Amino acid dipepetide frequency for Methylobacterium sp. 17Sr1-43

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.192AlaAla: 23.192 ± 0.217	1.429AlaCys: 1.429 ± 0.03	7.379AlaAsp: 7.379 ± 0.076	9.749AlaGlu: 9.749 ± 0.099	4.636AlaPhe: 4.636 ± 0.064	13.257AlaGly: 13.257 ± 0.121	2.529AlaHis: 2.529 ± 0.044	5.609AlaIle: 5.609 ± 0.061	3.395AlaLys: 3.395 ± 0.065	16.687AlaLeu: 16.687 ± 0.135	3.362AlaMet: 3.362 ± 0.052	2.45AlaAsn: 2.45 ± 0.048	7.532AlaPro: 7.532 ± 0.099	4.154AlaGln: 4.154 ± 0.056	12.541AlaArg: 12.541 ± 0.121	6.4AlaSer: 6.4 ± 0.063	6.345AlaThr: 6.345 ± 0.073	10.709AlaVal: 10.709 ± 0.088	1.809AlaTrp: 1.809 ± 0.035	2.857AlaTyr: 2.857 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.143CysAla: 1.143 ± 0.028	0.109CysCys: 0.109 ± 0.009	0.476CysAsp: 0.476 ± 0.016	0.413CysGlu: 0.413 ± 0.016	0.277CysPhe: 0.277 ± 0.015	0.948CysGly: 0.948 ± 0.029	0.201CysHis: 0.201 ± 0.013	0.326CysIle: 0.326 ± 0.015	0.136CysLys: 0.136 ± 0.01	0.957CysLeu: 0.957 ± 0.031	0.144CysMet: 0.144 ± 0.01	0.178CysAsn: 0.178 ± 0.01	0.505CysPro: 0.505 ± 0.016	0.186CysGln: 0.186 ± 0.011	0.773CysArg: 0.773 ± 0.024	0.358CysSer: 0.358 ± 0.014	0.438CysThr: 0.438 ± 0.022	0.627CysVal: 0.627 ± 0.022	0.088CysTrp: 0.088 ± 0.008	0.161CysTyr: 0.161 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.927AspAla: 7.927 ± 0.076	0.406AspCys: 0.406 ± 0.018	2.674AspAsp: 2.674 ± 0.049	3.089AspGlu: 3.089 ± 0.053	1.859AspPhe: 1.859 ± 0.036	5.446AspGly: 5.446 ± 0.061	1.105AspHis: 1.105 ± 0.03	2.159AspIle: 2.159 ± 0.037	1.154AspLys: 1.154 ± 0.033	6.703AspLeu: 6.703 ± 0.075	0.866AspMet: 0.866 ± 0.024	0.887AspAsn: 0.887 ± 0.025	4.183AspPro: 4.183 ± 0.065	1.391AspGln: 1.391 ± 0.033	4.83AspArg: 4.83 ± 0.055	1.642AspSer: 1.642 ± 0.035	2.391AspThr: 2.391 ± 0.041	3.874AspVal: 3.874 ± 0.048	0.826AspTrp: 0.826 ± 0.024	1.173AspTyr: 1.173 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	10.02GluAla: 10.02 ± 0.097	0.284GluCys: 0.284 ± 0.014	2.52GluAsp: 2.52 ± 0.047	2.9GluGlu: 2.9 ± 0.051	1.266GluPhe: 1.266 ± 0.033	4.759GluGly: 4.759 ± 0.067	1.163GluHis: 1.163 ± 0.031	3.13GluIle: 3.13 ± 0.056	1.462GluLys: 1.462 ± 0.039	4.428GluLeu: 4.428 ± 0.053	1.261GluMet: 1.261 ± 0.032	1.135GluAsn: 1.135 ± 0.029	3.523GluPro: 3.523 ± 0.056	1.685GluGln: 1.685 ± 0.037	6.363GluArg: 6.363 ± 0.084	2.304GluSer: 2.304 ± 0.039	3.646GluThr: 3.646 ± 0.054	3.753GluVal: 3.753 ± 0.059	0.519GluTrp: 0.519 ± 0.018	0.792GluTyr: 0.792 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.578PheAla: 4.578 ± 0.055	0.373PheCys: 0.373 ± 0.017	2.179PheAsp: 2.179 ± 0.039	1.955PheGlu: 1.955 ± 0.039	1.1PhePhe: 1.1 ± 0.029	3.574PheGly: 3.574 ± 0.054	0.619PheHis: 0.619 ± 0.019	1.124PheIle: 1.124 ± 0.027	0.693PheLys: 0.693 ± 0.021	3.182PheLeu: 3.182 ± 0.052	0.586PheMet: 0.586 ± 0.022	0.738PheAsn: 0.738 ± 0.026	1.511PhePro: 1.511 ± 0.039	0.812PheGln: 0.812 ± 0.024	2.503PheArg: 2.503 ± 0.041	1.761PheSer: 1.761 ± 0.034	1.805PheThr: 1.805 ± 0.039	2.695PheVal: 2.695 ± 0.047	0.477PheTrp: 0.477 ± 0.018	0.76PheTyr: 0.76 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	11.544GlyAla: 11.544 ± 0.104	0.871GlyCys: 0.871 ± 0.029	4.147GlyAsp: 4.147 ± 0.061	4.886GlyGlu: 4.886 ± 0.068	3.54GlyPhe: 3.54 ± 0.052	7.891GlyGly: 7.891 ± 0.086	1.949GlyHis: 1.949 ± 0.042	4.205GlyIle: 4.205 ± 0.068	2.294GlyLys: 2.294 ± 0.041	10.895GlyLeu: 10.895 ± 0.106	1.922GlyMet: 1.922 ± 0.034	1.762GlyAsn: 1.762 ± 0.038	4.865GlyPro: 4.865 ± 0.061	2.91GlyGln: 2.91 ± 0.048	8.323GlyArg: 8.323 ± 0.081	4.78GlySer: 4.78 ± 0.06	5.242GlyThr: 5.242 ± 0.075	6.097GlyVal: 6.097 ± 0.056	1.387GlyTrp: 1.387 ± 0.031	2.286GlyTyr: 2.286 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.704HisAla: 2.704 ± 0.048	0.197HisCys: 0.197 ± 0.011	1.19HisAsp: 1.19 ± 0.029	0.955HisGlu: 0.955 ± 0.025	0.68HisPhe: 0.68 ± 0.025	1.951HisGly: 1.951 ± 0.038	0.543HisHis: 0.543 ± 0.023	0.691HisIle: 0.691 ± 0.023	0.33HisLys: 0.33 ± 0.015	2.124HisLeu: 2.124 ± 0.041	0.355HisMet: 0.355 ± 0.017	0.375HisAsn: 0.375 ± 0.016	1.371HisPro: 1.371 ± 0.031	0.444HisGln: 0.444 ± 0.016	1.686HisArg: 1.686 ± 0.035	0.65HisSer: 0.65 ± 0.02	0.735HisThr: 0.735 ± 0.026	1.415HisVal: 1.415 ± 0.031	0.295HisTrp: 0.295 ± 0.015	0.464HisTyr: 0.464 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.378IleAla: 6.378 ± 0.074	0.403IleCys: 0.403 ± 0.016	2.911IleAsp: 2.911 ± 0.041	2.976IleGlu: 2.976 ± 0.049	1.176IlePhe: 1.176 ± 0.025	4.443IleGly: 4.443 ± 0.06	0.73IleHis: 0.73 ± 0.022	1.277IleIle: 1.277 ± 0.029	0.932IleLys: 0.932 ± 0.029	4.069IleLeu: 4.069 ± 0.056	0.595IleMet: 0.595 ± 0.025	0.976IleAsn: 0.976 ± 0.026	2.053IlePro: 2.053 ± 0.038	1.069IleGln: 1.069 ± 0.027	3.224IleArg: 3.224 ± 0.047	1.867IleSer: 1.867 ± 0.033	2.096IleThr: 2.096 ± 0.042	3.615IleVal: 3.615 ± 0.058	0.453IleTrp: 0.453 ± 0.017	0.816IleTyr: 0.816 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	3.482LysAla: 3.482 ± 0.062	0.115LysCys: 0.115 ± 0.008	1.242LysAsp: 1.242 ± 0.033	1.062LysGlu: 1.062 ± 0.031	0.596LysPhe: 0.596 ± 0.021	2.052LysGly: 2.052 ± 0.045	0.39LysHis: 0.39 ± 0.018	1.126LysIle: 1.126 ± 0.028	0.71LysLys: 0.71 ± 0.027	2.392LysLeu: 2.392 ± 0.046	0.442LysMet: 0.442 ± 0.017	0.564LysAsn: 0.564 ± 0.021	1.87LysPro: 1.87 ± 0.043	0.682LysGln: 0.682 ± 0.021	1.926LysArg: 1.926 ± 0.041	1.172LysSer: 1.172 ± 0.031	1.391LysThr: 1.391 ± 0.035	1.85LysVal: 1.85 ± 0.045	0.219LysTrp: 0.219 ± 0.012	0.383LysTyr: 0.383 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	17.136LeuAla: 17.136 ± 0.14	0.999LeuCys: 0.999 ± 0.025	6.737LeuAsp: 6.737 ± 0.068	4.796LeuGlu: 4.796 ± 0.063	3.329LeuPhe: 3.329 ± 0.055	9.35LeuGly: 9.35 ± 0.11	1.887LeuHis: 1.887 ± 0.038	4.284LeuIle: 4.284 ± 0.063	2.891LeuLys: 2.891 ± 0.049	9.561LeuLeu: 9.561 ± 0.108	1.986LeuMet: 1.986 ± 0.042	2.187LeuAsn: 2.187 ± 0.033	6.296LeuPro: 6.296 ± 0.068	2.449LeuGln: 2.449 ± 0.038	8.395LeuArg: 8.395 ± 0.083	5.647LeuSer: 5.647 ± 0.064	5.769LeuThr: 5.769 ± 0.065	8.528LeuVal: 8.528 ± 0.077	1.167LeuTrp: 1.167 ± 0.03	2.022LeuTyr: 2.022 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.494MetAla: 2.494 ± 0.043	0.126MetCys: 0.126 ± 0.01	0.853MetAsp: 0.853 ± 0.025	0.915MetGlu: 0.915 ± 0.025	0.488MetPhe: 0.488 ± 0.02	1.504MetGly: 1.504 ± 0.037	0.336MetHis: 0.336 ± 0.014	0.985MetIle: 0.985 ± 0.028	0.602MetLys: 0.602 ± 0.019	2.079MetLeu: 2.079 ± 0.036	0.495MetMet: 0.495 ± 0.021	0.589MetAsn: 0.589 ± 0.02	1.504MetPro: 1.504 ± 0.031	0.643MetGln: 0.643 ± 0.02	1.952MetArg: 1.952 ± 0.032	1.368MetSer: 1.368 ± 0.032	1.381MetThr: 1.381 ± 0.035	1.287MetVal: 1.287 ± 0.03	0.136MetTrp: 0.136 ± 0.01	0.205MetTyr: 0.205 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.816AsnAla: 2.816 ± 0.047	0.182AsnCys: 0.182 ± 0.012	1.05AsnAsp: 1.05 ± 0.023	0.95AsnGlu: 0.95 ± 0.029	0.682AsnPhe: 0.682 ± 0.021	1.898AsnGly: 1.898 ± 0.042	0.414AsnHis: 0.414 ± 0.017	0.862AsnIle: 0.862 ± 0.022	0.437AsnLys: 0.437 ± 0.021	2.293AsnLeu: 2.293 ± 0.038	0.379AsnMet: 0.379 ± 0.016	0.515AsnAsn: 0.515 ± 0.021	1.645AsnPro: 1.645 ± 0.039	0.567AsnGln: 0.567 ± 0.021	1.72AsnArg: 1.72 ± 0.035	0.782AsnSer: 0.782 ± 0.024	1.031AsnThr: 1.031 ± 0.026	1.584AsnVal: 1.584 ± 0.033	0.285AsnTrp: 0.285 ± 0.016	0.459AsnTyr: 0.459 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	8.633ProAla: 8.633 ± 0.104	0.377ProCys: 0.377 ± 0.017	4.278ProAsp: 4.278 ± 0.055	4.592ProGlu: 4.592 ± 0.056	2.027ProPhe: 2.027 ± 0.044	5.955ProGly: 5.955 ± 0.073	1.099ProHis: 1.099 ± 0.028	1.997ProIle: 1.997 ± 0.036	1.489ProLys: 1.489 ± 0.039	5.192ProLeu: 5.192 ± 0.069	1.13ProMet: 1.13 ± 0.026	1.257ProAsn: 1.257 ± 0.029	3.399ProPro: 3.399 ± 0.061	1.59ProGln: 1.59 ± 0.038	4.071ProArg: 4.071 ± 0.06	2.635ProSer: 2.635 ± 0.044	2.601ProThr: 2.601 ± 0.043	4.886ProVal: 4.886 ± 0.057	0.746ProTrp: 0.746 ± 0.026	1.134ProTyr: 1.134 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.487GlnAla: 4.487 ± 0.059	0.17GlnCys: 0.17 ± 0.012	1.4GlnAsp: 1.4 ± 0.033	1.295GlnGlu: 1.295 ± 0.034	0.809GlnPhe: 0.809 ± 0.022	2.58GlnGly: 2.58 ± 0.039	0.508GlnHis: 0.508 ± 0.018	1.508GlnIle: 1.508 ± 0.034	0.741GlnLys: 0.741 ± 0.02	2.25GlnLeu: 2.25 ± 0.043	0.59GlnMet: 0.59 ± 0.018	0.692GlnAsn: 0.692 ± 0.024	1.679GlnPro: 1.679 ± 0.035	0.92GlnGln: 0.92 ± 0.03	2.233GlnArg: 2.233 ± 0.043	1.377GlnSer: 1.377 ± 0.029	1.499GlnThr: 1.499 ± 0.037	2.257GlnVal: 2.257 ± 0.039	0.256GlnTrp: 0.256 ± 0.013	0.484GlnTyr: 0.484 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	11.815ArgAla: 11.815 ± 0.101	0.567ArgCys: 0.567 ± 0.022	4.794ArgAsp: 4.794 ± 0.062	4.699ArgGlu: 4.699 ± 0.067	3.191ArgPhe: 3.191 ± 0.05	6.31ArgGly: 6.31 ± 0.068	1.862ArgHis: 1.862 ± 0.039	4.134ArgIle: 4.134 ± 0.052	1.808ArgLys: 1.808 ± 0.044	9.709ArgLeu: 9.709 ± 0.089	1.839ArgMet: 1.839 ± 0.038	1.703ArgAsn: 1.703 ± 0.035	5.068ArgPro: 5.068 ± 0.075	2.555ArgGln: 2.555 ± 0.042	7.741ArgArg: 7.741 ± 0.098	3.957ArgSer: 3.957 ± 0.055	4.217ArgThr: 4.217 ± 0.054	6.002ArgVal: 6.002 ± 0.075	1.071ArgTrp: 1.071 ± 0.028	1.79ArgTyr: 1.79 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	6.11SerAla: 6.11 ± 0.066	0.385SerCys: 0.385 ± 0.017	2.481SerAsp: 2.481 ± 0.039	2.679SerGlu: 2.679 ± 0.043	1.841SerPhe: 1.841 ± 0.039	5.152SerGly: 5.152 ± 0.063	0.877SerHis: 0.877 ± 0.027	1.879SerIle: 1.879 ± 0.04	1.005SerLys: 1.005 ± 0.026	5.134SerLeu: 5.134 ± 0.056	0.904SerMet: 0.904 ± 0.023	0.953SerAsn: 0.953 ± 0.024	2.679SerPro: 2.679 ± 0.047	1.27SerGln: 1.27 ± 0.026	3.483SerArg: 3.483 ± 0.045	2.134SerSer: 2.134 ± 0.041	2.288SerThr: 2.288 ± 0.041	3.627SerVal: 3.627 ± 0.043	0.584SerTrp: 0.584 ± 0.019	1.092SerTyr: 1.092 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	6.566ThrAla: 6.566 ± 0.084	0.454ThrCys: 0.454 ± 0.017	2.694ThrAsp: 2.694 ± 0.037	2.592ThrGlu: 2.592 ± 0.042	1.84ThrPhe: 1.84 ± 0.039	5.462ThrGly: 5.462 ± 0.066	0.917ThrHis: 0.917 ± 0.028	2.348ThrIle: 2.348 ± 0.041	1.168ThrLys: 1.168 ± 0.033	6.013ThrLeu: 6.013 ± 0.063	0.906ThrMet: 0.906 ± 0.023	1.083ThrAsn: 1.083 ± 0.028	3.215ThrPro: 3.215 ± 0.049	1.345ThrGln: 1.345 ± 0.031	3.75ThrArg: 3.75 ± 0.059	2.241ThrSer: 2.241 ± 0.041	2.541ThrThr: 2.541 ± 0.048	4.666ThrVal: 4.666 ± 0.06	0.672ThrTrp: 0.672 ± 0.022	1.164ThrTyr: 1.164 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	10.956ValAla: 10.956 ± 0.1	0.666ValCys: 0.666 ± 0.021	3.75ValAsp: 3.75 ± 0.051	4.775ValGlu: 4.775 ± 0.062	2.496ValPhe: 2.496 ± 0.043	6.185ValGly: 6.185 ± 0.06	1.351ValHis: 1.351 ± 0.029	3.212ValIle: 3.212 ± 0.048	1.723ValLys: 1.723 ± 0.044	8.047ValLeu: 8.047 ± 0.075	1.552ValMet: 1.552 ± 0.035	1.684ValAsn: 1.684 ± 0.035	4.524ValPro: 4.524 ± 0.059	2.094ValGln: 2.094 ± 0.04	6.055ValArg: 6.055 ± 0.08	3.917ValSer: 3.917 ± 0.049	4.513ValThr: 4.513 ± 0.051	6.417ValVal: 6.417 ± 0.075	0.85ValTrp: 0.85 ± 0.027	1.356ValTyr: 1.356 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.248TrpAla: 1.248 ± 0.026	0.13TrpCys: 0.13 ± 0.009	0.579TrpAsp: 0.579 ± 0.019	0.492TrpGlu: 0.492 ± 0.018	0.497TrpPhe: 0.497 ± 0.019	0.86TrpGly: 0.86 ± 0.024	0.285TrpHis: 0.285 ± 0.014	0.581TrpIle: 0.581 ± 0.02	0.288TrpLys: 0.288 ± 0.014	1.571TrpLeu: 1.571 ± 0.033	0.294TrpMet: 0.294 ± 0.015	0.351TrpAsn: 0.351 ± 0.019	0.664TrpPro: 0.664 ± 0.02	0.454TrpGln: 0.454 ± 0.019	1.304TrpArg: 1.304 ± 0.034	0.778TrpSer: 0.778 ± 0.023	0.747TrpThr: 0.747 ± 0.022	0.729TrpVal: 0.729 ± 0.024	0.212TrpTrp: 0.212 ± 0.012	0.267TrpTyr: 0.267 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.793TyrAla: 2.793 ± 0.047	0.202TyrCys: 0.202 ± 0.011	1.252TyrAsp: 1.252 ± 0.029	1.138TyrGlu: 1.138 ± 0.027	0.695TyrPhe: 0.695 ± 0.021	2.222TyrGly: 2.222 ± 0.05	0.387TyrHis: 0.387 ± 0.015	0.622TyrIle: 0.622 ± 0.021	0.405TyrLys: 0.405 ± 0.017	2.13TyrLeu: 2.13 ± 0.041	0.337TyrMet: 0.337 ± 0.014	0.472TyrAsn: 0.472 ± 0.022	1.001TyrPro: 1.001 ± 0.026	0.514TyrGln: 0.514 ± 0.019	1.961TyrArg: 1.961 ± 0.035	0.788TyrSer: 0.788 ± 0.023	0.959TyrThr: 0.959 ± 0.026	1.506TyrVal: 1.506 ± 0.04	0.282TyrTrp: 0.282 ± 0.012	0.459TyrTyr: 0.459 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4920 proteins (1526070 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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