Mesorhizobium metallidurans STM 2683

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; Mesorhizobium metallidurans

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6696 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5F3Q4|M5F3Q4_9RHIZ Cytochrome P-450 hydroxylase OS=Mesorhizobium metallidurans STM 2683 OX=1297569 GN=MESS2_320017 PE=3 SV=1
MM1 pKa = 7.38AASSVALVWYY11 pKa = 9.21LVIAGPQGGMVVLPSTFDD29 pKa = 3.21TRR31 pKa = 11.84EE32 pKa = 3.59QCTNAVTEE40 pKa = 4.33YY41 pKa = 10.45QKK43 pKa = 10.87QPTPTGWSVQCVPSASPFTDD63 pKa = 2.93EE64 pKa = 5.19GDD66 pKa = 3.7AEE68 pKa = 4.36EE69 pKa = 5.35PSAQQ73 pKa = 3.51

Molecular weight: 7.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5F3H8|M5F3H8_9RHIZ Polar amino acid ABC transporter inner membrane subunit OS=Mesorhizobium metallidurans STM 2683 OX=1297569 GN=MESS2_310021 PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PLLGLSHH9 pKa = 7.55LSHH12 pKa = 6.37QKK14 pKa = 9.63WFTPTTGSTSSHH26 pKa = 5.73TGIIAARR33 pKa = 11.84PKK35 pKa = 10.58KK36 pKa = 9.32LTLAIRR42 pKa = 11.84RR43 pKa = 11.84FLLMGLHH50 pKa = 6.5AKK52 pKa = 9.96LRR54 pKa = 11.84AKK56 pKa = 10.46RR57 pKa = 11.84SQKK60 pKa = 10.09PP61 pKa = 3.07

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6696

0

6696

1837213

20

2862

274.4

29.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.308 ± 0.038

0.887 ± 0.011

5.681 ± 0.025

5.521 ± 0.031

3.851 ± 0.02

8.56 ± 0.035

2.045 ± 0.015

5.436 ± 0.021

3.718 ± 0.029

9.864 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.013

2.694 ± 0.016

5.068 ± 0.023

3.014 ± 0.019

7.093 ± 0.031

5.641 ± 0.022

5.174 ± 0.021

7.366 ± 0.025

1.339 ± 0.013

2.21 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski