Propioniciclava sp. HDW11

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Brevilactibacter

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7Y5R0|A0A6G7Y5R0_9ACTN DUF559 domain-containing protein OS=Propioniciclava sp. HDW11 OX=2714937 GN=G7070_06390 PE=4 SV=1
MM1 pKa = 7.81PNLAPYY7 pKa = 10.01IAFPGNAKK15 pKa = 10.3DD16 pKa = 3.81AFEE19 pKa = 4.79HH20 pKa = 5.87YY21 pKa = 10.34RR22 pKa = 11.84DD23 pKa = 3.77VFGGDD28 pKa = 3.32LQLFTYY34 pKa = 11.1ADD36 pKa = 3.95MPMEE40 pKa = 4.94GAPSTLDD47 pKa = 3.6PASVAHH53 pKa = 7.14ASLTLPGGTIVGGDD67 pKa = 3.55AMPGEE72 pKa = 5.38DD73 pKa = 3.74YY74 pKa = 10.75PIRR77 pKa = 11.84GSAYY81 pKa = 8.74SLMYY85 pKa = 10.73SLEE88 pKa = 4.69DD89 pKa = 3.65PDD91 pKa = 3.43EE92 pKa = 4.14ARR94 pKa = 11.84RR95 pKa = 11.84LIALLVDD102 pKa = 4.13AGGSVGMPFEE112 pKa = 4.18PAPWGAYY119 pKa = 8.66YY120 pKa = 10.64GQVFDD125 pKa = 4.72RR126 pKa = 11.84FGVMWAFDD134 pKa = 3.59VDD136 pKa = 4.22AEE138 pKa = 4.54EE139 pKa = 4.45PTQAA143 pKa = 4.49

Molecular weight: 15.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7Y644|A0A6G7Y644_9ACTN Uncharacterized protein OS=Propioniciclava sp. HDW11 OX=2714937 GN=G7070_08175 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.18GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.2GRR40 pKa = 11.84VRR42 pKa = 11.84LAGG45 pKa = 3.53

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3100

0

3100

963778

29

1829

310.9

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.126 ± 0.058

0.668 ± 0.012

6.172 ± 0.04

5.233 ± 0.038

3.015 ± 0.029

9.172 ± 0.039

2.069 ± 0.02

3.85 ± 0.029

1.963 ± 0.03

10.452 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.016

1.87 ± 0.021

5.917 ± 0.034

2.757 ± 0.022

7.46 ± 0.047

4.981 ± 0.028

6.019 ± 0.031

8.752 ± 0.041

1.672 ± 0.021

1.907 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski