Amino acid dipepetide frequency for Escherichia phage N30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.655AlaAla: 9.655 ± 1.267
0.854AlaCys: 0.854 ± 0.307
6.152AlaAsp: 6.152 ± 0.818
5.468AlaGlu: 5.468 ± 0.591
3.161AlaPhe: 3.161 ± 0.381
7.861AlaGly: 7.861 ± 0.924
1.88AlaHis: 1.88 ± 0.391
5.383AlaIle: 5.383 ± 0.758
6.494AlaLys: 6.494 ± 0.812
8.202AlaLeu: 8.202 ± 0.969
2.905AlaMet: 2.905 ± 0.512
4.272AlaAsn: 4.272 ± 0.523
2.392AlaPro: 2.392 ± 0.393
3.418AlaGln: 3.418 ± 0.739
5.554AlaArg: 5.554 ± 0.518
5.725AlaSer: 5.725 ± 0.767
3.589AlaThr: 3.589 ± 0.538
4.956AlaVal: 4.956 ± 0.754
1.794AlaTrp: 1.794 ± 0.424
2.82AlaTyr: 2.82 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
0.684CysAla: 0.684 ± 0.26
0.256CysCys: 0.256 ± 0.156
1.196CysAsp: 1.196 ± 0.407
0.427CysGlu: 0.427 ± 0.213
0.513CysPhe: 0.513 ± 0.227
0.684CysGly: 0.684 ± 0.28
0.684CysHis: 0.684 ± 0.255
0.598CysIle: 0.598 ± 0.223
0.598CysLys: 0.598 ± 0.254
0.598CysLeu: 0.598 ± 0.23
0.171CysMet: 0.171 ± 0.122
0.427CysAsn: 0.427 ± 0.228
0.427CysPro: 0.427 ± 0.175
0.427CysGln: 0.427 ± 0.185
0.427CysArg: 0.427 ± 0.205
0.684CysSer: 0.684 ± 0.236
0.256CysThr: 0.256 ± 0.159
0.769CysVal: 0.769 ± 0.299
0.513CysTrp: 0.513 ± 0.244
0.256CysTyr: 0.256 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
4.785AspAla: 4.785 ± 0.638
0.684AspCys: 0.684 ± 0.278
4.016AspAsp: 4.016 ± 0.746
4.87AspGlu: 4.87 ± 0.701
3.076AspPhe: 3.076 ± 0.536
5.554AspGly: 5.554 ± 0.591
0.684AspHis: 0.684 ± 0.248
3.674AspIle: 3.674 ± 0.416
4.528AspLys: 4.528 ± 0.692
4.357AspLeu: 4.357 ± 0.774
2.563AspMet: 2.563 ± 0.608
2.734AspAsn: 2.734 ± 0.451
3.332AspPro: 3.332 ± 0.597
1.794AspGln: 1.794 ± 0.37
2.905AspArg: 2.905 ± 0.654
4.357AspSer: 4.357 ± 0.684
3.503AspThr: 3.503 ± 0.492
4.87AspVal: 4.87 ± 0.441
1.111AspTrp: 1.111 ± 0.323
1.538AspTyr: 1.538 ± 0.385
0.0AspXaa: 0.0 ± 0.0
Glu
7.177GluAla: 7.177 ± 0.921
0.342GluCys: 0.342 ± 0.168
4.614GluAsp: 4.614 ± 0.744
6.75GluGlu: 6.75 ± 1.022
2.051GluPhe: 2.051 ± 0.37
5.895GluGly: 5.895 ± 0.796
1.623GluHis: 1.623 ± 0.369
3.589GluIle: 3.589 ± 0.407
3.674GluLys: 3.674 ± 0.559
6.75GluLeu: 6.75 ± 0.769
2.392GluMet: 2.392 ± 0.477
2.905GluAsn: 2.905 ± 0.414
1.452GluPro: 1.452 ± 0.444
3.589GluGln: 3.589 ± 1.037
4.016GluArg: 4.016 ± 0.623
4.443GluSer: 4.443 ± 0.497
3.93GluThr: 3.93 ± 0.492
4.785GluVal: 4.785 ± 0.73
1.025GluTrp: 1.025 ± 0.402
3.076GluTyr: 3.076 ± 0.502
0.0GluXaa: 0.0 ± 0.0
Phe
2.221PheAla: 2.221 ± 0.43
0.513PheCys: 0.513 ± 0.22
3.161PheAsp: 3.161 ± 0.551
2.82PheGlu: 2.82 ± 0.406
1.025PhePhe: 1.025 ± 0.277
3.589PheGly: 3.589 ± 0.459
0.684PheHis: 0.684 ± 0.3
1.88PheIle: 1.88 ± 0.423
2.478PheLys: 2.478 ± 0.425
2.905PheLeu: 2.905 ± 0.439
1.452PheMet: 1.452 ± 0.268
1.709PheAsn: 1.709 ± 0.505
1.623PhePro: 1.623 ± 0.458
1.282PheGln: 1.282 ± 0.249
2.051PheArg: 2.051 ± 0.572
1.794PheSer: 1.794 ± 0.399
2.392PheThr: 2.392 ± 0.371
1.794PheVal: 1.794 ± 0.388
0.427PheTrp: 0.427 ± 0.174
0.854PheTyr: 0.854 ± 0.276
0.0PheXaa: 0.0 ± 0.0
Gly
6.579GlyAla: 6.579 ± 1.012
1.367GlyCys: 1.367 ± 0.394
4.785GlyAsp: 4.785 ± 0.66
4.87GlyGlu: 4.87 ± 0.61
3.674GlyPhe: 3.674 ± 0.593
5.468GlyGly: 5.468 ± 0.787
1.367GlyHis: 1.367 ± 0.322
4.87GlyIle: 4.87 ± 0.702
5.297GlyLys: 5.297 ± 0.834
5.639GlyLeu: 5.639 ± 0.959
2.051GlyMet: 2.051 ± 0.503
2.82GlyAsn: 2.82 ± 0.566
1.367GlyPro: 1.367 ± 0.414
2.734GlyGln: 2.734 ± 0.434
4.956GlyArg: 4.956 ± 0.636
4.443GlySer: 4.443 ± 0.687
4.016GlyThr: 4.016 ± 0.737
3.93GlyVal: 3.93 ± 0.602
1.452GlyTrp: 1.452 ± 0.468
3.076GlyTyr: 3.076 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
1.794HisAla: 1.794 ± 0.429
0.256HisCys: 0.256 ± 0.134
1.196HisAsp: 1.196 ± 0.29
1.538HisGlu: 1.538 ± 0.358
0.769HisPhe: 0.769 ± 0.225
1.111HisGly: 1.111 ± 0.34
0.684HisHis: 0.684 ± 0.245
1.452HisIle: 1.452 ± 0.392
1.452HisLys: 1.452 ± 0.406
1.965HisLeu: 1.965 ± 0.5
0.684HisMet: 0.684 ± 0.246
0.769HisAsn: 0.769 ± 0.24
0.342HisPro: 0.342 ± 0.154
0.427HisGln: 0.427 ± 0.288
0.94HisArg: 0.94 ± 0.277
0.94HisSer: 0.94 ± 0.244
0.513HisThr: 0.513 ± 0.226
1.538HisVal: 1.538 ± 0.426
0.427HisTrp: 0.427 ± 0.184
0.769HisTyr: 0.769 ± 0.251
0.0HisXaa: 0.0 ± 0.0
Ile
4.357IleAla: 4.357 ± 0.504
0.598IleCys: 0.598 ± 0.192
3.93IleAsp: 3.93 ± 0.641
4.101IleGlu: 4.101 ± 0.699
0.94IlePhe: 0.94 ± 0.259
4.016IleGly: 4.016 ± 0.481
1.282IleHis: 1.282 ± 0.451
3.332IleIle: 3.332 ± 0.609
3.503IleLys: 3.503 ± 0.468
3.845IleLeu: 3.845 ± 0.517
1.196IleMet: 1.196 ± 0.318
2.905IleAsn: 2.905 ± 0.689
2.478IlePro: 2.478 ± 0.383
1.623IleGln: 1.623 ± 0.438
4.016IleArg: 4.016 ± 0.7
2.905IleSer: 2.905 ± 0.489
2.82IleThr: 2.82 ± 0.361
2.649IleVal: 2.649 ± 0.414
0.598IleTrp: 0.598 ± 0.207
1.794IleTyr: 1.794 ± 0.269
0.0IleXaa: 0.0 ± 0.0
Lys
7.861LysAla: 7.861 ± 0.962
0.513LysCys: 0.513 ± 0.242
3.161LysAsp: 3.161 ± 0.458
5.126LysGlu: 5.126 ± 0.851
2.392LysPhe: 2.392 ± 0.391
5.041LysGly: 5.041 ± 0.75
1.623LysHis: 1.623 ± 0.435
2.307LysIle: 2.307 ± 0.391
4.699LysLys: 4.699 ± 0.928
5.212LysLeu: 5.212 ± 0.768
2.051LysMet: 2.051 ± 0.411
2.734LysAsn: 2.734 ± 0.383
2.649LysPro: 2.649 ± 0.573
2.82LysGln: 2.82 ± 0.594
4.016LysArg: 4.016 ± 0.596
4.016LysSer: 4.016 ± 0.634
2.905LysThr: 2.905 ± 0.396
4.785LysVal: 4.785 ± 0.609
0.684LysTrp: 0.684 ± 0.221
2.392LysTyr: 2.392 ± 0.358
0.0LysXaa: 0.0 ± 0.0
Leu
8.715LeuAla: 8.715 ± 1.169
0.513LeuCys: 0.513 ± 0.291
4.956LeuAsp: 4.956 ± 0.61
6.237LeuGlu: 6.237 ± 0.797
2.221LeuPhe: 2.221 ± 0.499
4.614LeuGly: 4.614 ± 0.788
1.111LeuHis: 1.111 ± 0.352
3.759LeuIle: 3.759 ± 0.586
6.237LeuLys: 6.237 ± 0.607
5.126LeuLeu: 5.126 ± 0.685
2.563LeuMet: 2.563 ± 0.42
3.845LeuAsn: 3.845 ± 0.498
2.905LeuPro: 2.905 ± 0.517
3.93LeuGln: 3.93 ± 0.587
5.383LeuArg: 5.383 ± 0.769
4.357LeuSer: 4.357 ± 0.66
4.956LeuThr: 4.956 ± 0.674
3.589LeuVal: 3.589 ± 0.608
1.367LeuTrp: 1.367 ± 0.47
2.734LeuTyr: 2.734 ± 0.535
0.0LeuXaa: 0.0 ± 0.0
Met
3.589MetAla: 3.589 ± 0.504
0.256MetCys: 0.256 ± 0.189
2.051MetAsp: 2.051 ± 0.432
1.623MetGlu: 1.623 ± 0.398
1.367MetPhe: 1.367 ± 0.398
2.307MetGly: 2.307 ± 0.373
0.427MetHis: 0.427 ± 0.185
1.452MetIle: 1.452 ± 0.339
1.282MetLys: 1.282 ± 0.234
3.503MetLeu: 3.503 ± 0.534
0.854MetMet: 0.854 ± 0.261
1.452MetAsn: 1.452 ± 0.352
1.282MetPro: 1.282 ± 0.327
1.282MetGln: 1.282 ± 0.318
1.282MetArg: 1.282 ± 0.282
1.452MetSer: 1.452 ± 0.377
1.88MetThr: 1.88 ± 0.486
2.563MetVal: 2.563 ± 0.475
0.085MetTrp: 0.085 ± 0.087
0.598MetTyr: 0.598 ± 0.244
0.0MetXaa: 0.0 ± 0.0
Asn
3.418AsnAla: 3.418 ± 0.477
0.684AsnCys: 0.684 ± 0.201
2.82AsnAsp: 2.82 ± 0.497
2.905AsnGlu: 2.905 ± 0.581
1.709AsnPhe: 1.709 ± 0.501
4.614AsnGly: 4.614 ± 0.609
0.94AsnHis: 0.94 ± 0.338
2.734AsnIle: 2.734 ± 0.457
2.307AsnLys: 2.307 ± 0.435
3.161AsnLeu: 3.161 ± 0.512
1.196AsnMet: 1.196 ± 0.379
1.794AsnAsn: 1.794 ± 0.385
2.905AsnPro: 2.905 ± 0.556
1.452AsnGln: 1.452 ± 0.33
1.88AsnArg: 1.88 ± 0.434
2.307AsnSer: 2.307 ± 0.697
2.99AsnThr: 2.99 ± 0.812
2.734AsnVal: 2.734 ± 0.438
0.769AsnTrp: 0.769 ± 0.264
1.367AsnTyr: 1.367 ± 0.325
0.0AsnXaa: 0.0 ± 0.0
Pro
3.332ProAla: 3.332 ± 0.413
0.427ProCys: 0.427 ± 0.16
2.82ProAsp: 2.82 ± 0.517
4.272ProGlu: 4.272 ± 0.841
1.452ProPhe: 1.452 ± 0.34
0.598ProGly: 0.598 ± 0.206
0.769ProHis: 0.769 ± 0.27
1.623ProIle: 1.623 ± 0.3
2.307ProLys: 2.307 ± 0.536
2.221ProLeu: 2.221 ± 0.366
1.282ProMet: 1.282 ± 0.342
2.221ProAsn: 2.221 ± 0.431
0.513ProPro: 0.513 ± 0.197
1.025ProGln: 1.025 ± 0.29
1.623ProArg: 1.623 ± 0.4
2.478ProSer: 2.478 ± 0.444
1.88ProThr: 1.88 ± 0.371
2.051ProVal: 2.051 ± 0.382
0.598ProTrp: 0.598 ± 0.163
1.452ProTyr: 1.452 ± 0.339
0.0ProXaa: 0.0 ± 0.0
Gln
3.845GlnAla: 3.845 ± 0.865
0.256GlnCys: 0.256 ± 0.125
1.709GlnAsp: 1.709 ± 0.409
3.161GlnGlu: 3.161 ± 0.464
2.221GlnPhe: 2.221 ± 0.343
2.136GlnGly: 2.136 ± 0.391
0.256GlnHis: 0.256 ± 0.189
1.452GlnIle: 1.452 ± 0.456
2.307GlnLys: 2.307 ± 0.491
4.357GlnLeu: 4.357 ± 0.579
1.282GlnMet: 1.282 ± 0.296
1.111GlnAsn: 1.111 ± 0.345
1.196GlnPro: 1.196 ± 0.417
1.88GlnGln: 1.88 ± 0.38
2.221GlnArg: 2.221 ± 0.536
2.221GlnSer: 2.221 ± 0.543
1.965GlnThr: 1.965 ± 0.499
1.88GlnVal: 1.88 ± 0.531
0.854GlnTrp: 0.854 ± 0.215
1.196GlnTyr: 1.196 ± 0.391
0.0GlnXaa: 0.0 ± 0.0
Arg
5.383ArgAla: 5.383 ± 0.507
0.854ArgCys: 0.854 ± 0.256
4.016ArgAsp: 4.016 ± 0.628
4.785ArgGlu: 4.785 ± 0.858
2.221ArgPhe: 2.221 ± 0.39
3.076ArgGly: 3.076 ± 0.542
0.94ArgHis: 0.94 ± 0.3
2.99ArgIle: 2.99 ± 0.375
4.272ArgLys: 4.272 ± 0.769
4.699ArgLeu: 4.699 ± 0.55
1.709ArgMet: 1.709 ± 0.425
2.392ArgAsn: 2.392 ± 0.571
2.051ArgPro: 2.051 ± 0.267
1.794ArgGln: 1.794 ± 0.418
2.99ArgArg: 2.99 ± 0.456
3.589ArgSer: 3.589 ± 0.676
2.563ArgThr: 2.563 ± 0.326
3.503ArgVal: 3.503 ± 0.498
0.854ArgTrp: 0.854 ± 0.283
1.623ArgTyr: 1.623 ± 0.423
0.0ArgXaa: 0.0 ± 0.0
Ser
5.468SerAla: 5.468 ± 0.758
0.684SerCys: 0.684 ± 0.28
4.699SerAsp: 4.699 ± 0.605
2.99SerGlu: 2.99 ± 0.412
2.392SerPhe: 2.392 ± 0.4
5.725SerGly: 5.725 ± 1.008
1.282SerHis: 1.282 ± 0.329
2.82SerIle: 2.82 ± 0.449
3.589SerLys: 3.589 ± 0.599
3.93SerLeu: 3.93 ± 0.673
1.367SerMet: 1.367 ± 0.364
2.905SerAsn: 2.905 ± 0.465
1.623SerPro: 1.623 ± 0.441
1.965SerGln: 1.965 ± 0.507
2.734SerArg: 2.734 ± 0.502
2.905SerSer: 2.905 ± 0.533
3.759SerThr: 3.759 ± 0.649
4.101SerVal: 4.101 ± 0.576
0.94SerTrp: 0.94 ± 0.265
2.051SerTyr: 2.051 ± 0.482
0.0SerXaa: 0.0 ± 0.0
Thr
4.357ThrAla: 4.357 ± 0.594
0.684ThrCys: 0.684 ± 0.278
3.418ThrAsp: 3.418 ± 0.453
3.93ThrGlu: 3.93 ± 0.725
1.88ThrPhe: 1.88 ± 0.407
5.554ThrGly: 5.554 ± 0.74
0.94ThrHis: 0.94 ± 0.263
3.845ThrIle: 3.845 ± 0.641
4.785ThrLys: 4.785 ± 0.502
4.528ThrLeu: 4.528 ± 0.606
1.367ThrMet: 1.367 ± 0.358
1.88ThrAsn: 1.88 ± 0.43
2.563ThrPro: 2.563 ± 0.514
1.794ThrGln: 1.794 ± 0.398
2.649ThrArg: 2.649 ± 0.446
3.161ThrSer: 3.161 ± 0.676
2.563ThrThr: 2.563 ± 0.468
3.503ThrVal: 3.503 ± 0.573
0.513ThrTrp: 0.513 ± 0.228
1.196ThrTyr: 1.196 ± 0.251
0.0ThrXaa: 0.0 ± 0.0
Val
5.554ValAla: 5.554 ± 0.673
0.342ValCys: 0.342 ± 0.17
3.332ValAsp: 3.332 ± 0.537
4.443ValGlu: 4.443 ± 0.874
2.221ValPhe: 2.221 ± 0.527
4.016ValGly: 4.016 ± 0.639
1.196ValHis: 1.196 ± 0.307
3.332ValIle: 3.332 ± 0.469
4.101ValLys: 4.101 ± 0.789
3.845ValLeu: 3.845 ± 0.615
1.965ValMet: 1.965 ± 0.417
2.734ValAsn: 2.734 ± 0.536
2.82ValPro: 2.82 ± 0.51
2.307ValGln: 2.307 ± 0.472
3.93ValArg: 3.93 ± 0.468
3.418ValSer: 3.418 ± 0.587
4.87ValThr: 4.87 ± 0.55
4.443ValVal: 4.443 ± 0.611
1.111ValTrp: 1.111 ± 0.29
2.563ValTyr: 2.563 ± 0.521
0.0ValXaa: 0.0 ± 0.0
Trp
0.769TrpAla: 0.769 ± 0.316
0.256TrpCys: 0.256 ± 0.162
0.598TrpAsp: 0.598 ± 0.221
1.111TrpGlu: 1.111 ± 0.256
0.598TrpPhe: 0.598 ± 0.249
0.598TrpGly: 0.598 ± 0.236
0.598TrpHis: 0.598 ± 0.285
0.598TrpIle: 0.598 ± 0.248
1.367TrpLys: 1.367 ± 0.346
1.538TrpLeu: 1.538 ± 0.35
0.427TrpMet: 0.427 ± 0.167
1.367TrpAsn: 1.367 ± 0.34
0.171TrpPro: 0.171 ± 0.143
0.598TrpGln: 0.598 ± 0.292
0.94TrpArg: 0.94 ± 0.253
0.94TrpSer: 0.94 ± 0.304
1.282TrpThr: 1.282 ± 0.284
1.623TrpVal: 1.623 ± 0.527
0.513TrpTrp: 0.513 ± 0.167
0.171TrpTyr: 0.171 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.99TyrAla: 2.99 ± 0.501
0.256TyrCys: 0.256 ± 0.173
2.307TyrAsp: 2.307 ± 0.377
2.734TyrGlu: 2.734 ± 0.506
0.769TyrPhe: 0.769 ± 0.29
2.307TyrGly: 2.307 ± 0.432
0.598TyrHis: 0.598 ± 0.251
1.196TyrIle: 1.196 ± 0.304
1.709TyrLys: 1.709 ± 0.331
2.734TyrLeu: 2.734 ± 0.474
1.025TyrMet: 1.025 ± 0.273
1.709TyrAsn: 1.709 ± 0.321
0.854TyrPro: 0.854 ± 0.229
1.367TyrGln: 1.367 ± 0.505
1.794TyrArg: 1.794 ± 0.486
1.794TyrSer: 1.794 ± 0.406
2.478TyrThr: 2.478 ± 0.507
2.478TyrVal: 2.478 ± 0.568
0.427TyrTrp: 0.427 ± 0.211
0.684TyrTyr: 0.684 ± 0.246
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (11705 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski