Nitrosomonas cryotolerans ATCC 49181 
Average proteome isoelectric point is 6.65 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2414 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A1N6G367|A0A1N6G367_9PROT Transposase  IS605 OrfB family  central region OS=Nitrosomonas cryotolerans ATCC 49181 OX=1131553 GN=SAMN02743940_0528 PE=3 SV=1MM1 pKa = 7.2  ISYY4 pKa = 10.33  RR5 pKa = 11.84  RR6 pKa = 11.84  FKK8 pKa = 10.82  SQISITLLVTALSWLVCNLAVAAPPTNDD36 pKa = 2.69  TFTNSTSVTVGFNEE50 pKa = 4.34  VLDD53 pKa = 4.08  TTEE56 pKa = 4.19  ATTDD60 pKa = 3.38  SDD62 pKa = 4.42  DD63 pKa = 3.72  AQLNMSCGAPASDD76 pKa = 3.03  ASVWYY81 pKa = 10.33  SFNATSDD88 pKa = 3.61  TNVVVDD94 pKa = 4.42  VSQSNYY100 pKa = 9.3  SAGVLVSEE108 pKa = 5.46  GSQDD112 pKa = 3.49  NLQTIACGPGAVPFFATAGTTYY134 pKa = 10.75  YY135 pKa = 11.11  VLAIDD140 pKa = 4.71  DD141 pKa = 3.96  QLDD144 pKa = 3.58  GGGNGGLLSISFNEE158 pKa = 4.24  VAPTTLDD165 pKa = 3.6  DD166 pKa = 3.95  FTVNPVGIVNTRR178 pKa = 11.84  TGVATISGTYY188 pKa = 7.79  TCNNGDD194 pKa = 3.9  FLGLFADD201 pKa = 4.8  ASQDD205 pKa = 3.25  VGRR208 pKa = 11.84  IATIRR213 pKa = 11.84  GDD215 pKa = 3.85  FFFFDD220 pKa = 4.1  SGTCNGTSHH229 pKa = 7.27  PWSGNVVPQNGKK241 pKa = 9.31  FAGGKK246 pKa = 9.99  AMTMTFAFTCGPFQCADD263 pKa = 3.49  GYY265 pKa = 10.59  IEE267 pKa = 5.14  QKK269 pKa = 9.68  IQLRR273 pKa = 11.84  GGKK276 pKa = 9.2  KK277 pKa = 9.65  
 29.06 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.78 
IPC2_protein 4.012 
IPC_protein 4.012 
Toseland    3.783 
ProMoST     4.19 
Dawson      4.024 
Bjellqvist  4.177 
Wikipedia   3.986 
Rodwell     3.834 
Grimsley    3.681 
Solomon     4.012 
Lehninger   3.973 
Nozaki      4.139 
DTASelect   4.431 
Thurlkill   3.846 
EMBOSS      3.999 
Sillero     4.139 
Patrickios  1.138 
IPC_peptide 4.012 
IPC2_peptide  4.113 
IPC2.peptide.svr19  4.006 
 Protein with the highest isoelectric point: 
>tr|A0A1N6GXW4|A0A1N6GXW4_9PROT 3-phosphoshikimate 1-carboxyvinyltransferase OS=Nitrosomonas cryotolerans ATCC 49181 OX=1131553 GN=aroA PE=3 SV=1MM1 pKa = 7.35  KK2 pKa = 9.36  RR3 pKa = 11.84  TYY5 pKa = 9.6  QPSVIRR11 pKa = 11.84  RR12 pKa = 11.84  RR13 pKa = 11.84  RR14 pKa = 11.84  THH16 pKa = 5.9  GFLVRR21 pKa = 11.84  MKK23 pKa = 9.48  TRR25 pKa = 11.84  GGAAVIRR32 pKa = 11.84  ARR34 pKa = 11.84  RR35 pKa = 11.84  AKK37 pKa = 9.98  GRR39 pKa = 11.84  VRR41 pKa = 11.84  LGVV44 pKa = 3.2  
 5.12 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.445 
IPC2_protein 11.155 
IPC_protein 12.544 
Toseland    12.705 
ProMoST     13.203 
Dawson      12.705 
Bjellqvist  12.705 
Wikipedia   13.188 
Rodwell     12.31 
Grimsley    12.749 
Solomon     13.203 
Lehninger   13.1 
Nozaki      12.705 
DTASelect   12.705 
Thurlkill   12.705 
EMBOSS      13.203 
Sillero     12.705 
Patrickios  12.047 
IPC_peptide 13.203 
IPC2_peptide  12.193 
IPC2.peptide.svr19  9.109 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2414 
0
2414 
781301
19
2921
323.7
36.09
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        8.658 ± 0.05
1.045 ± 0.016
5.141 ± 0.035
5.787 ± 0.038
4.058 ± 0.034
6.791 ± 0.049
2.577 ± 0.025
7.231 ± 0.043
4.968 ± 0.054
10.716 ± 0.057
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.557 ± 0.022
4.204 ± 0.04
4.176 ± 0.029
4.321 ± 0.038
5.527 ± 0.036
6.272 ± 0.035
5.392 ± 0.031
6.359 ± 0.037
1.281 ± 0.022
2.94 ± 0.027
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here