGSTNTPGYY116 pKa = 9.23NAPSYY121 pKa = 11.19APVANAPAVQQQGYY135 pKa = 9.38QGQQGYY141 pKa = 7.88PAQPGYY147 pKa = 10.15GQPAYY152 pKa = 9.32GQPGYY157 pKa = 9.53PPQPGYY163 pKa = 11.19GYY165 pKa = 10.58GGQPMGGGMFGGGGGFLQGAMQTAAGVVAGEE196 pKa = 3.93FAFRR200 pKa = 11.84AIQDD204 pKa = 3.79VFSGGHH210 pKa = 5.47GGEE213 pKa = 4.34HH214 pKa = 6.62GFGGGTEE221 pKa = 4.32VVNNYY226 pKa = 9.81YY227 pKa = 11.02DD228 pKa = 3.59NTSDD232 pKa = 3.55SGSGGFGDD240 pKa = 5.49RR241 pKa = 11.84LAEE244 pKa = 4.2ANNYY248 pKa = 9.32DD249 pKa = 3.65GSVSSDD255 pKa = 2.79IEE257 pKa = 3.93DD258 pKa = 3.46RR259 pKa = 11.84RR260 pKa = 11.84GEE262 pKa = 3.92GHH264 pKa = 6.77GFFGSGDD271 pKa = 3.46DD272 pKa = 4.05SANNGSNFADD282 pKa = 3.84DD283 pKa = 4.52SGNFDD288 pKa = 4.42AGSDD292 pKa = 3.69DD293 pKa = 4.15GGGFDD298 pKa = 5.38GGDD301 pKa = 3.38SGGGDD306 pKa = 3.84DD307 pKa = 5.48NNYY310 pKa = 10.55

Molecular weight: 31.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3ZMX7|I3ZMX7_TERRK 50S ribosomal protein L34 OS=Terriglobus roseus (strain DSM 18391 / NRRL B-41598 / KBS 63) OX=926566 GN=rpmH PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.87THH17 pKa = 5.78GFLTRR22 pKa = 11.84MKK24 pKa = 9.5TKK26 pKa = 10.57AGQNVLNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.03GRR40 pKa = 11.84HH41 pKa = 5.72KK42 pKa = 10.29IAVSAGFRR50 pKa = 11.84DD51 pKa = 3.66

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3936

0

3936

1413377

21

2214

359.1

39.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.391 ± 0.051

0.844 ± 0.013

5.159 ± 0.033

5.102 ± 0.048

3.789 ± 0.027

8.144 ± 0.042

2.22 ± 0.019

4.764 ± 0.029

3.609 ± 0.035

9.873 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.02

3.348 ± 0.037

5.337 ± 0.028

3.798 ± 0.031

6.213 ± 0.034

6.273 ± 0.034

6.471 ± 0.049

7.328 ± 0.032

1.291 ± 0.015

2.661 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski