Bacillus lacisalsi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W0H9X8|A0A2W0H9X8_9BACI 5-oxoprolinase subunit A OS=Bacillus lacisalsi OX=2045244 GN=pxpA PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.39KK3 pKa = 10.0LLAGAGVLAAAAVIAGGIYY22 pKa = 10.67AFTNDD27 pKa = 3.89SAPSAGPDD35 pKa = 3.24YY36 pKa = 10.16EE37 pKa = 4.57NPVFEE42 pKa = 4.96PVIADD47 pKa = 3.49PSVIRR52 pKa = 11.84GEE54 pKa = 4.15DD55 pKa = 3.84GYY57 pKa = 11.22FYY59 pKa = 10.91AYY61 pKa = 8.98GTEE64 pKa = 4.43DD65 pKa = 2.87NWGDD69 pKa = 3.54GMGSRR74 pKa = 11.84VVPIVRR80 pKa = 11.84SQNLTDD86 pKa = 3.26WDD88 pKa = 4.32YY89 pKa = 11.92VGEE92 pKa = 4.28AFQTKK97 pKa = 9.77PDD99 pKa = 3.32WKK101 pKa = 10.17EE102 pKa = 3.99DD103 pKa = 3.49GGIWAPDD110 pKa = 2.75IVYY113 pKa = 10.45FNDD116 pKa = 2.98QYY118 pKa = 11.73YY119 pKa = 10.75LYY121 pKa = 9.95YY122 pKa = 10.55SQSTWGDD129 pKa = 3.53PNPAIGVAVADD140 pKa = 4.75DD141 pKa = 3.85PAGPFDD147 pKa = 4.51DD148 pKa = 3.97QGKK151 pKa = 9.39LFDD154 pKa = 4.13SEE156 pKa = 4.78EE157 pKa = 4.06IGVPNSIDD165 pKa = 3.16PQLFIKK171 pKa = 10.58EE172 pKa = 4.51DD173 pKa = 3.43GTPYY177 pKa = 10.71LFWGSWYY184 pKa = 10.02GIWGIEE190 pKa = 4.09ISDD193 pKa = 4.63DD194 pKa = 3.31GLDD197 pKa = 3.63YY198 pKa = 11.34VGEE201 pKa = 4.21KK202 pKa = 10.33FQIAGTDD209 pKa = 3.8FEE211 pKa = 4.71APYY214 pKa = 10.24IIEE217 pKa = 4.49RR218 pKa = 11.84DD219 pKa = 3.37DD220 pKa = 3.34YY221 pKa = 11.46FYY223 pKa = 11.39FFGSKK228 pKa = 10.43GSCCEE233 pKa = 4.12GQFSEE238 pKa = 4.29YY239 pKa = 9.69RR240 pKa = 11.84VAVGRR245 pKa = 11.84SEE247 pKa = 5.41SFDD250 pKa = 3.54GPYY253 pKa = 10.21LDD255 pKa = 5.33KK256 pKa = 11.32DD257 pKa = 3.84GNEE260 pKa = 4.66LNDD263 pKa = 3.4SSGTVILSGGEE274 pKa = 4.02GFAGPGHH281 pKa = 6.16NAVITDD287 pKa = 4.22DD288 pKa = 5.39AGQDD292 pKa = 2.89WMIYY296 pKa = 9.75HH297 pKa = 7.74AIDD300 pKa = 5.44KK301 pKa = 6.7EE302 pKa = 4.8TPWIGSGVTRR312 pKa = 11.84RR313 pKa = 11.84PLMLDD318 pKa = 3.6RR319 pKa = 11.84IIWEE323 pKa = 4.65DD324 pKa = 3.27GWPQIEE330 pKa = 4.28GGVPGEE336 pKa = 4.43GSQEE340 pKa = 3.86GPAIEE345 pKa = 4.01QQ346 pKa = 3.25

Molecular weight: 37.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W0H5S2|A0A2W0H5S2_9BACI NERD domain-containing protein OS=Bacillus lacisalsi OX=2045244 GN=CR205_18490 PE=4 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.18RR13 pKa = 11.84KK14 pKa = 8.28KK15 pKa = 8.46VHH17 pKa = 5.5GFRR20 pKa = 11.84TRR22 pKa = 11.84MSTKK26 pKa = 9.32NGRR29 pKa = 11.84RR30 pKa = 11.84VLASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.73GRR40 pKa = 11.84KK41 pKa = 8.76VLSAA45 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3874

0

3874

1131585

26

3595

292.1

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.968 ± 0.042

0.7 ± 0.013

5.628 ± 0.043

8.05 ± 0.057

4.477 ± 0.03

7.62 ± 0.042

2.186 ± 0.017

6.426 ± 0.037

5.45 ± 0.044

9.485 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.883 ± 0.019

3.635 ± 0.03

3.902 ± 0.026

3.358 ± 0.026

5.072 ± 0.034

5.847 ± 0.03

5.57 ± 0.036

7.36 ± 0.032

1.105 ± 0.016

3.279 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski