Entomoplasma luminosum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae; Entomoplasma

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 866 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8NU13|A0A2K8NU13_9MOLU Riboflavin kinase OS=Entomoplasma luminosum OX=214888 GN=ribC PE=4 SV=1
MM1 pKa = 7.42NKK3 pKa = 9.63YY4 pKa = 9.67EE5 pKa = 4.13KK6 pKa = 10.28EE7 pKa = 3.83KK8 pKa = 10.67LYY10 pKa = 11.09SILCDD15 pKa = 3.5ANKK18 pKa = 10.6AIIDD22 pKa = 3.99FKK24 pKa = 11.67DD25 pKa = 3.51EE26 pKa = 4.41LDD28 pKa = 4.66EE29 pKa = 4.07MTQDD33 pKa = 4.03DD34 pKa = 5.31EE35 pKa = 4.51EE36 pKa = 4.26FTYY39 pKa = 10.56CRR41 pKa = 11.84RR42 pKa = 11.84DD43 pKa = 3.46CGDD46 pKa = 3.38EE47 pKa = 4.09EE48 pKa = 5.0ACVGHH53 pKa = 7.36DD54 pKa = 4.38EE55 pKa = 4.4EE56 pKa = 4.99FCEE59 pKa = 4.48AFCGLEE65 pKa = 4.18DD66 pKa = 5.47CDD68 pKa = 3.7MHH70 pKa = 8.33QDD72 pKa = 4.7DD73 pKa = 4.9EE74 pKa = 6.11GEE76 pKa = 4.43LEE78 pKa = 4.18NEE80 pKa = 4.16

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8NSW9|A0A2K8NSW9_9MOLU Single-stranded DNA-binding protein OS=Entomoplasma luminosum OX=214888 GN=ssb PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84KK29 pKa = 9.39VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

866

0

866

306459

31

1855

353.9

40.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.962 ± 0.087

0.48 ± 0.019

5.483 ± 0.069

6.527 ± 0.08

5.154 ± 0.079

5.557 ± 0.082

1.592 ± 0.028

9.626 ± 0.095

9.501 ± 0.081

9.141 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.046

6.857 ± 0.08

2.947 ± 0.04

3.943 ± 0.052

2.725 ± 0.048

5.722 ± 0.062

5.727 ± 0.071

5.939 ± 0.062

1.203 ± 0.032

3.425 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski