Halomicrobium zhouii

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Halomicrobium

Average proteome isoelectric point is 4.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4091 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6K6R6|A0A1I6K6R6_9EURY Universal stress protein family protein OS=Halomicrobium zhouii OX=767519 GN=SAMN05216559_0234 PE=4 SV=1
MM1 pKa = 7.24TGVAEE6 pKa = 4.33VVDD9 pKa = 4.79GVTTTLLCGASLGGEE24 pKa = 3.79TRR26 pKa = 11.84EE27 pKa = 4.17CCEE30 pKa = 3.63ALLRR34 pKa = 11.84AGADD38 pKa = 3.56DD39 pKa = 3.73GRR41 pKa = 11.84EE42 pKa = 4.19VLWVTYY48 pKa = 7.29TRR50 pKa = 11.84PPADD54 pKa = 4.1CLASVPDD61 pKa = 4.03DD62 pKa = 3.78VSVRR66 pKa = 11.84GVLAVGDD73 pKa = 3.98APHH76 pKa = 7.37WEE78 pKa = 4.08TSIDD82 pKa = 3.82GVDD85 pKa = 3.68VEE87 pKa = 5.48VVATPGDD94 pKa = 3.54ITALGIKK101 pKa = 10.36LSRR104 pKa = 11.84FLSGGDD110 pKa = 4.14DD111 pKa = 3.99DD112 pKa = 5.39LTVCFDD118 pKa = 4.16SVTAMCQYY126 pKa = 10.62VEE128 pKa = 4.66PEE130 pKa = 3.69TAYY133 pKa = 10.75GFLHH137 pKa = 6.75TIAVQLYY144 pKa = 8.5AADD147 pKa = 3.79ATAHH151 pKa = 5.29FHH153 pKa = 6.84IDD155 pKa = 3.48PAAHH159 pKa = 7.02DD160 pKa = 4.31ASTVDD165 pKa = 5.43LFASLCDD172 pKa = 3.34AVVNVGDD179 pKa = 5.77DD180 pKa = 3.9GDD182 pKa = 4.87DD183 pKa = 3.88DD184 pKa = 4.28PAVRR188 pKa = 11.84TRR190 pKa = 11.84PVLDD194 pKa = 3.47

Molecular weight: 20.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6KZH9|A0A1I6KZH9_9EURY Uncharacterized protein OS=Halomicrobium zhouii OX=767519 GN=SAMN05216559_1664 PE=4 SV=1
MM1 pKa = 7.63LFPVRR6 pKa = 11.84FVVSPEE12 pKa = 3.34GSAAVLVAILAGVALGTASGLTPGLHH38 pKa = 6.86ANTFALGLAAVASSVPGPPTLVGATMLAAGTTHH71 pKa = 6.94TFLDD75 pKa = 4.09VVPALALGVPDD86 pKa = 4.19PAMAASALPGHH97 pKa = 6.7RR98 pKa = 11.84LVIAGRR104 pKa = 11.84GRR106 pKa = 11.84EE107 pKa = 4.26AIRR110 pKa = 11.84LSALGSGLAVVFAVPLAIPVTLAMQRR136 pKa = 11.84AYY138 pKa = 9.07PTIRR142 pKa = 11.84AHH144 pKa = 7.67LSLLLAGVAVLLVVTEE160 pKa = 4.37RR161 pKa = 11.84TPSGKK166 pKa = 10.32VGATLTLVASAALGFVTLDD185 pKa = 3.72LTPQAPLPVGGMLAPLFAGLFGAPVLIEE213 pKa = 4.04SLGGGGVPPQDD224 pKa = 3.95DD225 pKa = 3.51ATLAFSRR232 pKa = 11.84RR233 pKa = 11.84DD234 pKa = 3.48VGALALLGTGAGAIVGYY251 pKa = 10.2IPGVSAAIASAAALGVLSGSGPRR274 pKa = 11.84AFVVTTSGVNTATAIFALFALVALGSPRR302 pKa = 11.84TGVLVAVEE310 pKa = 4.22SAGIPIDD317 pKa = 4.35LPVLLASVGFAAVVAAVLVPVLGDD341 pKa = 4.53RR342 pKa = 11.84YY343 pKa = 10.57LDD345 pKa = 3.18WARR348 pKa = 11.84RR349 pKa = 11.84ANQNRR354 pKa = 11.84LAIGILSLLVALSFLFAGFVGIGAFGVATVVGSIPARR391 pKa = 11.84FGAKK395 pKa = 9.42RR396 pKa = 11.84ANCMGVLLGPLALSS410 pKa = 3.79

Molecular weight: 40.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4091

0

4091

1202276

30

2225

293.9

31.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.904 ± 0.047

0.684 ± 0.013

8.823 ± 0.052

8.313 ± 0.054

3.335 ± 0.025

8.701 ± 0.041

2.001 ± 0.019

3.599 ± 0.028

1.649 ± 0.023

8.756 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.703 ± 0.015

2.315 ± 0.025

4.637 ± 0.028

2.668 ± 0.023

6.426 ± 0.034

5.612 ± 0.032

6.446 ± 0.041

9.518 ± 0.044

1.227 ± 0.016

2.683 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski