Thiorhodospira sibirica ATCC 700588

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Thiorhodospira; Thiorhodospira sibirica

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2933 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4E8T0|G4E8T0_9GAMM UvrABC system protein C OS=Thiorhodospira sibirica ATCC 700588 OX=765914 GN=uvrC PE=3 SV=1
MM1 pKa = 7.14LTIDD5 pKa = 4.4PGLVCWLIEE14 pKa = 4.46RR15 pKa = 11.84IQEE18 pKa = 3.53FHH20 pKa = 6.46AQEE23 pKa = 4.06DD24 pKa = 4.5VAIHH28 pKa = 6.04EE29 pKa = 4.64EE30 pKa = 4.33PEE32 pKa = 4.09IPHH35 pKa = 7.23DD36 pKa = 3.91DD37 pKa = 3.27WGLAGMASVAEE48 pKa = 4.3YY49 pKa = 10.91ADD51 pKa = 3.9DD52 pKa = 4.3PAYY55 pKa = 10.63QEE57 pKa = 3.93IKK59 pKa = 11.1SNIDD63 pKa = 3.15EE64 pKa = 5.06LEE66 pKa = 4.48PEE68 pKa = 4.14QQVCLVALMWMGRR81 pKa = 11.84GDD83 pKa = 3.76FDD85 pKa = 5.66DD86 pKa = 6.0NEE88 pKa = 4.15WQLAFAEE95 pKa = 4.99AKK97 pKa = 10.65SNWTSHH103 pKa = 3.99TAEE106 pKa = 4.24YY107 pKa = 10.48LIATPLVSDD116 pKa = 4.12YY117 pKa = 11.0LAEE120 pKa = 4.44GLSMMGYY127 pKa = 9.65SCDD130 pKa = 5.33DD131 pKa = 3.31EE132 pKa = 5.02DD133 pKa = 4.49

Molecular weight: 15.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4E913|G4E913_9GAMM Adenine specific DNA methyltransferase (Fragment) OS=Thiorhodospira sibirica ATCC 700588 OX=765914 GN=ThisiDRAFT_2792 PE=4 SV=1
MM1 pKa = 6.96TKK3 pKa = 9.87EE4 pKa = 4.66HH5 pKa = 6.25ITMSHH10 pKa = 6.3KK11 pKa = 10.74EE12 pKa = 3.62IDD14 pKa = 3.93RR15 pKa = 11.84LEE17 pKa = 4.47MIQAVANKK25 pKa = 9.68HH26 pKa = 5.35LRR28 pKa = 11.84QAEE31 pKa = 3.9AAQRR35 pKa = 11.84LGLSVRR41 pKa = 11.84QVKK44 pKa = 10.25RR45 pKa = 11.84LVRR48 pKa = 11.84RR49 pKa = 11.84YY50 pKa = 9.34RR51 pKa = 11.84EE52 pKa = 3.9HH53 pKa = 6.84GATGLRR59 pKa = 11.84SGHH62 pKa = 6.46RR63 pKa = 11.84GRR65 pKa = 11.84RR66 pKa = 11.84PNNAIAEE73 pKa = 4.53TVRR76 pKa = 11.84QEE78 pKa = 3.75VLALVKK84 pKa = 8.87THH86 pKa = 5.74YY87 pKa = 9.91TDD89 pKa = 5.04FGPTLACEE97 pKa = 4.36KK98 pKa = 9.91LAEE101 pKa = 4.18RR102 pKa = 11.84HH103 pKa = 4.8GHH105 pKa = 5.44HH106 pKa = 6.84LSVEE110 pKa = 4.34TLRR113 pKa = 11.84QWMVADD119 pKa = 4.87GLWQPKK125 pKa = 7.08QRR127 pKa = 11.84KK128 pKa = 5.46QARR131 pKa = 11.84IHH133 pKa = 4.62QRR135 pKa = 11.84RR136 pKa = 11.84PRR138 pKa = 11.84RR139 pKa = 11.84PCLGEE144 pKa = 4.74LIQIDD149 pKa = 4.8GSPHH153 pKa = 6.22DD154 pKa = 3.8WFEE157 pKa = 4.06VRR159 pKa = 11.84GPRR162 pKa = 11.84CTLMVFIDD170 pKa = 4.27DD171 pKa = 3.55ATGRR175 pKa = 11.84LMALRR180 pKa = 11.84FVPAEE185 pKa = 3.83TTQAYY190 pKa = 8.75MEE192 pKa = 4.44TLQQYY197 pKa = 10.36LDD199 pKa = 3.33QHH201 pKa = 5.98GRR203 pKa = 11.84PVALYY208 pKa = 9.85SDD210 pKa = 3.34KK211 pKa = 11.26HH212 pKa = 7.26SIFRR216 pKa = 11.84VNHH219 pKa = 6.38PEE221 pKa = 3.61HH222 pKa = 7.23DD223 pKa = 4.05GEE225 pKa = 4.18LTQFSRR231 pKa = 11.84ALKK234 pKa = 9.01TLDD237 pKa = 3.08IAAIHH242 pKa = 6.74ANTPQAKK249 pKa = 9.79GRR251 pKa = 11.84VEE253 pKa = 3.95RR254 pKa = 11.84ANQTLQDD261 pKa = 3.78RR262 pKa = 11.84LVKK265 pKa = 9.89EE266 pKa = 3.66LRR268 pKa = 11.84LRR270 pKa = 11.84EE271 pKa = 3.95ISDD274 pKa = 3.05IDD276 pKa = 3.47AANAFLPTFMADD288 pKa = 3.3FNARR292 pKa = 11.84FAVEE296 pKa = 4.06PQSPPGLPTDD306 pKa = 4.29RR307 pKa = 11.84CCTAPRR313 pKa = 11.84SRR315 pKa = 11.84PSSFACTIPVSSPRR329 pKa = 11.84TFASSSRR336 pKa = 11.84TGISASGTGARR347 pKa = 11.84ATACGVPPSPSARR360 pKa = 11.84PSTAPSPSCTRR371 pKa = 11.84GTSCLTVCWLRR382 pKa = 11.84ANRR385 pKa = 11.84PHH387 pKa = 6.66RR388 pKa = 11.84WMMKK392 pKa = 9.76KK393 pKa = 10.47VSTTASTRR401 pKa = 11.84PRR403 pKa = 11.84QNRR406 pKa = 11.84PPSQPTNRR414 pKa = 11.84RR415 pKa = 11.84RR416 pKa = 11.84TTLGEE421 pKa = 4.0VGATSLRR428 pKa = 11.84HH429 pKa = 4.66QPKK432 pKa = 8.6HH433 pKa = 6.35HH434 pKa = 5.98NHH436 pKa = 6.24AFYY439 pKa = 10.8GGHH442 pKa = 6.29FCFAQKK448 pKa = 9.38GTFLNWVDD456 pKa = 3.81SRR458 pKa = 11.84NGSPVV463 pKa = 2.73

Molecular weight: 52.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2933

0

2933

923034

30

2404

314.7

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.825 ± 0.055

1.085 ± 0.017

5.142 ± 0.035

5.877 ± 0.048

3.472 ± 0.031

7.062 ± 0.043

2.952 ± 0.027

5.342 ± 0.035

3.097 ± 0.041

11.671 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.02

2.834 ± 0.036

5.096 ± 0.038

5.212 ± 0.042

6.956 ± 0.045

5.293 ± 0.034

5.128 ± 0.036

6.636 ± 0.037

1.333 ± 0.021

2.625 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski