Amino acid dipepetide frequency for Dictyostelium purpureum (Slime mold)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.003AlaAla: 2.003 ± 0.032	0.56AlaCys: 0.56 ± 0.012	1.553AlaAsp: 1.553 ± 0.02	1.734AlaGlu: 1.734 ± 0.023	1.678AlaPhe: 1.678 ± 0.017	1.734AlaGly: 1.734 ± 0.032	0.636AlaHis: 0.636 ± 0.011	3.018AlaIle: 3.018 ± 0.026	2.388AlaLys: 2.388 ± 0.021	3.287AlaLeu: 3.287 ± 0.031	0.676AlaMet: 0.676 ± 0.012	2.291AlaAsn: 2.291 ± 0.025	1.631AlaPro: 1.631 ± 0.026	1.333AlaGln: 1.333 ± 0.022	1.094AlaArg: 1.094 ± 0.02	3.315AlaSer: 3.315 ± 0.033	2.366AlaThr: 2.366 ± 0.029	1.803AlaVal: 1.803 ± 0.022	0.256AlaTrp: 0.256 ± 0.008	1.114AlaTyr: 1.114 ± 0.014	0.0AlaXaa: 0.0 ± 0.0
Cys	0.517CysAla: 0.517 ± 0.012	0.301CysCys: 0.301 ± 0.011	0.799CysAsp: 0.799 ± 0.018	0.726CysGlu: 0.726 ± 0.012	0.856CysPhe: 0.856 ± 0.014	0.869CysGly: 0.869 ± 0.016	0.274CysHis: 0.274 ± 0.007	1.168CysIle: 1.168 ± 0.014	1.153CysLys: 1.153 ± 0.018	1.33CysLeu: 1.33 ± 0.016	0.218CysMet: 0.218 ± 0.006	1.124CysAsn: 1.124 ± 0.021	0.626CysPro: 0.626 ± 0.015	0.552CysGln: 0.552 ± 0.01	0.458CysArg: 0.458 ± 0.009	1.428CysSer: 1.428 ± 0.024	0.827CysThr: 0.827 ± 0.019	0.729CysVal: 0.729 ± 0.013	0.142CysTrp: 0.142 ± 0.005	0.613CysTyr: 0.613 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	1.594AspAla: 1.594 ± 0.019	0.856AspCys: 0.856 ± 0.017	3.575AspAsp: 3.575 ± 0.042	3.953AspGlu: 3.953 ± 0.039	2.906AspPhe: 2.906 ± 0.024	2.482AspGly: 2.482 ± 0.029	1.043AspHis: 1.043 ± 0.013	4.65AspIle: 4.65 ± 0.032	3.679AspLys: 3.679 ± 0.03	4.77AspLeu: 4.77 ± 0.03	0.796AspMet: 0.796 ± 0.012	4.184AspAsn: 4.184 ± 0.032	2.048AspPro: 2.048 ± 0.018	2.416AspGln: 2.416 ± 0.021	1.669AspArg: 1.669 ± 0.025	4.862AspSer: 4.862 ± 0.05	2.447AspThr: 2.447 ± 0.021	2.162AspVal: 2.162 ± 0.019	0.45AspTrp: 0.45 ± 0.01	2.346AspTyr: 2.346 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	2.125GluAla: 2.125 ± 0.027	0.929GluCys: 0.929 ± 0.018	3.567GluAsp: 3.567 ± 0.035	5.912GluGlu: 5.912 ± 0.069	2.946GluPhe: 2.946 ± 0.024	2.209GluGly: 2.209 ± 0.024	0.956GluHis: 0.956 ± 0.014	5.556GluIle: 5.556 ± 0.037	6.24GluLys: 6.24 ± 0.062	5.132GluLeu: 5.132 ± 0.036	1.209GluMet: 1.209 ± 0.016	5.146GluAsn: 5.146 ± 0.037	1.75GluPro: 1.75 ± 0.021	2.614GluGln: 2.614 ± 0.033	2.486GluArg: 2.486 ± 0.043	4.737GluSer: 4.737 ± 0.034	3.13GluThr: 3.13 ± 0.027	2.481GluVal: 2.481 ± 0.024	0.536GluTrp: 0.536 ± 0.011	2.481GluTyr: 2.481 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	1.552PheAla: 1.552 ± 0.018	0.735PheCys: 0.735 ± 0.012	2.832PheAsp: 2.832 ± 0.024	2.891PheGlu: 2.891 ± 0.023	2.572PhePhe: 2.572 ± 0.03	2.842PheGly: 2.842 ± 0.036	0.828PheHis: 0.828 ± 0.013	4.311PheIle: 4.311 ± 0.033	4.358PheLys: 4.358 ± 0.036	4.429PheLeu: 4.429 ± 0.039	0.714PheMet: 0.714 ± 0.011	4.652PheAsn: 4.652 ± 0.049	1.606PhePro: 1.606 ± 0.016	1.768PheGln: 1.768 ± 0.018	1.374PheArg: 1.374 ± 0.016	4.661PheSer: 4.661 ± 0.031	2.497PheThr: 2.497 ± 0.025	2.221PheVal: 2.221 ± 0.023	0.389PheTrp: 0.389 ± 0.009	2.167PheTyr: 2.167 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	1.941GlyAla: 1.941 ± 0.035	0.832GlyCys: 0.832 ± 0.017	2.546GlyAsp: 2.546 ± 0.026	2.37GlyGlu: 2.37 ± 0.026	2.443GlyPhe: 2.443 ± 0.021	3.023GlyGly: 3.023 ± 0.039	0.947GlyHis: 0.947 ± 0.017	3.404GlyIle: 3.404 ± 0.029	3.005GlyLys: 3.005 ± 0.026	3.637GlyLeu: 3.637 ± 0.03	0.718GlyMet: 0.718 ± 0.013	3.122GlyAsn: 3.122 ± 0.029	1.176GlyPro: 1.176 ± 0.021	1.409GlyGln: 1.409 ± 0.017	1.383GlyArg: 1.383 ± 0.018	4.456GlySer: 4.456 ± 0.047	2.562GlyThr: 2.562 ± 0.032	2.593GlyVal: 2.593 ± 0.026	0.466GlyTrp: 0.466 ± 0.012	1.995GlyTyr: 1.995 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	0.51HisAla: 0.51 ± 0.01	0.349HisCys: 0.349 ± 0.008	0.736HisAsp: 0.736 ± 0.011	0.765HisGlu: 0.765 ± 0.012	1.064HisPhe: 1.064 ± 0.015	0.825HisGly: 0.825 ± 0.015	0.88HisHis: 0.88 ± 0.027	1.423HisIle: 1.423 ± 0.016	1.139HisLys: 1.139 ± 0.016	2.06HisLeu: 2.06 ± 0.021	0.291HisMet: 0.291 ± 0.007	1.34HisAsn: 1.34 ± 0.017	0.985HisPro: 0.985 ± 0.016	1.195HisGln: 1.195 ± 0.02	0.726HisArg: 0.726 ± 0.012	1.81HisSer: 1.81 ± 0.02	0.893HisThr: 0.893 ± 0.016	0.702HisVal: 0.702 ± 0.011	0.18HisTrp: 0.18 ± 0.005	0.924HisTyr: 0.924 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	2.813IleAla: 2.813 ± 0.026	1.164IleCys: 1.164 ± 0.017	4.955IleAsp: 4.955 ± 0.034	5.603IleGlu: 5.603 ± 0.04	3.953IlePhe: 3.953 ± 0.036	3.607IleGly: 3.607 ± 0.032	1.548IleHis: 1.548 ± 0.019	6.943IleIle: 6.943 ± 0.049	7.446IleLys: 7.446 ± 0.044	7.265IleLeu: 7.265 ± 0.045	1.175IleMet: 1.175 ± 0.016	7.491IleAsn: 7.491 ± 0.055	3.824IlePro: 3.824 ± 0.036	3.476IleGln: 3.476 ± 0.028	2.366IleArg: 2.366 ± 0.02	7.333IleSer: 7.333 ± 0.038	4.457IleThr: 4.457 ± 0.031	4.152IleVal: 4.152 ± 0.026	0.548IleTrp: 0.548 ± 0.011	3.05IleTyr: 3.05 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	2.525LysAla: 2.525 ± 0.023	1.127LysCys: 1.127 ± 0.018	4.573LysAsp: 4.573 ± 0.033	6.901LysGlu: 6.901 ± 0.06	3.261LysPhe: 3.261 ± 0.025	2.78LysGly: 2.78 ± 0.028	1.363LysHis: 1.363 ± 0.015	6.281LysIle: 6.281 ± 0.044	8.098LysLys: 8.098 ± 0.063	6.917LysLeu: 6.917 ± 0.044	1.472LysMet: 1.472 ± 0.015	6.314LysAsn: 6.314 ± 0.042	3.001LysPro: 3.001 ± 0.027	3.471LysGln: 3.471 ± 0.032	2.828LysArg: 2.828 ± 0.028	6.648LysSer: 6.648 ± 0.038	4.093LysThr: 4.093 ± 0.032	3.705LysVal: 3.705 ± 0.026	0.616LysTrp: 0.616 ± 0.01	3.304LysTyr: 3.304 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	3.286LeuAla: 3.286 ± 0.034	1.175LeuCys: 1.175 ± 0.016	4.729LeuAsp: 4.729 ± 0.034	6.032LeuGlu: 6.032 ± 0.041	4.832LeuPhe: 4.832 ± 0.034	3.633LeuGly: 3.633 ± 0.029	1.482LeuHis: 1.482 ± 0.016	6.872LeuIle: 6.872 ± 0.046	8.097LeuLys: 8.097 ± 0.052	8.802LeuLeu: 8.802 ± 0.058	1.49LeuMet: 1.49 ± 0.016	7.234LeuAsn: 7.234 ± 0.044	4.26LeuPro: 4.26 ± 0.06	3.414LeuGln: 3.414 ± 0.03	2.609LeuArg: 2.609 ± 0.027	8.331LeuSer: 8.331 ± 0.049	4.943LeuThr: 4.943 ± 0.064	4.625LeuVal: 4.625 ± 0.036	0.616LeuTrp: 0.616 ± 0.011	3.517LeuTyr: 3.517 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	0.864MetAla: 0.864 ± 0.013	0.207MetCys: 0.207 ± 0.006	1.175MetAsp: 1.175 ± 0.015	1.242MetGlu: 1.242 ± 0.016	0.757MetPhe: 0.757 ± 0.013	0.95MetGly: 0.95 ± 0.014	0.2MetHis: 0.2 ± 0.006	1.26MetIle: 1.26 ± 0.017	1.363MetLys: 1.363 ± 0.017	1.224MetLeu: 1.224 ± 0.013	0.357MetMet: 0.357 ± 0.009	1.385MetAsn: 1.385 ± 0.021	0.451MetPro: 0.451 ± 0.009	0.442MetGln: 0.442 ± 0.01	0.422MetArg: 0.422 ± 0.009	1.244MetSer: 1.244 ± 0.016	0.76MetThr: 0.76 ± 0.012	1.154MetVal: 1.154 ± 0.016	0.111MetTrp: 0.111 ± 0.004	0.508MetTyr: 0.508 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	2.072AsnAla: 2.072 ± 0.022	1.136AsnCys: 1.136 ± 0.019	4.398AsnAsp: 4.398 ± 0.035	4.922AsnGlu: 4.922 ± 0.033	3.544AsnPhe: 3.544 ± 0.03	3.807AsnGly: 3.807 ± 0.034	1.785AsnHis: 1.785 ± 0.019	7.862AsnIle: 7.862 ± 0.053	6.734AsnLys: 6.734 ± 0.045	6.591AsnLeu: 6.591 ± 0.043	1.211AsnMet: 1.211 ± 0.018	18.509AsnAsn: 18.509 ± 0.254	2.762AsnPro: 2.762 ± 0.027	4.668AsnGln: 4.668 ± 0.051	2.306AsnArg: 2.306 ± 0.022	9.354AsnSer: 9.354 ± 0.076	4.529AsnThr: 4.529 ± 0.034	3.144AsnVal: 3.144 ± 0.025	0.524AsnTrp: 0.524 ± 0.01	3.469AsnTyr: 3.469 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	1.429ProAla: 1.429 ± 0.025	0.425ProCys: 0.425 ± 0.011	1.679ProAsp: 1.679 ± 0.02	2.064ProGlu: 2.064 ± 0.022	1.946ProPhe: 1.946 ± 0.024	1.58ProGly: 1.58 ± 0.034	0.837ProHis: 0.837 ± 0.013	3.623ProIle: 3.623 ± 0.028	2.85ProLys: 2.85 ± 0.026	3.693ProLeu: 3.693 ± 0.027	0.617ProMet: 0.617 ± 0.011	3.716ProAsn: 3.716 ± 0.046	2.91ProPro: 2.91 ± 0.058	2.249ProGln: 2.249 ± 0.032	1.011ProArg: 1.011 ± 0.017	4.475ProSer: 4.475 ± 0.041	3.163ProThr: 3.163 ± 0.036	1.932ProVal: 1.932 ± 0.024	0.243ProTrp: 0.243 ± 0.007	1.364ProTyr: 1.364 ± 0.016	0.0ProXaa: 0.0 ± 0.0
Gln	1.359GlnAla: 1.359 ± 0.019	0.606GlnCys: 0.606 ± 0.012	1.934GlnAsp: 1.934 ± 0.019	2.619GlnGlu: 2.619 ± 0.03	2.05GlnPhe: 2.05 ± 0.021	1.224GlnGly: 1.224 ± 0.017	1.048GlnHis: 1.048 ± 0.018	3.445GlnIle: 3.445 ± 0.026	2.996GlnLys: 2.996 ± 0.028	4.321GlnLeu: 4.321 ± 0.035	0.853GlnMet: 0.853 ± 0.014	3.213GlnAsn: 3.213 ± 0.03	2.312GlnPro: 2.312 ± 0.038	5.679GlnGln: 5.679 ± 0.115	1.351GlnArg: 1.351 ± 0.019	3.571GlnSer: 3.571 ± 0.03	2.278GlnThr: 2.278 ± 0.023	2.115GlnVal: 2.115 ± 0.029	0.348GlnTrp: 0.348 ± 0.008	1.796GlnTyr: 1.796 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	1.251ArgAla: 1.251 ± 0.019	0.46ArgCys: 0.46 ± 0.009	1.855ArgAsp: 1.855 ± 0.027	2.269ArgGlu: 2.269 ± 0.033	1.577ArgPhe: 1.577 ± 0.016	1.413ArgGly: 1.413 ± 0.019	0.515ArgHis: 0.515 ± 0.01	2.357ArgIle: 2.357 ± 0.022	2.471ArgLys: 2.471 ± 0.025	2.807ArgLeu: 2.807 ± 0.029	0.57ArgMet: 0.57 ± 0.011	2.197ArgAsn: 2.197 ± 0.02	0.993ArgPro: 0.993 ± 0.014	1.012ArgGln: 1.012 ± 0.016	1.191ArgArg: 1.191 ± 0.017	2.356ArgSer: 2.356 ± 0.028	1.34ArgThr: 1.34 ± 0.014	1.792ArgVal: 1.792 ± 0.021	0.268ArgTrp: 0.268 ± 0.008	1.301ArgTyr: 1.301 ± 0.015	0.0ArgXaa: 0.0 ± 0.0
Ser	3.099SerAla: 3.099 ± 0.032	1.213SerCys: 1.213 ± 0.023	4.37SerAsp: 4.37 ± 0.036	4.145SerGlu: 4.145 ± 0.034	4.879SerPhe: 4.879 ± 0.035	4.008SerGly: 4.008 ± 0.041	1.558SerHis: 1.558 ± 0.019	8.536SerIle: 8.536 ± 0.053	6.685SerLys: 6.685 ± 0.041	8.915SerLeu: 8.915 ± 0.058	1.389SerMet: 1.389 ± 0.015	9.495SerAsn: 9.495 ± 0.062	4.515SerPro: 4.515 ± 0.047	3.396SerGln: 3.396 ± 0.03	2.37SerArg: 2.37 ± 0.027	12.132SerSer: 12.132 ± 0.128	6.097SerThr: 6.097 ± 0.049	4.154SerVal: 4.154 ± 0.032	0.583SerTrp: 0.583 ± 0.012	3.078SerTyr: 3.078 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	2.101ThrAla: 2.101 ± 0.027	0.83ThrCys: 0.83 ± 0.018	2.345ThrAsp: 2.345 ± 0.026	2.581ThrGlu: 2.581 ± 0.025	2.916ThrPhe: 2.916 ± 0.036	2.432ThrGly: 2.432 ± 0.03	1.0ThrHis: 1.0 ± 0.013	5.21ThrIle: 5.21 ± 0.036	3.759ThrLys: 3.759 ± 0.031	5.618ThrLeu: 5.618 ± 0.064	0.811ThrMet: 0.811 ± 0.013	4.594ThrAsn: 4.594 ± 0.033	3.335ThrPro: 3.335 ± 0.039	2.092ThrGln: 2.092 ± 0.022	1.333ThrArg: 1.333 ± 0.015	5.409ThrSer: 5.409 ± 0.044	5.56ThrThr: 5.56 ± 0.078	2.659ThrVal: 2.659 ± 0.024	0.384ThrTrp: 0.384 ± 0.008	1.672ThrTyr: 1.672 ± 0.017	0.0ThrXaa: 0.0 ± 0.0
Val	2.056ValAla: 2.056 ± 0.025	0.847ValCys: 0.847 ± 0.013	2.65ValAsp: 2.65 ± 0.027	2.933ValGlu: 2.933 ± 0.03	2.499ValPhe: 2.499 ± 0.023	2.381ValGly: 2.381 ± 0.028	0.87ValHis: 0.87 ± 0.014	3.62ValIle: 3.62 ± 0.028	3.372ValLys: 3.372 ± 0.026	4.623ValLeu: 4.623 ± 0.034	0.777ValMet: 0.777 ± 0.014	3.274ValAsn: 3.274 ± 0.025	1.983ValPro: 1.983 ± 0.023	1.738ValGln: 1.738 ± 0.019	1.358ValArg: 1.358 ± 0.018	4.216ValSer: 4.216 ± 0.026	2.49ValThr: 2.49 ± 0.026	2.936ValVal: 2.936 ± 0.032	0.444ValTrp: 0.444 ± 0.012	1.958ValTyr: 1.958 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	0.313TrpAla: 0.313 ± 0.009	0.14TrpCys: 0.14 ± 0.006	0.508TrpAsp: 0.508 ± 0.011	0.487TrpGlu: 0.487 ± 0.01	0.41TrpPhe: 0.41 ± 0.009	0.404TrpGly: 0.404 ± 0.01	0.108TrpHis: 0.108 ± 0.005	0.586TrpIle: 0.586 ± 0.011	0.606TrpLys: 0.606 ± 0.01	0.675TrpLeu: 0.675 ± 0.011	0.16TrpMet: 0.16 ± 0.006	0.553TrpAsn: 0.553 ± 0.01	0.162TrpPro: 0.162 ± 0.005	0.186TrpGln: 0.186 ± 0.006	0.337TrpArg: 0.337 ± 0.008	0.661TrpSer: 0.661 ± 0.012	0.368TrpThr: 0.368 ± 0.009	0.47TrpVal: 0.47 ± 0.01	0.097TrpTrp: 0.097 ± 0.005	0.294TrpTyr: 0.294 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.06TyrAla: 1.06 ± 0.015	0.817TyrCys: 0.817 ± 0.023	2.107TyrAsp: 2.107 ± 0.021	1.992TyrGlu: 1.992 ± 0.021	2.308TyrPhe: 2.308 ± 0.021	1.827TyrGly: 1.827 ± 0.021	0.921TyrHis: 0.921 ± 0.013	3.042TyrIle: 3.042 ± 0.028	2.837TyrLys: 2.837 ± 0.024	3.881TyrLeu: 3.881 ± 0.035	0.57TyrMet: 0.57 ± 0.009	3.496TyrAsn: 3.496 ± 0.034	1.479TyrPro: 1.479 ± 0.018	2.009TyrGln: 2.009 ± 0.023	1.326TyrArg: 1.326 ± 0.015	3.617TyrSer: 3.617 ± 0.031	1.841TyrThr: 1.841 ± 0.021	1.493TyrVal: 1.493 ± 0.016	0.325TyrTrp: 0.325 ± 0.008	2.056TyrTyr: 2.056 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 12347 proteins (5964926 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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