Pseudoflavonifractor sp. AF19-9AC

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Pseudoflavonifractor; unclassified Pseudoflavonifractor

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2880 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A416RC94|A0A416RC94_9FIRM DUF1349 domain-containing protein OS=Pseudoflavonifractor sp. AF19-9AC OX=2292244 GN=DWX58_06350 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 8.69RR3 pKa = 11.84TKK5 pKa = 10.23YY6 pKa = 10.55RR7 pKa = 11.84SITVLLCALLVLSGCGARR25 pKa = 11.84TSEE28 pKa = 4.45EE29 pKa = 3.87TSSAQEE35 pKa = 4.01TSTSEE40 pKa = 3.69AAAVTTASTIDD51 pKa = 3.25TATIFSDD58 pKa = 3.45RR59 pKa = 11.84DD60 pKa = 3.26LEE62 pKa = 4.3GTYY65 pKa = 10.72DD66 pKa = 3.64EE67 pKa = 4.95SAAIAIQLSGDD78 pKa = 3.87SASCDD83 pKa = 3.16SDD85 pKa = 3.57AVTIEE90 pKa = 4.02GSRR93 pKa = 11.84ITIGAEE99 pKa = 3.73GVYY102 pKa = 10.37VLSGTLADD110 pKa = 4.01GQIVVNAGEE119 pKa = 4.08TDD121 pKa = 3.34KK122 pKa = 11.55VQLVLAGTDD131 pKa = 3.25ITSSTSAAIYY141 pKa = 10.4ALEE144 pKa = 4.12ADD146 pKa = 4.01KK147 pKa = 11.58VFVTLAEE154 pKa = 4.28GTEE157 pKa = 3.92NTLINGGEE165 pKa = 4.11YY166 pKa = 10.57VAIDD170 pKa = 4.58DD171 pKa = 4.38NNIDD175 pKa = 3.44AVIFAKK181 pKa = 10.09TDD183 pKa = 3.29LTLNGSGSLTVNAQAGHH200 pKa = 5.76GVVSKK205 pKa = 10.99DD206 pKa = 3.39DD207 pKa = 3.85LVITGGSYY215 pKa = 10.15TITAASHH222 pKa = 5.79GLSGKK227 pKa = 10.21DD228 pKa = 2.96VVAIADD234 pKa = 3.9GVFSITSGKK243 pKa = 10.48DD244 pKa = 3.0GIHH247 pKa = 6.93AEE249 pKa = 4.12NSDD252 pKa = 3.97DD253 pKa = 3.83LTLGTLYY260 pKa = 10.33IADD263 pKa = 3.88GSYY266 pKa = 10.68TITSQGDD273 pKa = 3.55TISAQGALQIDD284 pKa = 4.56GGTFDD289 pKa = 5.56LYY291 pKa = 10.65TGEE294 pKa = 4.72GSDD297 pKa = 3.9SVEE300 pKa = 4.19MTSGDD305 pKa = 3.17QFGQMGGGPRR315 pKa = 11.84GGIDD319 pKa = 3.24AQTVVTTTATEE330 pKa = 4.0EE331 pKa = 4.19DD332 pKa = 4.07TVSQKK337 pKa = 10.81GIKK340 pKa = 10.18GEE342 pKa = 4.04STYY345 pKa = 10.44TINGGVFTIDD355 pKa = 3.45SADD358 pKa = 3.71DD359 pKa = 3.6CLHH362 pKa = 6.73AGGVMTLAAGAFTLNSGDD380 pKa = 4.56DD381 pKa = 3.71AVHH384 pKa = 6.88CDD386 pKa = 4.34DD387 pKa = 4.82VLTIQSGTFTIPYY400 pKa = 8.75CYY402 pKa = 10.04EE403 pKa = 4.34GIEE406 pKa = 4.22GLSITIEE413 pKa = 4.58DD414 pKa = 3.85GTFDD418 pKa = 3.59ITSSDD423 pKa = 3.83DD424 pKa = 3.45GLNAAGGVDD433 pKa = 3.27SSGFGGQTQDD443 pKa = 2.96TFAADD448 pKa = 3.5SDD450 pKa = 4.26SFITINGGTLTIVSSGDD467 pKa = 3.71CVDD470 pKa = 3.84SNGALTINGGSLDD483 pKa = 3.92LTCNGSGNTTLDD495 pKa = 3.35CDD497 pKa = 3.75GTYY500 pKa = 11.0SNNGGDD506 pKa = 3.05ISTNDD511 pKa = 3.38GSEE514 pKa = 4.19DD515 pKa = 3.41NPGQMGGKK523 pKa = 10.16GGGQSGGGRR532 pKa = 11.84QDD534 pKa = 3.19GTNGGGRR541 pKa = 11.84PDD543 pKa = 3.63RR544 pKa = 11.84QTVPSASS551 pKa = 3.29

Molecular weight: 55.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A416REC9|A0A416REC9_9FIRM DNA polymerase III subunit delta OS=Pseudoflavonifractor sp. AF19-9AC OX=2292244 GN=DWX58_05520 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.6KK9 pKa = 7.95RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84VRR41 pKa = 11.84LTHH44 pKa = 6.42

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2880

0

2880

892399

25

2581

309.9

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.286 ± 0.05

1.629 ± 0.022

5.433 ± 0.039

6.936 ± 0.058

3.721 ± 0.031

8.012 ± 0.051

1.796 ± 0.021

5.369 ± 0.045

4.534 ± 0.047

10.436 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.025

3.198 ± 0.032

4.286 ± 0.033

3.867 ± 0.027

5.588 ± 0.049

5.69 ± 0.045

5.583 ± 0.056

7.25 ± 0.038

1.174 ± 0.018

3.437 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski