Nitrosomonas cryotolerans ATCC 49181

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; Nitrosomonas cryotolerans

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1N6G367|A0A1N6G367_9PROT Transposase IS605 OrfB family central region OS=Nitrosomonas cryotolerans ATCC 49181 OX=1131553 GN=SAMN02743940_0528 PE=3 SV=1
MM1 pKa = 7.2ISYY4 pKa = 10.33RR5 pKa = 11.84RR6 pKa = 11.84FKK8 pKa = 10.82SQISITLLVTALSWLVCNLAVAAPPTNDD36 pKa = 2.69TFTNSTSVTVGFNEE50 pKa = 4.34VLDD53 pKa = 4.08TTEE56 pKa = 4.19ATTDD60 pKa = 3.38SDD62 pKa = 4.42DD63 pKa = 3.72AQLNMSCGAPASDD76 pKa = 3.03ASVWYY81 pKa = 10.33SFNATSDD88 pKa = 3.61TNVVVDD94 pKa = 4.42VSQSNYY100 pKa = 9.3SAGVLVSEE108 pKa = 5.46GSQDD112 pKa = 3.49NLQTIACGPGAVPFFATAGTTYY134 pKa = 10.75YY135 pKa = 11.11VLAIDD140 pKa = 4.71DD141 pKa = 3.96QLDD144 pKa = 3.58GGGNGGLLSISFNEE158 pKa = 4.24VAPTTLDD165 pKa = 3.6DD166 pKa = 3.95FTVNPVGIVNTRR178 pKa = 11.84TGVATISGTYY188 pKa = 7.79TCNNGDD194 pKa = 3.9FLGLFADD201 pKa = 4.8ASQDD205 pKa = 3.25VGRR208 pKa = 11.84IATIRR213 pKa = 11.84GDD215 pKa = 3.85FFFFDD220 pKa = 4.1SGTCNGTSHH229 pKa = 7.27PWSGNVVPQNGKK241 pKa = 9.31FAGGKK246 pKa = 9.99AMTMTFAFTCGPFQCADD263 pKa = 3.49GYY265 pKa = 10.59IEE267 pKa = 5.14QKK269 pKa = 9.68IQLRR273 pKa = 11.84GGKK276 pKa = 9.2KK277 pKa = 9.65

Molecular weight:
29.06 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1N6GXW4|A0A1N6GXW4_9PROT 3-phosphoshikimate 1-carboxyvinyltransferase OS=Nitrosomonas cryotolerans ATCC 49181 OX=1131553 GN=aroA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.6QPSVIRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84THH16 pKa = 5.9GFLVRR21 pKa = 11.84MKK23 pKa = 9.48TRR25 pKa = 11.84GGAAVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84VRR41 pKa = 11.84LGVV44 pKa = 3.2

Molecular weight:
5.12 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2414

0

2414

781301

19

2921

323.7

36.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.658 ± 0.05

1.045 ± 0.016

5.141 ± 0.035

5.787 ± 0.038

4.058 ± 0.034

6.791 ± 0.049

2.577 ± 0.025

7.231 ± 0.043

4.968 ± 0.054

10.716 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.022

4.204 ± 0.04

4.176 ± 0.029

4.321 ± 0.038

5.527 ± 0.036

6.272 ± 0.035

5.392 ± 0.031

6.359 ± 0.037

1.281 ± 0.022

2.94 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski