Amino acid dipepetide frequency for Volvox carteri f. nagariensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	32.837AlaAla: 32.837 ± 0.253	1.7AlaCys: 1.7 ± 0.019	5.339AlaAsp: 5.339 ± 0.032	6.526AlaGlu: 6.526 ± 0.065	2.733AlaPhe: 2.733 ± 0.021	11.623AlaGly: 11.623 ± 0.073	1.979AlaHis: 1.979 ± 0.018	3.139AlaIle: 3.139 ± 0.026	3.301AlaLys: 3.301 ± 0.026	10.52AlaLeu: 10.52 ± 0.052	2.534AlaMet: 2.534 ± 0.02	2.61AlaAsn: 2.61 ± 0.022	7.427AlaPro: 7.427 ± 0.053	4.047AlaGln: 4.047 ± 0.033	6.635AlaArg: 6.635 ± 0.037	9.134AlaSer: 9.134 ± 0.05	7.672AlaThr: 7.672 ± 0.047	10.075AlaVal: 10.075 ± 0.053	1.228AlaTrp: 1.228 ± 0.015	1.905AlaTyr: 1.905 ± 0.021	0.002AlaXaa: 0.002 ± 0.0
Cys	1.439CysAla: 1.439 ± 0.018	0.534CysCys: 0.534 ± 0.011	0.742CysAsp: 0.742 ± 0.013	0.651CysGlu: 0.651 ± 0.01	0.508CysPhe: 0.508 ± 0.009	1.643CysGly: 1.643 ± 0.021	0.388CysHis: 0.388 ± 0.009	0.573CysIle: 0.573 ± 0.01	0.579CysLys: 0.579 ± 0.012	1.458CysLeu: 1.458 ± 0.016	0.393CysMet: 0.393 ± 0.007	0.544CysAsn: 0.544 ± 0.01	0.96CysPro: 0.96 ± 0.014	0.512CysGln: 0.512 ± 0.008	1.301CysArg: 1.301 ± 0.016	1.435CysSer: 1.435 ± 0.014	1.065CysThr: 1.065 ± 0.015	1.09CysVal: 1.09 ± 0.013	0.309CysTrp: 0.309 ± 0.009	0.372CysTyr: 0.372 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	5.263AspAla: 5.263 ± 0.032	0.765AspCys: 0.765 ± 0.013	3.362AspAsp: 3.362 ± 0.039	3.037AspGlu: 3.037 ± 0.027	1.368AspPhe: 1.368 ± 0.015	5.239AspGly: 5.239 ± 0.033	0.82AspHis: 0.82 ± 0.012	1.609AspIle: 1.609 ± 0.017	1.524AspLys: 1.524 ± 0.02	4.255AspLeu: 4.255 ± 0.025	1.103AspMet: 1.103 ± 0.014	1.236AspAsn: 1.236 ± 0.016	2.929AspPro: 2.929 ± 0.022	1.321AspGln: 1.321 ± 0.015	2.594AspArg: 2.594 ± 0.025	3.056AspSer: 3.056 ± 0.022	2.282AspThr: 2.282 ± 0.019	3.72AspVal: 3.72 ± 0.03	0.659AspTrp: 0.659 ± 0.01	1.007AspTyr: 1.007 ± 0.013	0.0AspXaa: 0.0 ± 0.0
Glu	7.14GluAla: 7.14 ± 0.066	0.627GluCys: 0.627 ± 0.01	3.033GluAsp: 3.033 ± 0.026	4.843GluGlu: 4.843 ± 0.055	1.161GluPhe: 1.161 ± 0.015	4.399GluGly: 4.399 ± 0.031	1.053GluHis: 1.053 ± 0.012	1.34GluIle: 1.34 ± 0.016	1.671GluLys: 1.671 ± 0.021	5.494GluLeu: 5.494 ± 0.041	1.153GluMet: 1.153 ± 0.014	0.995GluAsn: 0.995 ± 0.014	2.677GluPro: 2.677 ± 0.024	2.582GluGln: 2.582 ± 0.026	3.696GluArg: 3.696 ± 0.034	2.58GluSer: 2.58 ± 0.022	1.975GluThr: 1.975 ± 0.016	3.964GluVal: 3.964 ± 0.026	0.648GluTrp: 0.648 ± 0.01	1.079GluTyr: 1.079 ± 0.013	0.0GluXaa: 0.0 ± 0.0
Phe	2.335PheAla: 2.335 ± 0.018	0.515PheCys: 0.515 ± 0.009	1.317PheAsp: 1.317 ± 0.014	1.239PheGlu: 1.239 ± 0.014	0.899PhePhe: 0.899 ± 0.013	2.244PheGly: 2.244 ± 0.021	0.563PheHis: 0.563 ± 0.008	0.891PheIle: 0.891 ± 0.011	0.992PheLys: 0.992 ± 0.014	2.376PheLeu: 2.376 ± 0.02	0.585PheMet: 0.585 ± 0.009	0.894PheAsn: 0.894 ± 0.011	1.41PhePro: 1.41 ± 0.013	0.917PheGln: 0.917 ± 0.012	1.625PheArg: 1.625 ± 0.016	1.881PheSer: 1.881 ± 0.016	1.522PheThr: 1.522 ± 0.017	1.764PheVal: 1.764 ± 0.019	0.352PheTrp: 0.352 ± 0.007	0.604PheTyr: 0.604 ± 0.01	0.0PheXaa: 0.0 ± 0.0
Gly	10.654GlyAla: 10.654 ± 0.067	1.658GlyCys: 1.658 ± 0.02	4.683GlyAsp: 4.683 ± 0.036	4.097GlyGlu: 4.097 ± 0.032	2.195GlyPhe: 2.195 ± 0.02	17.738GlyGly: 17.738 ± 0.153	1.822GlyHis: 1.822 ± 0.021	2.416GlyIle: 2.416 ± 0.018	2.643GlyLys: 2.643 ± 0.026	7.081GlyLeu: 7.081 ± 0.033	1.834GlyMet: 1.834 ± 0.022	2.405GlyAsn: 2.405 ± 0.022	5.423GlyPro: 5.423 ± 0.038	2.893GlyGln: 2.893 ± 0.019	6.14GlyArg: 6.14 ± 0.036	8.51GlySer: 8.51 ± 0.054	4.735GlyThr: 4.735 ± 0.033	5.739GlyVal: 5.739 ± 0.035	1.115GlyTrp: 1.115 ± 0.015	1.679GlyTyr: 1.679 ± 0.019	0.0GlyXaa: 0.0 ± 0.0
His	2.096HisAla: 2.096 ± 0.019	0.395HisCys: 0.395 ± 0.009	0.934HisAsp: 0.934 ± 0.013	0.932HisGlu: 0.932 ± 0.01	0.631HisPhe: 0.631 ± 0.011	1.953HisGly: 1.953 ± 0.019	1.085HisHis: 1.085 ± 0.021	0.802HisIle: 0.802 ± 0.012	0.628HisLys: 0.628 ± 0.01	2.254HisLeu: 2.254 ± 0.021	0.583HisMet: 0.583 ± 0.013	0.646HisAsn: 0.646 ± 0.011	1.647HisPro: 1.647 ± 0.017	1.242HisGln: 1.242 ± 0.018	1.67HisArg: 1.67 ± 0.019	1.56HisSer: 1.56 ± 0.016	1.147HisThr: 1.147 ± 0.014	1.573HisVal: 1.573 ± 0.017	0.306HisTrp: 0.306 ± 0.007	0.518HisTyr: 0.518 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	3.005IleAla: 3.005 ± 0.025	0.654IleCys: 0.654 ± 0.014	1.348IleAsp: 1.348 ± 0.014	1.311IleGlu: 1.311 ± 0.016	0.882IlePhe: 0.882 ± 0.014	1.968IleGly: 1.968 ± 0.017	0.677IleHis: 0.677 ± 0.01	1.138IleIle: 1.138 ± 0.021	1.152IleLys: 1.152 ± 0.015	2.36IleLeu: 2.36 ± 0.023	0.686IleMet: 0.686 ± 0.011	1.008IleAsn: 1.008 ± 0.015	1.639IlePro: 1.639 ± 0.016	1.016IleGln: 1.016 ± 0.012	1.949IleArg: 1.949 ± 0.018	2.291IleSer: 2.291 ± 0.019	1.877IleThr: 1.877 ± 0.02	1.829IleVal: 1.829 ± 0.017	0.356IleTrp: 0.356 ± 0.007	0.664IleTyr: 0.664 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.558LysAla: 3.558 ± 0.031	0.426LysCys: 0.426 ± 0.009	1.507LysAsp: 1.507 ± 0.019	1.956LysGlu: 1.956 ± 0.024	0.744LysPhe: 0.744 ± 0.013	2.248LysGly: 2.248 ± 0.025	0.728LysHis: 0.728 ± 0.013	0.922LysIle: 0.922 ± 0.013	1.563LysLys: 1.563 ± 0.023	2.975LysLeu: 2.975 ± 0.022	0.681LysMet: 0.681 ± 0.011	0.764LysAsn: 0.764 ± 0.011	1.885LysPro: 1.885 ± 0.021	1.489LysGln: 1.489 ± 0.015	2.221LysArg: 2.221 ± 0.024	1.811LysSer: 1.811 ± 0.019	1.438LysThr: 1.438 ± 0.02	2.136LysVal: 2.136 ± 0.023	0.385LysTrp: 0.385 ± 0.011	0.819LysTyr: 0.819 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	10.64LeuAla: 10.64 ± 0.057	1.436LeuCys: 1.436 ± 0.013	4.424LeuAsp: 4.424 ± 0.028	5.268LeuGlu: 5.268 ± 0.038	2.251LeuPhe: 2.251 ± 0.021	6.805LeuGly: 6.805 ± 0.039	2.408LeuHis: 2.408 ± 0.02	2.193LeuIle: 2.193 ± 0.021	2.916LeuLys: 2.916 ± 0.027	10.364LeuLeu: 10.364 ± 0.057	1.926LeuMet: 1.926 ± 0.017	2.14LeuAsn: 2.14 ± 0.021	6.967LeuPro: 6.967 ± 0.038	5.118LeuGln: 5.118 ± 0.034	6.935LeuArg: 6.935 ± 0.041	6.535LeuSer: 6.535 ± 0.034	4.56LeuThr: 4.56 ± 0.028	6.165LeuVal: 6.165 ± 0.043	1.132LeuTrp: 1.132 ± 0.016	2.082LeuTyr: 2.082 ± 0.017	0.001LeuXaa: 0.001 ± 0.0
Met	2.715MetAla: 2.715 ± 0.021	0.347MetCys: 0.347 ± 0.01	1.073MetAsp: 1.073 ± 0.011	1.23MetGlu: 1.23 ± 0.014	0.556MetPhe: 0.556 ± 0.01	1.569MetGly: 1.569 ± 0.02	0.489MetHis: 0.489 ± 0.008	0.498MetIle: 0.498 ± 0.009	0.612MetLys: 0.612 ± 0.009	2.03MetLeu: 2.03 ± 0.019	0.549MetMet: 0.549 ± 0.012	0.47MetAsn: 0.47 ± 0.008	1.409MetPro: 1.409 ± 0.015	1.028MetGln: 1.028 ± 0.012	1.375MetArg: 1.375 ± 0.013	1.502MetSer: 1.502 ± 0.013	1.043MetThr: 1.043 ± 0.012	1.389MetVal: 1.389 ± 0.023	0.274MetTrp: 0.274 ± 0.007	0.588MetTyr: 0.588 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.497AsnAla: 2.497 ± 0.022	0.501AsnCys: 0.501 ± 0.01	1.164AsnAsp: 1.164 ± 0.013	1.077AsnGlu: 1.077 ± 0.014	0.76AsnPhe: 0.76 ± 0.012	2.504AsnGly: 2.504 ± 0.024	0.547AsnHis: 0.547 ± 0.009	1.025AsnIle: 1.025 ± 0.017	0.914AsnLys: 0.914 ± 0.012	2.217AsnLeu: 2.217 ± 0.018	0.588AsnMet: 0.588 ± 0.009	1.031AsnAsn: 1.031 ± 0.018	1.69AsnPro: 1.69 ± 0.016	0.841AsnGln: 0.841 ± 0.012	1.499AsnArg: 1.499 ± 0.017	1.9AsnSer: 1.9 ± 0.021	1.557AsnThr: 1.557 ± 0.02	1.749AsnVal: 1.749 ± 0.019	0.324AsnTrp: 0.324 ± 0.007	0.587AsnTyr: 0.587 ± 0.01	0.0AsnXaa: 0.0 ± 0.0
Pro	8.336ProAla: 8.336 ± 0.057	0.917ProCys: 0.917 ± 0.013	2.828ProAsp: 2.828 ± 0.022	3.243ProGlu: 3.243 ± 0.026	1.516ProPhe: 1.516 ± 0.015	5.93ProGly: 5.93 ± 0.043	1.572ProHis: 1.572 ± 0.017	1.443ProIle: 1.443 ± 0.014	1.669ProLys: 1.669 ± 0.025	6.012ProLeu: 6.012 ± 0.034	1.044ProMet: 1.044 ± 0.015	1.523ProAsn: 1.523 ± 0.015	10.42ProPro: 10.42 ± 0.145	3.113ProGln: 3.113 ± 0.035	4.123ProArg: 4.123 ± 0.028	6.519ProSer: 6.519 ± 0.067	3.468ProThr: 3.468 ± 0.028	4.029ProVal: 4.029 ± 0.03	0.792ProTrp: 0.792 ± 0.011	1.556ProTyr: 1.556 ± 0.016	0.001ProXaa: 0.001 ± 0.0
Gln	4.467GlnAla: 4.467 ± 0.035	0.503GlnCys: 0.503 ± 0.008	1.58GlnAsp: 1.58 ± 0.015	2.314GlnGlu: 2.314 ± 0.025	0.845GlnPhe: 0.845 ± 0.011	2.802GlnGly: 2.802 ± 0.02	1.595GlnHis: 1.595 ± 0.023	1.011GlnIle: 1.011 ± 0.014	1.143GlnLys: 1.143 ± 0.015	5.262GlnLeu: 5.262 ± 0.032	0.835GlnMet: 0.835 ± 0.012	0.788GlnAsn: 0.788 ± 0.01	3.698GlnPro: 3.698 ± 0.042	6.506GlnGln: 6.506 ± 0.102	3.485GlnArg: 3.485 ± 0.03	2.336GlnSer: 2.336 ± 0.022	1.53GlnThr: 1.53 ± 0.015	2.569GlnVal: 2.569 ± 0.017	0.459GlnTrp: 0.459 ± 0.009	0.894GlnTyr: 0.894 ± 0.013	0.001GlnXaa: 0.001 ± 0.0
Arg	6.533ArgAla: 6.533 ± 0.039	1.207ArgCys: 1.207 ± 0.013	3.039ArgAsp: 3.039 ± 0.025	3.505ArgGlu: 3.505 ± 0.031	1.69ArgPhe: 1.69 ± 0.016	5.652ArgGly: 5.652 ± 0.037	1.838ArgHis: 1.838 ± 0.019	1.999ArgIle: 1.999 ± 0.021	2.219ArgLys: 2.219 ± 0.021	6.78ArgLeu: 6.78 ± 0.04	1.44ArgMet: 1.44 ± 0.017	1.782ArgAsn: 1.782 ± 0.016	4.279ArgPro: 4.279 ± 0.026	3.461ArgGln: 3.461 ± 0.03	6.437ArgArg: 6.437 ± 0.045	4.929ArgSer: 4.929 ± 0.03	3.397ArgThr: 3.397 ± 0.022	4.228ArgVal: 4.228 ± 0.027	0.928ArgTrp: 0.928 ± 0.01	1.4ArgTyr: 1.4 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	8.848SerAla: 8.848 ± 0.047	1.48SerCys: 1.48 ± 0.019	3.158SerAsp: 3.158 ± 0.025	3.143SerGlu: 3.143 ± 0.021	1.962SerPhe: 1.962 ± 0.019	8.267SerGly: 8.267 ± 0.05	1.554SerHis: 1.554 ± 0.017	2.099SerIle: 2.099 ± 0.02	2.136SerLys: 2.136 ± 0.02	6.339SerLeu: 6.339 ± 0.034	1.4SerMet: 1.4 ± 0.014	2.001SerAsn: 2.001 ± 0.018	5.899SerPro: 5.899 ± 0.07	2.656SerGln: 2.656 ± 0.022	5.01SerArg: 5.01 ± 0.03	8.933SerSer: 8.933 ± 0.078	4.475SerThr: 4.475 ± 0.031	4.355SerVal: 4.355 ± 0.026	1.001SerTrp: 1.001 ± 0.012	1.732SerTyr: 1.732 ± 0.017	0.001SerXaa: 0.001 ± 0.0
Thr	8.694ThrAla: 8.694 ± 0.055	0.955ThrCys: 0.955 ± 0.015	2.248ThrAsp: 2.248 ± 0.018	2.312ThrGlu: 2.312 ± 0.02	1.438ThrPhe: 1.438 ± 0.016	4.594ThrGly: 4.594 ± 0.025	1.108ThrHis: 1.108 ± 0.014	1.659ThrIle: 1.659 ± 0.024	1.406ThrLys: 1.406 ± 0.022	4.35ThrLeu: 4.35 ± 0.028	0.956ThrMet: 0.956 ± 0.011	1.337ThrAsn: 1.337 ± 0.018	3.614ThrPro: 3.614 ± 0.031	1.735ThrGln: 1.735 ± 0.014	2.937ThrArg: 2.937 ± 0.021	4.551ThrSer: 4.551 ± 0.03	3.811ThrThr: 3.811 ± 0.041	3.583ThrVal: 3.583 ± 0.028	0.669ThrTrp: 0.669 ± 0.01	1.11ThrTyr: 1.11 ± 0.015	0.001ThrXaa: 0.001 ± 0.0
Val	9.082ValAla: 9.082 ± 0.053	1.171ValCys: 1.171 ± 0.015	3.237ValAsp: 3.237 ± 0.02	3.723ValGlu: 3.723 ± 0.028	1.788ValPhe: 1.788 ± 0.018	5.093ValGly: 5.093 ± 0.033	1.549ValHis: 1.549 ± 0.017	1.922ValIle: 1.922 ± 0.016	2.055ValLys: 2.055 ± 0.019	6.907ValLeu: 6.907 ± 0.049	1.621ValMet: 1.621 ± 0.021	1.638ValAsn: 1.638 ± 0.017	4.341ValPro: 4.341 ± 0.033	2.799ValGln: 2.799 ± 0.02	4.577ValArg: 4.577 ± 0.029	4.624ValSer: 4.624 ± 0.029	3.739ValThr: 3.739 ± 0.026	5.753ValVal: 5.753 ± 0.048	0.851ValTrp: 0.851 ± 0.012	1.475ValTyr: 1.475 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.149TrpAla: 1.149 ± 0.012	0.238TrpCys: 0.238 ± 0.006	0.656TrpAsp: 0.656 ± 0.013	0.661TrpGlu: 0.661 ± 0.01	0.331TrpPhe: 0.331 ± 0.007	0.935TrpGly: 0.935 ± 0.012	0.314TrpHis: 0.314 ± 0.007	0.354TrpIle: 0.354 ± 0.006	0.375TrpLys: 0.375 ± 0.008	1.338TrpLeu: 1.338 ± 0.016	0.294TrpMet: 0.294 ± 0.007	0.359TrpAsn: 0.359 ± 0.009	0.707TrpPro: 0.707 ± 0.011	0.6TrpGln: 0.6 ± 0.011	1.147TrpArg: 1.147 ± 0.014	0.879TrpSer: 0.879 ± 0.014	0.589TrpThr: 0.589 ± 0.008	0.825TrpVal: 0.825 ± 0.012	0.245TrpTrp: 0.245 ± 0.007	0.326TrpTyr: 0.326 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.673TyrAla: 1.673 ± 0.02	0.47TyrCys: 0.47 ± 0.011	1.479TyrAsp: 1.479 ± 0.015	1.043TyrGlu: 1.043 ± 0.015	0.668TyrPhe: 0.668 ± 0.01	2.255TyrGly: 2.255 ± 0.023	0.512TyrHis: 0.512 ± 0.011	0.78TyrIle: 0.78 ± 0.015	0.761TyrLys: 0.761 ± 0.013	1.894TyrLeu: 1.894 ± 0.02	0.54TyrMet: 0.54 ± 0.009	0.803TyrAsn: 0.803 ± 0.013	1.028TyrPro: 1.028 ± 0.013	0.744TyrGln: 0.744 ± 0.011	1.383TyrArg: 1.383 ± 0.014	1.478TyrSer: 1.478 ± 0.017	1.185TyrThr: 1.185 ± 0.014	1.41TyrVal: 1.41 ± 0.016	0.29TyrTrp: 0.29 ± 0.006	0.638TyrTyr: 0.638 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 14335 proteins (8189894 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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