Amino acid dipepetide frequency for Pseudoflavonifractor sp. AF19-9AC

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.947AlaAla: 10.947 ± 0.155	1.526AlaCys: 1.526 ± 0.042	4.974AlaAsp: 4.974 ± 0.078	5.701AlaGlu: 5.701 ± 0.09	3.29AlaPhe: 3.29 ± 0.071	7.758AlaGly: 7.758 ± 0.092	1.471AlaHis: 1.471 ± 0.041	5.003AlaIle: 5.003 ± 0.096	3.94AlaLys: 3.94 ± 0.084	10.82AlaLeu: 10.82 ± 0.117	2.837AlaMet: 2.837 ± 0.049	2.325AlaAsn: 2.325 ± 0.055	3.577AlaPro: 3.577 ± 0.076	4.191AlaGln: 4.191 ± 0.069	4.894AlaArg: 4.894 ± 0.089	4.378AlaSer: 4.378 ± 0.07	3.73AlaThr: 3.73 ± 0.076	7.743AlaVal: 7.743 ± 0.105	1.041AlaTrp: 1.041 ± 0.033	2.712AlaTyr: 2.712 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	1.468CysAla: 1.468 ± 0.043	0.377CysCys: 0.377 ± 0.023	0.802CysAsp: 0.802 ± 0.031	0.779CysGlu: 0.779 ± 0.031	0.561CysPhe: 0.561 ± 0.025	1.814CysGly: 1.814 ± 0.052	0.345CysHis: 0.345 ± 0.022	0.812CysIle: 0.812 ± 0.031	0.6CysLys: 0.6 ± 0.027	1.596CysLeu: 1.596 ± 0.043	0.381CysMet: 0.381 ± 0.019	0.431CysAsn: 0.431 ± 0.023	0.882CysPro: 0.882 ± 0.035	0.61CysGln: 0.61 ± 0.025	1.054CysArg: 1.054 ± 0.037	0.93CysSer: 0.93 ± 0.033	0.954CysThr: 0.954 ± 0.036	1.16CysVal: 1.16 ± 0.036	0.187CysTrp: 0.187 ± 0.012	0.551CysTyr: 0.551 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	4.516AspAla: 4.516 ± 0.076	0.902AspCys: 0.902 ± 0.034	2.371AspAsp: 2.371 ± 0.067	3.636AspGlu: 3.636 ± 0.077	2.278AspPhe: 2.278 ± 0.046	4.917AspGly: 4.917 ± 0.092	1.086AspHis: 1.086 ± 0.035	3.142AspIle: 3.142 ± 0.073	2.489AspLys: 2.489 ± 0.058	5.374AspLeu: 5.374 ± 0.071	1.474AspMet: 1.474 ± 0.052	1.726AspAsn: 1.726 ± 0.043	2.582AspPro: 2.582 ± 0.059	1.954AspGln: 1.954 ± 0.047	3.086AspArg: 3.086 ± 0.061	2.849AspSer: 2.849 ± 0.055	3.148AspThr: 3.148 ± 0.067	3.701AspVal: 3.701 ± 0.065	0.783AspTrp: 0.783 ± 0.033	2.314AspTyr: 2.314 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	6.273GluAla: 6.273 ± 0.096	0.732GluCys: 0.732 ± 0.032	4.111GluAsp: 4.111 ± 0.08	6.656GluGlu: 6.656 ± 0.125	2.082GluPhe: 2.082 ± 0.055	5.188GluGly: 5.188 ± 0.083	1.465GluHis: 1.465 ± 0.04	3.668GluIle: 3.668 ± 0.072	4.035GluLys: 4.035 ± 0.074	7.318GluLeu: 7.318 ± 0.105	1.898GluMet: 1.898 ± 0.045	2.735GluAsn: 2.735 ± 0.058	2.361GluPro: 2.361 ± 0.064	3.482GluGln: 3.482 ± 0.075	4.126GluArg: 4.126 ± 0.084	2.849GluSer: 2.849 ± 0.059	3.511GluThr: 3.511 ± 0.064	4.129GluVal: 4.129 ± 0.069	0.622GluTrp: 0.622 ± 0.027	2.115GluTyr: 2.115 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	3.148PheAla: 3.148 ± 0.063	0.758PheCys: 0.758 ± 0.029	2.274PheAsp: 2.274 ± 0.051	1.914PheGlu: 1.914 ± 0.048	1.695PhePhe: 1.695 ± 0.049	3.131PheGly: 3.131 ± 0.06	0.816PheHis: 0.816 ± 0.032	1.69PheIle: 1.69 ± 0.047	1.239PheLys: 1.239 ± 0.037	4.018PheLeu: 4.018 ± 0.073	0.769PheMet: 0.769 ± 0.032	1.099PheAsn: 1.099 ± 0.039	1.59PhePro: 1.59 ± 0.048	1.509PheGln: 1.509 ± 0.04	1.972PheArg: 1.972 ± 0.052	2.787PheSer: 2.787 ± 0.06	2.344PheThr: 2.344 ± 0.06	2.567PheVal: 2.567 ± 0.055	0.501PheTrp: 0.501 ± 0.025	1.385PheTyr: 1.385 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	6.675GlyAla: 6.675 ± 0.098	1.46GlyCys: 1.46 ± 0.041	4.093GlyAsp: 4.093 ± 0.066	5.48GlyGlu: 5.48 ± 0.092	2.959GlyPhe: 2.959 ± 0.059	6.693GlyGly: 6.693 ± 0.137	1.406GlyHis: 1.406 ± 0.045	4.671GlyIle: 4.671 ± 0.076	4.299GlyLys: 4.299 ± 0.077	7.763GlyLeu: 7.763 ± 0.115	2.608GlyMet: 2.608 ± 0.06	2.499GlyAsn: 2.499 ± 0.067	2.178GlyPro: 2.178 ± 0.053	2.999GlyGln: 2.999 ± 0.06	4.393GlyArg: 4.393 ± 0.075	4.785GlySer: 4.785 ± 0.101	4.964GlyThr: 4.964 ± 0.123	6.128GlyVal: 6.128 ± 0.093	1.016GlyTrp: 1.016 ± 0.036	3.054GlyTyr: 3.054 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	1.321HisAla: 1.321 ± 0.045	0.354HisCys: 0.354 ± 0.021	0.808HisAsp: 0.808 ± 0.031	0.941HisGlu: 0.941 ± 0.035	0.704HisPhe: 0.704 ± 0.028	1.429HisGly: 1.429 ± 0.037	0.405HisHis: 0.405 ± 0.027	1.191HisIle: 1.191 ± 0.038	0.678HisLys: 0.678 ± 0.029	1.862HisLeu: 1.862 ± 0.042	0.521HisMet: 0.521 ± 0.025	0.614HisAsn: 0.614 ± 0.023	1.168HisPro: 1.168 ± 0.038	0.649HisGln: 0.649 ± 0.029	1.097HisArg: 1.097 ± 0.04	1.029HisSer: 1.029 ± 0.039	1.155HisThr: 1.155 ± 0.038	1.097HisVal: 1.097 ± 0.033	0.227HisTrp: 0.227 ± 0.017	0.712HisTyr: 0.712 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	4.978IleAla: 4.978 ± 0.079	0.978IleCys: 0.978 ± 0.033	2.924IleAsp: 2.924 ± 0.062	2.841IleGlu: 2.841 ± 0.058	2.037IlePhe: 2.037 ± 0.052	3.853IleGly: 3.853 ± 0.076	1.089IleHis: 1.089 ± 0.041	2.843IleIle: 2.843 ± 0.07	1.959IleLys: 1.959 ± 0.057	5.93IleLeu: 5.93 ± 0.094	1.274IleMet: 1.274 ± 0.043	1.774IleAsn: 1.774 ± 0.048	2.998IlePro: 2.998 ± 0.062	2.107IleGln: 2.107 ± 0.05	3.173IleArg: 3.173 ± 0.059	3.44IleSer: 3.44 ± 0.071	3.537IleThr: 3.537 ± 0.075	3.581IleVal: 3.581 ± 0.07	0.558IleTrp: 0.558 ± 0.028	1.821IleTyr: 1.821 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	4.395LysAla: 4.395 ± 0.084	0.502LysCys: 0.502 ± 0.026	2.454LysAsp: 2.454 ± 0.056	3.764LysGlu: 3.764 ± 0.08	1.201LysPhe: 1.201 ± 0.041	3.296LysGly: 3.296 ± 0.068	0.721LysHis: 0.721 ± 0.028	2.425LysIle: 2.425 ± 0.062	3.069LysLys: 3.069 ± 0.072	4.47LysLeu: 4.47 ± 0.079	1.268LysMet: 1.268 ± 0.049	1.758LysAsn: 1.758 ± 0.05	1.792LysPro: 1.792 ± 0.049	1.664LysGln: 1.664 ± 0.045	2.614LysArg: 2.614 ± 0.06	2.225LysSer: 2.225 ± 0.05	2.628LysThr: 2.628 ± 0.064	3.112LysVal: 3.112 ± 0.062	0.446LysTrp: 0.446 ± 0.024	1.534LysTyr: 1.534 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	10.075LeuAla: 10.075 ± 0.137	2.149LeuCys: 2.149 ± 0.052	5.913LeuAsp: 5.913 ± 0.084	7.537LeuGlu: 7.537 ± 0.114	3.951LeuPhe: 3.951 ± 0.077	7.647LeuGly: 7.647 ± 0.112	1.671LeuHis: 1.671 ± 0.051	4.944LeuIle: 4.944 ± 0.084	4.355LeuLys: 4.355 ± 0.074	11.04LeuLeu: 11.04 ± 0.174	2.862LeuMet: 2.862 ± 0.063	3.253LeuAsn: 3.253 ± 0.065	5.013LeuPro: 5.013 ± 0.073	3.064LeuGln: 3.064 ± 0.057	5.791LeuArg: 5.791 ± 0.093	7.376LeuSer: 7.376 ± 0.094	6.317LeuThr: 6.317 ± 0.098	6.944LeuVal: 6.944 ± 0.101	1.216LeuTrp: 1.216 ± 0.047	3.241LeuTyr: 3.241 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.883MetAla: 2.883 ± 0.067	0.335MetCys: 0.335 ± 0.018	1.764MetAsp: 1.764 ± 0.049	2.417MetGlu: 2.417 ± 0.056	0.823MetPhe: 0.823 ± 0.033	2.26MetGly: 2.26 ± 0.054	0.347MetHis: 0.347 ± 0.019	1.291MetIle: 1.291 ± 0.04	1.679MetLys: 1.679 ± 0.046	2.68MetLeu: 2.68 ± 0.062	0.784MetMet: 0.784 ± 0.033	1.105MetAsn: 1.105 ± 0.034	1.151MetPro: 1.151 ± 0.035	0.781MetGln: 0.781 ± 0.031	1.337MetArg: 1.337 ± 0.039	1.664MetSer: 1.664 ± 0.049	1.652MetThr: 1.652 ± 0.046	2.004MetVal: 2.004 ± 0.048	0.189MetTrp: 0.189 ± 0.014	0.6MetTyr: 0.6 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.674AsnAla: 2.674 ± 0.056	0.493AsnCys: 0.493 ± 0.025	1.406AsnAsp: 1.406 ± 0.043	1.747AsnGlu: 1.747 ± 0.043	1.181AsnPhe: 1.181 ± 0.04	2.855AsnGly: 2.855 ± 0.072	0.63AsnHis: 0.63 ± 0.027	1.991AsnIle: 1.991 ± 0.051	1.297AsnLys: 1.297 ± 0.041	3.32AsnLeu: 3.32 ± 0.059	0.917AsnMet: 0.917 ± 0.035	1.033AsnAsn: 1.033 ± 0.037	1.955AsnPro: 1.955 ± 0.051	1.2AsnGln: 1.2 ± 0.039	1.921AsnArg: 1.921 ± 0.049	1.689AsnSer: 1.689 ± 0.045	1.853AsnThr: 1.853 ± 0.057	2.213AsnVal: 2.213 ± 0.056	0.407AsnTrp: 0.407 ± 0.021	1.2AsnTyr: 1.2 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	4.025ProAla: 4.025 ± 0.074	0.593ProCys: 0.593 ± 0.023	2.816ProAsp: 2.816 ± 0.051	4.156ProGlu: 4.156 ± 0.079	1.673ProPhe: 1.673 ± 0.047	3.401ProGly: 3.401 ± 0.063	0.75ProHis: 0.75 ± 0.031	2.229ProIle: 2.229 ± 0.053	1.849ProLys: 1.849 ± 0.05	3.911ProLeu: 3.911 ± 0.069	1.122ProMet: 1.122 ± 0.039	1.258ProAsn: 1.258 ± 0.04	1.5ProPro: 1.5 ± 0.046	1.809ProGln: 1.809 ± 0.048	1.888ProArg: 1.888 ± 0.056	2.23ProSer: 2.23 ± 0.054	1.952ProThr: 1.952 ± 0.043	3.677ProVal: 3.677 ± 0.066	0.537ProTrp: 0.537 ± 0.025	1.481ProTyr: 1.481 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	3.999GlnAla: 3.999 ± 0.077	0.456GlnCys: 0.456 ± 0.024	1.818GlnAsp: 1.818 ± 0.051	3.18GlnGlu: 3.18 ± 0.063	1.238GlnPhe: 1.238 ± 0.036	2.755GlnGly: 2.755 ± 0.054	0.546GlnHis: 0.546 ± 0.025	2.094GlnIle: 2.094 ± 0.053	1.943GlnLys: 1.943 ± 0.042	3.847GlnLeu: 3.847 ± 0.07	1.203GlnMet: 1.203 ± 0.039	1.375GlnAsn: 1.375 ± 0.045	1.474GlnPro: 1.474 ± 0.05	1.608GlnGln: 1.608 ± 0.053	2.243GlnArg: 2.243 ± 0.047	2.05GlnSer: 2.05 ± 0.052	1.969GlnThr: 1.969 ± 0.05	3.049GlnVal: 3.049 ± 0.058	0.491GlnTrp: 0.491 ± 0.023	1.331GlnTyr: 1.331 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	4.668ArgAla: 4.668 ± 0.076	0.929ArgCys: 0.929 ± 0.034	2.918ArgAsp: 2.918 ± 0.06	4.478ArgGlu: 4.478 ± 0.08	2.158ArgPhe: 2.158 ± 0.048	3.572ArgGly: 3.572 ± 0.066	1.035ArgHis: 1.035 ± 0.038	2.982ArgIle: 2.982 ± 0.063	2.775ArgLys: 2.775 ± 0.07	5.736ArgLeu: 5.736 ± 0.089	1.739ArgMet: 1.739 ± 0.045	1.765ArgAsn: 1.765 ± 0.048	2.34ArgPro: 2.34 ± 0.05	2.571ArgGln: 2.571 ± 0.065	4.105ArgArg: 4.105 ± 0.076	2.861ArgSer: 2.861 ± 0.062	2.838ArgThr: 2.838 ± 0.057	3.599ArgVal: 3.599 ± 0.081	0.745ArgTrp: 0.745 ± 0.033	2.067ArgTyr: 2.067 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	5.366SerAla: 5.366 ± 0.086	0.831SerCys: 0.831 ± 0.031	2.935SerAsp: 2.935 ± 0.069	2.956SerGlu: 2.956 ± 0.059	2.398SerPhe: 2.398 ± 0.049	5.565SerGly: 5.565 ± 0.116	1.126SerHis: 1.126 ± 0.036	3.078SerIle: 3.078 ± 0.062	2.227SerLys: 2.227 ± 0.055	5.686SerLeu: 5.686 ± 0.089	1.607SerMet: 1.607 ± 0.039	1.7SerAsn: 1.7 ± 0.047	2.48SerPro: 2.48 ± 0.054	2.318SerGln: 2.318 ± 0.06	3.204SerArg: 3.204 ± 0.062	3.608SerSer: 3.608 ± 0.098	2.968SerThr: 2.968 ± 0.065	4.082SerVal: 4.082 ± 0.073	0.724SerTrp: 0.724 ± 0.03	2.036SerTyr: 2.036 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	5.635ThrAla: 5.635 ± 0.109	0.771ThrCys: 0.771 ± 0.037	2.945ThrAsp: 2.945 ± 0.063	3.085ThrGlu: 3.085 ± 0.072	2.184ThrPhe: 2.184 ± 0.052	5.099ThrGly: 5.099 ± 0.101	0.942ThrHis: 0.942 ± 0.032	3.405ThrIle: 3.405 ± 0.083	1.945ThrLys: 1.945 ± 0.046	5.961ThrLeu: 5.961 ± 0.092	1.463ThrMet: 1.463 ± 0.041	1.564ThrAsn: 1.564 ± 0.051	2.888ThrPro: 2.888 ± 0.057	1.942ThrGln: 1.942 ± 0.049	2.617ThrArg: 2.617 ± 0.062	2.748ThrSer: 2.748 ± 0.072	2.966ThrThr: 2.966 ± 0.075	5.127ThrVal: 5.127 ± 0.109	0.673ThrTrp: 0.673 ± 0.031	1.874ThrTyr: 1.874 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	5.993ValAla: 5.993 ± 0.105	1.362ValCys: 1.362 ± 0.04	4.106ValAsp: 4.106 ± 0.073	4.971ValGlu: 4.971 ± 0.085	2.822ValPhe: 2.822 ± 0.064	5.203ValGly: 5.203 ± 0.081	1.15ValHis: 1.15 ± 0.041	3.99ValIle: 3.99 ± 0.08	3.133ValLys: 3.133 ± 0.065	8.093ValLeu: 8.093 ± 0.116	1.945ValMet: 1.945 ± 0.05	2.292ValAsn: 2.292 ± 0.062	3.166ValPro: 3.166 ± 0.061	2.186ValGln: 2.186 ± 0.047	3.753ValArg: 3.753 ± 0.072	4.808ValSer: 4.808 ± 0.067	4.657ValThr: 4.657 ± 0.121	5.608ValVal: 5.608 ± 0.095	0.839ValTrp: 0.839 ± 0.028	2.424ValTyr: 2.424 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	1.021TrpAla: 1.021 ± 0.038	0.184TrpCys: 0.184 ± 0.016	0.676TrpAsp: 0.676 ± 0.03	0.881TrpGlu: 0.881 ± 0.029	0.5TrpPhe: 0.5 ± 0.024	0.952TrpGly: 0.952 ± 0.035	0.203TrpHis: 0.203 ± 0.015	0.504TrpIle: 0.504 ± 0.024	0.589TrpLys: 0.589 ± 0.026	1.341TrpLeu: 1.341 ± 0.045	0.36TrpMet: 0.36 ± 0.02	0.478TrpAsn: 0.478 ± 0.025	0.369TrpPro: 0.369 ± 0.024	0.551TrpGln: 0.551 ± 0.023	0.615TrpArg: 0.615 ± 0.029	0.648TrpSer: 0.648 ± 0.031	0.569TrpThr: 0.569 ± 0.028	0.7TrpVal: 0.7 ± 0.028	0.139TrpTrp: 0.139 ± 0.013	0.459TrpTyr: 0.459 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.799TyrAla: 2.799 ± 0.05	0.603TyrCys: 0.603 ± 0.024	2.22TyrAsp: 2.22 ± 0.049	2.236TyrGlu: 2.236 ± 0.054	1.47TyrPhe: 1.47 ± 0.043	2.735TyrGly: 2.735 ± 0.051	0.759TyrHis: 0.759 ± 0.033	1.739TyrIle: 1.739 ± 0.046	1.24TyrLys: 1.24 ± 0.038	3.589TyrLeu: 3.589 ± 0.074	0.712TyrMet: 0.712 ± 0.028	1.197TyrAsn: 1.197 ± 0.042	1.394TyrPro: 1.394 ± 0.044	1.476TyrGln: 1.476 ± 0.045	2.0TyrArg: 2.0 ± 0.051	1.944TyrSer: 1.944 ± 0.045	2.122TyrThr: 2.122 ± 0.063	2.277TyrVal: 2.277 ± 0.05	0.398TyrTrp: 0.398 ± 0.025	1.462TyrTyr: 1.462 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2880 proteins (892399 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---