Amino acid dipepetide frequency for Streptomyces sp. MP131-18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
25.017AlaAla: 25.017 ± 0.168
1.151AlaCys: 1.151 ± 0.026
9.106AlaAsp: 9.106 ± 0.066
9.989AlaGlu: 9.989 ± 0.087
3.638AlaPhe: 3.638 ± 0.045
14.847AlaGly: 14.847 ± 0.086
3.064AlaHis: 3.064 ± 0.042
3.32AlaIle: 3.32 ± 0.043
1.791AlaLys: 1.791 ± 0.037
15.114AlaLeu: 15.114 ± 0.095
2.466AlaMet: 2.466 ± 0.036
1.764AlaAsn: 1.764 ± 0.029
7.626AlaPro: 7.626 ± 0.076
3.584AlaGln: 3.584 ± 0.045
11.424AlaArg: 11.424 ± 0.086
5.236AlaSer: 5.236 ± 0.043
6.823AlaThr: 6.823 ± 0.052
12.443AlaVal: 12.443 ± 0.083
2.035AlaTrp: 2.035 ± 0.028
2.48AlaTyr: 2.48 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
1.142CysAla: 1.142 ± 0.024
0.099CysCys: 0.099 ± 0.007
0.474CysAsp: 0.474 ± 0.015
0.4CysGlu: 0.4 ± 0.013
0.214CysPhe: 0.214 ± 0.01
1.008CysGly: 1.008 ± 0.023
0.211CysHis: 0.211 ± 0.01
0.177CysIle: 0.177 ± 0.009
0.082CysLys: 0.082 ± 0.007
0.751CysLeu: 0.751 ± 0.018
0.113CysMet: 0.113 ± 0.007
0.13CysAsn: 0.13 ± 0.008
0.504CysPro: 0.504 ± 0.017
0.167CysGln: 0.167 ± 0.009
0.683CysArg: 0.683 ± 0.016
0.4CysSer: 0.4 ± 0.014
0.509CysThr: 0.509 ± 0.017
0.624CysVal: 0.624 ± 0.017
0.145CysTrp: 0.145 ± 0.008
0.158CysTyr: 0.158 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.157AspAla: 8.157 ± 0.065
0.435AspCys: 0.435 ± 0.016
3.539AspAsp: 3.539 ± 0.056
3.953AspGlu: 3.953 ± 0.046
1.685AspPhe: 1.685 ± 0.03
6.65AspGly: 6.65 ± 0.081
1.447AspHis: 1.447 ± 0.025
1.931AspIle: 1.931 ± 0.032
0.698AspLys: 0.698 ± 0.018
6.155AspLeu: 6.155 ± 0.061
0.858AspMet: 0.858 ± 0.019
0.912AspAsn: 0.912 ± 0.027
4.548AspPro: 4.548 ± 0.054
1.501AspGln: 1.501 ± 0.025
5.051AspArg: 5.051 ± 0.048
2.404AspSer: 2.404 ± 0.037
3.173AspThr: 3.173 ± 0.038
4.747AspVal: 4.747 ± 0.043
1.093AspTrp: 1.093 ± 0.024
1.208AspTyr: 1.208 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.613GluAla: 7.613 ± 0.071
0.356GluCys: 0.356 ± 0.012
3.013GluAsp: 3.013 ± 0.04
4.063GluGlu: 4.063 ± 0.051
1.521GluPhe: 1.521 ± 0.024
4.555GluGly: 4.555 ± 0.05
1.596GluHis: 1.596 ± 0.031
2.127GluIle: 2.127 ± 0.035
0.969GluLys: 0.969 ± 0.024
6.917GluLeu: 6.917 ± 0.057
0.88GluMet: 0.88 ± 0.018
1.047GluAsn: 1.047 ± 0.023
3.934GluPro: 3.934 ± 0.058
2.264GluGln: 2.264 ± 0.035
6.315GluArg: 6.315 ± 0.068
2.671GluSer: 2.671 ± 0.038
2.995GluThr: 2.995 ± 0.036
4.578GluVal: 4.578 ± 0.049
0.836GluTrp: 0.836 ± 0.018
1.108GluTyr: 1.108 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.77PheAla: 3.77 ± 0.048
0.249PheCys: 0.249 ± 0.009
2.034PheAsp: 2.034 ± 0.032
1.43PheGlu: 1.43 ± 0.026
0.854PhePhe: 0.854 ± 0.021
2.958PheGly: 2.958 ± 0.045
0.651PheHis: 0.651 ± 0.017
0.714PheIle: 0.714 ± 0.018
0.322PheLys: 0.322 ± 0.013
2.556PheLeu: 2.556 ± 0.039
0.37PheMet: 0.37 ± 0.012
0.532PheAsn: 0.532 ± 0.016
1.417PhePro: 1.417 ± 0.025
0.662PheGln: 0.662 ± 0.018
1.969PheArg: 1.969 ± 0.031
1.413PheSer: 1.413 ± 0.027
1.981PheThr: 1.981 ± 0.031
2.063PheVal: 2.063 ± 0.034
0.424PheTrp: 0.424 ± 0.014
0.519PheTyr: 0.519 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
11.875GlyAla: 11.875 ± 0.093
0.873GlyCys: 0.873 ± 0.021
5.417GlyAsp: 5.417 ± 0.058
5.911GlyGlu: 5.911 ± 0.061
2.764GlyPhe: 2.764 ± 0.041
9.667GlyGly: 9.667 ± 0.114
2.532GlyHis: 2.532 ± 0.039
3.355GlyIle: 3.355 ± 0.041
1.643GlyLys: 1.643 ± 0.029
9.831GlyLeu: 9.831 ± 0.068
2.003GlyMet: 2.003 ± 0.03
1.754GlyAsn: 1.754 ± 0.032
5.903GlyPro: 5.903 ± 0.062
2.776GlyGln: 2.776 ± 0.039
8.653GlyArg: 8.653 ± 0.079
5.074GlySer: 5.074 ± 0.057
6.443GlyThr: 6.443 ± 0.063
7.271GlyVal: 7.271 ± 0.063
1.694GlyTrp: 1.694 ± 0.028
2.169GlyTyr: 2.169 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.964HisAla: 2.964 ± 0.039
0.222HisCys: 0.222 ± 0.01
1.358HisAsp: 1.358 ± 0.027
1.298HisGlu: 1.298 ± 0.025
0.657HisPhe: 0.657 ± 0.02
2.598HisGly: 2.598 ± 0.044
0.759HisHis: 0.759 ± 0.022
0.675HisIle: 0.675 ± 0.019
0.266HisLys: 0.266 ± 0.011
2.657HisLeu: 2.657 ± 0.036
0.326HisMet: 0.326 ± 0.012
0.387HisAsn: 0.387 ± 0.014
1.878HisPro: 1.878 ± 0.03
0.651HisGln: 0.651 ± 0.019
2.415HisArg: 2.415 ± 0.035
0.977HisSer: 0.977 ± 0.019
1.31HisThr: 1.31 ± 0.027
1.8HisVal: 1.8 ± 0.031
0.382HisTrp: 0.382 ± 0.015
0.492HisTyr: 0.492 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.616IleAla: 4.616 ± 0.047
0.273IleCys: 0.273 ± 0.01
2.197IleAsp: 2.197 ± 0.029
1.937IleGlu: 1.937 ± 0.03
0.695IlePhe: 0.695 ± 0.021
3.405IleGly: 3.405 ± 0.044
0.618IleHis: 0.618 ± 0.016
0.864IleIle: 0.864 ± 0.022
0.498IleLys: 0.498 ± 0.015
2.188IleLeu: 2.188 ± 0.037
0.422IleMet: 0.422 ± 0.015
0.708IleAsn: 0.708 ± 0.018
1.665IlePro: 1.665 ± 0.028
0.655IleGln: 0.655 ± 0.018
2.31IleArg: 2.31 ± 0.032
1.597IleSer: 1.597 ± 0.029
2.125IleThr: 2.125 ± 0.031
2.537IleVal: 2.537 ± 0.042
0.38IleTrp: 0.38 ± 0.012
0.528IleTyr: 0.528 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
1.8LysAla: 1.8 ± 0.031
0.083LysCys: 0.083 ± 0.006
0.824LysAsp: 0.824 ± 0.021
0.863LysGlu: 0.863 ± 0.024
0.311LysPhe: 0.311 ± 0.012
1.095LysGly: 1.095 ± 0.023
0.325LysHis: 0.325 ± 0.013
0.58LysIle: 0.58 ± 0.019
0.421LysLys: 0.421 ± 0.02
1.342LysLeu: 1.342 ± 0.028
0.258LysMet: 0.258 ± 0.01
0.316LysAsn: 0.316 ± 0.014
0.894LysPro: 0.894 ± 0.02
0.446LysGln: 0.446 ± 0.014
1.174LysArg: 1.174 ± 0.022
0.774LysSer: 0.774 ± 0.021
0.868LysThr: 0.868 ± 0.022
1.117LysVal: 1.117 ± 0.026
0.197LysTrp: 0.197 ± 0.009
0.275LysTyr: 0.275 ± 0.012
0.0LysXaa: 0.0 ± 0.0
Leu
16.474LeuAla: 16.474 ± 0.096
0.895LeuCys: 0.895 ± 0.021
6.82LeuAsp: 6.82 ± 0.056
4.239LeuGlu: 4.239 ± 0.044
2.552LeuPhe: 2.552 ± 0.036
9.483LeuGly: 9.483 ± 0.066
2.471LeuHis: 2.471 ± 0.038
3.318LeuIle: 3.318 ± 0.04
1.366LeuLys: 1.366 ± 0.026
11.719LeuLeu: 11.719 ± 0.103
1.62LeuMet: 1.62 ± 0.025
1.63LeuAsn: 1.63 ± 0.028
6.918LeuPro: 6.918 ± 0.06
1.92LeuGln: 1.92 ± 0.031
9.331LeuArg: 9.331 ± 0.077
5.126LeuSer: 5.126 ± 0.057
6.988LeuThr: 6.988 ± 0.062
8.322LeuVal: 8.322 ± 0.066
1.352LeuTrp: 1.352 ± 0.024
1.756LeuTyr: 1.756 ± 0.029
0.001LeuXaa: 0.001 ± 0.001
Met
2.341MetAla: 2.341 ± 0.032
0.139MetCys: 0.139 ± 0.008
0.834MetAsp: 0.834 ± 0.019
0.714MetGlu: 0.714 ± 0.017
0.447MetPhe: 0.447 ± 0.016
1.197MetGly: 1.197 ± 0.024
0.353MetHis: 0.353 ± 0.012
0.631MetIle: 0.631 ± 0.018
0.304MetLys: 0.304 ± 0.012
1.654MetLeu: 1.654 ± 0.03
0.285MetMet: 0.285 ± 0.011
0.45MetAsn: 0.45 ± 0.014
1.105MetPro: 1.105 ± 0.022
0.416MetGln: 0.416 ± 0.015
1.466MetArg: 1.466 ± 0.026
1.296MetSer: 1.296 ± 0.025
1.542MetThr: 1.542 ± 0.029
1.145MetVal: 1.145 ± 0.025
0.216MetTrp: 0.216 ± 0.009
0.296MetTyr: 0.296 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.207AsnAla: 2.207 ± 0.032
0.137AsnCys: 0.137 ± 0.007
0.992AsnAsp: 0.992 ± 0.024
0.927AsnGlu: 0.927 ± 0.019
0.46AsnPhe: 0.46 ± 0.016
1.927AsnGly: 1.927 ± 0.032
0.388AsnHis: 0.388 ± 0.012
0.55AsnIle: 0.55 ± 0.017
0.248AsnLys: 0.248 ± 0.011
1.435AsnLeu: 1.435 ± 0.028
0.275AsnMet: 0.275 ± 0.013
0.385AsnAsn: 0.385 ± 0.017
1.231AsnPro: 1.231 ± 0.026
0.464AsnGln: 0.464 ± 0.017
1.228AsnArg: 1.228 ± 0.026
0.834AsnSer: 0.834 ± 0.021
0.97AsnThr: 0.97 ± 0.026
1.364AsnVal: 1.364 ± 0.026
0.293AsnTrp: 0.293 ± 0.015
0.401AsnTyr: 0.401 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
9.724ProAla: 9.724 ± 0.099
0.366ProCys: 0.366 ± 0.013
4.651ProAsp: 4.651 ± 0.054
4.72ProGlu: 4.72 ± 0.046
1.643ProPhe: 1.643 ± 0.03
7.564ProGly: 7.564 ± 0.064
1.487ProHis: 1.487 ± 0.028
1.175ProIle: 1.175 ± 0.021
0.754ProLys: 0.754 ± 0.018
5.542ProLeu: 5.542 ± 0.059
1.031ProMet: 1.031 ± 0.02
0.816ProAsn: 0.816 ± 0.019
4.493ProPro: 4.493 ± 0.071
1.763ProGln: 1.763 ± 0.031
4.623ProArg: 4.623 ± 0.053
2.764ProSer: 2.764 ± 0.035
2.899ProThr: 2.899 ± 0.035
5.483ProVal: 5.483 ± 0.057
1.002ProTrp: 1.002 ± 0.024
1.19ProTyr: 1.19 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.561GlnAla: 3.561 ± 0.039
0.164GlnCys: 0.164 ± 0.008
1.527GlnAsp: 1.527 ± 0.027
1.624GlnGlu: 1.624 ± 0.029
0.703GlnPhe: 0.703 ± 0.018
2.133GlnGly: 2.133 ± 0.039
0.633GlnHis: 0.633 ± 0.019
1.0GlnIle: 1.0 ± 0.021
0.365GlnLys: 0.365 ± 0.014
2.995GlnLeu: 2.995 ± 0.038
0.442GlnMet: 0.442 ± 0.015
0.456GlnAsn: 0.456 ± 0.015
1.7GlnPro: 1.7 ± 0.036
1.201GlnGln: 1.201 ± 0.039
2.506GlnArg: 2.506 ± 0.044
1.136GlnSer: 1.136 ± 0.024
1.248GlnThr: 1.248 ± 0.025
2.199GlnVal: 2.199 ± 0.032
0.451GlnTrp: 0.451 ± 0.014
0.544GlnTyr: 0.544 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
11.297ArgAla: 11.297 ± 0.087
0.64ArgCys: 0.64 ± 0.016
4.546ArgAsp: 4.546 ± 0.045
5.116ArgGlu: 5.116 ± 0.056
2.307ArgPhe: 2.307 ± 0.032
6.696ArgGly: 6.696 ± 0.076
2.423ArgHis: 2.423 ± 0.032
3.174ArgIle: 3.174 ± 0.041
1.303ArgLys: 1.303 ± 0.028
9.767ArgLeu: 9.767 ± 0.081
1.784ArgMet: 1.784 ± 0.029
1.424ArgAsn: 1.424 ± 0.03
5.551ArgPro: 5.551 ± 0.062
2.568ArgGln: 2.568 ± 0.036
8.892ArgArg: 8.892 ± 0.079
4.088ArgSer: 4.088 ± 0.045
5.218ArgThr: 5.218 ± 0.052
6.274ArgVal: 6.274 ± 0.055
1.484ArgTrp: 1.484 ± 0.031
1.826ArgTyr: 1.826 ± 0.029
0.001ArgXaa: 0.001 ± 0.001
Ser
6.383SerAla: 6.383 ± 0.053
0.399SerCys: 0.399 ± 0.013
2.482SerAsp: 2.482 ± 0.037
2.322SerGlu: 2.322 ± 0.03
1.443SerPhe: 1.443 ± 0.029
5.692SerGly: 5.692 ± 0.054
1.002SerHis: 1.002 ± 0.02
1.293SerIle: 1.293 ± 0.023
0.623SerLys: 0.623 ± 0.018
4.594SerLeu: 4.594 ± 0.049
1.012SerMet: 1.012 ± 0.022
0.786SerAsn: 0.786 ± 0.018
3.095SerPro: 3.095 ± 0.04
1.13SerGln: 1.13 ± 0.026
3.733SerArg: 3.733 ± 0.042
2.435SerSer: 2.435 ± 0.039
2.607SerThr: 2.607 ± 0.036
3.935SerVal: 3.935 ± 0.045
0.927SerTrp: 0.927 ± 0.024
1.058SerTyr: 1.058 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
8.92ThrAla: 8.92 ± 0.066
0.433ThrCys: 0.433 ± 0.016
3.496ThrAsp: 3.496 ± 0.043
3.326ThrGlu: 3.326 ± 0.041
1.66ThrPhe: 1.66 ± 0.029
6.883ThrGly: 6.883 ± 0.064
1.171ThrHis: 1.171 ± 0.025
1.647ThrIle: 1.647 ± 0.028
0.762ThrLys: 0.762 ± 0.019
5.498ThrLeu: 5.498 ± 0.053
0.921ThrMet: 0.921 ± 0.02
0.911ThrAsn: 0.911 ± 0.021
3.774ThrPro: 3.774 ± 0.046
1.222ThrGln: 1.222 ± 0.026
3.995ThrArg: 3.995 ± 0.045
2.798ThrSer: 2.798 ± 0.034
3.493ThrThr: 3.493 ± 0.047
5.762ThrVal: 5.762 ± 0.058
0.962ThrTrp: 0.962 ± 0.021
1.2ThrTyr: 1.2 ± 0.022
0.0ThrXaa: 0.0 ± 0.0
Val
10.67ValAla: 10.67 ± 0.078
0.73ValCys: 0.73 ± 0.017
4.623ValAsp: 4.623 ± 0.047
4.507ValGlu: 4.507 ± 0.045
2.276ValPhe: 2.276 ± 0.034
6.173ValGly: 6.173 ± 0.058
2.068ValHis: 2.068 ± 0.034
2.766ValIle: 2.766 ± 0.04
1.122ValLys: 1.122 ± 0.027
9.622ValLeu: 9.622 ± 0.074
1.254ValMet: 1.254 ± 0.029
1.524ValAsn: 1.524 ± 0.031
5.516ValPro: 5.516 ± 0.057
1.917ValGln: 1.917 ± 0.03
7.173ValArg: 7.173 ± 0.061
4.074ValSer: 4.074 ± 0.049
5.544ValThr: 5.544 ± 0.052
7.273ValVal: 7.273 ± 0.069
1.1ValTrp: 1.1 ± 0.021
1.446ValTyr: 1.446 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.78TrpAla: 1.78 ± 0.027
0.166TrpCys: 0.166 ± 0.008
0.881TrpAsp: 0.881 ± 0.019
0.833TrpGlu: 0.833 ± 0.018
0.491TrpPhe: 0.491 ± 0.015
1.067TrpGly: 1.067 ± 0.025
0.456TrpHis: 0.456 ± 0.014
0.479TrpIle: 0.479 ± 0.016
0.218TrpLys: 0.218 ± 0.01
1.904TrpLeu: 1.904 ± 0.032
0.298TrpMet: 0.298 ± 0.01
0.415TrpAsn: 0.415 ± 0.015
0.889TrpPro: 0.889 ± 0.023
0.674TrpGln: 0.674 ± 0.017
1.508TrpArg: 1.508 ± 0.029
0.961TrpSer: 0.961 ± 0.022
0.999TrpThr: 0.999 ± 0.021
0.973TrpVal: 0.973 ± 0.022
0.347TrpTrp: 0.347 ± 0.015
0.349TrpTyr: 0.349 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.605TyrAla: 2.605 ± 0.036
0.183TyrCys: 0.183 ± 0.01
1.332TyrAsp: 1.332 ± 0.027
1.185TyrGlu: 1.185 ± 0.023
0.569TyrPhe: 0.569 ± 0.015
2.01TyrGly: 2.01 ± 0.031
0.419TyrHis: 0.419 ± 0.014
0.441TyrIle: 0.441 ± 0.016
0.209TyrLys: 0.209 ± 0.01
2.057TyrLeu: 2.057 ± 0.033
0.215TyrMet: 0.215 ± 0.01
0.368TyrAsn: 0.368 ± 0.014
1.05TyrPro: 1.05 ± 0.022
0.607TyrGln: 0.607 ± 0.016
1.928TyrArg: 1.928 ± 0.027
0.893TyrSer: 0.893 ± 0.019
1.098TyrThr: 1.098 ± 0.025
1.466TyrVal: 1.466 ± 0.026
0.368TyrTrp: 0.368 ± 0.014
0.459TyrTyr: 0.459 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.02XaaXaa: 0.02 ± 0.008
Statistics based on 7037 proteins (2289126 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski